Taking tumorous stem mustard infected by black spot disease as the research material, the ribosomal 5.8S rDNA and its flanking ITS region were cloned, sequenced and aligned in the study. The results showed that the ba...Taking tumorous stem mustard infected by black spot disease as the research material, the ribosomal 5.8S rDNA and its flanking ITS region were cloned, sequenced and aligned in the study. The results showed that the base sequence of pathogens collected from five different sites was almost identical with that of Alternaria brassicae, and the similarity degree reached 99.68%, without base difference greater than 3 bp. It had obvious differences with the sequences of A. brassicicola and A. japonica, and the base difference was greater than 3 bp with a lot of fragment deletions. It was preliminarily determined that the pathogen cau- sing black spot disease on tumorous stem mustard was A. brassicae.展开更多
With the acknowledgement of species, symptoms and control measures for diseases, pests and weeds in tumorous stem mustard, the expert prevention system has been studied and developed based on internct, and the system ...With the acknowledgement of species, symptoms and control measures for diseases, pests and weeds in tumorous stem mustard, the expert prevention system has been studied and developed based on internct, and the system mainly includes knowledge database, inference engine, browser web and so on. The knowledge database has been established by Micrsoft Access 2003 software; the procedure of inference engine has been compiled by JavaScript; the pages of browser web have been made by Dreamweaver MX software. The expert system is fuR-featured and user-friendly, which can provide control knowledge against the diseases, pests and weeds of tumorous stem mustard for the majority of farmers, scientific technological person and grass-roots level managers quickly and conveniently,展开更多
基金Supported by Science and Technology Research Project of Chongqing Municipal Education Commission(KJ111307)
文摘Taking tumorous stem mustard infected by black spot disease as the research material, the ribosomal 5.8S rDNA and its flanking ITS region were cloned, sequenced and aligned in the study. The results showed that the base sequence of pathogens collected from five different sites was almost identical with that of Alternaria brassicae, and the similarity degree reached 99.68%, without base difference greater than 3 bp. It had obvious differences with the sequences of A. brassicicola and A. japonica, and the base difference was greater than 3 bp with a lot of fragment deletions. It was preliminarily determined that the pathogen cau- sing black spot disease on tumorous stem mustard was A. brassicae.
基金Supported by Yangtze Normal University Research Projects of Young Teachers(09JKY071)~~
文摘With the acknowledgement of species, symptoms and control measures for diseases, pests and weeds in tumorous stem mustard, the expert prevention system has been studied and developed based on internct, and the system mainly includes knowledge database, inference engine, browser web and so on. The knowledge database has been established by Micrsoft Access 2003 software; the procedure of inference engine has been compiled by JavaScript; the pages of browser web have been made by Dreamweaver MX software. The expert system is fuR-featured and user-friendly, which can provide control knowledge against the diseases, pests and weeds of tumorous stem mustard for the majority of farmers, scientific technological person and grass-roots level managers quickly and conveniently,
