In the subtribe Maydeae, Tripsacum and Zea are closely related genera. Tripsacum is a horticultural crop widely used as pasture forage. Previous studies suggested that Tripsacum might play an important role in maize o...In the subtribe Maydeae, Tripsacum and Zea are closely related genera. Tripsacum is a horticultural crop widely used as pasture forage. Previous studies suggested that Tripsacum might play an important role in maize origin and evolution. However, our understanding of the genomics and the evolution of Tripsacum remains limited. In this study, two diploids,T. dactyloides var. meridionale(2n = 36, MR) and T. dactyloides(2n = 36, DD), and one tetraploid,T. dactyloides(2n = 72, DL) were sequenced by low-coverage genome sequencing followed by graph-based cluster analysis. The results showed that 63.23%, 59.20%, and 61.57% of the respective genome of MR, DD, and DL were repetitive DNA sequence. The proportions of different repetitive sequences varied greatly among the three species. Fluorescence in situ hybridization(FISH) analysis of mitotic metaphase chromosomes with satellite repeats as the probes showed that the FISH signal patterns of DL were more similar to that of DD than to that of MR. Comparative analysis of the repeats also showed that DL shared more common repeat families with DD than with MR. Phylogenetic analysis of internal transcribed spacer region sequences further supported the evolutionary relationship among the three species. Repetitive sequences comparison showed that Tripsacum shared more repeat families with Zea than with Coix and Sorghum. Our study sheds new light on the genomics of Tripsacum and differential speciation in the Poaceae family.展开更多
RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 3...RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides (male parent) specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.展开更多
多色基因组原位杂交(multi-color genomic in situ hybridization,McGISH)鉴定结果表明,MTPP-58有58条染色体,其中11条染色体来自玉米,28条染色体来自四倍体多年生大刍草,17条染色体来自指状摩擦禾和2条玉米与四倍体多年生大刍草易位的...多色基因组原位杂交(multi-color genomic in situ hybridization,McGISH)鉴定结果表明,MTPP-58有58条染色体,其中11条染色体来自玉米,28条染色体来自四倍体多年生大刍草,17条染色体来自指状摩擦禾和2条玉米与四倍体多年生大刍草易位的染色体。2012-2013年对MTPP-58的饲草产量、品质、越冬率以及繁殖特性研究表明,第1年鲜、干草产量分别为93.845t/hm2和22.083t/hm2,第2年鲜、干草产量分别为82.851t/hm2和14.394t/hm2;粗蛋白(CP)为10.48%,干茎叶比为0.71,越冬率为100%。MTPP-58下部茎干扦插成活率为51.69%,分株成活率为95.50%。多种属聚合远缘杂交创制的MTPP-58聚合了玉米、大刍草、摩擦禾的优良特性,植株根系发达,生长繁茂,分蘖和抗寒性强,生产性能优,可无性繁殖,是一种多种属间杂交创制的新型多年生饲草。展开更多
Tripsacum dactyloides (L.) L., commonly known as eastern gamagrass, is useful for grazing, stored forage, soil amelioration and conservation, and as a biofuel feedstock. Our goal was to calculate accumulated growing d...Tripsacum dactyloides (L.) L., commonly known as eastern gamagrass, is useful for grazing, stored forage, soil amelioration and conservation, and as a biofuel feedstock. Our goal was to calculate accumulated growing degree days (GDD) from existing datasets collected for eastern gamagrass forage production experiments in northwestern Oklahoma, and discuss the use of GDD, instead of calendar harvest dates, in the production of eastern gamagrass forage. Growing degree days were calculated from 1 January each year using the “optimum day method”. For 10 harvest years, the first eastern gamagrass harvest required 690 ± 26 cumulative GDD. Based on long-term weather data from Woodward, Oklahoma, this would place the first harvest on or near 1 June. The second harvest required 635 ± 27 cumulative GDD which would place the second harvest on or near 15 July and the third harvest required 690 ± 23 cumulative GDD placing it on or near 30 August. Each of the 30 harvest required an average of 670 ± 15 cumulative GDD. Using GDD to predict harvest events is a useful tool that forage producer can use in the production of eastern gamagrass forage in the USA and possibly elsewhere.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 31471499, 91535206)
文摘In the subtribe Maydeae, Tripsacum and Zea are closely related genera. Tripsacum is a horticultural crop widely used as pasture forage. Previous studies suggested that Tripsacum might play an important role in maize origin and evolution. However, our understanding of the genomics and the evolution of Tripsacum remains limited. In this study, two diploids,T. dactyloides var. meridionale(2n = 36, MR) and T. dactyloides(2n = 36, DD), and one tetraploid,T. dactyloides(2n = 72, DL) were sequenced by low-coverage genome sequencing followed by graph-based cluster analysis. The results showed that 63.23%, 59.20%, and 61.57% of the respective genome of MR, DD, and DL were repetitive DNA sequence. The proportions of different repetitive sequences varied greatly among the three species. Fluorescence in situ hybridization(FISH) analysis of mitotic metaphase chromosomes with satellite repeats as the probes showed that the FISH signal patterns of DL were more similar to that of DD than to that of MR. Comparative analysis of the repeats also showed that DL shared more common repeat families with DD than with MR. Phylogenetic analysis of internal transcribed spacer region sequences further supported the evolutionary relationship among the three species. Repetitive sequences comparison showed that Tripsacum shared more repeat families with Zea than with Coix and Sorghum. Our study sheds new light on the genomics of Tripsacum and differential speciation in the Poaceae family.
文摘RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides (male parent) specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.
文摘多色基因组原位杂交(multi-color genomic in situ hybridization,McGISH)鉴定结果表明,MTPP-58有58条染色体,其中11条染色体来自玉米,28条染色体来自四倍体多年生大刍草,17条染色体来自指状摩擦禾和2条玉米与四倍体多年生大刍草易位的染色体。2012-2013年对MTPP-58的饲草产量、品质、越冬率以及繁殖特性研究表明,第1年鲜、干草产量分别为93.845t/hm2和22.083t/hm2,第2年鲜、干草产量分别为82.851t/hm2和14.394t/hm2;粗蛋白(CP)为10.48%,干茎叶比为0.71,越冬率为100%。MTPP-58下部茎干扦插成活率为51.69%,分株成活率为95.50%。多种属聚合远缘杂交创制的MTPP-58聚合了玉米、大刍草、摩擦禾的优良特性,植株根系发达,生长繁茂,分蘖和抗寒性强,生产性能优,可无性繁殖,是一种多种属间杂交创制的新型多年生饲草。
文摘Tripsacum dactyloides (L.) L., commonly known as eastern gamagrass, is useful for grazing, stored forage, soil amelioration and conservation, and as a biofuel feedstock. Our goal was to calculate accumulated growing degree days (GDD) from existing datasets collected for eastern gamagrass forage production experiments in northwestern Oklahoma, and discuss the use of GDD, instead of calendar harvest dates, in the production of eastern gamagrass forage. Growing degree days were calculated from 1 January each year using the “optimum day method”. For 10 harvest years, the first eastern gamagrass harvest required 690 ± 26 cumulative GDD. Based on long-term weather data from Woodward, Oklahoma, this would place the first harvest on or near 1 June. The second harvest required 635 ± 27 cumulative GDD which would place the second harvest on or near 15 July and the third harvest required 690 ± 23 cumulative GDD placing it on or near 30 August. Each of the 30 harvest required an average of 670 ± 15 cumulative GDD. Using GDD to predict harvest events is a useful tool that forage producer can use in the production of eastern gamagrass forage in the USA and possibly elsewhere.
