Taking tumorous stem mustard infected by black spot disease as the research material, the ribosomal 5.8S rDNA and its flanking ITS region were cloned, sequenced and aligned in the study. The results showed that the ba...Taking tumorous stem mustard infected by black spot disease as the research material, the ribosomal 5.8S rDNA and its flanking ITS region were cloned, sequenced and aligned in the study. The results showed that the base sequence of pathogens collected from five different sites was almost identical with that of Alternaria brassicae, and the similarity degree reached 99.68%, without base difference greater than 3 bp. It had obvious differences with the sequences of A. brassicicola and A. japonica, and the base difference was greater than 3 bp with a lot of fragment deletions. It was preliminarily determined that the pathogen cau- sing black spot disease on tumorous stem mustard was A. brassicae.展开更多
Objective: To explore the role of miR-448 in regulating MAGEA6/AMPK signaling pathway in the biological study of hepatocellular carcinoma (HCC) tumor stem cells. Methods: Using the database, the hepatocellular carcino...Objective: To explore the role of miR-448 in regulating MAGEA6/AMPK signaling pathway in the biological study of hepatocellular carcinoma (HCC) tumor stem cells. Methods: Using the database, the hepatocellular carcinoma related expression chips were obtained and the regulatory mirnas of candidate genes were predicted, and the predicted results were analyzed. The effects of miR-448 and MAGEA6 on the pellet formation rate and clone formation rate of hepatocellular carcinoma stem cells were detected by immunofluorescence identification of stem cell markers and light microscope counting method. The effects of miR-448 and MAGEA6 on migration and invasion of hepatocellular carcinoma stem cells were detected by scratch and Transwell assay. Dual luciferase reporter assay to verify whether miR-448 targets MAGEA6. The expression and influence of miR-448 on MAGEA6 and AMPK pathway were detected by qRT-PCR and Western blot. Results: It was found that miR-448 may directly regulate the expression of MAGEA6. Overexpression of miR-448 inhibited the characteristics, proliferation, migration, and invasion of hepatocellular carcinoma stem cells in vitro, as well as the ability of xenograft tumor formation in vivo. However, inhibition of miR-448 showed opposite results. In addition, miR-448 directly targets MAGEA6 and regulates AMPK signaling. Silencing MAGEA6 and adding AMPK activator further verified that miR-448 activated AMPK signaling pathway by targeting MAGEA6, thus affecting characteristics, proliferation, migration and invasion of hepatoma stem cells. Conclusions: Our results reveal that miR-448 activates AMPK signaling pathway by targeting MAGEA6, thereby affecting characteristics, proliferation, migration and invasion of hepatoma stem cells. It is suggested that overexpression of miR-448 may be a new therapeutic strategy for hepatocellular carcinoma.展开更多
Cancer cells possess metabolic properties that are different from those of benign cells.p21,encoded by CDKN1A gene,also named p21Cip1/WAF1,was first identified as a cyclin-dependent kinase regulator that suppresses ce...Cancer cells possess metabolic properties that are different from those of benign cells.p21,encoded by CDKN1A gene,also named p21Cip1/WAF1,was first identified as a cyclin-dependent kinase regulator that suppresses cell cycle G1/S phase and retinoblastoma protein phosphorylation.CDKN1A(p21)acts as the downstream target gene of TP53(p53),and its expression is induced by wild-type p53 and it is not associated with mutant p53.p21 has been characterized as a vital regulator that involves multiple cell functions,including G1/S cell cycle progression,cell growth,DNA damage,and cell stemness.In 1994,p21 was found as a tumor suppressor in brain,lung and colon cancer by targeting p53 and was associated with tumorigenesis and metastasis.Notably,p21 plays a significant role in tumor development through p53-dependent and p53-independent pathways.In addition,expression of p21 is closely related to the resting state or terminal differentiation of cells.p21 is also associated with cancer stem cells and acts as a biomarker for such cells.In cancer therapy,given the importance of p21 in regulating the G1/S and G2 check points,it is not surprising that p21 is implicated in response to many cancer treatments and p21 promotes the effect of oncolytic virotherapy.展开更多
Although it is believed that glioma is derived from brain tumor stem cells, the source and molecular signal pathways of these cells are still unclear. In this study, we used stable doxycycline-inducible transgenic mou...Although it is believed that glioma is derived from brain tumor stem cells, the source and molecular signal pathways of these cells are still unclear. In this study, we used stable doxycycline-inducible transgenic mouse brain tumor models (c-myc/SV40Tag+/Tet-on+) to explore the malignant trans- formation potential of neural stem cells by observing the differences of neural stem cells and brain tumor stem cells in the tumor models. Results showed that chromosome instability occurred in brain tumor stem cells. The numbers of cytolysosomes and autophagosomes in brain tumor stem cells and induced neural stem cells were lower and the proliferative activity was obviously stronger than that in normal neural stem cells. Normal neural stem cells could differentiate into glial fibrillary acidic protein-positive and microtubule associated protein-2-positive cells, which were also negative for nestin. However, glial fibrillary acidic protein/nestin, microtubule associated protein-2/nestin, and glial fibrillary acidic protein/microtubule associated protein-2 double-positive cells were found in induced neural stem cells and brain tumor stem cells. Results indicate that induced neural stem cells are similar to brain tumor stem cells, and are possibly the source of brain tumor stem cells.展开更多
Extensive in vitro and in vivo research reveals multiple intracellular molecular targets of Antrodia camphorata,and these targets affect growth,apoptosis,angiogenesis,invasion and metastasis of cells.These targets inc...Extensive in vitro and in vivo research reveals multiple intracellular molecular targets of Antrodia camphorata,and these targets affect growth,apoptosis,angiogenesis,invasion and metastasis of cells.These targets include tumor suppressor,cell cycle regulator,transcription factor,angiogenesis and metastasis factor,apoptosis and survival regulator,etc.Additionally,more and more attention has been paid to the molecular mechanism of A.camphorata on the regulation of tumor stem cells.Meanwhile,there is evidence that the immunoregulation of A.camphorata is enhanced,which may lead cell cycle arrest or apoptosis.In this paper,molecular mechanism of tumor cells and tumor stem cells regulated at multiple targets by A.camphorata in vitro and in vivo in the past decade is summarized.展开更多
Objective To investigate the effect of dendritic cells pulsed with brain tumor stem cells which are used to treat on intracranial glioma. Methods We obtained murine brain tumor stem cells by grow ing C6 cells in epide...Objective To investigate the effect of dendritic cells pulsed with brain tumor stem cells which are used to treat on intracranial glioma. Methods We obtained murine brain tumor stem cells by grow ing C6 cells in epidermal grow th factor/basic fibroblast grow th factor w ithout serum.Dendritic cells isolated from rat bone marrow w ere pulsed w ith BTSCs. Rat brain展开更多
BACKGROUND:Emerging evidence suggests that pancreatic adenocarcinoma is hierarchically organized and sustained by pancreatic cancer stem cells.Furthermore,elimination of these cells is possible and therapeutically rel...BACKGROUND:Emerging evidence suggests that pancreatic adenocarcinoma is hierarchically organized and sustained by pancreatic cancer stem cells.Furthermore,elimination of these cells is possible and therapeutically relevant.This study aimed to investigate the expression patterns of pancreatic cancer stem cell surface markers CD44,CD24 and ESA in pancreatic adenocarcinoma cell lines and explore the influence of their local microenvironment.METHODS:Flow cytometry was used to analyze the expression patterns of CD44,CD24 and ESA in five pancreatic adenocarcinoma cell lines (PANC-1,PC-2,MIA-Paca-2,AsPC-1 and BxPC-3).In addition,the capacity for sphereformation in serum-free medium of four cell lines (PANC-1,PC-2,MIA-Paca-2 and BxPC-3) was assessed.Then,the same assays were performed when tumor cell spheres were developed.The role of sonic hedgehog (SHH) in cell spheres from PANC-1 and MIA-Paca-2 were also assessed by RT-PCR.RESULTS:CD44 and CD24 were detected in PANC-1.Only CD44 expression was detected in PC-2,MIA-Paca-2 and AsPC-1.CD44,CD24 and ESA were all detected in BxPC-3.Tumor cell spheres developed in PANC-1 and MIA-Paca-2 in serumfree medium.This was accompanied by an increase in CD24 expression and a decrease in CD44 expression in PANC-1.Interestingly,the expression of CD44 and CD24 returned to initial levels once the medium was changed back from serumfree to serum-containing medium.No significant change in the expression of CD44 was detected in MIA-Paca-2.Furthermore,the relative quantification of SHH mRNA in PANC-1 cell spheres was significantly higher than that in cells cultured in the serum-containing medium.CONCLUSION:The expression patterns of the pancreatic cancer stem cell surface markers CD44,CD24 and ESA were diverse in different pancreatic adenocarcinoma cell lines and changed with their local microenvironment.展开更多
Background:We have shown that downregulation of materally expressed gene 3(MEG3)promoted autophagy and epithelial-mesenchymal transition to promote neuroblastoma(NB)progression.In present study,we aim to further disco...Background:We have shown that downregulation of materally expressed gene 3(MEG3)promoted autophagy and epithelial-mesenchymal transition to promote neuroblastoma(NB)progression.In present study,we aim to further discover the role of MEG3 in NB.Methods:Expression levels of MEG3 in stabled transfected cells were detected by qPCR.Flow cytometry was used to examine the effects of MEG3 overexpression on apoptosis and cell cycle.Moreover,tumor stemness was detected by tumor sphere formation assay.Results:QPCR showed that MEG3 was successfully overexpressed in SK-N-BE(2)C and SK-N-AS cells.CCK8 indicated MEG3 reduced cell proliferation in two NB cells.Flow cytometry revealed that MEG3 promoted apoptosis and caused G0/G1 arrest.Using chromatin isolation by RNA purification(ChIRP)and tumor sphere formation assays,we revealed that upregulation of MEG3 decreased tumor stemness by decreasing Nestin transcription.Conclusions:Overall,present study confirmed an anti-cancer role of MEG3 in NB,demonstrated that MEG3 could be a potential therapeutic target of NB.展开更多
Adhesion molecules mediate cell-to-cell and cell-to-extracellular matrix interactions and transmit mechanical and chemical signals among them.Various mechanisms deregulate adhesion molecules in cancer,enabling tumor c...Adhesion molecules mediate cell-to-cell and cell-to-extracellular matrix interactions and transmit mechanical and chemical signals among them.Various mechanisms deregulate adhesion molecules in cancer,enabling tumor cells to proliferate without restraint,invade through tissue boundaries,escape from immune surveillance,and survive in the tumor microenvironment.Recent studies have revealed that adhesion molecules also drive angiogenesis,reshape metabolism,and are involved in stem cell self-renewal.In this review,we summarize the functions and mechanisms of adhesion molecules in cancer and the tumor microenvironment,as well as the therapeutic strategies targeting adhesion molecules.These studies have implications for furthering our understanding of adhesion molecules in cancer and providing a paradigm for exploring novel therapeutic approaches.展开更多
Background Acute lung injury (ALl) and end-stage acute respiratory distress syndrome (ARDS) were among the most common causes of death in intensive care units. The activation of an inflammatory response and the da...Background Acute lung injury (ALl) and end-stage acute respiratory distress syndrome (ARDS) were among the most common causes of death in intensive care units. The activation of an inflammatory response and the damage of pulmonary epithelium and endotheliumwerethe hallmark of ALI/ARDS. Recent studies had demonstrated the importance of mesenchymal stem cells (MSCs) in maintaining the normal pulmonary endothelial and epithelial function as well as participating in modulating the inflammatory response and they are involved in epithelial and endothelial repair after injury. Here, our study demonstrates MSCs therapeutic potential in a rat model of ALI/ARDS. Methods Bone marrow derived MSCs were obtained from Sprague-Dawley (SD) rats and their differential potential was verified. ALl was induced in rats byoleic acid (OA), and MSCs were transplanted intravenously. The lung injury and the concentration of cytokines in plasma and lung tissue extracts were assessed at 8 hours, 24 hours and 48 hours after OA-injection. Results The histological appearance and water content in rat lung tissue were significantly improved at different time points in rats treated with MSCs. The concentration of tumor necrosis factor-a and intercellular adhesion molecular-1 in rats plasma and lung tissue extracts were significantly inhibited after intravenous transplantation of MSCs, whereas interleukin-10 was significantly higher after MSCs transplantation at 8 hours, 24 hours and 48 hours after OA-challenge. Conclusions Intravenous transplantation of MSCs could maintain the integrity of the pulmonary alveolar-capillary barrier and modulate the inflammatory response to attenuate the experimental ALI/ARDS. Transplantation of MSCs could be a novel cell-based therapeutic strategy for prevention and treatment of ALI/ARDS.展开更多
BACKGROUND Meningioma in the cerebellopontine angle(CPA)without dural attachment is extremely rare.We report a unique case of meningioma derived from the superior petrosal vein without dural attachment.CASE SUMMARY A ...BACKGROUND Meningioma in the cerebellopontine angle(CPA)without dural attachment is extremely rare.We report a unique case of meningioma derived from the superior petrosal vein without dural attachment.CASE SUMMARY A 44-year-old right-handed woman presented with a two-month history of headache and tinnitus.Brain magnetic resonance imaging showed a well-defined contrast-enhancing lesion in the right CPA without a dural tail sign.Tumor resection was performed using a right retro sigmoid approach.A dural attachment was not seen at the tentorium or posterior surface of the petrous pyramid.The tumor was firmly adherent to the superior petrosal vein.The origin site was cauterized and resected with the preservation of the superior petrosal vein.A diagnosis of meningothelial meningioma was made.The patient’s headache and tinnitus gradually disappeared,and a recurrence was not observed five years after the surgery.CONCLUSION The rare occurrence of meningioma without dural attachment makes it difficult to determine dural attachment before surgery.The absence of dural attachment makes it easy to completely resect such tumors.Vessels related to tumors should be removed carefully,considering the possible presence of tumor stem cells in the microvessels.展开更多
This study aims to investigate the clinical response of ATP tumor chemosensitivity assay(ATP-TCA)directed chemotherapy regimens delivered via hepatic artery infusion in 104 cases of primary liver carcinoma(PLC).Tumor ...This study aims to investigate the clinical response of ATP tumor chemosensitivity assay(ATP-TCA)directed chemotherapy regimens delivered via hepatic artery infusion in 104 cases of primary liver carcinoma(PLC).Tumor tissue was obtained via laparotomy and cultivated in vitro.This tissue was put through the assay to determine chemosensitivity.A single drug regimen of either 5-FU,MMC and ADM and a combination drug regimen were used.The treatment assigned was dependent on the result of the ATP-TCA.In the control group,30 cases of diagnosed PLC were given the conventional three-combination drug.The two groups were evaluated after three courses of chemotherapy.The results are as follows.The overall response rate of sensitivity test ranged from 36%to 44%in the single drug therapy groups and 81%in the combination drug group.The clinical overall response rate was 75%in the treatment group and 56%in control group.The treatment group had better results than the control group as survival period over six months was 80% and over one year 44%.In the control group,survival period over six months was 60% and 30% over one year.In short,ATP-TCA directed chemotherapy shows better results for terminal stages of PLC in that you can decrease the dose of drugs thereby reducing the side-effects with possible improvements in therapeutic effects.展开更多
基金Supported by Science and Technology Research Project of Chongqing Municipal Education Commission(KJ111307)
文摘Taking tumorous stem mustard infected by black spot disease as the research material, the ribosomal 5.8S rDNA and its flanking ITS region were cloned, sequenced and aligned in the study. The results showed that the base sequence of pathogens collected from five different sites was almost identical with that of Alternaria brassicae, and the similarity degree reached 99.68%, without base difference greater than 3 bp. It had obvious differences with the sequences of A. brassicicola and A. japonica, and the base difference was greater than 3 bp with a lot of fragment deletions. It was preliminarily determined that the pathogen cau- sing black spot disease on tumorous stem mustard was A. brassicae.
文摘Objective: To explore the role of miR-448 in regulating MAGEA6/AMPK signaling pathway in the biological study of hepatocellular carcinoma (HCC) tumor stem cells. Methods: Using the database, the hepatocellular carcinoma related expression chips were obtained and the regulatory mirnas of candidate genes were predicted, and the predicted results were analyzed. The effects of miR-448 and MAGEA6 on the pellet formation rate and clone formation rate of hepatocellular carcinoma stem cells were detected by immunofluorescence identification of stem cell markers and light microscope counting method. The effects of miR-448 and MAGEA6 on migration and invasion of hepatocellular carcinoma stem cells were detected by scratch and Transwell assay. Dual luciferase reporter assay to verify whether miR-448 targets MAGEA6. The expression and influence of miR-448 on MAGEA6 and AMPK pathway were detected by qRT-PCR and Western blot. Results: It was found that miR-448 may directly regulate the expression of MAGEA6. Overexpression of miR-448 inhibited the characteristics, proliferation, migration, and invasion of hepatocellular carcinoma stem cells in vitro, as well as the ability of xenograft tumor formation in vivo. However, inhibition of miR-448 showed opposite results. In addition, miR-448 directly targets MAGEA6 and regulates AMPK signaling. Silencing MAGEA6 and adding AMPK activator further verified that miR-448 activated AMPK signaling pathway by targeting MAGEA6, thus affecting characteristics, proliferation, migration and invasion of hepatoma stem cells. Conclusions: Our results reveal that miR-448 activates AMPK signaling pathway by targeting MAGEA6, thereby affecting characteristics, proliferation, migration and invasion of hepatoma stem cells. It is suggested that overexpression of miR-448 may be a new therapeutic strategy for hepatocellular carcinoma.
基金Supported by the Natural Science Foundation of Zhejiang Province of China,No. LY18C070002 and No.LY16H160056National Natural Science Foundation of China,No.81803069+1 种基金the 521 Talent Project of Zhejiang Sci-Tech UniversityScience Foundation of Zhejiang Sci Tech University,No. 18042291Y。
文摘Cancer cells possess metabolic properties that are different from those of benign cells.p21,encoded by CDKN1A gene,also named p21Cip1/WAF1,was first identified as a cyclin-dependent kinase regulator that suppresses cell cycle G1/S phase and retinoblastoma protein phosphorylation.CDKN1A(p21)acts as the downstream target gene of TP53(p53),and its expression is induced by wild-type p53 and it is not associated with mutant p53.p21 has been characterized as a vital regulator that involves multiple cell functions,including G1/S cell cycle progression,cell growth,DNA damage,and cell stemness.In 1994,p21 was found as a tumor suppressor in brain,lung and colon cancer by targeting p53 and was associated with tumorigenesis and metastasis.Notably,p21 plays a significant role in tumor development through p53-dependent and p53-independent pathways.In addition,expression of p21 is closely related to the resting state or terminal differentiation of cells.p21 is also associated with cancer stem cells and acts as a biomarker for such cells.In cancer therapy,given the importance of p21 in regulating the G1/S and G2 check points,it is not surprising that p21 is implicated in response to many cancer treatments and p21 promotes the effect of oncolytic virotherapy.
文摘Although it is believed that glioma is derived from brain tumor stem cells, the source and molecular signal pathways of these cells are still unclear. In this study, we used stable doxycycline-inducible transgenic mouse brain tumor models (c-myc/SV40Tag+/Tet-on+) to explore the malignant trans- formation potential of neural stem cells by observing the differences of neural stem cells and brain tumor stem cells in the tumor models. Results showed that chromosome instability occurred in brain tumor stem cells. The numbers of cytolysosomes and autophagosomes in brain tumor stem cells and induced neural stem cells were lower and the proliferative activity was obviously stronger than that in normal neural stem cells. Normal neural stem cells could differentiate into glial fibrillary acidic protein-positive and microtubule associated protein-2-positive cells, which were also negative for nestin. However, glial fibrillary acidic protein/nestin, microtubule associated protein-2/nestin, and glial fibrillary acidic protein/microtubule associated protein-2 double-positive cells were found in induced neural stem cells and brain tumor stem cells. Results indicate that induced neural stem cells are similar to brain tumor stem cells, and are possibly the source of brain tumor stem cells.
基金Shandong Medical and Health Development Plan(2018WS423).
文摘Extensive in vitro and in vivo research reveals multiple intracellular molecular targets of Antrodia camphorata,and these targets affect growth,apoptosis,angiogenesis,invasion and metastasis of cells.These targets include tumor suppressor,cell cycle regulator,transcription factor,angiogenesis and metastasis factor,apoptosis and survival regulator,etc.Additionally,more and more attention has been paid to the molecular mechanism of A.camphorata on the regulation of tumor stem cells.Meanwhile,there is evidence that the immunoregulation of A.camphorata is enhanced,which may lead cell cycle arrest or apoptosis.In this paper,molecular mechanism of tumor cells and tumor stem cells regulated at multiple targets by A.camphorata in vitro and in vivo in the past decade is summarized.
文摘Objective To investigate the effect of dendritic cells pulsed with brain tumor stem cells which are used to treat on intracranial glioma. Methods We obtained murine brain tumor stem cells by grow ing C6 cells in epidermal grow th factor/basic fibroblast grow th factor w ithout serum.Dendritic cells isolated from rat bone marrow w ere pulsed w ith BTSCs. Rat brain
基金supported by a grant from the National Natural Science Foundation of China(30801100)
文摘BACKGROUND:Emerging evidence suggests that pancreatic adenocarcinoma is hierarchically organized and sustained by pancreatic cancer stem cells.Furthermore,elimination of these cells is possible and therapeutically relevant.This study aimed to investigate the expression patterns of pancreatic cancer stem cell surface markers CD44,CD24 and ESA in pancreatic adenocarcinoma cell lines and explore the influence of their local microenvironment.METHODS:Flow cytometry was used to analyze the expression patterns of CD44,CD24 and ESA in five pancreatic adenocarcinoma cell lines (PANC-1,PC-2,MIA-Paca-2,AsPC-1 and BxPC-3).In addition,the capacity for sphereformation in serum-free medium of four cell lines (PANC-1,PC-2,MIA-Paca-2 and BxPC-3) was assessed.Then,the same assays were performed when tumor cell spheres were developed.The role of sonic hedgehog (SHH) in cell spheres from PANC-1 and MIA-Paca-2 were also assessed by RT-PCR.RESULTS:CD44 and CD24 were detected in PANC-1.Only CD44 expression was detected in PC-2,MIA-Paca-2 and AsPC-1.CD44,CD24 and ESA were all detected in BxPC-3.Tumor cell spheres developed in PANC-1 and MIA-Paca-2 in serumfree medium.This was accompanied by an increase in CD24 expression and a decrease in CD44 expression in PANC-1.Interestingly,the expression of CD44 and CD24 returned to initial levels once the medium was changed back from serumfree to serum-containing medium.No significant change in the expression of CD44 was detected in MIA-Paca-2.Furthermore,the relative quantification of SHH mRNA in PANC-1 cell spheres was significantly higher than that in cells cultured in the serum-containing medium.CONCLUSION:The expression patterns of the pancreatic cancer stem cell surface markers CD44,CD24 and ESA were diverse in different pancreatic adenocarcinoma cell lines and changed with their local microenvironment.
文摘Background:We have shown that downregulation of materally expressed gene 3(MEG3)promoted autophagy and epithelial-mesenchymal transition to promote neuroblastoma(NB)progression.In present study,we aim to further discover the role of MEG3 in NB.Methods:Expression levels of MEG3 in stabled transfected cells were detected by qPCR.Flow cytometry was used to examine the effects of MEG3 overexpression on apoptosis and cell cycle.Moreover,tumor stemness was detected by tumor sphere formation assay.Results:QPCR showed that MEG3 was successfully overexpressed in SK-N-BE(2)C and SK-N-AS cells.CCK8 indicated MEG3 reduced cell proliferation in two NB cells.Flow cytometry revealed that MEG3 promoted apoptosis and caused G0/G1 arrest.Using chromatin isolation by RNA purification(ChIRP)and tumor sphere formation assays,we revealed that upregulation of MEG3 decreased tumor stemness by decreasing Nestin transcription.Conclusions:Overall,present study confirmed an anti-cancer role of MEG3 in NB,demonstrated that MEG3 could be a potential therapeutic target of NB.
基金supported by the National Natural Science Foundation of China(82203163)the Natural Science Foundation of Hunan Province(2022JJ40660)+1 种基金the Natural Science Foundation of Changsha(kq2202123)the Young Elite Scientists Sponsorship Program by CAST(2022QNRC001)。
文摘Adhesion molecules mediate cell-to-cell and cell-to-extracellular matrix interactions and transmit mechanical and chemical signals among them.Various mechanisms deregulate adhesion molecules in cancer,enabling tumor cells to proliferate without restraint,invade through tissue boundaries,escape from immune surveillance,and survive in the tumor microenvironment.Recent studies have revealed that adhesion molecules also drive angiogenesis,reshape metabolism,and are involved in stem cell self-renewal.In this review,we summarize the functions and mechanisms of adhesion molecules in cancer and the tumor microenvironment,as well as the therapeutic strategies targeting adhesion molecules.These studies have implications for furthering our understanding of adhesion molecules in cancer and providing a paradigm for exploring novel therapeutic approaches.
基金This study was supported by a grant from National Natural Science Foundation of China (No. 81070055).
文摘Background Acute lung injury (ALl) and end-stage acute respiratory distress syndrome (ARDS) were among the most common causes of death in intensive care units. The activation of an inflammatory response and the damage of pulmonary epithelium and endotheliumwerethe hallmark of ALI/ARDS. Recent studies had demonstrated the importance of mesenchymal stem cells (MSCs) in maintaining the normal pulmonary endothelial and epithelial function as well as participating in modulating the inflammatory response and they are involved in epithelial and endothelial repair after injury. Here, our study demonstrates MSCs therapeutic potential in a rat model of ALI/ARDS. Methods Bone marrow derived MSCs were obtained from Sprague-Dawley (SD) rats and their differential potential was verified. ALl was induced in rats byoleic acid (OA), and MSCs were transplanted intravenously. The lung injury and the concentration of cytokines in plasma and lung tissue extracts were assessed at 8 hours, 24 hours and 48 hours after OA-injection. Results The histological appearance and water content in rat lung tissue were significantly improved at different time points in rats treated with MSCs. The concentration of tumor necrosis factor-a and intercellular adhesion molecular-1 in rats plasma and lung tissue extracts were significantly inhibited after intravenous transplantation of MSCs, whereas interleukin-10 was significantly higher after MSCs transplantation at 8 hours, 24 hours and 48 hours after OA-challenge. Conclusions Intravenous transplantation of MSCs could maintain the integrity of the pulmonary alveolar-capillary barrier and modulate the inflammatory response to attenuate the experimental ALI/ARDS. Transplantation of MSCs could be a novel cell-based therapeutic strategy for prevention and treatment of ALI/ARDS.
文摘BACKGROUND Meningioma in the cerebellopontine angle(CPA)without dural attachment is extremely rare.We report a unique case of meningioma derived from the superior petrosal vein without dural attachment.CASE SUMMARY A 44-year-old right-handed woman presented with a two-month history of headache and tinnitus.Brain magnetic resonance imaging showed a well-defined contrast-enhancing lesion in the right CPA without a dural tail sign.Tumor resection was performed using a right retro sigmoid approach.A dural attachment was not seen at the tentorium or posterior surface of the petrous pyramid.The tumor was firmly adherent to the superior petrosal vein.The origin site was cauterized and resected with the preservation of the superior petrosal vein.A diagnosis of meningothelial meningioma was made.The patient’s headache and tinnitus gradually disappeared,and a recurrence was not observed five years after the surgery.CONCLUSION The rare occurrence of meningioma without dural attachment makes it difficult to determine dural attachment before surgery.The absence of dural attachment makes it easy to completely resect such tumors.Vessels related to tumors should be removed carefully,considering the possible presence of tumor stem cells in the microvessels.
文摘This study aims to investigate the clinical response of ATP tumor chemosensitivity assay(ATP-TCA)directed chemotherapy regimens delivered via hepatic artery infusion in 104 cases of primary liver carcinoma(PLC).Tumor tissue was obtained via laparotomy and cultivated in vitro.This tissue was put through the assay to determine chemosensitivity.A single drug regimen of either 5-FU,MMC and ADM and a combination drug regimen were used.The treatment assigned was dependent on the result of the ATP-TCA.In the control group,30 cases of diagnosed PLC were given the conventional three-combination drug.The two groups were evaluated after three courses of chemotherapy.The results are as follows.The overall response rate of sensitivity test ranged from 36%to 44%in the single drug therapy groups and 81%in the combination drug group.The clinical overall response rate was 75%in the treatment group and 56%in control group.The treatment group had better results than the control group as survival period over six months was 80% and over one year 44%.In the control group,survival period over six months was 60% and 30% over one year.In short,ATP-TCA directed chemotherapy shows better results for terminal stages of PLC in that you can decrease the dose of drugs thereby reducing the side-effects with possible improvements in therapeutic effects.