Objective: To observe the effect and adverse reaction of tripterygium polyglycosides (TP) combined with small dose of methotrexate (MTX) in treating senile rheumatoid arthritis (RA). Methods: Sixty old patients with R...Objective: To observe the effect and adverse reaction of tripterygium polyglycosides (TP) combined with small dose of methotrexate (MTX) in treating senile rheumatoid arthritis (RA). Methods: Sixty old patients with RA were equally divided into two groups, the treated group was treated with oral intake of TP (10 mg three times per day) plus MTX (7.5 mg, once per week), and the control group was treated with MTX (15 mg per week) respectively for 3 months. The changes from before to after treatment in resting pain, morning rigidity, bilateral grip strength, local tenderness index, arthroncus index, patient's evaluation, doctor's evaluation, erythrocyte sedimentation rate (ESR), C-reaction protein (CRP) and rheumatoid factor(RF) titre were observed and the clinical therapeutic effect assessed. Results: The total effective rate in the treated group was 86.67% and that in the control group 83.33% , showing insignificant difference between them (P>0.05). But the improvement in local tenderness index, arthroncus index, morning rigidity and ESR in the treated group was significantly better than those in the control group (P<0.01 or P<0.05). The occurrence rate of adverse reaction in the treated group was 26. 67% , which was lower than that in the control group (43.33%), showing significant difference (P<0.01). Conclusion: TP combined with small dose of MTX is a favorable therapeutic method for treating senile RA.展开更多
Objective： To investigate the effect of Tripterygium polyglycosid on establishing airway eosinophil infiltration and related airway hyperresponsiveness of asthmatic mice. Methods： A mature murine asthmatic model was...Objective： To investigate the effect of Tripterygium polyglycosid on establishing airway eosinophil infiltration and related airway hyperresponsiveness of asthmatic mice. Methods： A mature murine asthmatic model was made with ovabulmin sensitized and challenged C57BL/6 mice. Forty mice were divided into four groups with 10 mice in each group： mice sensitized and challenged with saline （WS group）, mice sensitized and challenged with ovalbumin （WO group）, mice sensitized and challenged with ovalbumin and treated with Tripterygium polyglycosid （TP group） and Dexamethasone （DXM group）. The mice were intraperitoneally injected with 20 I^g chicken ovabulmin emulsified in injected alum on days 0 and 14, then were challenged with an aerosol generated from 1% ovabulmin on days 24, 25 and 26. Tripterygium poiyglycosid was injected intraperitoneally at 50 mg/kg on days 25, 26 and 27 after ovabulmin challenge. Dexamethasone was administrated to mice at 2 mg/kg on day 21, 23 before ovabulmin challenge. The airway hyperresponsiveness, mucus production, eosinophils in parabronchial area and bronchoalveolar lavage fluid and the level of interleukin-5, granulo-macrophage clone stimulating factor in bronchoalveolar lavage fluid were measured as indexes of inflammation. Results： Tripterygium polyglycosid treatment after ovabulmin challenge completely inhibited eosinophil infiltration in bronchoalveolar lavage fluid [（0.63 ± 0.34） × 10^4 vs. （75.0± 14.8） × 10^4, P〈0.05] and the peribrochial area （12.60 ± 3.48 mm^2 vs. 379.0 ± 119.3 mm^2, P〈0.05）, mucus overproduction in airway （2.8± 1.7 vs. 7.1± 5.6, P〈0.05）, and increased interleukin-5 levels in bronchoalveolar lavage fluid （28.8±2.8 pg/mL vs. 7.5± 3.5 pg/mL, P〈0.05）. Meanwhile, Tripterygium polyglycosid treatment after ovabulmin challenge also partially inhibited airway hyperresponsiveness. The level of granulo-macrophage clone stimulating factor in bronchoalveolar lavage fluid didn＇t change with drugs intervention. Conclusions： The administration of Tripterygium polyglycosid could inhibit the established airway inflammation and reduce the airway hyperresponsiveness of allergic asthmatic mice. It provides a possible altemative therapeutic for asthma.展开更多
文摘Objective: To observe the effect and adverse reaction of tripterygium polyglycosides (TP) combined with small dose of methotrexate (MTX) in treating senile rheumatoid arthritis (RA). Methods: Sixty old patients with RA were equally divided into two groups, the treated group was treated with oral intake of TP (10 mg three times per day) plus MTX (7.5 mg, once per week), and the control group was treated with MTX (15 mg per week) respectively for 3 months. The changes from before to after treatment in resting pain, morning rigidity, bilateral grip strength, local tenderness index, arthroncus index, patient's evaluation, doctor's evaluation, erythrocyte sedimentation rate (ESR), C-reaction protein (CRP) and rheumatoid factor(RF) titre were observed and the clinical therapeutic effect assessed. Results: The total effective rate in the treated group was 86.67% and that in the control group 83.33% , showing insignificant difference between them (P>0.05). But the improvement in local tenderness index, arthroncus index, morning rigidity and ESR in the treated group was significantly better than those in the control group (P<0.01 or P<0.05). The occurrence rate of adverse reaction in the treated group was 26. 67% , which was lower than that in the control group (43.33%), showing significant difference (P<0.01). Conclusion: TP combined with small dose of MTX is a favorable therapeutic method for treating senile RA.
基金Supported by the National Natural Science Foundation of China (No.30600266)
文摘Objective： To investigate the effect of Tripterygium polyglycosid on establishing airway eosinophil infiltration and related airway hyperresponsiveness of asthmatic mice. Methods： A mature murine asthmatic model was made with ovabulmin sensitized and challenged C57BL/6 mice. Forty mice were divided into four groups with 10 mice in each group： mice sensitized and challenged with saline （WS group）, mice sensitized and challenged with ovalbumin （WO group）, mice sensitized and challenged with ovalbumin and treated with Tripterygium polyglycosid （TP group） and Dexamethasone （DXM group）. The mice were intraperitoneally injected with 20 I^g chicken ovabulmin emulsified in injected alum on days 0 and 14, then were challenged with an aerosol generated from 1% ovabulmin on days 24, 25 and 26. Tripterygium poiyglycosid was injected intraperitoneally at 50 mg/kg on days 25, 26 and 27 after ovabulmin challenge. Dexamethasone was administrated to mice at 2 mg/kg on day 21, 23 before ovabulmin challenge. The airway hyperresponsiveness, mucus production, eosinophils in parabronchial area and bronchoalveolar lavage fluid and the level of interleukin-5, granulo-macrophage clone stimulating factor in bronchoalveolar lavage fluid were measured as indexes of inflammation. Results： Tripterygium polyglycosid treatment after ovabulmin challenge completely inhibited eosinophil infiltration in bronchoalveolar lavage fluid [（0.63 ± 0.34） × 10^4 vs. （75.0± 14.8） × 10^4, P〈0.05] and the peribrochial area （12.60 ± 3.48 mm^2 vs. 379.0 ± 119.3 mm^2, P〈0.05）, mucus overproduction in airway （2.8± 1.7 vs. 7.1± 5.6, P〈0.05）, and increased interleukin-5 levels in bronchoalveolar lavage fluid （28.8±2.8 pg/mL vs. 7.5± 3.5 pg/mL, P〈0.05）. Meanwhile, Tripterygium polyglycosid treatment after ovabulmin challenge also partially inhibited airway hyperresponsiveness. The level of granulo-macrophage clone stimulating factor in bronchoalveolar lavage fluid didn＇t change with drugs intervention. Conclusions： The administration of Tripterygium polyglycosid could inhibit the established airway inflammation and reduce the airway hyperresponsiveness of allergic asthmatic mice. It provides a possible altemative therapeutic for asthma.
