In this paper, a two level finite difference scheme of Crank-Nicholson type is constructed and used to numerically investigate nonlinear temperature distribution in biological tissues described by bioheat transfer equ...In this paper, a two level finite difference scheme of Crank-Nicholson type is constructed and used to numerically investigate nonlinear temperature distribution in biological tissues described by bioheat transfer equation of Pennes’ type. For the equation under consideration, the thermal conductivity is either depth-dependent or tem-perature-dependent, while blood perfusion is temperature-dependent. In both cases of depth- dependent and temperature-dependent thermal conductivity, it is shown that blood perfusion decreases the temperature of the living tissue. Our numerical simulations show that neither the localization nor the magnitude of peak tempera-ture is affected by surface temperature;however, the width of peak temperature increases with surface temperature.展开更多
Two charge-transfer complexes of 2a and 2b of Keggin type were synthesized and characterized by elemental analysis, IR spectra, UV spectra, XRD, TG-DSC; and were compared with Hquin-PW12. The primary structure of the...Two charge-transfer complexes of 2a and 2b of Keggin type were synthesized and characterized by elemental analysis, IR spectra, UV spectra, XRD, TG-DSC; and were compared with Hquin-PW12. The primary structure of the heteropolyanions had not been changed after the formation of the charge transfer complexes.展开更多
The population transfer in a ladder-type atomic system driven by linearly polarized sech-shape femtosecond laser pulses is investigated by numerically solving Schr6dinger equation without including the rotating wave a...The population transfer in a ladder-type atomic system driven by linearly polarized sech-shape femtosecond laser pulses is investigated by numerically solving Schr6dinger equation without including the rotating wave approximation (RWA). It is shown that population transfer is mainly determined by the Rabi frequency (strength) of the driving laser field and the chirp rate, and that the ratio of the dipole moments and the pulse width also have a prominent effect on the population transfer. By choosing appropriate values of the above parameters, complete population transfer can be realized.展开更多
Type-A spermatogonia first appear at between 3-7 d postnatally in mice and are the only immortalized diploid cells that reproduce in adulthood in these animals. In our current study, we explored the feasibility of pro...Type-A spermatogonia first appear at between 3-7 d postnatally in mice and are the only immortalized diploid cells that reproduce in adulthood in these animals. In our current study, we explored the feasibility of producing stable transgenic mice using these cells. Enhanced pEGFP-N1 plasmids were suspended in ExGen500 transfection reagent and injected at different angles into the testes of 7-d-old male ICR mice. The resulting type-A spermatogonia-mediated gene transfer (TASMGT) mice were then mated with normal females at different stages of sexual maturity (6, 12, and 24 wk). The integration and expression of the introduced EGFP gene was evaluated in the F1 transgenic offspring by PCR and Southern blotting analysis. The foreign gene integration rates for a low-dose group (15 μL gene suspension injected into each testis) and a high-dose group (30 μL suspensions injected) at the three stages of female sexual maturity tested were 11.76% (2/17), 14.29% (3/21), and 11.11% (2/18), and 5% (1/20), 5.56% (1/18), and 0 (0/17), respectively. The average integration rates for these two dose groups were 12.5% (7/56) and 3.64% (2/55), respectively, which was a significant difference (P0.05). Semi-quantitative RT-PCR analysis further showed that the introduced GFP gene was expressed in 3/9 integration mice. In addition, GFP expression was observed in the sperm cells from the TASMGT mice, and also in the embryos and F2 pups from the F1 generation transgenic mice. Hence, although the foreign gene integration rate for TASMGT is not high and the transgenic offspring show as yet unexplained defects, our results indicate that this method is a potentially feasible and reproducible new approach to creating transgenic mice.展开更多
Two species of radishes, Raphanus sativus and Raphanus raphanistrum, were grown in the laboratory in the same substrate consisting of a smectite-type clay, which was watered at the beginning of the experience with 50 ...Two species of radishes, Raphanus sativus and Raphanus raphanistrum, were grown in the laboratory in the same substrate consisting of a smectite-type clay, which was watered at the beginning of the experience with 50 ml of a solution containing either none or 1000, 2000 or 4000 mg/L of AgNO3, respectively. Occurrence of the toxic metal in the substrate outlines higher element uptakes by the cultivated species Raphanus sativus than by the wild species Raphanus raphanistrum, except for the highest degree of Ag pollution. After a one-month growth in the Ag-polluted substrate, Raphanus sativus was depleted in most of the major, trace and rare-earth elements, except for Al, Fe, Th, Ag and U that increased in the radishes from substrate polluted by 2000 mg/L of AgNO3, and Sr, Co, Ni, U and Ag that increased in the radishes from substrate polluted by 4000 mg/L of AgNO3. Raphanus raphanistrum was enriched in all elements except Si, Na, Rb and K in the polluted substrate. The uptake was monitored by a cation-exchange process in the rhy-zosphere between mineral particles and the watering solution in the presence of various enzymes with specific activities that induced a variable uptake with the REEs being even fractionated. These activities most probably depend on combined factors, such as the plant species, and the chemical and physical properties of the substrate. The results obtained here reveal also that accumulation of nutrient elements and others in the plants is not uniform at a given Ag pollution of the substrate and therefore at a given Ag contamination in the same plant species.展开更多
变压器热点温度作为运行经济性、安全性的关键指标,是设备在线监测及状态评估中的重点。该文对变压器内部散热进行研究,着眼于饼式绕组及其油道结构,基于能量流向建立绕组温度变化的物理模型。基于这一模型,在一台内置分布式传感光纤的1...变压器热点温度作为运行经济性、安全性的关键指标,是设备在线监测及状态评估中的重点。该文对变压器内部散热进行研究,着眼于饼式绕组及其油道结构,基于能量流向建立绕组温度变化的物理模型。基于这一模型,在一台内置分布式传感光纤的110kV三相ONAN变压器上开展试验研究,使用分布式光纤测温(distributed temperature sensing,DTS)技术对运行状况下的绕组整体温度分布进行实时监测,分析绕组在ONAN冷却方式下的散热状况。在变压器启动初期,绕组各处散热量较低,温升速率较快。约2 h后,各饼散热量基本与损耗相一致,散热率可达98%以上,因此将这一阶段称为准稳态。准稳态阶段,绕组整体散热率基本一致。负载变化前期不同位置散热量的差异是温度梯度形成的主要原因。基于DTS手段及散热器进出口处油温,提出绕组每饼平均对流换热系数的计算方法,基于无量纲数建立绕组内外表面局部对流换热系数的计算方法,对不同位置、负载率下两种对流换热系数的变化规律进行分析获得了绕组运行过程中对流换热系数分布规律及变化趋势。展开更多
文摘In this paper, a two level finite difference scheme of Crank-Nicholson type is constructed and used to numerically investigate nonlinear temperature distribution in biological tissues described by bioheat transfer equation of Pennes’ type. For the equation under consideration, the thermal conductivity is either depth-dependent or tem-perature-dependent, while blood perfusion is temperature-dependent. In both cases of depth- dependent and temperature-dependent thermal conductivity, it is shown that blood perfusion decreases the temperature of the living tissue. Our numerical simulations show that neither the localization nor the magnitude of peak tempera-ture is affected by surface temperature;however, the width of peak temperature increases with surface temperature.
文摘Two charge-transfer complexes of 2a and 2b of Keggin type were synthesized and characterized by elemental analysis, IR spectra, UV spectra, XRD, TG-DSC; and were compared with Hquin-PW12. The primary structure of the heteropolyanions had not been changed after the formation of the charge transfer complexes.
基金supported by National Basic Research Program of China (Grant No 2006CB806003)the Natural Science Foundation of Shandong Province,China (Grant No Y2006A21)+1 种基金the National Natural Science Foundation of China (Grant No 10675076)State Key Laboratory of High Field Laser Physics,Shanghai Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,China
文摘The population transfer in a ladder-type atomic system driven by linearly polarized sech-shape femtosecond laser pulses is investigated by numerically solving Schr6dinger equation without including the rotating wave approximation (RWA). It is shown that population transfer is mainly determined by the Rabi frequency (strength) of the driving laser field and the chirp rate, and that the ratio of the dipole moments and the pulse width also have a prominent effect on the population transfer. By choosing appropriate values of the above parameters, complete population transfer can be realized.
基金supported by the National Transgenic Breeding Project (2008ZX08010-004)the National Natural Science Foundation of China (30830080)+1 种基金the National 973 Program of China (G2006CB102105,2009CB941604)the National 863 Program of China (20060110Z1039, 2008AA10Z143)
文摘Type-A spermatogonia first appear at between 3-7 d postnatally in mice and are the only immortalized diploid cells that reproduce in adulthood in these animals. In our current study, we explored the feasibility of producing stable transgenic mice using these cells. Enhanced pEGFP-N1 plasmids were suspended in ExGen500 transfection reagent and injected at different angles into the testes of 7-d-old male ICR mice. The resulting type-A spermatogonia-mediated gene transfer (TASMGT) mice were then mated with normal females at different stages of sexual maturity (6, 12, and 24 wk). The integration and expression of the introduced EGFP gene was evaluated in the F1 transgenic offspring by PCR and Southern blotting analysis. The foreign gene integration rates for a low-dose group (15 μL gene suspension injected into each testis) and a high-dose group (30 μL suspensions injected) at the three stages of female sexual maturity tested were 11.76% (2/17), 14.29% (3/21), and 11.11% (2/18), and 5% (1/20), 5.56% (1/18), and 0 (0/17), respectively. The average integration rates for these two dose groups were 12.5% (7/56) and 3.64% (2/55), respectively, which was a significant difference (P0.05). Semi-quantitative RT-PCR analysis further showed that the introduced GFP gene was expressed in 3/9 integration mice. In addition, GFP expression was observed in the sperm cells from the TASMGT mice, and also in the embryos and F2 pups from the F1 generation transgenic mice. Hence, although the foreign gene integration rate for TASMGT is not high and the transgenic offspring show as yet unexplained defects, our results indicate that this method is a potentially feasible and reproducible new approach to creating transgenic mice.
文摘Two species of radishes, Raphanus sativus and Raphanus raphanistrum, were grown in the laboratory in the same substrate consisting of a smectite-type clay, which was watered at the beginning of the experience with 50 ml of a solution containing either none or 1000, 2000 or 4000 mg/L of AgNO3, respectively. Occurrence of the toxic metal in the substrate outlines higher element uptakes by the cultivated species Raphanus sativus than by the wild species Raphanus raphanistrum, except for the highest degree of Ag pollution. After a one-month growth in the Ag-polluted substrate, Raphanus sativus was depleted in most of the major, trace and rare-earth elements, except for Al, Fe, Th, Ag and U that increased in the radishes from substrate polluted by 2000 mg/L of AgNO3, and Sr, Co, Ni, U and Ag that increased in the radishes from substrate polluted by 4000 mg/L of AgNO3. Raphanus raphanistrum was enriched in all elements except Si, Na, Rb and K in the polluted substrate. The uptake was monitored by a cation-exchange process in the rhy-zosphere between mineral particles and the watering solution in the presence of various enzymes with specific activities that induced a variable uptake with the REEs being even fractionated. These activities most probably depend on combined factors, such as the plant species, and the chemical and physical properties of the substrate. The results obtained here reveal also that accumulation of nutrient elements and others in the plants is not uniform at a given Ag pollution of the substrate and therefore at a given Ag contamination in the same plant species.
文摘变压器热点温度作为运行经济性、安全性的关键指标,是设备在线监测及状态评估中的重点。该文对变压器内部散热进行研究,着眼于饼式绕组及其油道结构,基于能量流向建立绕组温度变化的物理模型。基于这一模型,在一台内置分布式传感光纤的110kV三相ONAN变压器上开展试验研究,使用分布式光纤测温(distributed temperature sensing,DTS)技术对运行状况下的绕组整体温度分布进行实时监测,分析绕组在ONAN冷却方式下的散热状况。在变压器启动初期,绕组各处散热量较低,温升速率较快。约2 h后,各饼散热量基本与损耗相一致,散热率可达98%以上,因此将这一阶段称为准稳态。准稳态阶段,绕组整体散热率基本一致。负载变化前期不同位置散热量的差异是温度梯度形成的主要原因。基于DTS手段及散热器进出口处油温,提出绕组每饼平均对流换热系数的计算方法,基于无量纲数建立绕组内外表面局部对流换热系数的计算方法,对不同位置、负载率下两种对流换热系数的变化规律进行分析获得了绕组运行过程中对流换热系数分布规律及变化趋势。