In order to reveal the influence of forced ventilation on the dispersion of droplets ejected from roadheader-mounted external sprayer,the paper studies the air-flowing field and the droplet distribution under the cond...In order to reveal the influence of forced ventilation on the dispersion of droplets ejected from roadheader-mounted external sprayer,the paper studies the air-flowing field and the droplet distribution under the condition of gentle breeze and normal forced ventilation in heading face using the particle tracking technology of computational fluid dynamics(CFD).The results show that air-flowing tendency in the same section presents great comparability in the period of gentle breeze and forced ventilation,and the difference mainly embodies in the different wind velocity.The influence of ventilation on the dispersion of droplets is faint under the gentle breeze condition.The droplet can be evenly distributed around the cutting head.However,under the normal forced ventilation,a large number of droplets will drift to the return air side.At the same time,droplet clusters are predominantly presented in the lower part of windward side and the middle of the leeward side around the cutting head.In contrast,the droplet concentration in other parts around cutting head decreases a lot and the droplets are unable to form close-grained mist curtain.So the dust escape channel is formed.In addition,the simulation results also reveal that the disturbance of air flow on the droplet distribution can be effectively relieved when using ventilation duct with Coanda effect(VDCE).Field experiment results show that the dust suppression efficiency of total dust and respirable dust increases respectively by 10.5%and 9.3%when using VDCE,which proves that it can weaken the influence of airflow on droplet dispersion.展开更多
In applications involving,e.g.,panel data,images,genomics microarrays,etc.,trace regression models are useful tools.To address the high-dimensional issue of these applications,it is common to assume some sparsity prop...In applications involving,e.g.,panel data,images,genomics microarrays,etc.,trace regression models are useful tools.To address the high-dimensional issue of these applications,it is common to assume some sparsity property.For the case of the parameter matrix being simultaneously low rank and elements-wise sparse,we estimate the parameter matrix through the least-squares approach with the composite penalty combining the nuclear norm and the l1norm.We extend the existing analysis of the low-rank trace regression with i.i.d.errors to exponentialβ-mixing errors.The explicit convergence rate and the asymptotic properties of the proposed estimator are established.Simulations,as well as a real data application,are also carried out for illustration.展开更多
Background The primary reasons for local recurrence and therapeutic failure in the treatment of malignant gliomas are the invasion and interactions of tumor cells with surrounding normal brain cells. However, these tu...Background The primary reasons for local recurrence and therapeutic failure in the treatment of malignant gliomas are the invasion and interactions of tumor cells with surrounding normal brain cells. However, these tumor cells are hard to be visualized directly in histopathological preparations, or in experimental glioma models. Therefore, we developed an experimental human dual-color in vivo glioma model, which made tracking solitary invasive glioma cells possible, for the purpose of visualizing the interactions between red fluorescence labeled human glioma cells and host brain cells. This may offer references for further studying the roles of tumor microenvironment during glioma tissue remodeling. Methods Transgenic female C57BL/6 mice expressing enhanced green fluorescent protein (EGFP) were crossed with male Balb/c nude mice. Then sib mating was allowed to occur continuously in order to establish an inbred nude mice strain with 50% of their offspring that are EGFP positive. Human glioma cell lines U87-MG and SU3 were transfected with red fluorescent protein (RFP) gene, and a rat C6 glioma cell line was stained directly with CM-Dil, to establish three glioma cell lines emitting red fluorescence (SU3-RFP, U87-RFP, and C6-CM-Dil). Red fluorescence tumor cells were inoculated via intra-cerebral injection into caudate nucleus of the EGFP nude mice. Tumor-bearing mice were sacrificed when their clinical symptoms appeared, and the whole brain was harvested and snap frozen for further analysis. Confocal laser scanning microscopy was performed to monitor the mutual interactions between tumor cells and host brain cells. Results Almost all the essential tissues of the established EGFP athymic Balb/c nude mice, except hair and erythrocytes, fluoresced green under excitation using a blue light-emitting flashlight with a central peak of 470 nm, approximately 50% of the offsprings were nu/nu EGFP+. SU3-RFP, U87-RFP, and C6-CM-Dil almost 100% expressed red fluorescence under the fluorescence microscope. Under fluorescence microscopic view, RFP+ cells were observed growing wherever they arrived at, locating in the brain parenchyma, ventricles, and para-vascular region. The interactions between the transplanted tumor cells and host adjacent cells could be classified into three types: (1) interweaving; (2) mergence; and (3) fusion. Interweaving was observed in the early stage of tumor remodeling, in which both transplantable tumor cells and host cells were observed scattered in the tumor invading and spreading area without organic connections. Mergence was defined as mutual interactions between tumor cells and host stroma during tumorigenesis. Direct cell fusion between transplantable tumor cells and host cells could be observed occasionally. Conclusions This study showed that self-established EGFP athymic nude mice offered the possibility of visualizing tumorigenesis of human xenograft tumor, and the dual-color xenograft glioma model was of considerable utility in studying the process of tumor remodeling. Based on this platform, mutual interactions between glioma cells and host tissues could be observed directly to further elucidate the development of tumor microenvironment.展开更多
A computational fluid dynamics model is used for the simulation of laminar flow ofwater-Al2O3 nanofluid in a confined slot impinging jet. The (steady-state and two-dimensional) Eulerian-Lagrangian model is used cons...A computational fluid dynamics model is used for the simulation of laminar flow ofwater-Al2O3 nanofluid in a confined slot impinging jet. The (steady-state and two-dimensional) Eulerian-Lagrangian model is used considering fluid-particle and particle-wall interactions (i.e., two-way coupling). A collocated grid and the SIMPLE algorithm are used for the coupling of pressure and velocity fields. The deposition model is used to investigate the effect of particle deposition on the impingement surface. Results indicate that the particle trajectory becomes stable farther from the jet with a rising Reynolds number and jet- impingement surface distance ratio. The heat transfer coefficient of the mixture model is higher than that of the Eulerian-Lagrangican model.展开更多
Rice-paddies are regarded as one of the main agricultural sources of N 2O and NO emissions. To date, however, specific N2O and NO production pathways are poorly understood in paddy soils. ^15N-tracing experiments were...Rice-paddies are regarded as one of the main agricultural sources of N 2O and NO emissions. To date, however, specific N2O and NO production pathways are poorly understood in paddy soils. ^15N-tracing experiments were carded out to investigate the processes responsible for N2O and NO production in two paddy soils with substantially different soil properties. Laboratory incubation experiments were carried out under aerobic conditions at moisture contents corresponding to 60% of water holding capacity. The relative importance of nitrification and denitrification to the flux of NaO was quantified by periodically measuring and comparing the enrichments of the N2O, NH^-N and NO3-N pools. The results showed that both N2O and NO emission rates in an alkaline paddy soil with clayey texture were substantially higher than those in a neutral paddy soil with silty loamy texture. In accordance with most published results, the ammonium N pool was the main source of N2O emission across the soil profiles of the two paddy soils, being responsible for 59.7% to 97.7% of total N2O emissions. The NO3-N pool of N2O emission was relatively less important under the given aerobic conditions. The rates of N2O emission from nitrification (N2On) among different soil layers were significantly different, which could be attributed to both the differences in gross N nitrification rates and to the ratios of nitrified N emitted as NzO among soil layers. Furthermore, NO fluxes were positively correlated with the changes in gross nitrification rates and the ratios of NO/N2O in the two paddy soils were always greater than one (from 1.26 to 6.47). We therefore deduce that, similar to N2O, nitrification was also the dominant source of NO in the tested paddy soils at water contents below 60% water holding capacity.展开更多
Although to date individual gross N transformations could be quantified by 15N tracing method and models, studies are still limited in paddy soil. An incubation experiment was conducted using topsoil (0-20 cm) and s...Although to date individual gross N transformations could be quantified by 15N tracing method and models, studies are still limited in paddy soil. An incubation experiment was conducted using topsoil (0-20 cm) and subsoil (20-60 cm) of two paddy soils, alkaline and clay (AC) soil and neutral and silt loam (NSL) soil, to investigate gross N transformation rates. Soil samples were labeled with either 15NHaNO3 or NH4SN03, and then incubated at 25 ℃for 168 h at 60% water-holding capacity. The gross N mineralization (recalcitrant and labile organic N mineralization) rates in AC soil were 1.6 to 3.3 times higher than that in NSL soil, and the gross N nitrification (autotrophic and heterotrophic nitrification) rates in AC soil were 2.4 to 4.4 times higher than those in NSL soil. Although gross NO3 consumption (i.e., NO3 immobilization and dissimilatory NO3 reduction to NH+) rates increased with increasing gross nitrification rates, the measured net nitrification rate in AC soil was approximately 2.0 to 5.1 times higher than that in NSL soil. These showed that high NO3 production capacity of alkaline paddy soil should be a cause for concern because an accumulation of NO3 can increase the risk of NO3 loss through leaching and denitrification.展开更多
Experimental and geochemical modelling studies were carried out to identify mineral and solid phases containing major, minor, and trace elements and the mechanism of the retention of these elements in Flue Gas Desulph...Experimental and geochemical modelling studies were carried out to identify mineral and solid phases containing major, minor, and trace elements and the mechanism of the retention of these elements in Flue Gas Desulphurisation(FGD)-gypsum samples from a coal-fired power plant under filtered water recirculation to the scrubber and forced oxidation conditions. The role of the p H and related environmental factors on the mobility of Li, Ni, Zn, As, Se, Mo, and U from FGD-gypsums for a comprehensive assessment of element leaching behaviour were also carried out. Results show that the extraction rate of the studied elements generally increases with decreasing the p H value of the FGD-gypsum leachates. The increase of the mobility of elements such as U, Se, and As in the FGD-gypsum entails the modification of their aqueous speciation in the leachates; UO2SO4, H2 Se, and HAs O2 are the aqueous complexes with the highest activities under acidic conditions.The speciation of Zn, Li, and Ni is not affected in spite of p H changes; these elements occur as free cations and associated to SO4 ^2 in the FGD-gypsum leachates. The mobility of Cu and Mo decreases by decreasing the p H of the FGD-gypsum leachates, which might be associated to the precipitation of Cu Se2 and Mo Se2, respectively. Time-of-Flight mass spectrometry of the solid phase combined with geochemical modelling of the aqueous phase has proved useful in understanding the mobility and geochemical behaviour of elements and their partitioning into FGD-gypsum samples.展开更多
基金supported by the Program for Postgraduates Research Innovation in University of Jiangsu Province of China (No.CXLX13_955)the National Natural Science Foundation of China (No.51104153)
文摘In order to reveal the influence of forced ventilation on the dispersion of droplets ejected from roadheader-mounted external sprayer,the paper studies the air-flowing field and the droplet distribution under the condition of gentle breeze and normal forced ventilation in heading face using the particle tracking technology of computational fluid dynamics(CFD).The results show that air-flowing tendency in the same section presents great comparability in the period of gentle breeze and forced ventilation,and the difference mainly embodies in the different wind velocity.The influence of ventilation on the dispersion of droplets is faint under the gentle breeze condition.The droplet can be evenly distributed around the cutting head.However,under the normal forced ventilation,a large number of droplets will drift to the return air side.At the same time,droplet clusters are predominantly presented in the lower part of windward side and the middle of the leeward side around the cutting head.In contrast,the droplet concentration in other parts around cutting head decreases a lot and the droplets are unable to form close-grained mist curtain.So the dust escape channel is formed.In addition,the simulation results also reveal that the disturbance of air flow on the droplet distribution can be effectively relieved when using ventilation duct with Coanda effect(VDCE).Field experiment results show that the dust suppression efficiency of total dust and respirable dust increases respectively by 10.5%and 9.3%when using VDCE,which proves that it can weaken the influence of airflow on droplet dispersion.
基金supported by the NSF of China(Grant No.12201259)supported by NSF of China(Grant No.11971208)+7 种基金supported by the NSF of China(Grant No.12201260)Jiangxi Provincial NSF(Grant No.20224BAB211008)Jiangxi Provincial NSF(Grant No.20212BAB211010)Science and Technology research project of the Education Department of Jiangxi Province(Grant No.GJJ2200537)Science and Technology Research Project of the Education Department of Jiangxi Province(Grant No.GJJ200545)NSSF of China(Grant No.21&ZD152)NSSF of China(Grant No.20BTJ008)China Postdoctoral Science Foundation(Grant No.2022M711425)。
文摘In applications involving,e.g.,panel data,images,genomics microarrays,etc.,trace regression models are useful tools.To address the high-dimensional issue of these applications,it is common to assume some sparsity property.For the case of the parameter matrix being simultaneously low rank and elements-wise sparse,we estimate the parameter matrix through the least-squares approach with the composite penalty combining the nuclear norm and the l1norm.We extend the existing analysis of the low-rank trace regression with i.i.d.errors to exponentialβ-mixing errors.The explicit convergence rate and the asymptotic properties of the proposed estimator are established.Simulations,as well as a real data application,are also carried out for illustration.
基金This study was supported by grants from the National Natural Science Foundation of China (No. 81071766, No. 81101909, and No. 81172400), the National Basic Research Program of China ("973" Program, No. 2010CB529403), and the Natural Science Foundation of Jiangsu, China (No. BK2010227).
文摘Background The primary reasons for local recurrence and therapeutic failure in the treatment of malignant gliomas are the invasion and interactions of tumor cells with surrounding normal brain cells. However, these tumor cells are hard to be visualized directly in histopathological preparations, or in experimental glioma models. Therefore, we developed an experimental human dual-color in vivo glioma model, which made tracking solitary invasive glioma cells possible, for the purpose of visualizing the interactions between red fluorescence labeled human glioma cells and host brain cells. This may offer references for further studying the roles of tumor microenvironment during glioma tissue remodeling. Methods Transgenic female C57BL/6 mice expressing enhanced green fluorescent protein (EGFP) were crossed with male Balb/c nude mice. Then sib mating was allowed to occur continuously in order to establish an inbred nude mice strain with 50% of their offspring that are EGFP positive. Human glioma cell lines U87-MG and SU3 were transfected with red fluorescent protein (RFP) gene, and a rat C6 glioma cell line was stained directly with CM-Dil, to establish three glioma cell lines emitting red fluorescence (SU3-RFP, U87-RFP, and C6-CM-Dil). Red fluorescence tumor cells were inoculated via intra-cerebral injection into caudate nucleus of the EGFP nude mice. Tumor-bearing mice were sacrificed when their clinical symptoms appeared, and the whole brain was harvested and snap frozen for further analysis. Confocal laser scanning microscopy was performed to monitor the mutual interactions between tumor cells and host brain cells. Results Almost all the essential tissues of the established EGFP athymic Balb/c nude mice, except hair and erythrocytes, fluoresced green under excitation using a blue light-emitting flashlight with a central peak of 470 nm, approximately 50% of the offsprings were nu/nu EGFP+. SU3-RFP, U87-RFP, and C6-CM-Dil almost 100% expressed red fluorescence under the fluorescence microscope. Under fluorescence microscopic view, RFP+ cells were observed growing wherever they arrived at, locating in the brain parenchyma, ventricles, and para-vascular region. The interactions between the transplanted tumor cells and host adjacent cells could be classified into three types: (1) interweaving; (2) mergence; and (3) fusion. Interweaving was observed in the early stage of tumor remodeling, in which both transplantable tumor cells and host cells were observed scattered in the tumor invading and spreading area without organic connections. Mergence was defined as mutual interactions between tumor cells and host stroma during tumorigenesis. Direct cell fusion between transplantable tumor cells and host cells could be observed occasionally. Conclusions This study showed that self-established EGFP athymic nude mice offered the possibility of visualizing tumorigenesis of human xenograft tumor, and the dual-color xenograft glioma model was of considerable utility in studying the process of tumor remodeling. Based on this platform, mutual interactions between glioma cells and host tissues could be observed directly to further elucidate the development of tumor microenvironment.
文摘A computational fluid dynamics model is used for the simulation of laminar flow ofwater-Al2O3 nanofluid in a confined slot impinging jet. The (steady-state and two-dimensional) Eulerian-Lagrangian model is used considering fluid-particle and particle-wall interactions (i.e., two-way coupling). A collocated grid and the SIMPLE algorithm are used for the coupling of pressure and velocity fields. The deposition model is used to investigate the effect of particle deposition on the impingement surface. Results indicate that the particle trajectory becomes stable farther from the jet with a rising Reynolds number and jet- impingement surface distance ratio. The heat transfer coefficient of the mixture model is higher than that of the Eulerian-Lagrangican model.
基金supported by the Sino-German collaborative project:"Innovative nitrogen management technologies to improve agricultural production and environmental protection in intensive Chinese agriculture"co-funded by the Chinese Ministry of Science and Technology(MOST grant no.2007DFA30850)the German Ministry of Education and Research(BMBF FKZ:0330800C)
文摘Rice-paddies are regarded as one of the main agricultural sources of N 2O and NO emissions. To date, however, specific N2O and NO production pathways are poorly understood in paddy soils. ^15N-tracing experiments were carded out to investigate the processes responsible for N2O and NO production in two paddy soils with substantially different soil properties. Laboratory incubation experiments were carried out under aerobic conditions at moisture contents corresponding to 60% of water holding capacity. The relative importance of nitrification and denitrification to the flux of NaO was quantified by periodically measuring and comparing the enrichments of the N2O, NH^-N and NO3-N pools. The results showed that both N2O and NO emission rates in an alkaline paddy soil with clayey texture were substantially higher than those in a neutral paddy soil with silty loamy texture. In accordance with most published results, the ammonium N pool was the main source of N2O emission across the soil profiles of the two paddy soils, being responsible for 59.7% to 97.7% of total N2O emissions. The NO3-N pool of N2O emission was relatively less important under the given aerobic conditions. The rates of N2O emission from nitrification (N2On) among different soil layers were significantly different, which could be attributed to both the differences in gross N nitrification rates and to the ratios of nitrified N emitted as NzO among soil layers. Furthermore, NO fluxes were positively correlated with the changes in gross nitrification rates and the ratios of NO/N2O in the two paddy soils were always greater than one (from 1.26 to 6.47). We therefore deduce that, similar to N2O, nitrification was also the dominant source of NO in the tested paddy soils at water contents below 60% water holding capacity.
基金supported by the Ministry of Science and Technology of China(No.2007DFA30850)the German Ministry of Education and Research(No.0330800C)a German DAAD-PPP Project(No.50751522)joint with the China Scholarship Council(No.2011016097)
文摘Although to date individual gross N transformations could be quantified by 15N tracing method and models, studies are still limited in paddy soil. An incubation experiment was conducted using topsoil (0-20 cm) and subsoil (20-60 cm) of two paddy soils, alkaline and clay (AC) soil and neutral and silt loam (NSL) soil, to investigate gross N transformation rates. Soil samples were labeled with either 15NHaNO3 or NH4SN03, and then incubated at 25 ℃for 168 h at 60% water-holding capacity. The gross N mineralization (recalcitrant and labile organic N mineralization) rates in AC soil were 1.6 to 3.3 times higher than that in NSL soil, and the gross N nitrification (autotrophic and heterotrophic nitrification) rates in AC soil were 2.4 to 4.4 times higher than those in NSL soil. Although gross NO3 consumption (i.e., NO3 immobilization and dissimilatory NO3 reduction to NH+) rates increased with increasing gross nitrification rates, the measured net nitrification rate in AC soil was approximately 2.0 to 5.1 times higher than that in NSL soil. These showed that high NO3 production capacity of alkaline paddy soil should be a cause for concern because an accumulation of NO3 can increase the risk of NO3 loss through leaching and denitrification.
文摘Experimental and geochemical modelling studies were carried out to identify mineral and solid phases containing major, minor, and trace elements and the mechanism of the retention of these elements in Flue Gas Desulphurisation(FGD)-gypsum samples from a coal-fired power plant under filtered water recirculation to the scrubber and forced oxidation conditions. The role of the p H and related environmental factors on the mobility of Li, Ni, Zn, As, Se, Mo, and U from FGD-gypsums for a comprehensive assessment of element leaching behaviour were also carried out. Results show that the extraction rate of the studied elements generally increases with decreasing the p H value of the FGD-gypsum leachates. The increase of the mobility of elements such as U, Se, and As in the FGD-gypsum entails the modification of their aqueous speciation in the leachates; UO2SO4, H2 Se, and HAs O2 are the aqueous complexes with the highest activities under acidic conditions.The speciation of Zn, Li, and Ni is not affected in spite of p H changes; these elements occur as free cations and associated to SO4 ^2 in the FGD-gypsum leachates. The mobility of Cu and Mo decreases by decreasing the p H of the FGD-gypsum leachates, which might be associated to the precipitation of Cu Se2 and Mo Se2, respectively. Time-of-Flight mass spectrometry of the solid phase combined with geochemical modelling of the aqueous phase has proved useful in understanding the mobility and geochemical behaviour of elements and their partitioning into FGD-gypsum samples.