目的分析梅毒诊断中应用梅毒螺旋体明胶颗粒凝集试验(treponema pallidum particle assay,TPPA)与梅毒血浆反应素快速试验(rapid plasma reagin test for syphilis,RPR)检测的诊断效果。方法选取聊城市人民医院2020年8月—2022年8月收...目的分析梅毒诊断中应用梅毒螺旋体明胶颗粒凝集试验(treponema pallidum particle assay,TPPA)与梅毒血浆反应素快速试验(rapid plasma reagin test for syphilis,RPR)检测的诊断效果。方法选取聊城市人民医院2020年8月—2022年8月收治的疑似梅毒患者97例进行研究,同时给予RPR与TPPA检测,以梅毒螺旋体显微镜检为金标准,分析RPR与TPPA检测的各项诊断效能值。结果97例患者梅毒螺旋体显微镜检结果为32例阳性,TPPA检查显示44例阳性(真阳性31例),灵敏度为96.88%、特异度为80.00%、准确度为85.57%;RPR检查显示41例阳性(真阳性29例),灵敏度为90.63%、特异度为81.54%、准确度为84.54%;两组数据比较,差异无统计学意义(χ^(2)=1.000、0.200、0.111,P>0.05)。结论RPR与TPPA均可作为诊断梅毒患者的有效方法,TPPA试验与金标准诊断结果接近,可发挥良好作用。展开更多
The methods and strategies used to screen for syp-hilis and to confirm initially reactive results can vary significantly across clinical laboratories. While the performance characteristics of these different appro-ach...The methods and strategies used to screen for syp-hilis and to confirm initially reactive results can vary significantly across clinical laboratories. While the performance characteristics of these different appro-aches have been evaluated by multiple studies, there is not, as of yet, a single, universally recommendedalgorithm for syphilis testing. To clarify the currently available options for syphilis testing, this update will summarize the clinical challenges to diagnosis, review the specific performance characteristics of treponemal and non-treponemal tests, and fnally, summarize select studies published over the past decade which have evaluated these approaches. Specifcally, this review will discuss the traditional and reverse sequence syphilis screening algorithms commonly used in the United States, alongside a discussion of the European Centre for Disease Prevention and Control syphilis algorithm. Ultimately, in the United States, the decision of which algorithm to use is largely dependent on laboratory resources, the local incidence of syphilis and patient demographics. Key words： Syphilis; Treponemal infection; Immuno-assay; Reverse sequence screening; Rapid plasma regain; Treponema pallidum particle agglutination test; Automation; Algorithm; Primary infection; Late latent infection展开更多
文摘目的分析梅毒诊断中应用梅毒螺旋体明胶颗粒凝集试验(treponema pallidum particle assay,TPPA)与梅毒血浆反应素快速试验(rapid plasma reagin test for syphilis,RPR)检测的诊断效果。方法选取聊城市人民医院2020年8月—2022年8月收治的疑似梅毒患者97例进行研究,同时给予RPR与TPPA检测,以梅毒螺旋体显微镜检为金标准,分析RPR与TPPA检测的各项诊断效能值。结果97例患者梅毒螺旋体显微镜检结果为32例阳性,TPPA检查显示44例阳性(真阳性31例),灵敏度为96.88%、特异度为80.00%、准确度为85.57%;RPR检查显示41例阳性(真阳性29例),灵敏度为90.63%、特异度为81.54%、准确度为84.54%;两组数据比较,差异无统计学意义(χ^(2)=1.000、0.200、0.111,P>0.05)。结论RPR与TPPA均可作为诊断梅毒患者的有效方法,TPPA试验与金标准诊断结果接近,可发挥良好作用。
文摘The methods and strategies used to screen for syp-hilis and to confirm initially reactive results can vary significantly across clinical laboratories. While the performance characteristics of these different appro-aches have been evaluated by multiple studies, there is not, as of yet, a single, universally recommendedalgorithm for syphilis testing. To clarify the currently available options for syphilis testing, this update will summarize the clinical challenges to diagnosis, review the specific performance characteristics of treponemal and non-treponemal tests, and fnally, summarize select studies published over the past decade which have evaluated these approaches. Specifcally, this review will discuss the traditional and reverse sequence syphilis screening algorithms commonly used in the United States, alongside a discussion of the European Centre for Disease Prevention and Control syphilis algorithm. Ultimately, in the United States, the decision of which algorithm to use is largely dependent on laboratory resources, the local incidence of syphilis and patient demographics. Key words： Syphilis; Treponemal infection; Immuno-assay; Reverse sequence screening; Rapid plasma regain; Treponema pallidum particle agglutination test; Automation; Algorithm; Primary infection; Late latent infection
