The R2R3-MYB transcription factor GaPC controls petal coloration in cotton

Although a few cases of genetic epistasis in plants have been reported, the combined analysis of genetically phenotypic segregation and the related molecular mechanism remains rarely studied. Here, we have identified a gene(named GaPC) controlling petal coloration in Gossypium arboreum and following a heritable recessive epistatic genetic model. Petal coloration is controlled by a single dominant gene,GaPC. A loss-of-function mutation of GaPC leads to a recessive gene Gapc that masks the phenotype of other color genes and shows recessive epistatic interactions. Map-based cloning showed that GaPC encodes an R2R3-MYB transcription factor. A 4814-bp long terminal repeat retrotransposon insertion at the second exon led to GaPC loss of function and disabled petal coloration. GaPC controlled petal coloration by regulating the anthocyanin and flavone biosynthesis pathways. Expression of core genes in the phenylpropanoid and anthocyanin pathways was higher in colored than in white petals. Petal color was conferred by flavonoids and anthocyanins, with red and yellow petals rich in anthocyanin and flavonol glycosides, respectively. This study provides new insight on molecular mechanism of recessive epistasis,also has potential breeding value by engineering GaPC to develop colored petals or fibers for multifunctional utilization of cotton.

GATA3通过调控LIFR抑制人乳腺癌细胞的迁移能力

目的探究GATA结合蛋白3(GATA binding protein 3,GATA3)对乳腺癌细胞迁移能力的影响。方法在MCF7细胞中利用慢病毒载体介导的基因干涉技术敲低GATA3基因,使用实时定量荧光PCR(qRT-PCR)和蛋白质印迹检测GATA3和LIFR的mRNA和蛋白表达水平,Transwell实验检测MCF7细胞的迁移能力。在MCF7和T47D细胞中用染色质免疫沉淀(ChIP-qPCR)实验检测GATA3在LIFR的启动子区的结合位点。在敲低GATA3基因的MCF7细胞中回补LIFR,通过细胞划痕实验和Transwell实验检测MCF7细胞的迁移能力。结果与对照组相比,敲低GATA3基因的MCF7细胞的迁移能力增强(P_(均)<0.05)。与对照组相比,敲低GATA3基因的MCF7细胞的LIFR表达水平降低(P_(均)<0.05)。乳腺癌细胞MCF7与T47D中GATA3在LIFR的启动子区有结合(P_(均)<0.05)。在敲低GATA3基因的MCF7细胞中稳定过表达LIFR可以部分挽救GATA3基因敲低引起的细胞迁移能力的增强(P_(均)<0.05)。结论GATA3通过转录激活LIFR抑制乳腺癌细胞MCF7的迁移。

乳腺癌组织中EGFR、GATA-3、E-Cad的表达及其与预后的关系

目的探讨表皮生长因子受体(EGFR)、GATA结合蛋白3(GATA-3)、上皮性钙黏附蛋白(E-cad)在乳腺癌组织内的表达及其与患者预后的关系。方法选取89例乳腺癌患者,术中采集所有患者的癌组织与癌旁正常组织(距离病灶边缘≥3 cm),将其分别纳入观察组、对照组,统计对比2组EGFR、GATA-3、E-Cad阳性表达差异。另随访1年,统计对比不同EGFR、GATA-3、E-Cad表达患者的生存率。结果观察组EGFR、GATA-3阳性表达率高于对照组,E-Cad阳性表达率低于对照组,差异有统计学意义(P<0.05);EGFR、GATA-3、E-Cad表达与患者肿瘤的分化程度、淋巴结转移、临床分期有关,差异有统计学意义(P<0.05)。EGFR、GATA-3阳性表达患者的生存率低于阴性表达患者,E-Cad阴性表达患者的生存率低于阳性表达患者,差异有统计学意义(P<0.05)。结论EGFR、GATA-3在乳腺癌组织内呈高表达,E-Cad呈低表达,三者表达与乳腺癌患者的生存率具有紧密联系。

Chlorophyllase is transcriptionally regulated by CsMYB308/CsDOF3 in young leaves of tea plant

Chlorophyll contributes to tea coloration, which is an important factor in tea quality. Chlorophyll metabolism is induced by light, but the transcriptional regulation responsible for light-induced chlorophyll metabolism is largely unknown in tea leaves. Here, we characterized a chlorophyllase1 gene CsCLH1 from young tea leaves and showed it is essential for chlorophyll metabolism, using transient overexpression and silencing in tea leaves and ectopic overexpression in Arabidopsis. CsCLH1 was significantly induced by high light. The DOF protein CsDOF3, an upstream direct regulator of CsCLH1, was also identified. Acting as a nuclear-localized transcriptional factor, CsDOF3 responded for light and repressed CsCLH1 transcription and increased chlorophyll content by directly binding to the AAAG cis-element in the CsCLH1 promoter. CsDOF3was able to physically interact with the R2R3-MYB transcription factor CsMYB308 and interfere with transcriptional activity of CsCLH1. In addition, CsMYB308 binds to the CsCLH1 promoter to enhance CsCLH1 expression and decrease chlorophyll content. CsMYB308 and CsDOF3 act as an antagonistic complex to regulate CsCLH1 transcription and chlorophyll in young leaves. Collectively, the study adds to the understanding of the transcriptional regulation of chlorophyll in tea leaves in response to light and provides a basis for improving the appearance of tea.

Basic transcription factor 3 is involved in gastric cancer development and progression

AIM: To further analyse cancer involvement of basic transcription factor 3 (BTF3) after detection of its upregulation in gastric tumor samples. METHODS: BTF3 transcription rates in human gastric tumor tissue samples (n = 20) and adjacent normal tissue (n = 18) specimens as well as in the gastric cancer cell lines AGS, SGC-7901, MKN-28, MKN-45 and MGC803 were analyzed via quantitative real-time polymerase chain reaction. The effect of stable BTF3 silencing via infection with a small interfering RNA (siRNA)-BTF3 expressing lentivirus on SGC-7901 cells was measured via Western blotting analysis, proliferation assays, cell cycle and apoptosis profiling by flow cytometry as well as colony forming assays with a Cellomic Assay System. RESULTS: A significant higher expression of BTF3 mRNA was detected in tumors compared to normal gastric tissues (P < 0.01), especially in section tissues from female patients compared to male patients, and all tested gastric cancer cell lines expressed high levels of BTF3. From days 1 to 5, the relative proliferation rates of stable BTF3-siRNA transfected SGC7901 cells were 82%, 70%, 57%, 49% and 44% compared to the control, while the percentage of cells arrested in the G 1 phase was significantly decreased (P = 0.000) and the percentages of cells in the S (P = 0.031) and G 2 /M (P = 0.027) phases were significantly increased. In addition, the colony forming tendency was significantly decreased (P = 0.014) and the apoptosis rate increased from 5.73% to 8.59% (P = 0.014) after BTF3 was silenced in SGC7901 cells. CONCLUSION: BTF3 expression is associated with enhanced cell proliferation, reduced cell cycle regulation and apoptosis and its silencing decreased colony forming and proliferation of gastric cancer cells.

Function of GATA transcription factors in hydroxyurea-induced HEL cells

HEL cells, a human erythroleukemia cell line, mainly express the fetal (r)globin gene and trace amount of the embryonic (E)globin gene, but not adult (B) globin gene. Here we show that hydroxyurea (HU) can induce HEL cells to express adult (B) globin gene and lead these cells to terminal differentiation. Results showed in Gel mobility shift assays that GATA factors could specifically bind to the regulatory elements of human B- globin gene, including the proximal regulatory element (the B- promoter) and the distal regulatory elements (the DNase I hypersensitive sites in the LCR, HS2-HS4 core sequences). However, the DNA binding patterns of GATA factors were quite different between HU-induced and uninduced HEL cells. Western-blot analysis of nuclear extracts from both the uninduced and HU- induced HEL cells revealed that the level of GATA-2 transcription factor decreased, whereas the level of GATA-1 transcription factor increased following the time of hydroxyurea induction. Furthermore, using RT-PCR analysis the expression of human B-globin gene in HU-induced HEL cells could be blocked again when HEL cells were incubated in the presence of antisense oligonucleotides for hGATA-1, suggesting that the upregulation of hGATA-1 transcription factor might be critical for the expression of human β- globin gene in HU-induced HEL cells.

Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato

BRI1-EMS-SUPPRESSOR 1(BES1)transcription factor is closely associated with the brassinosteroid(BR)signaling pathway and plays an important role in plant growth and development.SLB3 is a member of BES1 transcription factor family and its expression was previously shown to increase significantly in tomato seedlings under drought stress.In the present study,we used virus-induced gene silencing(VIGS)technology to downregulate SLB3 expression to reveal the function of the SLB3 gene under drought stress further.The downregulated expression of SLB3 weakened the drought tolerance of the plants appeared earlier wilting and higher accumulation of H2 O2 and O2^–·,decreased superoxide dismutase(SOD)activity,and increased proline(PRO)and malondialdehyde(MDA)contents and peroxidase(POD)activity.Quantitative real-time PCR(qRT-PCR)analysis of BR-related genes revealed that the expression of SlCPD,SlDWARF and BIN2-related genes was significantly upregulated in SLB3-silenced seedlings under drought stress,but that the expression of TCH4-related genes was downregulated.These results showed that silencing the SLB3 gene reduced the drought resistance of tomato plants and had an impact on the BR signaling transduction which may be probably responsible for the variation in drought resistance of the tomato plants.

Genome-wide analysis of the B3 transcription factors reveals that RcABI3/VP1 subfamily plays important roles in seed development and oil storage in castor bean(Ricinus communis)

The B3 transcription factors(TFs)in plants play vital roles in numerous biological processes.Although B3 genes have been broadly identified in many plants,little is known about their potential functions in mediating seed development and material accumulation.Castor bean(Ricinus communis)is a non-edible oilseed crop considered an ideal model system for seed biology research.Here,we identified a total of 61 B3 genes in the castor bean genome,which can be classified into five subfamilies,including ABI3/VP1,HSI,ARF,RAV and REM.The expression profiles revealed that RcABI3/VP1 subfamily genes are significantly up-regulated in the middle and later stages of seed development,indicating that these genes may be associated with the accumulation of storage oils.Furthermore,through yeast one-hybrid and tobacco transient expression assays,we detected that ABI3/VP1 subfamily member RcLEC2 directly regulates the transcription of RcOleosin2,which encodes an oil-body structural protein.This finding suggests that RcLEC2,as a seed-specific TF,may be involved in the regulation of storage materials accumulation.This study provides novel insights into the potential roles and molecular basis of B3 family proteins in seed development and material accumulation.

<i>Trapa japonica</i>Flerov Extract Attenuates Lipid Accumulation through Downregulation of Adipogenic Transcription Factors in 3T3-L1 Cells

Obesity is a major human health problem associated with various diseases, including cardiac injury and type 2 diabetes. Trapa japonica Flerov (TJF) has been used in traditional oriental medicine to treat diabetes. In this study, we evaluated the inhibitory effect of and the mechanism underlying the effect of TJF extract on adipogenesis in 3T3-L1 cells. The effects of TJF extract on cell viability were analyzed using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, and the anti-adipogenic effect was measured by oil red O staining. The expression of peroxisomal proliferator activated receptor (PPAR)γ, CCAAT/enhancer-binding protein-α (C/EBP)α, adenosine monophosphate-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), adiponectin, and fatty acid binding protein (FABP)4 involved in adipogenesis was determined by western blot analysis. TJF extract effectively inhibited lipid accumulation and the expression of PPARγ and C/EBPα in 3T3-L1 cells. TJF also increased the phosphorylation of AMPK and ACC, and decreased the expression of adiponectin and FABP4. These results indicate that TJF extract exerts its anti-obesity effect through the downregulation of adipogenic transcription factors and adipogenic marker genes.

Increased Electrophoretic Mobility of Long-Type GATA-6 Transcription Factor upon Substitution of Its PEST Sequence

The transcriptional factor GATA-6 gene produces two translational isoforms from a single mRNA through ribosomal leaky scanning. L-type GATA-6 has an extension of 146 amino acid residues at its amino terminus. In the extension, there is a unique PEST sequence (Glu31-Cys46), which is composed of an amino terminal Pro-rich segment and a carboxyl terminal Ser-cluster. Substitution of either half of the PEST sequence with Ala residues by cassette mutagenesis reduced the apparent molecular size of L-type GATA-6 on SDS-polyacrylamide gel-electrophoresis. However, the effect of substitution of the Pro-rich segment was much more significant;the mobility increase of the Pro-rich segment on the gel was 13% while that of the Ser-cluster was 8%. Substitution of each amino acid residue demonstrated that the effect of Pro substitution is greater than that of the Ser and Thr residues. Such increased mobility of L-type GATA-6 in the presence of a detergent may apparently correlate with the decrease in transcription activity in vivo as determined by means of luciferase reporter gene assay. The activity of ΔAla (with Ala residues instead of the PEST sequence) was reduced to one fifth of that of ΔA (with the PEST sequence). These results suggest that the PEST sequence of L-type GATA-6 does not function as a constitutive protein degradation signal, but rather plays structural and functional roles in the activation of gene expression on the GATA responsive promoter.

Comparative transcriptomic analysis of Rosa sterilis inflorescence branches with different trichome types reveals an R3-MYB transcription factor that negatively regulates trichome formation

Rosa sterilis S.D.Shi is an important economic tree in China that produces fruits with high nutritional and medicinal value.Many of R.sterills’organs are covered with different types of trichomes or prickles that directly affect fruit appearance and plant management.This study used RNA sequencing technology to analyze the transcriptomes of two parts of the inflorescence branch,namely inflorescence stems with flagellated trichomes and pedicels with both flagellated and glandular trichomes.Comparative transcriptomic analysis showed that many transcription factors(TFs)are potentially involved in the formation and development of trichomes.The accumulation of RsETC1,a TF of the R3-MYB family,was significantly higher in inflorescence stems than in pedicels;quantitative reverse transcription PCR(qRTPCR)verified that its expression was significantly higher in inflorescence stems than in pedicels during the first three development stages,indicating its inhibitory action on the initiation of glandular trichomes in R.sterilis.The mRNA level of RsETC1 accumulated to significantly higher levels in trichomeless tissues than in tissues with trichromes,suggesting that this gene may inhibit the formation of trichomes in R.sterilis.Over-expression of RsETC1 in Arabidopsis resulted in glabrous phenotypes,and the expression of trichome-related endogenous genes,except for TTG1,was markedly reduced.In addition,the contents of the phytohormones jasmonic acid(JA),gibberellin A3(GA_(3)),and cytokinins(CKs)in pedicels were significantly higher than those in inflorescence stems,and the expression patterns of the genes related to hormone biosynthesis and signal transduction presented consistent responses,suggesting that the transduction of these hormones might be crucial for trichome initiation and development.These data provide a new perspective for revealing the molecular mechanism of trichome formation in R.sterilis.

Ectopic expression of VvFUS3,B3-domain transcription factor,in tomato influences seed development via affecting endoreduplication and hormones

FUSCA3(FUS3)is a member of B3-domain transcription factor family and master regulator of seed development.It has potential roles in hormone biosynthesis and signaling pathways and therefore plays diverse roles in plant life cycle,especially in seed germination,dormancy,embryo formation,seed and fruit development,and maturation.However,there is limited information about its functions in seed and fruit development of grapevine.In this study,we expressed VvFUS3 in tomato for its functional characterization.Overexpression of VvFUS3 in tomato led to a reduction in seed number and seed weight without affecting the fruit size.Histological analysis found that both cell expansion and cell division in transgenic seed and fruit pericarp have been affected.However,there were no obvious differences in pollen size,shape,and viability,suggesting that VvFUS3 affects seed development but not the pollen grains.Moreover,the expression of several genes with presumed roles in seed development and hormone signaling pathways was also influenced by VvFUS3.These results suggest that VvFUS3 is involved in hormonal signaling pathways that regulate seed number and size.In conclusion,our study provides novel preliminary information about the pivotal roles of VvFUS3 in seed and fruit development and these findings can potentially serve as a reference for molecular breeding of seedless grapes.

妊娠高血压胎盘病理检查及VEGF、GATA-3异常表达的变化及其临床意义

目的探讨妊娠高血压妇女胎盘组织的病理学改变特征、组织中血管内皮生长因子(VEGF)、GATA蛋白3(GATA-3)表达异常及其临床意义。方法回顾性选取在淮北市妇幼保健院接受产前检查且分娩的妊娠高血压孕妇100例(病例组)、选取同期正常妊娠分娩的孕妇100例作为对照组。病例组根据病情分为:妊娠期高血压22例,子痫前期轻度14例,子痫前期重度64例。对比各组胎盘病理学特征、胎盘组织中的VEGF、GATA-3蛋白的表达情况,并采用受试者工作曲线(ROC)分析VEGF、GATA-3蛋白表达与不良分娩结局的关系。结果病例组的胎盘绒毛间隙纤维素沉积、胎盘绒毛间质纤维化、细胞滋养细胞增生、绒毛血管减少检出率为37.00%、47.00%、62.00%、28.00%,显著高于对照组(9.00%、4.00%、14.00%、3.00%),差异均有统计学意义(P<0.05)。病例组的胎盘组织的VEGF、GATA-3蛋白阳性表达率为34.00%、21.00%,均低于对照组(65.00%、57.00%),差异均有统计学意义(P<0.05)。子痫前期重度(68.75%、84.38%、68.75%、57.81%)、子痫前期轻度(50.00%、71.43%、64.29%、35.71%)患者的胎盘绒毛间隙纤维素沉积、胎盘绒毛间质纤维化、绒毛血管减少检出率显著高于妊娠期高血压组(18.18%、22.73%、59.09%、13.64%),差异均有统计学意义(P<0.05)。子痫前期重度(10.94%、4.69%)、子痫前期轻度(21.43%、7.14%)患者的胎盘组织的VEGF、GATA-3蛋白阳性表达率均低于妊娠高血压组(50.00%、31.81%),差异均有统计学意义(P<0.05)。不良分娩结局组胎盘组织中的VEGF、GATA-3阳性表达率为14.29%、4.76%,显著低于良好组(39.24%、25.32%),差异均有统计学意义(P<0.05);VEGF、GATA-3蛋白预测妊娠高血压孕妇不良分娩结局的ROC曲线下面积值分别为0.732、0.850。结论妊娠高血压妇女胎盘组织病理学改变特征明显,VEGF、GATA-3阳性表达率显著降低,并且与患者病情程度、不良分娩结局有关。

The Transcription Factors GATA-1 and GATA-4 Have Opposite Effects on DNA Expression Driven by an Amh Promoter

An Amh promoter driving expression of a reporter gene (d2EGFP) has been used to analyze the role of two specific promoter transcription factor binding elements. In addition a downstream (3’) enhancer (DE) was also investigated. The transcription factors GATA-1 and GATA-4 had opposite effects, the former being incremental and the latter decremental. The quantitative balance between these two factors may provide a degree of control over the level of gene expression.

Correlation of Runt-related transcription factor gene 3 expression in osteosarcoma tissue with cell proliferation and angiogenesis

Objective:To study the correlation of Runt-related transcription factor gene 3 (RUNX3) expression in osteosarcoma tissue with cell proliferation and angiogenesis.Methods: A total of 80 patients with osteosarcoma who were treated in our hospital between February 2014 and February 2017 were collected, and the RUNX3 expression in osteosarcoma tissue and adjacent tissue were detected. According to the RUNX3 expression in tumor tissue, the patients were further divided into high RUNX3 expression group and low RUNX3 expression group, and the proliferation gene and angiogenesis gene expression were compared.Results:RUNX3, KISS-1 and RanBP9 mRNA expression in osteosarcoma tissue were significantly lower than those in adjacent tissue while VCP, Six1, S100A6, IF-1α, MMP-14, bFGF and Ang-2 mRNA expression were significantly higher than those in adjacent tissue;KISS-1 and RanBP9 mRNA expression in osteosarcoma tissue of high RUNX3 expression group were significantly higher than those of low RUNX3 expression group while VCP, Six1, S100A6, IF-1 , MMP-14, bFGF and Ang-2 mRNA expression were significantly lower than those of low RUNX3 expression group.Conclusions:The desease of RUNX3 expression in osteosarcoma tissue is one of the direct causes of increased tumor proliferation activity and strong angiogenesis.

Important Roles of Transcription Factors in Regulating Seed Oil Biosynthesis to Increase Plant Storage Lipid Content

In this article, the biosynthetic pathways of storage oil accumulation in oilseed plants were briefly introduced, and the transcription factors, such as B3 do- main supeffamily genes, lecl gene, wril gene etc., and their important role in oil accumulation regulation was mainly elucidated. Overexpession of transcription factors as feasible ways of genetic manipulation to increase oJl content in oilseed crops are promising in a long-term perspective.

活动性肺结核患者血清ATG3和FOXO3水平变化对患者病情进展及预后评估的相关性分析

目的分析活动性肺结核患者血清中自噬相关基因3(ATG3)和叉头转录因子O亚型3(FOXO3)表达水平变化以及与患者病情进展及预后的关系。方法以2019年9月~2022年9月于本院就诊的91例活动性肺结核患者(观察组)为研究对象,另选取同时期于本院体检的91例健康体检者作为对照组;酶联免疫吸附法检测血清中ATG3和FOXO3表达水平;利用Spearman相关分析活动性肺结核患者血清中ATG3和FOXO3表达水平与患者病情严重程度之间的相关性;采用Logistic回归分析影响活动性肺结核患者预后的因素;采用ROC曲线分析血清中ATG3和FOXO3表达水平对活动性肺结核患者预后评估的价值。结果与对照组相比,观察组血清中ATG3和FOXO3表达水平显著下降(P<0.05);与轻症组相比,重症组患者血清中ATG3和FOXO3表达水平显著下降(P<0.05);与预后不良组相比,预后良好组患者血清中ATG3和FOXO3表达水平显著升高(P<0.05);预后良好组与预后不良组患者ESR、PCT、CRP、TNF-α、INF-γ和IL-2相比差异具有统计学意义(P<0.05);Spearman相关分析显示活动性肺结核患者血清中ATG3和FOXO3表达水平与患者病情严重程度呈负相关(P<0.05);Logistic回归分析结果显示,ESR、CRP、ATG3和FOXO3为影响活动性肺结核患者预后不良的因素(P<0.05);ROC曲线分析显示,血清中ATG3和FOXO3表达水平联合预测活动性肺结核患者预后较ATG3和FOXO3单一指标预测效果更优(P<0.05)。结论活动性肺结核患者血清中ATG3和FOXO3表达水平显著下降,且与活动性肺结核病情严重程度相关,二者联合对活动性肺结核患者预后具有较高的评估价值。

颅脑损伤术后颅脑感染血清NLRP3、SAA及NFκB的临床意义

目的探讨血清寡聚化结构域样受体蛋白3(NLRP3)、淀粉样蛋白A(SAA)及核转录因子(NFκB)在颅脑损伤术后颅脑感染中的诊断价值。方法选择2020年6月至2022年6月江苏省如皋市人民医院接诊的70例颅脑损伤患者为研究对象,根据感染情况将感染者设为感染组(n=19),未感染者设为对照组(n=51),分析血清NLRP3、SAA及NFκB在颅脑损伤术后颅脑感染中的诊断价值。结果感染组患者血清NLRP3、SAA及NFκB水平显著高于对照组,差异有统计学意义(P<0.05);NLRP3、SAA及NFκB水平:轻度感染<中度感染<重度感染,差异有统计学意义(P<0.05);预后不良组患者血清NLRP3、SAA及NFκB水平显著高于预后良好组,差异有统计学意义(P<0.05);ROC结果显示,血清NLRP3预测颅脑损伤术后颅脑感染的AUC为0.634,灵敏度为63.43%,特异度为67.40%,截断值为114.02 pg/mL;血清SAA预测颅脑损伤术后颅脑感染的AUC为0.715,灵敏度73.50%,特异度为69.00%,截断值为30.99 mg/L;血清NFκB预测颅脑损伤术后颅脑感染的AUC为0.914,灵敏度为81.40%,特异度为70.00%,截断值为38.27μg/mL,联合检测较单独检测特异度、准确度更高(P<0.05)。结论血清NLRP3、SAA及NFκB在颅脑损伤术后颅脑感染患者中均异常高表达,且三指标联合检测颅脑损伤术后颅脑感染诊断效能更高,临床应用价值高。

三阴性乳腺癌患者癌组织中RUNX3及JAM-A表达变化及与疾病预后的相关性分析

目的探讨三阴性乳腺癌患者癌组织中Runt相关转录因子3(RUNX3)及连接黏附分子A(JAMA)表达变化及与疾病预后的相关性。方法选取2016年1月至2018年3月在新疆维吾尔自治区人民医院确诊的三阴性乳腺癌患者60例作为研究对象,收集患者乳腺癌组织及癌旁组织,通过实时荧光定量PCR及酶联免疫吸附试验检测并对比癌组织、癌旁组织中RUNX3、JAM-A转录水平及蛋白水平。对比不同临床病理特征三阴性乳腺癌患者癌组织中RUNX3及JAM-A转录水平及蛋白水平。采用Spearman相关性分析三阴性乳腺癌患者癌组织中RUNX3、JAM-A表达与患者不良预后的相关性。结果三阴性乳腺癌患者癌组织中RUNX3转录水平显著低于癌旁组织,JAM-A转录水平显著高于癌旁组织,差异有统计学意义(P<0.05)。三阴性乳腺癌患者癌组织中RUNX3蛋白水平显著低于癌旁组织,JAM-A蛋白水平显著高于癌旁组织,差异有统计学意义(P<0.05)。不同肿瘤最大径、TNM分期及淋巴结转移情况的三阴性乳腺癌患者癌组织中RUNX3及JAM-A转录水平比较,差异有统计学意义(P<0.05)。不同肿瘤最大径、TNM分期及淋巴结转移情况的三阴性乳腺癌患者癌组织中RUNX3及JAM-A蛋白水平比较,差异有统计学意义(P<0.05)。Spearman相关性分析表明,三阴性乳腺癌患者癌组织中RUNX3转录水平及蛋白水平与不良预后呈负相关,JAM-A转录水平及蛋白水平与不良预后呈正相关(P<0.05)。结论三阴性乳腺癌患者癌组织中RUNX3表达显著下调、JAM-A表达显著上调,且与患者预后均存在一定相关性,RUNX3及JAM-A可能是三阴性乳腺癌发生、发展过程中的重要调控因子。

YTH结构域家族蛋白2和叉头蛋白转录因子3在肺腺癌中的表达关系研究

目的 分析YTH结构域家族蛋白2(YTHDF2)及叉头蛋白转录因子3(FOXO3)在肺腺癌病人中的表达及与预后的关系。方法 收集2020年1月至2021年5月在郑州大学第一附属医院行手术治疗的肺腺癌病人癌组织及对应的癌旁组织(距离癌组织5 cm以上)80对,收集病人临床病理资料;利用癌症基因组图谱(TCGA)数据库查询YTHDF2、FOXO3基因在肺腺癌中的表达水平;采用免疫组织化学法检测YTHDF2、FOXO3蛋白在肺腺癌组织中的表达情况;分析YTHDF2、FOXO3蛋白水平与肺腺癌病人临床病理资料的关系;分析肺腺癌中YTHDF2与FOXO3基因表达水平的相关性;对病人进行为期3年的随访,分析病人3年累积生存率。结果 YTHDF2、FOXO3蛋白在肺腺癌组织中的高表达率分别为34.00%、26.00%,明显低于癌旁正常组织中79.48%、61.54%(P<0.05)。YTHDF2蛋白表达情况与肺腺癌病人年龄、淋巴结转移和分化程度相关(P<0.05);与TNM分期、性别无关(P>0.05);FOXO3蛋白表达情况与肺腺癌病人性别和分化程度相关(P<0.05);肺腺癌组织中YTHDF2蛋白高表达组和低表达组病人3年累积生存率分别为53.30%、14.00%,经log-rank比较,差异有统计学意义(P<0.05);FOXO3蛋白高表达组和低表达组病人3年累积生存率分别为64.50%、12.20%,经Log-Rank比较,差异有统计学意义(P<0.05)。结论 YTHDF2、FOXO3在肺腺癌组织中均低表达,与病人肿瘤分化程度及3年累积生存率有关,有望成为评估肺腺癌病人预后的生物标志物。