A kind of functional liquid was extracted from compound Chinese traditional medicine over 20 different varieties such as ginseng, danggui and so on. The anti-anoxic effects were observed by the ways of oxygen lack in ...A kind of functional liquid was extracted from compound Chinese traditional medicine over 20 different varieties such as ginseng, danggui and so on. The anti-anoxic effects were observed by the ways of oxygen lack in normal atmosphere of mice, decapitation and erythrocyte count and hemoglobin in the mice. Experiments done in this study included observing the best dose and the best time of appearing anti-anoxic effects in mice. Results were found that the functional liquid could prolong not only the survival time and the brain survival time of the mouse, but also increasing the number of erythrocytes (RBC) and the content of hemoglobin (Hb); and the functional liquid has markedly protecting cardio-cerebral function; If the dosage of the functional liquid control on the 0.3ml/day for each mouse, the best effects should appear after four hour. And the functional liquid had no obvious effect on the weight of the mice within thirty days.展开更多
[Objective]This paper aimed to study the anti-bacterial effect of traditional Chinese medicine on cow recessive mastitis＇ s main pathogenic bacteria and provide basis for its clinical application.[Method]Plating and ...[Objective]This paper aimed to study the anti-bacterial effect of traditional Chinese medicine on cow recessive mastitis＇ s main pathogenic bacteria and provide basis for its clinical application.[Method]Plating and test-tube methods were used to determine the anti-bacterial diameter,minimum inhibitory concentration（MIC）and minimum bactericidal concentration（MBC）of 20 kinds of traditional Chinese medicine on dairy cow recessive mastitis＇ s clinically isolated main pathogenic bacteria,Staphylococcus aureus and Streptococcus agalactiae.[Conclusion]The results indicated that 7 kinds of traditional Chinese medicine,Herba Taraxaci,Rhizoma Coptidis,Fructus Forsythiae,Herba Andrographis,Radix Scutellar iae,Flos Carthami,Flos Chrysanthemi Indici had strong anti-bacterial effect.展开更多
Dengue virus(DENV) has emerged as a major virus that is spread by mosquitoes. Recently, it has spread to more than a hundred nations but continues to lack specific treatable medication. Many traditional Chinese medici...Dengue virus(DENV) has emerged as a major virus that is spread by mosquitoes. Recently, it has spread to more than a hundred nations but continues to lack specific treatable medication. Many traditional Chinese medicinal(TCM) plants are in practice for dengue fever in dengue endemic regions. These traditional medicines persevere with treatments, which modern medicines lack. The study aims to substantiate the anti-dengue potential of some traditional herbs and make them available for further studies to facilitate TCM users. Twelve TCM plants aqueous extracts were evaluated, which are described as cool herbs used for the diseases with high fever. Lead plants were established through detailed in vitro foci forming unit reduction analysis(FFURA) against all four serotypes and validated through quantitative real-time RT-PCR(q RT-PCR). Four plants potentially inhibited the virus in primary phenotypic in vitro evaluation. Two lead plants Dryopteris crassirhizoma(DC) and Morus alba(MA) were identified with half minimal inhibitory concentration(IC50) 130 and 221 μg m L^-1, respectively, while the selectivity indices(SI) were 4.21 and 4.62, respectively. Lead plants equally inhibited all four serotypes of DENV. Time-of-addition analysis suggested that, DC was active at later stages of viral replication, whereas MA was active during the early stages and even showed some prophylactic activity. Liquid chromatography-mass spectrometry(HPLC/MS) analysis revealed presence of flavonoids. DC and MA are identified as potential anti-dengue plants, active against varied stages of dengue virus replication cycle. These results may serve as the base knowledge for further investigation on their combined treatments or integrative treatment with western medicines, which may improve the overall anti-dengue activity in future.展开更多
Colorectal cancer remains the third most common malignancy worldwide. According to the principles of Traditional Chinese Medicine (TCM), colorectal cancer is related to spleen-deficiency, damp-heat, and toxicity accum...Colorectal cancer remains the third most common malignancy worldwide. According to the principles of Traditional Chinese Medicine (TCM), colorectal cancer is related to spleen-deficiency, damp-heat, and toxicity accumulation. Traditional Chinese medicine has been confirmed to effectively reduce toxic side effects and enhance curative effects of chemotherapy, palliate clinical syndrome, prevent recurrence and metastasis, improve quality of life and immune function, and prolong survival time in colorectal cancer. However, TCM theoretical and syndrome differentiation study has lagged far behind due to progression of therapy model in colorectal cancer. As a type of drug treatment, there are some common biological basis between chemotherapy, targeted therapy and anti-cancer herb therapy in colorectal cancer, such as apoptosis, cell senescence and autophagy. There are growing needs to explore the effect mechanism and compatibility of anti-cancer herbs, to further enhance the efficacy of TCM treatment in colorectal cancer.展开更多
Fluorescent silicon （Si） nanopartides （SiNPs） hold great promise for innumerable biological and biomedical applications owing to their unique optical properties and negligible toxicity. In this article, we present...Fluorescent silicon （Si） nanopartides （SiNPs） hold great promise for innumerable biological and biomedical applications owing to their unique optical properties and negligible toxicity. In this article, we present a new traditional Chinese medicine （TCM） molecule-assisted chemical synthetic strateg36 suitable for the production of multifunctional small-sized （diameter： - 3.7 nm） SiNPs in a facile and rapid （- 10 min） manner. Of particular significance, the resultant SiNPs simultaneously exhibited robust and stable fluorescence （photoluminescence quantum yield （PLQY）： ~ 15%）, as well as intrinsic anti-cancer efficacy with excellent selectivity toward cancer cells. Taking advantage of these unique merits, we further employed these novel fluorescent anti-cancer SiNPs （AC-SiNPs） for the fluorescence tracking and treatment of tumors, demonstrating long-term （~ 18 days） inhibition of tumor growth in tumor-bearing mice. Consequently, we believe this new TCM-assisted chemical synthetic method is highly attractive for designing silicon nanostructures featuring multiple functionalities, and we suggest these AC-SiNPs as novel promising tools for providing visual evidence of TCM-based cancer treatment.展开更多
Objective: To observe the short￣term efficacy and safety of Shenqi mixture (参芪合剂, SQM) combined with microwave coagulation in treating primary hepatocellular carcinoma (HCC).Methods: Seventy-two patients with pri...Objective: To observe the short￣term efficacy and safety of Shenqi mixture (参芪合剂, SQM) combined with microwave coagulation in treating primary hepatocellular carcinoma (HCC).Methods: Seventy-two patients with primary HCC of stage Ⅱ-Ⅲ, Karnofsky scoring ≥50 scores and predicted survival period ≥3 months were selected and randomly assigned into two groups, the treated group and the control group, 36 in each. Microwave therapy was applied to both groups by double leads, 60 W, 800 sec once a week for two weeks. To the treated group, SQM was given additionally through oral intake of 20 ml, three times a day for 1 month. The changes in tumor size, main symptoms, serum level of alpha-fetoprotein (AFP), immune function and adverse reaction were observed after treatment and the immune parameters of the patients were compared with 30 healthy persons in the normal control group. Results: (1) In the SQM treated group, after treatment 3 patients got completely remitted (CR), 24 partial remitted (PR), 4 unchanged (NC) and 5 progressively deteriorated (PD), the effective rate being 75.00%; while in the control group, 1 got CR, 19 PR, 9 NC and 7 PD, the effective rate being 55.56%. Comparison of the effective rate between the two groups showed significant difference (P<0.05). (2) AFP level decreased after treatment in both groups, but the decrement in the treated group was significantly higher than that in the control group (P<0.01). (3) After treatment, in the treated group, CD3+, CD4+, CD4+/CD8+ and NK activity were improved, Karnofsky scores increased and liver function bettered, with these improvements significantly superior to those in the control group (P<0.01). (4) The improvement in symptoms such as hepatic region pain, fever, weakness, poor appetite and jaundice in the treated group after treatment was also superior to that in the control group (P<0.01). (5)The 12-month, 18-month and 24-month survival rates were higher and the recurrence rate was lower in the treated group than those in the control group, showing significant difference (P<0.05). Conclusion: Combined therapy with SQM and microwave coagulation could not only kill the tumor and residue tumor cells to prevent recurrence, but also enhance the cellular immunity of organism. It is one of the effective therapies for patients with middle-advanced hepatocarcinoma, who have lost the chance of surgical operation. It could improve clinical symptoms, elevate the quality of life, prolong the survival period of patients, but shows no evident adverse reaction.展开更多
文摘A kind of functional liquid was extracted from compound Chinese traditional medicine over 20 different varieties such as ginseng, danggui and so on. The anti-anoxic effects were observed by the ways of oxygen lack in normal atmosphere of mice, decapitation and erythrocyte count and hemoglobin in the mice. Experiments done in this study included observing the best dose and the best time of appearing anti-anoxic effects in mice. Results were found that the functional liquid could prolong not only the survival time and the brain survival time of the mouse, but also increasing the number of erythrocytes (RBC) and the content of hemoglobin (Hb); and the functional liquid has markedly protecting cardio-cerebral function; If the dosage of the functional liquid control on the 0.3ml/day for each mouse, the best effects should appear after four hour. And the functional liquid had no obvious effect on the weight of the mice within thirty days.
基金Supported by Shijiazhuang Science and Technology Development Plan Project (08150132A)The Ministry of Science and Technology Spark Plan (2012GA6200025)
文摘[Objective]This paper aimed to study the anti-bacterial effect of traditional Chinese medicine on cow recessive mastitis＇ s main pathogenic bacteria and provide basis for its clinical application.[Method]Plating and test-tube methods were used to determine the anti-bacterial diameter,minimum inhibitory concentration（MIC）and minimum bactericidal concentration（MBC）of 20 kinds of traditional Chinese medicine on dairy cow recessive mastitis＇ s clinically isolated main pathogenic bacteria,Staphylococcus aureus and Streptococcus agalactiae.[Conclusion]The results indicated that 7 kinds of traditional Chinese medicine,Herba Taraxaci,Rhizoma Coptidis,Fructus Forsythiae,Herba Andrographis,Radix Scutellar iae,Flos Carthami,Flos Chrysanthemi Indici had strong anti-bacterial effect.
基金the Universiti Tunku Abdul Rahman,Malaysia,in providing funding to this project through UTARSRF strategic research funding scheme IPRS/RMC/UTARSRF/PROGRAMME 2014-C1/007。
文摘Dengue virus(DENV) has emerged as a major virus that is spread by mosquitoes. Recently, it has spread to more than a hundred nations but continues to lack specific treatable medication. Many traditional Chinese medicinal(TCM) plants are in practice for dengue fever in dengue endemic regions. These traditional medicines persevere with treatments, which modern medicines lack. The study aims to substantiate the anti-dengue potential of some traditional herbs and make them available for further studies to facilitate TCM users. Twelve TCM plants aqueous extracts were evaluated, which are described as cool herbs used for the diseases with high fever. Lead plants were established through detailed in vitro foci forming unit reduction analysis(FFURA) against all four serotypes and validated through quantitative real-time RT-PCR(q RT-PCR). Four plants potentially inhibited the virus in primary phenotypic in vitro evaluation. Two lead plants Dryopteris crassirhizoma(DC) and Morus alba(MA) were identified with half minimal inhibitory concentration(IC50) 130 and 221 μg m L^-1, respectively, while the selectivity indices(SI) were 4.21 and 4.62, respectively. Lead plants equally inhibited all four serotypes of DENV. Time-of-addition analysis suggested that, DC was active at later stages of viral replication, whereas MA was active during the early stages and even showed some prophylactic activity. Liquid chromatography-mass spectrometry(HPLC/MS) analysis revealed presence of flavonoids. DC and MA are identified as potential anti-dengue plants, active against varied stages of dengue virus replication cycle. These results may serve as the base knowledge for further investigation on their combined treatments or integrative treatment with western medicines, which may improve the overall anti-dengue activity in future.
文摘Colorectal cancer remains the third most common malignancy worldwide. According to the principles of Traditional Chinese Medicine (TCM), colorectal cancer is related to spleen-deficiency, damp-heat, and toxicity accumulation. Traditional Chinese medicine has been confirmed to effectively reduce toxic side effects and enhance curative effects of chemotherapy, palliate clinical syndrome, prevent recurrence and metastasis, improve quality of life and immune function, and prolong survival time in colorectal cancer. However, TCM theoretical and syndrome differentiation study has lagged far behind due to progression of therapy model in colorectal cancer. As a type of drug treatment, there are some common biological basis between chemotherapy, targeted therapy and anti-cancer herb therapy in colorectal cancer, such as apoptosis, cell senescence and autophagy. There are growing needs to explore the effect mechanism and compatibility of anti-cancer herbs, to further enhance the efficacy of TCM treatment in colorectal cancer.
基金We thank Prof. Shuit-Tong Lee （Soochow University, China） for general help and valuable suggestions. We appreciate financial support from the National Basic Research Program of China （No. 2013CB934400）, the National Natural Science Foundation of China （Nos. 61361160412, 31400860, 21575096, and 21605109）, and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions （PAPD）, 111 Project as well as Collaborative Innovation Center of Suzhou Nano Science and Technology （NANO-CIC）.
文摘Fluorescent silicon （Si） nanopartides （SiNPs） hold great promise for innumerable biological and biomedical applications owing to their unique optical properties and negligible toxicity. In this article, we present a new traditional Chinese medicine （TCM） molecule-assisted chemical synthetic strateg36 suitable for the production of multifunctional small-sized （diameter： - 3.7 nm） SiNPs in a facile and rapid （- 10 min） manner. Of particular significance, the resultant SiNPs simultaneously exhibited robust and stable fluorescence （photoluminescence quantum yield （PLQY）： ~ 15%）, as well as intrinsic anti-cancer efficacy with excellent selectivity toward cancer cells. Taking advantage of these unique merits, we further employed these novel fluorescent anti-cancer SiNPs （AC-SiNPs） for the fluorescence tracking and treatment of tumors, demonstrating long-term （~ 18 days） inhibition of tumor growth in tumor-bearing mice. Consequently, we believe this new TCM-assisted chemical synthetic method is highly attractive for designing silicon nanostructures featuring multiple functionalities, and we suggest these AC-SiNPs as novel promising tools for providing visual evidence of TCM-based cancer treatment.
基金Fund for Young Researchers Set by Ministry of Health of Fujian Province(No.2002-2-20)
文摘Objective: To observe the short￣term efficacy and safety of Shenqi mixture (参芪合剂, SQM) combined with microwave coagulation in treating primary hepatocellular carcinoma (HCC).Methods: Seventy-two patients with primary HCC of stage Ⅱ-Ⅲ, Karnofsky scoring ≥50 scores and predicted survival period ≥3 months were selected and randomly assigned into two groups, the treated group and the control group, 36 in each. Microwave therapy was applied to both groups by double leads, 60 W, 800 sec once a week for two weeks. To the treated group, SQM was given additionally through oral intake of 20 ml, three times a day for 1 month. The changes in tumor size, main symptoms, serum level of alpha-fetoprotein (AFP), immune function and adverse reaction were observed after treatment and the immune parameters of the patients were compared with 30 healthy persons in the normal control group. Results: (1) In the SQM treated group, after treatment 3 patients got completely remitted (CR), 24 partial remitted (PR), 4 unchanged (NC) and 5 progressively deteriorated (PD), the effective rate being 75.00%; while in the control group, 1 got CR, 19 PR, 9 NC and 7 PD, the effective rate being 55.56%. Comparison of the effective rate between the two groups showed significant difference (P<0.05). (2) AFP level decreased after treatment in both groups, but the decrement in the treated group was significantly higher than that in the control group (P<0.01). (3) After treatment, in the treated group, CD3+, CD4+, CD4+/CD8+ and NK activity were improved, Karnofsky scores increased and liver function bettered, with these improvements significantly superior to those in the control group (P<0.01). (4) The improvement in symptoms such as hepatic region pain, fever, weakness, poor appetite and jaundice in the treated group after treatment was also superior to that in the control group (P<0.01). (5)The 12-month, 18-month and 24-month survival rates were higher and the recurrence rate was lower in the treated group than those in the control group, showing significant difference (P<0.05). Conclusion: Combined therapy with SQM and microwave coagulation could not only kill the tumor and residue tumor cells to prevent recurrence, but also enhance the cellular immunity of organism. It is one of the effective therapies for patients with middle-advanced hepatocarcinoma, who have lost the chance of surgical operation. It could improve clinical symptoms, elevate the quality of life, prolong the survival period of patients, but shows no evident adverse reaction.
