The expression of the products of IGF-Ⅱ,IGF-Ⅱ receptors(IGF-Ⅱ-R)and CSF-Ⅰ re-ceptors(CSF-Ⅰ-R)was observed in 17 cases of human primary hepatocellular carcinoma(PHC)and the juxtacancerous liver tissue with immunoh...The expression of the products of IGF-Ⅱ,IGF-Ⅱ receptors(IGF-Ⅱ-R)and CSF-Ⅰ re-ceptors(CSF-Ⅰ-R)was observed in 17 cases of human primary hepatocellular carcinoma(PHC)and the juxtacancerous liver tissue with immunohistochemistry(ABC),Western blot and North-ern blot technique,It was found that the expression of IGF-Ⅱ,IGF-Ⅱ-R and CSF-Ⅰ-R was signif-icantly higher in PHC than in normal liver tissue and the expression of IGF-Ⅱ and IGF-Ⅱ-R wasremarkably higher in the juxtacancerous liver tissue from PHC patients than in PHC proper.Itwas noteworthy that the expression of IGF-Ⅱ in both the cancer proper and the juxtacancerousliver tissue was characterized by its fetal type.Besides,the expression of CSF-Ⅰ-R was signifi-cantly higher in PHC than in the juxtacancerous liver tissue.It is believed that the abnormal ex-pression of IGF-Ⅱ,IGF-Ⅱ-R and CSF-I-R in PHC and the juxtacaneerous liver tissue might berelated to the autocrine mechanism of human PHC.展开更多
Objective:To investigate the interference and expression of human glial cell line-derived neurotrophic factor(hCDNF) and soluble TNF alpha(sTMFRⅠ) receptor genes in neural stem cells and to evaluate the roles of thes...Objective:To investigate the interference and expression of human glial cell line-derived neurotrophic factor(hCDNF) and soluble TNF alpha(sTMFRⅠ) receptor genes in neural stem cells and to evaluate the roles of these proteins in the genetic treatment of spinal cord injury.Methods:Full-length of GDNF cDNA(538 bp) and sTMFRⅠcDNA(504 bp) were inserted into the early 1 region of adenovirus genomic DNA respectively and were immediated by the human cytomegalovirus(gene promoter/enhancer). These adenoviruses were propagated in HEK293 cells via homologous recombination for 7-10 days in vivo,then they were used to infect human neural stem ceils.The infection and expression of gene were tested under immunofluorescence.ELISA and Westem-blot after 48 hours.Results:Almost all the cultured cells showed the nestin immunofluorescence positive staining,which was the characteristics of neural stem cell.A great quantity of EGFP and KFP were observed in neural stem cells,which indicated the expression of GDNF and sTMFRⅠ.After transfection of GDNF and sTMFRⅠgenes,many neural stem cells show GFAP and tubulin immunofluorescence positive staining,which meant that most neural stem cells differentiated into neuron at that condition.Conclusions:The infective efficiency of adenovirus is greatly acceptable to neural stem cell,thus adenovirus provide a useful vector for exogenous GDNF and sTMFRⅠgenes expressing in neural stem cells,which is useful for differentiation of neural stem cell.展开更多
Objective: Transforming Growth Factor-β1 (TGF-β1)plays a central role in the process of . growth suppressionof the hepatocytes, and its type II receptor (TGF-β1R II)transfers the signal of growth suppression. In th...Objective: Transforming Growth Factor-β1 (TGF-β1)plays a central role in the process of . growth suppressionof the hepatocytes, and its type II receptor (TGF-β1R II)transfers the signal of growth suppression. In this study,the gene expression of TGF-β1R II in HCC and itsclinical significance was investigated. Methods: Theexpression of TGF-β1R II mRNA in 30 cases Of HCCtissue and the surrounding liver tissue was separatelydetected using reverse transcription-PCR. Results:The positive expression rate of TGF-β1R II mRNA wassignificantly lower in HCC tissue (11/30) than that in thesurrounding liver tissue (23/30) (P<0.01). Further, theless the cancer tissue expressed TGF-β1R II mRNA, themore poorly the tumoral hepatocyte differentiated(P<0.01) and the more portal vein cancer embolusexisted (p=0.0465). Conclusion: The decreaseexpression of TGF-β1 R II mRNA by tumoral hepatocyteresults in the defect of its negative growth regulation,and this may be one of the most important reasons forits carcinogenesis and uncontrolled growth.展开更多
目的:观察β-榄香烯对四氯化碳肝纤维化大鼠TGF-β_1、α-SMA、Col-Ⅰ表达的影响方法:采用CCl_4皮下注射诱导Wistar♂大鼠肝纤维化模型,用β-榄香烯0.1 mL/100g剂量每天腹腔注射8 wk后,用苏木精-伊红染色(HE)和胶原纤维(Masson)染色观...目的:观察β-榄香烯对四氯化碳肝纤维化大鼠TGF-β_1、α-SMA、Col-Ⅰ表达的影响方法:采用CCl_4皮下注射诱导Wistar♂大鼠肝纤维化模型,用β-榄香烯0.1 mL/100g剂量每天腹腔注射8 wk后,用苏木精-伊红染色(HE)和胶原纤维(Masson)染色观察大鼠肝脏病理变化,酶动力法检测肝功能.SP免疫组化法检测肝组织中α-肌动蛋白(α-SMA)、转化生长因子β_1(TGF-β_1)、Ⅰ型胶原(Col-Ⅰ)表达的变化,样本碱水解法检测肝组织中羟脯氨酸(HYP)的含量.结果:8 wk后,正常组、模型组、对照组及治疗组肝组织胶原纤维面积百分比分别为1.22%±0.24%,7.47%±0.81%,5.57%±0.78%,4.33%±0.48%,治疗组与模型组、对照组相比均有显著差异(P<0.01),并且治疗组肝组织纤维化程度分级较模型组逐渐好转,胶原纤维所占面积显著缩小;在肝组织中测得的Col-Ⅰ阳性面积比分别为3.022%±0.553%,9.998%±1.431%,7.554%±0.914%,4.587%±1.008%,治疗组与模型组、对照组相比均有显著差异(P<0.01).α-SMA和TGF-β_1在治疗组和模型组肝组织中的表达也有显著差异(3.172%±0.542% vs 5.605%±1.315%,P<0.01;2.868%±0.554% vs 5.653%±0.9%,P<0.01).结论:β-榄香烯对四氯化碳肝纤维化大鼠具有拮抗作用,主要是通过抑制肝星状细胞激活,降低TGF-β_1,α-SMA在肝组织中的表达,减少细胞外基质在肝脏中的沉积,从而延缓肝纤维化的进程.展开更多
基金This project was financially aided by the National"Seven-Five"Research Funds of China
文摘The expression of the products of IGF-Ⅱ,IGF-Ⅱ receptors(IGF-Ⅱ-R)and CSF-Ⅰ re-ceptors(CSF-Ⅰ-R)was observed in 17 cases of human primary hepatocellular carcinoma(PHC)and the juxtacancerous liver tissue with immunohistochemistry(ABC),Western blot and North-ern blot technique,It was found that the expression of IGF-Ⅱ,IGF-Ⅱ-R and CSF-Ⅰ-R was signif-icantly higher in PHC than in normal liver tissue and the expression of IGF-Ⅱ and IGF-Ⅱ-R wasremarkably higher in the juxtacancerous liver tissue from PHC patients than in PHC proper.Itwas noteworthy that the expression of IGF-Ⅱ in both the cancer proper and the juxtacancerousliver tissue was characterized by its fetal type.Besides,the expression of CSF-Ⅰ-R was signifi-cantly higher in PHC than in the juxtacancerous liver tissue.It is believed that the abnormal ex-pression of IGF-Ⅱ,IGF-Ⅱ-R and CSF-I-R in PHC and the juxtacaneerous liver tissue might berelated to the autocrine mechanism of human PHC.
基金Shenzhen Science and Technology Project(No.201103061)
文摘Objective:To investigate the interference and expression of human glial cell line-derived neurotrophic factor(hCDNF) and soluble TNF alpha(sTMFRⅠ) receptor genes in neural stem cells and to evaluate the roles of these proteins in the genetic treatment of spinal cord injury.Methods:Full-length of GDNF cDNA(538 bp) and sTMFRⅠcDNA(504 bp) were inserted into the early 1 region of adenovirus genomic DNA respectively and were immediated by the human cytomegalovirus(gene promoter/enhancer). These adenoviruses were propagated in HEK293 cells via homologous recombination for 7-10 days in vivo,then they were used to infect human neural stem ceils.The infection and expression of gene were tested under immunofluorescence.ELISA and Westem-blot after 48 hours.Results:Almost all the cultured cells showed the nestin immunofluorescence positive staining,which was the characteristics of neural stem cell.A great quantity of EGFP and KFP were observed in neural stem cells,which indicated the expression of GDNF and sTMFRⅠ.After transfection of GDNF and sTMFRⅠgenes,many neural stem cells show GFAP and tubulin immunofluorescence positive staining,which meant that most neural stem cells differentiated into neuron at that condition.Conclusions:The infective efficiency of adenovirus is greatly acceptable to neural stem cell,thus adenovirus provide a useful vector for exogenous GDNF and sTMFRⅠgenes expressing in neural stem cells,which is useful for differentiation of neural stem cell.
文摘Objective: Transforming Growth Factor-β1 (TGF-β1)plays a central role in the process of . growth suppressionof the hepatocytes, and its type II receptor (TGF-β1R II)transfers the signal of growth suppression. In this study,the gene expression of TGF-β1R II in HCC and itsclinical significance was investigated. Methods: Theexpression of TGF-β1R II mRNA in 30 cases Of HCCtissue and the surrounding liver tissue was separatelydetected using reverse transcription-PCR. Results:The positive expression rate of TGF-β1R II mRNA wassignificantly lower in HCC tissue (11/30) than that in thesurrounding liver tissue (23/30) (P<0.01). Further, theless the cancer tissue expressed TGF-β1R II mRNA, themore poorly the tumoral hepatocyte differentiated(P<0.01) and the more portal vein cancer embolusexisted (p=0.0465). Conclusion: The decreaseexpression of TGF-β1 R II mRNA by tumoral hepatocyteresults in the defect of its negative growth regulation,and this may be one of the most important reasons forits carcinogenesis and uncontrolled growth.
文摘目的:观察β-榄香烯对四氯化碳肝纤维化大鼠TGF-β_1、α-SMA、Col-Ⅰ表达的影响方法:采用CCl_4皮下注射诱导Wistar♂大鼠肝纤维化模型,用β-榄香烯0.1 mL/100g剂量每天腹腔注射8 wk后,用苏木精-伊红染色(HE)和胶原纤维(Masson)染色观察大鼠肝脏病理变化,酶动力法检测肝功能.SP免疫组化法检测肝组织中α-肌动蛋白(α-SMA)、转化生长因子β_1(TGF-β_1)、Ⅰ型胶原(Col-Ⅰ)表达的变化,样本碱水解法检测肝组织中羟脯氨酸(HYP)的含量.结果:8 wk后,正常组、模型组、对照组及治疗组肝组织胶原纤维面积百分比分别为1.22%±0.24%,7.47%±0.81%,5.57%±0.78%,4.33%±0.48%,治疗组与模型组、对照组相比均有显著差异(P<0.01),并且治疗组肝组织纤维化程度分级较模型组逐渐好转,胶原纤维所占面积显著缩小;在肝组织中测得的Col-Ⅰ阳性面积比分别为3.022%±0.553%,9.998%±1.431%,7.554%±0.914%,4.587%±1.008%,治疗组与模型组、对照组相比均有显著差异(P<0.01).α-SMA和TGF-β_1在治疗组和模型组肝组织中的表达也有显著差异(3.172%±0.542% vs 5.605%±1.315%,P<0.01;2.868%±0.554% vs 5.653%±0.9%,P<0.01).结论:β-榄香烯对四氯化碳肝纤维化大鼠具有拮抗作用,主要是通过抑制肝星状细胞激活,降低TGF-β_1,α-SMA在肝组织中的表达,减少细胞外基质在肝脏中的沉积,从而延缓肝纤维化的进程.
