[Objectives]To screen the tissue culture rapid propagation formula suitable for each tissue culture stage of Anoectochilus roxburghii( Wall) Lind. [Methods] The stem segments of Fujian A. roxburghii were used as expla...[Objectives]To screen the tissue culture rapid propagation formula suitable for each tissue culture stage of Anoectochilus roxburghii( Wall) Lind. [Methods] The stem segments of Fujian A. roxburghii were used as explant to study the tissue culture rapid propagation and transplantation techniques. The comparative experiment was carried out to study the effects of different hormone concentrations on the induction of stem segments,proliferation of cluster buds,rooting and seedling hardening of A. roxburghii,and study the effects of transplantation matrix on the transplantation of A. roxburghii. [Results]MS + 0. 5 mg/L NAA + 2 mg/L BA + 20 g/L sucrose + 6 g/L agar was suitable for induction of stem segments of A. roxburghii; MS + 0. 5 mg/L NAA + 2 mg/L BA + 1 mg/L KT + 25 g/L sucrose + 6 g/L agar was most suitable for proliferation of cluster buds of A. roxburghii; MS + 1. 0 mg/L IBA + 1. 0 mg/L NAA + 1 g/L activated carbon + 50 g/L mashed banana + 25 g/L sucrose + 6 g/L agar was most suitable for rooting and seedling hardening of A. roxburghii; using peat soil: fine sand( 3∶ 1) as transplantation matrix,the survival rate was the highest. [Conclusions] The experiment results are expected to provide references for factory production of A. roxburghii.展开更多
Domino liver transplantation（LT）, using livers from familial amyloidotic polyneuropathy（FAP） patients, is a well described technique useful to expand donor pool. One of the main difficulties of this type of LT ari...Domino liver transplantation（LT）, using livers from familial amyloidotic polyneuropathy（FAP） patients, is a well described technique useful to expand donor pool. One of the main difficulties of this type of LT arises from the necessity to share the vascular pedicles between the graft and the donor. The most important challenge resides in restoring a proper hepatic venous outflow in the FAP-liver recipient.This is specially challenging when using the piggy-back technique, because the hepatic stumps may be too short. To overcome this issue, surgeons explored several techniques using different types of venous grafts. We describe a new technical option by using an arterial graft from the deceased donor. By using both iliac arteries a long graft is created and sutured as needed to the hepatic vein stump. We describe herein this new technique employed in a domino liver recipient who underwent retransplantation for ischemic cholangitis. The procedure was performed using the piggy-back technique; the venous stump of the FAP liver was reconstructed with the arterial graft. The patient had uneventful postoperative and mid-term hepatic function, and anastomosis was patent 24 months after LT.展开更多
Background:Indoor air quality(IAQ)is a concern in kindergartens as children spend much of their time there.Yet,there is a shortage of biological indicators needed for assessing IAQ.Thus,this study evaluated IAQ using ...Background:Indoor air quality(IAQ)is a concern in kindergartens as children spend much of their time there.Yet,there is a shortage of biological indicators needed for assessing IAQ.Thus,this study evaluated IAQ using transplanted lichen Usnea misaminensis as a biological indicator.Methods:Lichen samples,collected from Bukit Larut,Perak,Malaysia,were exposed to indoor and outdoor environments in an urban area(Ummi Aiman Kindergarten)and a rural area(Ummi Qaseh Pelangi Kindergarten)for 2 months during August 15 to October 14,2019.The concentrations of 12 selected elements and the vitality of the lichens were then evaluated.Results:Increased concentrations of eleven of the twelve elements deposited in the lichen samples in both urban and rural areas were observed.For both areas,the element concentrations in the samples from the indoor environment was lower than those from the outdoor environment,and those in the rural area were lower than those from in the urban area,suggesting the impacts of traffic emissions.The vitality of the lichens showed no significant change in indoor environment,compared to that in outdoor environment,indicating that even exposed to indoor environment,the lichens remained effective biological indicators as same as they were in the outdoor environment.Conclusions:Lichens are effective biological indicators for both outdoor and indoor environments.Furthermore,outdoor emissions could influence IAQ,which could be problematic in densely populated areas such as kindergartens.Mitigation measures should be taken.展开更多
基金Supported by Science and Technology Project of Huizhou City(2014C040007001)
文摘[Objectives]To screen the tissue culture rapid propagation formula suitable for each tissue culture stage of Anoectochilus roxburghii( Wall) Lind. [Methods] The stem segments of Fujian A. roxburghii were used as explant to study the tissue culture rapid propagation and transplantation techniques. The comparative experiment was carried out to study the effects of different hormone concentrations on the induction of stem segments,proliferation of cluster buds,rooting and seedling hardening of A. roxburghii,and study the effects of transplantation matrix on the transplantation of A. roxburghii. [Results]MS + 0. 5 mg/L NAA + 2 mg/L BA + 20 g/L sucrose + 6 g/L agar was suitable for induction of stem segments of A. roxburghii; MS + 0. 5 mg/L NAA + 2 mg/L BA + 1 mg/L KT + 25 g/L sucrose + 6 g/L agar was most suitable for proliferation of cluster buds of A. roxburghii; MS + 1. 0 mg/L IBA + 1. 0 mg/L NAA + 1 g/L activated carbon + 50 g/L mashed banana + 25 g/L sucrose + 6 g/L agar was most suitable for rooting and seedling hardening of A. roxburghii; using peat soil: fine sand( 3∶ 1) as transplantation matrix,the survival rate was the highest. [Conclusions] The experiment results are expected to provide references for factory production of A. roxburghii.
文摘Domino liver transplantation（LT）, using livers from familial amyloidotic polyneuropathy（FAP） patients, is a well described technique useful to expand donor pool. One of the main difficulties of this type of LT arises from the necessity to share the vascular pedicles between the graft and the donor. The most important challenge resides in restoring a proper hepatic venous outflow in the FAP-liver recipient.This is specially challenging when using the piggy-back technique, because the hepatic stumps may be too short. To overcome this issue, surgeons explored several techniques using different types of venous grafts. We describe a new technical option by using an arterial graft from the deceased donor. By using both iliac arteries a long graft is created and sutured as needed to the hepatic vein stump. We describe herein this new technique employed in a domino liver recipient who underwent retransplantation for ischemic cholangitis. The procedure was performed using the piggy-back technique; the venous stump of the FAP liver was reconstructed with the arterial graft. The patient had uneventful postoperative and mid-term hepatic function, and anastomosis was patent 24 months after LT.
基金supported by Universiti Kebangsaan Malaysia through a research grant(GUP-2018-032).
文摘Background:Indoor air quality(IAQ)is a concern in kindergartens as children spend much of their time there.Yet,there is a shortage of biological indicators needed for assessing IAQ.Thus,this study evaluated IAQ using transplanted lichen Usnea misaminensis as a biological indicator.Methods:Lichen samples,collected from Bukit Larut,Perak,Malaysia,were exposed to indoor and outdoor environments in an urban area(Ummi Aiman Kindergarten)and a rural area(Ummi Qaseh Pelangi Kindergarten)for 2 months during August 15 to October 14,2019.The concentrations of 12 selected elements and the vitality of the lichens were then evaluated.Results:Increased concentrations of eleven of the twelve elements deposited in the lichen samples in both urban and rural areas were observed.For both areas,the element concentrations in the samples from the indoor environment was lower than those from the outdoor environment,and those in the rural area were lower than those from in the urban area,suggesting the impacts of traffic emissions.The vitality of the lichens showed no significant change in indoor environment,compared to that in outdoor environment,indicating that even exposed to indoor environment,the lichens remained effective biological indicators as same as they were in the outdoor environment.Conclusions:Lichens are effective biological indicators for both outdoor and indoor environments.Furthermore,outdoor emissions could influence IAQ,which could be problematic in densely populated areas such as kindergartens.Mitigation measures should be taken.
