目的:探讨呼吸道合胞病毒(respiratory syncytial virus,RSV)感染导致哮喘易感性增加的机制,观察人支气管上皮细胞感染RSV后IL-8的表达,以及IL-8对Th17/调节性T淋巴细胞(regulatory T cells,Treg)分化的调节作用。方法:将人支气管上皮细...目的:探讨呼吸道合胞病毒(respiratory syncytial virus,RSV)感染导致哮喘易感性增加的机制,观察人支气管上皮细胞感染RSV后IL-8的表达,以及IL-8对Th17/调节性T淋巴细胞(regulatory T cells,Treg)分化的调节作用。方法:将人支气管上皮细胞(human bronchial epithelial cells,HBECs)分为对照组和RSV感染组,构建并验证RSV持续感染HBECs模型,real-time PCR检测对照组和RSV感染组中IL-8 m RNA的表达;ELISA检测感染细胞上清液中IL-8的浓度。提取健康人外周血淋巴细胞并将其分为空白对照组和IL-8作用组,参照ELISA检测到的浓度将IL-8作用于淋巴细胞24 h,采用流式细胞仪检测淋巴细胞中Th17和Treg亚群的分布情况。结果:本实验成功构建RSV持续感染HBECs模型,感染后的细胞仍能够继续分裂传代,并可检测到RSV持续存在的证据。免疫荧光显示细胞内有RSV致病蛋白的荧光表达;电镜下观察到感染细胞的线粒体水肿和内质网扩张,出现核周裂隙以及合胞现象,细胞核、胞浆内有病毒颗粒分布。Real-time PCR和ELISA分别检测到RSV感染组细胞内IL-8 m RNA表达和上清液中IL-8的分泌水平均高于对照组(均P<0.05)。进一步将IL-8作用于淋巴细胞,流式细胞学结果表明IL-8作用组淋巴细胞中Th17亚群比率增高(P<0.05),但Treg亚群比率与对照组比较,差异无统计学意义(P>0.05)。结论:RSV感染气道上皮细胞后可通过过度分泌IL-8而引起Th 17/Treg亚群分化异常。展开更多
AIM To investigate the role of regulatory T cell(Treg) subsets in the balance between Treg and T helper 17(Th17) cells in various tissues from mice with dextran sulfate sodium-induced colitis.METHODS T r e g c e l l s...AIM To investigate the role of regulatory T cell(Treg) subsets in the balance between Treg and T helper 17(Th17) cells in various tissues from mice with dextran sulfate sodium-induced colitis.METHODS T r e g c e l l s, T r e g c e l l s u b s e t s, T h 1 7 c e l l s, a n d CD4+CD25+FoxP 3+IL-17+ cells from the lamina propria of colon(LPC) and other ulcerative colitis(UC) mouse tissues were evaluated by flow cytometry. Forkhead box protein 3(FoxP 3), interleukin 17A(IL-17A), and RORC m RNA levels were assessed by real-time PCR, while interleukin-10(IL-10) and IL-17 A levels were detected with a Cytometric Beads Array.RESULTS In peripheral blood monocytes(PBMC), mesenteric lymphnode(MLN), lamina propria of jejunum(LPJ) and LPC from UC mice, Treg cell numbers were increased(P < 0.05), and FoxP 3 and IL-10 mR NA levels were decreased. Th17 cell numbers were also increased in PBMC and LPC, as were IL-17 A levels in PBMC, LPJ, and serum. The number of FrI subset cells(CD4+CD45RA+FoxP 3low) was increased in the spleen, MLN, LPJ, and LPC. FrI I subset cells(CD4+CD45RA-Fox P3high) were decreased among PBMC, MLN, LPJ, and LPC, but the number of Fr III cells(CD4+CD45RA-FoxP 3low) and CD4+CD25+FoxP 3+IL-17A+ cells was increased. Fox P3 m RNA levels in CD4+CD45RA-Fox P3 low cells decreased in PBMC, MLN, LPJ, and LPC in UC mice, while IL-17 A and RORC mR NA increased. In UC mice the distribution of Treg, Th17 cells, CD4+CD45RA-FoxP 3high, and CD4+CD45RA-FoxP 3low cells was higher in LPC relative to other tissues.CONCLUSION Increased numbers of CD4+CD45RA-FoxP 3low cells may cause an imbalance between Treg and Th17 cells that is mainly localized to the LPC rather than secondary lymphoid tissues.展开更多
目的:探讨晚期非小细胞肺癌(non small cell lung cancer,NSCLC)患者外周血淋巴细胞亚群与预后的关系。方法:采用流式细胞仪技术检测120例晚期NSCLC(其中Ⅲb期和Ⅳ期患者各60例)及60例健康体检者外周血T淋巴细胞亚群占外周血单个核细胞...目的:探讨晚期非小细胞肺癌(non small cell lung cancer,NSCLC)患者外周血淋巴细胞亚群与预后的关系。方法:采用流式细胞仪技术检测120例晚期NSCLC(其中Ⅲb期和Ⅳ期患者各60例)及60例健康体检者外周血T淋巴细胞亚群占外周血单个核细胞的比例,单因素分析淋巴细胞亚群与患者生存的关系,并对患者的临床病理资料进行回顾性分析。结果:120例晚期NSCLC患者CD3+T细胞和NK细胞比例低于健康对照,调节性细胞(regular T cell,Treg)高于健康对照,差异有统计学意义(P<0.05)。CD4+T细胞和CD8+T细胞亦低于健康对照,差别无统计学意义。单因素分析显示,Treg细胞是影响患者预后的因素之一。临床病理资料分析显示,肿瘤分化越差,分期越高,Treg细胞数量越多,差别有统计学意义,P<0.05。结论:晚期NSCLC患者外周血Treg细胞随疾病进展而增高,Treg细胞在一定程度上反映患者的预后。展开更多
基金Supported by the National Natural Science Foundation of China,No.81300294State Scholarship Fund of China,No.201509110033
文摘AIM To investigate the role of regulatory T cell(Treg) subsets in the balance between Treg and T helper 17(Th17) cells in various tissues from mice with dextran sulfate sodium-induced colitis.METHODS T r e g c e l l s, T r e g c e l l s u b s e t s, T h 1 7 c e l l s, a n d CD4+CD25+FoxP 3+IL-17+ cells from the lamina propria of colon(LPC) and other ulcerative colitis(UC) mouse tissues were evaluated by flow cytometry. Forkhead box protein 3(FoxP 3), interleukin 17A(IL-17A), and RORC m RNA levels were assessed by real-time PCR, while interleukin-10(IL-10) and IL-17 A levels were detected with a Cytometric Beads Array.RESULTS In peripheral blood monocytes(PBMC), mesenteric lymphnode(MLN), lamina propria of jejunum(LPJ) and LPC from UC mice, Treg cell numbers were increased(P < 0.05), and FoxP 3 and IL-10 mR NA levels were decreased. Th17 cell numbers were also increased in PBMC and LPC, as were IL-17 A levels in PBMC, LPJ, and serum. The number of FrI subset cells(CD4+CD45RA+FoxP 3low) was increased in the spleen, MLN, LPJ, and LPC. FrI I subset cells(CD4+CD45RA-Fox P3high) were decreased among PBMC, MLN, LPJ, and LPC, but the number of Fr III cells(CD4+CD45RA-FoxP 3low) and CD4+CD25+FoxP 3+IL-17A+ cells was increased. Fox P3 m RNA levels in CD4+CD45RA-Fox P3 low cells decreased in PBMC, MLN, LPJ, and LPC in UC mice, while IL-17 A and RORC mR NA increased. In UC mice the distribution of Treg, Th17 cells, CD4+CD45RA-FoxP 3high, and CD4+CD45RA-FoxP 3low cells was higher in LPC relative to other tissues.CONCLUSION Increased numbers of CD4+CD45RA-FoxP 3low cells may cause an imbalance between Treg and Th17 cells that is mainly localized to the LPC rather than secondary lymphoid tissues.
文摘目的:探讨晚期非小细胞肺癌(non small cell lung cancer,NSCLC)患者外周血淋巴细胞亚群与预后的关系。方法:采用流式细胞仪技术检测120例晚期NSCLC(其中Ⅲb期和Ⅳ期患者各60例)及60例健康体检者外周血T淋巴细胞亚群占外周血单个核细胞的比例,单因素分析淋巴细胞亚群与患者生存的关系,并对患者的临床病理资料进行回顾性分析。结果:120例晚期NSCLC患者CD3+T细胞和NK细胞比例低于健康对照,调节性细胞(regular T cell,Treg)高于健康对照,差异有统计学意义(P<0.05)。CD4+T细胞和CD8+T细胞亦低于健康对照,差别无统计学意义。单因素分析显示,Treg细胞是影响患者预后的因素之一。临床病理资料分析显示,肿瘤分化越差,分期越高,Treg细胞数量越多,差别有统计学意义,P<0.05。结论:晚期NSCLC患者外周血Treg细胞随疾病进展而增高,Treg细胞在一定程度上反映患者的预后。
