An amphotropic pseudotype retrovirus containing human N-ras antisense gene was constructed and packaged with helper cells. It has been previously demonstrated that the virus did inhibit the growth of human hepatocarci...An amphotropic pseudotype retrovirus containing human N-ras antisense gene was constructed and packaged with helper cells. It has been previously demonstrated that the virus did inhibit the growth of human hepatocarcinoma cell line PLC PRF/5 in vitro accompanied with the blockage of p21 expression. Based on these results, further study was carried on to examine the effect of these viruses on the growth of human hepatoma transplanted LTNM4 in nude mice. It has been shown that the retrovirus containing human antisense N-ras gene could inhibit the hepatoma in nude mice at a rate of 78% (P<0.05) as compared with saline control. No inhibition was observed in group treated with retrovirus which contained no N-ras sequence. These results in vivo lend further support that human N-ras antisense gene mediated by retrovirus could block the expression of the relevant oncogene and lead to the inhibition of cancer growth. It also provided the basis for further approaches of gene therapy for human cancer.展开更多
Controversy still exists in the liver transplantation as one of the surgical treatments for hepatic cancer.Of the 14cases of orthotopic liver transplantation performed in our institute since 1977,12 cases were primary...Controversy still exists in the liver transplantation as one of the surgical treatments for hepatic cancer.Of the 14cases of orthotopic liver transplantation performed in our institute since 1977,12 cases were primary liver cancer(PLC).No intraoperative and immediate postoperative death occured.Four cases survived for over 3 months,in whom 2 survived for over half a year,and 1 nearly 9 months.The main causes of death were liver failure,rejection, tumor recurrence and infection.The results and the indication of liver transplantation for PLC are discussed.The liver cancer classification more suitable for PLC and selection of the surgical treatment are suggested. It is hold that the liver transplantation is justified for the PLC at stageⅠ,type B and stageⅡ.The reasons why the results of the liver transplantation were poor in China and in our institute are analyzed.It is believed that better results will be gained by means of more strict case selection,immprovement of surgical technique and Cyclosporin A(CsA)application.展开更多
We present a video case of a 51-year-old man admitted to our surgical and liver transplantation unit for hepatocellular cancer（HCC）. Patient has a HCV cirrhosis with portal hypertension and esophageal varices F1. Ch...We present a video case of a 51-year-old man admitted to our surgical and liver transplantation unit for hepatocellular cancer（HCC）. Patient has a HCV cirrhosis with portal hypertension and esophageal varices F1. Child Pugh score was B7 and model of end staged liver disease（MELD） was 11. Body mass index（BMI） was 26.7 and ASA score was 2. No previous abdominal surgery. According with our multidisciplinary group we suggest a laparoscopic left lobectomy for the patient. Pringle manoeuvre was not performed. Operation time was 193 min and blood loss estimation was 100 cc. No transfusion was required. Postoperative course was uneventful, grade I of Clavien-Dindo Classification. Patient was discharged in day 8. In our experience laparoscopic resection in cirrhotic liver should be performed in selected patients and in an experienced team.展开更多
文摘An amphotropic pseudotype retrovirus containing human N-ras antisense gene was constructed and packaged with helper cells. It has been previously demonstrated that the virus did inhibit the growth of human hepatocarcinoma cell line PLC PRF/5 in vitro accompanied with the blockage of p21 expression. Based on these results, further study was carried on to examine the effect of these viruses on the growth of human hepatoma transplanted LTNM4 in nude mice. It has been shown that the retrovirus containing human antisense N-ras gene could inhibit the hepatoma in nude mice at a rate of 78% (P<0.05) as compared with saline control. No inhibition was observed in group treated with retrovirus which contained no N-ras sequence. These results in vivo lend further support that human N-ras antisense gene mediated by retrovirus could block the expression of the relevant oncogene and lead to the inhibition of cancer growth. It also provided the basis for further approaches of gene therapy for human cancer.
文摘Controversy still exists in the liver transplantation as one of the surgical treatments for hepatic cancer.Of the 14cases of orthotopic liver transplantation performed in our institute since 1977,12 cases were primary liver cancer(PLC).No intraoperative and immediate postoperative death occured.Four cases survived for over 3 months,in whom 2 survived for over half a year,and 1 nearly 9 months.The main causes of death were liver failure,rejection, tumor recurrence and infection.The results and the indication of liver transplantation for PLC are discussed.The liver cancer classification more suitable for PLC and selection of the surgical treatment are suggested. It is hold that the liver transplantation is justified for the PLC at stageⅠ,type B and stageⅡ.The reasons why the results of the liver transplantation were poor in China and in our institute are analyzed.It is believed that better results will be gained by means of more strict case selection,immprovement of surgical technique and Cyclosporin A(CsA)application.
文摘We present a video case of a 51-year-old man admitted to our surgical and liver transplantation unit for hepatocellular cancer（HCC）. Patient has a HCV cirrhosis with portal hypertension and esophageal varices F1. Child Pugh score was B7 and model of end staged liver disease（MELD） was 11. Body mass index（BMI） was 26.7 and ASA score was 2. No previous abdominal surgery. According with our multidisciplinary group we suggest a laparoscopic left lobectomy for the patient. Pringle manoeuvre was not performed. Operation time was 193 min and blood loss estimation was 100 cc. No transfusion was required. Postoperative course was uneventful, grade I of Clavien-Dindo Classification. Patient was discharged in day 8. In our experience laparoscopic resection in cirrhotic liver should be performed in selected patients and in an experienced team.
