In this study, we aimed to explore the role of ursolic acid in the neural regeneration of the injured sciatic nerve. BALB/c mice were used to establish models of sciatic nerve injury through unilateral sciatic nerve c...In this study, we aimed to explore the role of ursolic acid in the neural regeneration of the injured sciatic nerve. BALB/c mice were used to establish models of sciatic nerve injury through unilateral sciatic nerve complete transection and microscopic anastomosis at 0.5 cm below the ischial tuber-osity. The successful y generated model mice were treated with 10, 5, or 2.5 mg/kg ursolic acid via intraperitoneal injection. Enzyme-linked immunosorbent assay results showed that serum S100 protein expression level gradual y increased at 1-4 weeks after sciatic nerve injury, and significantly decreased at 8 weeks. As such, ursolic acid has the capacity to significantly increase S100 protein expression levels. Real-time quantitative PCR showed that S100 mRNA expression in the L 4-6 segments on the injury side was increased after ursolic acid treatment. In addition, the muscular mass index in the soleus muscle was also increased in mice treated with ursolic acid. Toluidine blue staining revealed that the quantity and average diameter of myelinated nerve fibers in the injured sciatic nerve were significantly increased after treatment with ursolic acid. 10 and 5 mg/kg of ursolic acid produced stronger effects than 2.5 mg/kg of ursolic acid. Our findings indicate that ursolic acid can dose-dependently increase S100 expression and promote neural regeneration in BALB/c mice fol owing sciatic nerve injury.展开更多
A new compound, lepida acid A 1, has been isolated from the fruiting bodies of Basidiomycetes Russula lepida. Its structure was elucidated by spectral methods.
A series of natural product 23-hydroxybetulinic acid derivatives were prepared. In the preparation of mono-O-benzoyl ester derivative, it was observed that benzoyl group migrated from 3-0- to 23-O-position during the ...A series of natural product 23-hydroxybetulinic acid derivatives were prepared. In the preparation of mono-O-benzoyl ester derivative, it was observed that benzoyl group migrated from 3-0- to 23-O-position during the detritylation.展开更多
Objective: To determine whether corosolic acid(CA) targeting nuclear protein expression of Nrf2 activation can be used to attenuate renal damage and preserve renal function in alloxan diabetic mice.Methods: A mouse mo...Objective: To determine whether corosolic acid(CA) targeting nuclear protein expression of Nrf2 activation can be used to attenuate renal damage and preserve renal function in alloxan diabetic mice.Methods: A mouse model with diabetic nephropathy was established to examine the Nrf2 expression. Mice were randomly divided into control, diabetic control, and CA groups treated at 0.4 mg/kg, 2 mg/kg and 10 mg/kg p.o. for 8 weeks. Diabetes was induced in mice by single intraperitoneal injection of alloxan 200 mg/kg in all groups except the control. The mice with fasting blood glucose level over 200 mg/d L were considered as diabetic and were employed in the study. After 4 th and 8 th weeks, urine samples were collected(using metabolic cages) to measure protein and urea. Animals were euthanized, and serum samples were collected to estimate the glucose, creatinine, total protein, urea and blood urea nitrogen. Kidney was isolated at the end of experiment for histology to evaluate anti-oxidant parameters. Immunohistochemistry was performed to examine the Nrf2 expression.Results: CA treatment showed dose dependent reduction in level of biochemical parameters in serum and urine. CA group(10 mg/kg) showed significantly higher body weight and reduced kidney weight. Histopathological examination revealed reduced inflammation, collagen deposition and glomerulosclerosis in renal tissue. CA attenuated renal dysfunction, oxidative stress and inflammatory pro-cytokine levels.Conclusions: CA treatment exhibited ameliorative effect on kidney in mice with its enhanced Nrf2 expression.展开更多
A new oleanane-type triterpene was isolated from the roots of Doellingeria scaber. Its structure was identified as 3-oxo-16a-hydroxy-olean-12-en-28-oic acid based on 1D and 2D NMR spectroscopy and X-ray analysis.
A new ursane-type triterpene, 3 beta-hydroxy-urs-5(6)-en-28-oic acid 1, was isolated from the aerial parts of Teucrium integrifolium and characterized. The structure of this new compound was mainly established by 2D N...A new ursane-type triterpene, 3 beta-hydroxy-urs-5(6)-en-28-oic acid 1, was isolated from the aerial parts of Teucrium integrifolium and characterized. The structure of this new compound was mainly established by 2D NMR techniques (COSY, HETCOR, HMBC).展开更多
为研究长叶香茶菜Rabdosia stracheyi(Benth ex Hook.)Hara的化学成分,本文采用硅胶、反相硅胶C 18、葡聚糖凝胶Sephadex LH-20、反相半制备高效液相等色谱方法对长叶香茶菜地上部分进行分离、纯化,利用1 H NMR、13 C NMR、HR-ESI-MS等...为研究长叶香茶菜Rabdosia stracheyi(Benth ex Hook.)Hara的化学成分,本文采用硅胶、反相硅胶C 18、葡聚糖凝胶Sephadex LH-20、反相半制备高效液相等色谱方法对长叶香茶菜地上部分进行分离、纯化,利用1 H NMR、13 C NMR、HR-ESI-MS等波谱数据,结合参考文献,鉴定化合物的结构。结果表明,从长叶香茶菜地上部分分离得到12个三萜类化合物,它们分别是木栓醇(1)、齐墩果酸(2)、3β-羟基-齐墩果烷-11,13(18)-二烯-28酸(3)、2α,3α-二羟基齐墩果-12-烯-28酸(4)、2 a,3a,24-三羟基-12-烯-28-齐墩果酸(5)、2α,3β,24-三羟基齐墩果-12-烯-28酸(6)、cleistocalyxin(7)、熊果醇(8)、2α-羟基乌苏酸(9)、2a,3a,24-三羟基熊果-12,20(30)-二烯-28-酸甲酯(10)、maquatic acid(11)和2α,3α,24-三羟基-12,20(30)-二烯乌苏酸(12)。以上得到的三萜类化合物均为首次从该植物中分离得到。展开更多
基金financially sponsored by the Science and Technology Ministry of Jilin Province,No.200705318
文摘In this study, we aimed to explore the role of ursolic acid in the neural regeneration of the injured sciatic nerve. BALB/c mice were used to establish models of sciatic nerve injury through unilateral sciatic nerve complete transection and microscopic anastomosis at 0.5 cm below the ischial tuber-osity. The successful y generated model mice were treated with 10, 5, or 2.5 mg/kg ursolic acid via intraperitoneal injection. Enzyme-linked immunosorbent assay results showed that serum S100 protein expression level gradual y increased at 1-4 weeks after sciatic nerve injury, and significantly decreased at 8 weeks. As such, ursolic acid has the capacity to significantly increase S100 protein expression levels. Real-time quantitative PCR showed that S100 mRNA expression in the L 4-6 segments on the injury side was increased after ursolic acid treatment. In addition, the muscular mass index in the soleus muscle was also increased in mice treated with ursolic acid. Toluidine blue staining revealed that the quantity and average diameter of myelinated nerve fibers in the injured sciatic nerve were significantly increased after treatment with ursolic acid. 10 and 5 mg/kg of ursolic acid produced stronger effects than 2.5 mg/kg of ursolic acid. Our findings indicate that ursolic acid can dose-dependently increase S100 expression and promote neural regeneration in BALB/c mice fol owing sciatic nerve injury.
文摘A new compound, lepida acid A 1, has been isolated from the fruiting bodies of Basidiomycetes Russula lepida. Its structure was elucidated by spectral methods.
基金supported by the National Natural Science Foundation of the People's Republic of China(No.30472083).
文摘A series of natural product 23-hydroxybetulinic acid derivatives were prepared. In the preparation of mono-O-benzoyl ester derivative, it was observed that benzoyl group migrated from 3-0- to 23-O-position during the detritylation.
基金the financial support received under University Grants Commission Minor Research Scheme with File no. 47-831/13(WRO), Pune
文摘Objective: To determine whether corosolic acid(CA) targeting nuclear protein expression of Nrf2 activation can be used to attenuate renal damage and preserve renal function in alloxan diabetic mice.Methods: A mouse model with diabetic nephropathy was established to examine the Nrf2 expression. Mice were randomly divided into control, diabetic control, and CA groups treated at 0.4 mg/kg, 2 mg/kg and 10 mg/kg p.o. for 8 weeks. Diabetes was induced in mice by single intraperitoneal injection of alloxan 200 mg/kg in all groups except the control. The mice with fasting blood glucose level over 200 mg/d L were considered as diabetic and were employed in the study. After 4 th and 8 th weeks, urine samples were collected(using metabolic cages) to measure protein and urea. Animals were euthanized, and serum samples were collected to estimate the glucose, creatinine, total protein, urea and blood urea nitrogen. Kidney was isolated at the end of experiment for histology to evaluate anti-oxidant parameters. Immunohistochemistry was performed to examine the Nrf2 expression.Results: CA treatment showed dose dependent reduction in level of biochemical parameters in serum and urine. CA group(10 mg/kg) showed significantly higher body weight and reduced kidney weight. Histopathological examination revealed reduced inflammation, collagen deposition and glomerulosclerosis in renal tissue. CA attenuated renal dysfunction, oxidative stress and inflammatory pro-cytokine levels.Conclusions: CA treatment exhibited ameliorative effect on kidney in mice with its enhanced Nrf2 expression.
文摘A new oleanane-type triterpene was isolated from the roots of Doellingeria scaber. Its structure was identified as 3-oxo-16a-hydroxy-olean-12-en-28-oic acid based on 1D and 2D NMR spectroscopy and X-ray analysis.
文摘A new ursane-type triterpene, 3 beta-hydroxy-urs-5(6)-en-28-oic acid 1, was isolated from the aerial parts of Teucrium integrifolium and characterized. The structure of this new compound was mainly established by 2D NMR techniques (COSY, HETCOR, HMBC).
文摘为研究长叶香茶菜Rabdosia stracheyi(Benth ex Hook.)Hara的化学成分,本文采用硅胶、反相硅胶C 18、葡聚糖凝胶Sephadex LH-20、反相半制备高效液相等色谱方法对长叶香茶菜地上部分进行分离、纯化,利用1 H NMR、13 C NMR、HR-ESI-MS等波谱数据,结合参考文献,鉴定化合物的结构。结果表明,从长叶香茶菜地上部分分离得到12个三萜类化合物,它们分别是木栓醇(1)、齐墩果酸(2)、3β-羟基-齐墩果烷-11,13(18)-二烯-28酸(3)、2α,3α-二羟基齐墩果-12-烯-28酸(4)、2 a,3a,24-三羟基-12-烯-28-齐墩果酸(5)、2α,3β,24-三羟基齐墩果-12-烯-28酸(6)、cleistocalyxin(7)、熊果醇(8)、2α-羟基乌苏酸(9)、2a,3a,24-三羟基熊果-12,20(30)-二烯-28-酸甲酯(10)、maquatic acid(11)和2α,3α,24-三羟基-12,20(30)-二烯乌苏酸(12)。以上得到的三萜类化合物均为首次从该植物中分离得到。
