To analyze the genetic differentiation of the host- and geo-populations of Liriomyza sativae Blanchard, the β-tubulin gene of 5 host-populations and 6 geo-populations of L. sativae was sequenced. Subsequently, the se...To analyze the genetic differentiation of the host- and geo-populations of Liriomyza sativae Blanchard, the β-tubulin gene of 5 host-populations and 6 geo-populations of L. sativae was sequenced. Subsequently, the sequences were analyzed by biosoftware DNAStar and MEGA, and the phylogenetic trees constructed. The results obtained by the two softwares were similar, that is, the sequences of β-tubulin gene were more than 98% homologous, among the host- and geo-populations of L. sativae, with only 8 variable sites, but no insertions and deletions were detected. It seems that differentiations in β-tubulin gene among the host- and geo-populations of L. sativae are related to the hobby to hosts and the geographical distributions, respectively.展开更多
为了给筛选萼脊兰内参基因和研究 TUB 基因的生理功能提供研究基础，对萼脊兰 TUB 基因片段进行克隆与序列分析。采用 CTAB 法提取萼脊兰盛花期花瓣总 RNA，并运用 RT-PCR 方法克隆萼脊兰 TUB 基因序列，长度为1055 bp，编码351个氨基...为了给筛选萼脊兰内参基因和研究 TUB 基因的生理功能提供研究基础，对萼脊兰 TUB 基因片段进行克隆与序列分析。采用 CTAB 法提取萼脊兰盛花期花瓣总 RNA，并运用 RT-PCR 方法克隆萼脊兰 TUB 基因序列，长度为1055 bp，编码351个氨基酸残基，氨基酸序列中存在微管蛋白的保守区域 GGGTGSG。序列分析结果表明：与其他已登录物种的 TUB 基因相比，其核苷酸序列的同源性达75％～96％，与朵丽蝶兰（JN185665．1）同源性高达96％，氨基酸序列的同源性也在96％以上，且与单子叶植物的同源序列表现出较高的相似性。在 GenBank 注册，登录号为KP686397。展开更多
为筛选紫玉兰‘红元宝’(Magnolia liliflora‘Hongyuanbao’)二次花芽分化阶段稳定表达的内参基因,该研究以‘红元宝’不同花芽分化时期的花芽和叶为材料,基于转录组数据,筛选出8个候选内参基因,即泛素酶基因(UBC)、肌动蛋白(ACT)、微...为筛选紫玉兰‘红元宝’(Magnolia liliflora‘Hongyuanbao’)二次花芽分化阶段稳定表达的内参基因,该研究以‘红元宝’不同花芽分化时期的花芽和叶为材料,基于转录组数据,筛选出8个候选内参基因,即泛素酶基因(UBC)、肌动蛋白(ACT)、微管蛋白β链(β-TUB)、微管蛋白β-5链(β-TUB5)、微管蛋白α-3链(α-TUB3)、磷酸烯醇丙酮酸羧化酶(PEPC)、酰基载体蛋白2(ACP2)、酰基载体蛋白3(ACP3)。运用Primer Premier 5设计引物,简单克隆和熔解曲线验证引物特异性;利用qRT-PCR技术检测各个候选内参基因的表达情况,结合GeNorm、NormFinder、BestKeeper软件和RefFinder在线工具综合评估其表达稳定性,并通过目的基因TFL1的表达分析验证其可靠性。结果表明:(1)8个候选内参基因条带位置正确,熔解曲线呈单一峰,说明引物特异性良好。(2)β-TUB、β-TUB5和α-TUB3是‘红元宝’不同花芽分化时期较为稳定的内参基因,而UBC和ACT为稳定性最低的内参基因。(3)β-TUB5、α-TUB3、β-TUB及其组合的相对表达量趋于一致,而ACT和UBC并未对目的基因的表达量进行有效的标准化。因此,β-TUB、β-TUB5和α-TUB3可作为‘红元宝’二次花芽分化研究中稳定表达的内参基因。该研究结果将为木兰属植物二次成花分子调控机制研究提供依据。展开更多
Scar formation after spinal cord injury is regarded as an obstacle to axonal regeneration and functional recovery.Epothilone B provides moderate microtubule stabilization and is mainly used for anti-tumor therapy.It a...Scar formation after spinal cord injury is regarded as an obstacle to axonal regeneration and functional recovery.Epothilone B provides moderate microtubule stabilization and is mainly used for anti-tumor therapy.It also reduces scar tissue formation and promotes axonal regeneration after spinal cord injury.The aim of the present study was to investigate the effect and mechanism of the microtubule-stabilizing reagent epothilone B in decreasing fibrotic scarring through its action on pericytes after spinal cord injury.A rat model of spinal cord injury was established via dorsal complete transection at the T10 vertebra.The rats received an intraperitoneal injection of epothilone B（0.75 mg/kg） at 1 and 15 days post-injury in the epothilone B group or normal saline in the vehicle group.Neuron-glial antigen 2,platelet-derived growth factor receptor β,and fibronectin protein expression were dramatically lower in the epothilone B group than in the vehicle group,but β-tubulin protein expression was greater.Glial fibrillary acidic protein at the injury site was not affected by epothilone B treatment.The Basso,Beattie,and Bresnahan locomotor scores were significantly higher in the epothilone B group than in the vehicle group.The results of this study demonstrated that epothilone B reduced the number of pericytes,inhibited extracellular matrix formation,and suppressed scar formation after spinal cord injury.展开更多
Interest on the genus Camelina has recently increased due to the biofuel, or jet fuel, potential of the oil extracted from seeds of the cultivated species Camelina sativa (L.) Crantz. While our knowledge on C. sativa ...Interest on the genus Camelina has recently increased due to the biofuel, or jet fuel, potential of the oil extracted from seeds of the cultivated species Camelina sativa (L.) Crantz. While our knowledge on C. sativa is constantly augmenting, only few studies have been performed on the other species of the genus, which could be a potentially useful material for the genetic improvement of C. sativa. The genus Camelina consists of 11 species, but only six (C. sativa, C. microcarpa, C. alyssum, C. rumelica, C. hispida and C. laxa) could be retrieved from germplasm banks to carry out genomic fingerprinting studies based on the use of the cTBP molecular marker. Each species, with the exception of C. alyssum that is proposed to be a subspecies of C. sativa, shows a distinct cTBP profile resulting from multiple DNA length polymorphisms present in the second intron of the members of the β-tubulin gene family. In contrast to the high level of genetic diversity detected among the six Camelina species, low variability is observed among and within the accessions of the same species, except for C. hispida that is characterized by an intra-accession high number of cTBP polymorphic bands. In addition, cTBP is also able to identify incorrectly classified accessions and provide information on the ploidy level of each species.展开更多
基金funded by National Natural Science Foundation of China(30370932)973 Project of China(2006CB102002).
文摘To analyze the genetic differentiation of the host- and geo-populations of Liriomyza sativae Blanchard, the β-tubulin gene of 5 host-populations and 6 geo-populations of L. sativae was sequenced. Subsequently, the sequences were analyzed by biosoftware DNAStar and MEGA, and the phylogenetic trees constructed. The results obtained by the two softwares were similar, that is, the sequences of β-tubulin gene were more than 98% homologous, among the host- and geo-populations of L. sativae, with only 8 variable sites, but no insertions and deletions were detected. It seems that differentiations in β-tubulin gene among the host- and geo-populations of L. sativae are related to the hobby to hosts and the geographical distributions, respectively.
文摘为了给筛选萼脊兰内参基因和研究 TUB 基因的生理功能提供研究基础，对萼脊兰 TUB 基因片段进行克隆与序列分析。采用 CTAB 法提取萼脊兰盛花期花瓣总 RNA，并运用 RT-PCR 方法克隆萼脊兰 TUB 基因序列，长度为1055 bp，编码351个氨基酸残基，氨基酸序列中存在微管蛋白的保守区域 GGGTGSG。序列分析结果表明：与其他已登录物种的 TUB 基因相比，其核苷酸序列的同源性达75％～96％，与朵丽蝶兰（JN185665．1）同源性高达96％，氨基酸序列的同源性也在96％以上，且与单子叶植物的同源序列表现出较高的相似性。在 GenBank 注册，登录号为KP686397。
文摘为筛选紫玉兰‘红元宝’(Magnolia liliflora‘Hongyuanbao’)二次花芽分化阶段稳定表达的内参基因,该研究以‘红元宝’不同花芽分化时期的花芽和叶为材料,基于转录组数据,筛选出8个候选内参基因,即泛素酶基因(UBC)、肌动蛋白(ACT)、微管蛋白β链(β-TUB)、微管蛋白β-5链(β-TUB5)、微管蛋白α-3链(α-TUB3)、磷酸烯醇丙酮酸羧化酶(PEPC)、酰基载体蛋白2(ACP2)、酰基载体蛋白3(ACP3)。运用Primer Premier 5设计引物,简单克隆和熔解曲线验证引物特异性;利用qRT-PCR技术检测各个候选内参基因的表达情况,结合GeNorm、NormFinder、BestKeeper软件和RefFinder在线工具综合评估其表达稳定性,并通过目的基因TFL1的表达分析验证其可靠性。结果表明:(1)8个候选内参基因条带位置正确,熔解曲线呈单一峰,说明引物特异性良好。(2)β-TUB、β-TUB5和α-TUB3是‘红元宝’不同花芽分化时期较为稳定的内参基因,而UBC和ACT为稳定性最低的内参基因。(3)β-TUB5、α-TUB3、β-TUB及其组合的相对表达量趋于一致,而ACT和UBC并未对目的基因的表达量进行有效的标准化。因此,β-TUB、β-TUB5和α-TUB3可作为‘红元宝’二次花芽分化研究中稳定表达的内参基因。该研究结果将为木兰属植物二次成花分子调控机制研究提供依据。
基金supported by a grant from the Science and Technology Developing Program of Shandong Provincial Government of China,No.2010GSF10254a grant from the Medical and Health Science and Technology Plan Project of Shandong Province of China,No.2015WS0504
文摘Scar formation after spinal cord injury is regarded as an obstacle to axonal regeneration and functional recovery.Epothilone B provides moderate microtubule stabilization and is mainly used for anti-tumor therapy.It also reduces scar tissue formation and promotes axonal regeneration after spinal cord injury.The aim of the present study was to investigate the effect and mechanism of the microtubule-stabilizing reagent epothilone B in decreasing fibrotic scarring through its action on pericytes after spinal cord injury.A rat model of spinal cord injury was established via dorsal complete transection at the T10 vertebra.The rats received an intraperitoneal injection of epothilone B（0.75 mg/kg） at 1 and 15 days post-injury in the epothilone B group or normal saline in the vehicle group.Neuron-glial antigen 2,platelet-derived growth factor receptor β,and fibronectin protein expression were dramatically lower in the epothilone B group than in the vehicle group,but β-tubulin protein expression was greater.Glial fibrillary acidic protein at the injury site was not affected by epothilone B treatment.The Basso,Beattie,and Bresnahan locomotor scores were significantly higher in the epothilone B group than in the vehicle group.The results of this study demonstrated that epothilone B reduced the number of pericytes,inhibited extracellular matrix formation,and suppressed scar formation after spinal cord injury.
文摘Interest on the genus Camelina has recently increased due to the biofuel, or jet fuel, potential of the oil extracted from seeds of the cultivated species Camelina sativa (L.) Crantz. While our knowledge on C. sativa is constantly augmenting, only few studies have been performed on the other species of the genus, which could be a potentially useful material for the genetic improvement of C. sativa. The genus Camelina consists of 11 species, but only six (C. sativa, C. microcarpa, C. alyssum, C. rumelica, C. hispida and C. laxa) could be retrieved from germplasm banks to carry out genomic fingerprinting studies based on the use of the cTBP molecular marker. Each species, with the exception of C. alyssum that is proposed to be a subspecies of C. sativa, shows a distinct cTBP profile resulting from multiple DNA length polymorphisms present in the second intron of the members of the β-tubulin gene family. In contrast to the high level of genetic diversity detected among the six Camelina species, low variability is observed among and within the accessions of the same species, except for C. hispida that is characterized by an intra-accession high number of cTBP polymorphic bands. In addition, cTBP is also able to identify incorrectly classified accessions and provide information on the ploidy level of each species.
