AIM： To identify and characterize drosophila mothers against decapentaplegic （SMAD）3-dependent changes in immune cell populations following infection with He- Iicobacter hepaticus （H. hepaticus）. METHODS： SMAD3/...AIM： To identify and characterize drosophila mothers against decapentaplegic （SMAD）3-dependent changes in immune cell populations following infection with He- Iicobacter hepaticus （H. hepaticus）. METHODS： SMAD3/ （n = L9） and colitis-resistant SMAD3＋/ （n = 24） mice （8-10 wk of age） were in- fected with/-/, hepaticus and changes in immune cell populations [T lymphocytes, natural killer （NK） cells, T regulatory cells] were measured in the spleen and mesenteric lymph nodes （MsLNs） at 0 d, 3 d, 7 d and 28 d post-infection using flow cytometry. Genotype-dependent changes in T lymphocytes and granzyme B＋ cells were also assessed after 28 d in proximal colon tissue using immunohistochemistry. RESULTS： As previously observed, SMAD3＋, but not SMAD3＋/- mice, developed colitis, peaking at 4 wk post-infection. No significant changes in T cell subsets were observed in the spleen or in the MsLNs between genotypes at any time point. However, CD4＋ and CD8＋/ CD62L＋＋ cells, an effector T lymphocyte population, as well as NK cells （NKp46/DX5＋） were significantly higher in the MsLNs of SMAD3/ mice at 7 d and 28 d post-in- fection. In the colon, a higher number of CD3＋ cells were present in SMAD3＋ compared to SMAD3＋/- mice at base- line, which did not significantly change during infection. However, the number of granzyme B＋ cells, a marker of cytolytic lymphoo/tes, significantly increased in SMAD3＋ mice 28 d post-infection compared to both SMAD3＋/- mice and to baseline values. This was consistent with more severe colitis development in these animals. CONCLUSION： Data suggest that defects in SMAD3 signaling increase susceptibility to H. hepaticus-induced colitis through aberrant activation and/or dysregulation of effector lymphoo/tes.展开更多
Objective： To study the effect of three different methods such as medicine, acupuncture and laser acupuncture on the levels of interleukin 2 （IL-2）, inter- leukin 6 （IL-6） and transforming growth factor-β1 （TGF...Objective： To study the effect of three different methods such as medicine, acupuncture and laser acupuncture on the levels of interleukin 2 （IL-2）, inter- leukin 6 （IL-6） and transforming growth factor-β1 （TGF-β1） in rat＇s serum with chronic atrophic gastritis. Methods： 9 rats were randomly selected from 60 rats as normal con- trol group, and the other rats were used to replicate the animal model of chronic atrophic gastritis with comprehensive method, 5 rats were randomly inspected at the 8th week in the process of creating model for gastric mucosa pathological examination; when gastric mucosa shows CAG symptoms such as various degree of congestion, bleeding, atrophy, metaplasia, the model rats were randomly divided into model group, medicine group, acupuncture group and laser acupuncture group. The rats of medicine group were chron- ically administered with yanshenjianwei capsule and Kangfuxin Liquid; the groups of acupuncture and laser acupuncture selected Zusanli acupuncture as acupuncture points, after the treatment for 14 d, blood was removed, double antibody sandwich en- zyme linked immunosorbent assay （ELISA） was used to detect the levels of IL-2, IL-6 and TGF-β1 in serum. Results： Compared with normal group, the contents of IL-2, IL-6 and TGF-β1 in serum of model rats increased （P〈0.05 or P〈0.01）. Medicine, acupuncture and laser acupuncture could reduce the contents of IL-2, IL-6 and TGF-β1, IL-2 in serum of the medicine group and laser acupuncture group was signifi- cantly decreased （P〈0.05）; IL-6 in acupuncture group and laser acupuncture group were significantly decreased （P〈0.05 or P〈0.01）;and the most significant is laser acupuncture group （P〈0.01）. Medicine, acupuncture and laser acupuncture could significantly reduce TGF-β 1 content in serum （P〈0.05 or P〈0.01）, and the particularly significant was laser acupuncture（P〈0.01）. Conclusion： Laser acupuncture has the function of reducing the inflammatory response and adjusting immune function. It earl effectively reduce the ex- pression level of IL-2, IL-6 and TGF- β1 in serum of rats with chronic atrophic gastri- tis, and its effects are better than traditional acupuncture and medicine.展开更多
基金Supported by AgBio Research Center at Michigan State University
文摘AIM： To identify and characterize drosophila mothers against decapentaplegic （SMAD）3-dependent changes in immune cell populations following infection with He- Iicobacter hepaticus （H. hepaticus）. METHODS： SMAD3/ （n = L9） and colitis-resistant SMAD3＋/ （n = 24） mice （8-10 wk of age） were in- fected with/-/, hepaticus and changes in immune cell populations [T lymphocytes, natural killer （NK） cells, T regulatory cells] were measured in the spleen and mesenteric lymph nodes （MsLNs） at 0 d, 3 d, 7 d and 28 d post-infection using flow cytometry. Genotype-dependent changes in T lymphocytes and granzyme B＋ cells were also assessed after 28 d in proximal colon tissue using immunohistochemistry. RESULTS： As previously observed, SMAD3＋, but not SMAD3＋/- mice, developed colitis, peaking at 4 wk post-infection. No significant changes in T cell subsets were observed in the spleen or in the MsLNs between genotypes at any time point. However, CD4＋ and CD8＋/ CD62L＋＋ cells, an effector T lymphocyte population, as well as NK cells （NKp46/DX5＋） were significantly higher in the MsLNs of SMAD3/ mice at 7 d and 28 d post-in- fection. In the colon, a higher number of CD3＋ cells were present in SMAD3＋ compared to SMAD3＋/- mice at base- line, which did not significantly change during infection. However, the number of granzyme B＋ cells, a marker of cytolytic lymphoo/tes, significantly increased in SMAD3＋ mice 28 d post-infection compared to both SMAD3＋/- mice and to baseline values. This was consistent with more severe colitis development in these animals. CONCLUSION： Data suggest that defects in SMAD3 signaling increase susceptibility to H. hepaticus-induced colitis through aberrant activation and/or dysregulation of effector lymphoo/tes.
基金Science and Technology Program of Hebei,China,grant number:14277778D
文摘Objective： To study the effect of three different methods such as medicine, acupuncture and laser acupuncture on the levels of interleukin 2 （IL-2）, inter- leukin 6 （IL-6） and transforming growth factor-β1 （TGF-β1） in rat＇s serum with chronic atrophic gastritis. Methods： 9 rats were randomly selected from 60 rats as normal con- trol group, and the other rats were used to replicate the animal model of chronic atrophic gastritis with comprehensive method, 5 rats were randomly inspected at the 8th week in the process of creating model for gastric mucosa pathological examination; when gastric mucosa shows CAG symptoms such as various degree of congestion, bleeding, atrophy, metaplasia, the model rats were randomly divided into model group, medicine group, acupuncture group and laser acupuncture group. The rats of medicine group were chron- ically administered with yanshenjianwei capsule and Kangfuxin Liquid; the groups of acupuncture and laser acupuncture selected Zusanli acupuncture as acupuncture points, after the treatment for 14 d, blood was removed, double antibody sandwich en- zyme linked immunosorbent assay （ELISA） was used to detect the levels of IL-2, IL-6 and TGF-β1 in serum. Results： Compared with normal group, the contents of IL-2, IL-6 and TGF-β1 in serum of model rats increased （P〈0.05 or P〈0.01）. Medicine, acupuncture and laser acupuncture could reduce the contents of IL-2, IL-6 and TGF-β1, IL-2 in serum of the medicine group and laser acupuncture group was signifi- cantly decreased （P〈0.05）; IL-6 in acupuncture group and laser acupuncture group were significantly decreased （P〈0.05 or P〈0.01）;and the most significant is laser acupuncture group （P〈0.01）. Medicine, acupuncture and laser acupuncture could significantly reduce TGF-β 1 content in serum （P〈0.05 or P〈0.01）, and the particularly significant was laser acupuncture（P〈0.01）. Conclusion： Laser acupuncture has the function of reducing the inflammatory response and adjusting immune function. It earl effectively reduce the ex- pression level of IL-2, IL-6 and TGF- β1 in serum of rats with chronic atrophic gastri- tis, and its effects are better than traditional acupuncture and medicine.
