目的:研究肿瘤相关钙信号传导蛋白2(tumor-associated calcium signal transducer-2,Trop-2)表达下调对食管鳞癌EC9706细胞增殖和细胞迁移的影响,并探讨其可能的分子机制.方法:将Trop-2siRNA和对照siRNA转染食管鳞癌EC9706细胞,利用实...目的:研究肿瘤相关钙信号传导蛋白2(tumor-associated calcium signal transducer-2,Trop-2)表达下调对食管鳞癌EC9706细胞增殖和细胞迁移的影响,并探讨其可能的分子机制.方法:将Trop-2siRNA和对照siRNA转染食管鳞癌EC9706细胞,利用实时荧光定量PCR和Western blotting检测转染Trop-2siRNA后食管鳞癌EC9706细胞中Trop-2mRNA和蛋白的表达.利用CCK-8试剂分析转染Trop-2siRNA后EC9706细胞的增殖变化,接着采用Boyden小室研究下调Trop-2对EC9706细胞迁移的影响,最后利用Western blotting技术分析与细胞迁移相关基因MMP-7表达的变化.结果:转染Trop-248h,Trop-2siRNA组中Trop-2mRNA和蛋白的表达显著低于未处理组和对照siRNA组(P<0.05).Trop-2表达下调能明显抑制食管鳞癌EC9706细胞的增殖(P<0.05).此外,Boyden小室结果表明,与未处理组(119.27±4.63)及对照siRNA组(112.81±5.01)相比,Trop-2siRNA组中EC9706细胞的穿膜数(65.29±4.33)明显降低.Western blotting结果表明,Trop-2siRNA能明显降低食管鳞癌EC9706细胞中MMP-7的表达.结论:Trop-2表达下调能抑制食管鳞癌细胞的增殖和迁移,其介导的迁移能力的降低可能与MMP-7表达的下调密切相关,操纵Trop-2有望成为食管鳞癌的分子治疗靶点.展开更多
目的探讨肿瘤相关钙信号转导蛋白2(TACSTD2)在甲状腺乳头状癌(PTC)组织中的表达及其临床病理意义。方法收集2014年2月至2018年2月就诊于福建省立医院基本外科的130例PTC患者的临床病理资料及术后标本。采用免疫组织化学法,对甲状腺中癌...目的探讨肿瘤相关钙信号转导蛋白2(TACSTD2)在甲状腺乳头状癌(PTC)组织中的表达及其临床病理意义。方法收集2014年2月至2018年2月就诊于福建省立医院基本外科的130例PTC患者的临床病理资料及术后标本。采用免疫组织化学法,对甲状腺中癌组织及相应癌旁正常甲状腺组织进行检测,分析两种组织中T A C S T D 2的表达情况,并将其与患者的临床病理特征进行统计学分析。结果TACSTD2在PTC的癌组织中的表达阳性率显著高于癌旁正常甲状腺组织(P<0.001)。单因素卡方检验表明,TACSTD2表达与肿瘤大小(P=0.002)、被膜侵犯(P<0.001)、淋巴结转移(P=0.006)显著相关。多因素logistic回归分析表明,TACSTD2表达与被膜侵犯(P=0.007)和淋巴结转移(P=0.001)呈独立相关性。结论PTC患者的癌组织中存在TACSTD2过表达的情况,其过表达与被膜侵犯、淋巴结转移有关,提示TACSTD2过表达可能参与PTC的局部侵袭及淋巴结转移过程。展开更多
Colorectal cancer (CRC) is an important health issue in Taiwan. There were over ten thousand newly diagnosed CRC patients each year. The outcome of late stage CRC still remains to be improved, and tumor markers are ex...Colorectal cancer (CRC) is an important health issue in Taiwan. There were over ten thousand newly diagnosed CRC patients each year. The outcome of late stage CRC still remains to be improved, and tumor markers are expected to improve CRC detection and management. From a colorectal cancer cell secretome database, we chose four proteins as candidates for clinical verification, including tumor-associated calcium signal transducer 2 (TROP2, TACSTD2), transmembrane 9 superfamily member 2 (TM9SF2), and tetraspanin-6 (TSPAN6), and tumor necrosis factor receptor superfamily member 16 (NGFR). Different groups of 30 CRC patients’ tissue samples collected from Chang Gung Memorial Hospital were analyzed by immunohistochemistry (IHC) for the four proteins, and the results were scored by pathologist. For all the four candidate proteins, marked differences of IHC score existed between tumor and adjacent non-tumor counterpart. However, there were only trends between higher protein expression levels and worse outcome. Three proteins (TROP2, TM9SF2 and NGFR) had trends between higher tissue expression and tumor stage or lymph node metastasis. Our study revealed that tissue expression of four proteins (TROP2, TM9SF2, TSPAN6, and NGFR) was markedly different between tumor and adjacent non-tumor counterparts. Overexpression of all these four proteins showed some trends with poorer survival.展开更多
文摘目的探讨肿瘤相关钙信号转导蛋白2(TACSTD2)在甲状腺乳头状癌(PTC)组织中的表达及其临床病理意义。方法收集2014年2月至2018年2月就诊于福建省立医院基本外科的130例PTC患者的临床病理资料及术后标本。采用免疫组织化学法,对甲状腺中癌组织及相应癌旁正常甲状腺组织进行检测,分析两种组织中T A C S T D 2的表达情况,并将其与患者的临床病理特征进行统计学分析。结果TACSTD2在PTC的癌组织中的表达阳性率显著高于癌旁正常甲状腺组织(P<0.001)。单因素卡方检验表明,TACSTD2表达与肿瘤大小(P=0.002)、被膜侵犯(P<0.001)、淋巴结转移(P=0.006)显著相关。多因素logistic回归分析表明,TACSTD2表达与被膜侵犯(P=0.007)和淋巴结转移(P=0.001)呈独立相关性。结论PTC患者的癌组织中存在TACSTD2过表达的情况,其过表达与被膜侵犯、淋巴结转移有关,提示TACSTD2过表达可能参与PTC的局部侵袭及淋巴结转移过程。
文摘Colorectal cancer (CRC) is an important health issue in Taiwan. There were over ten thousand newly diagnosed CRC patients each year. The outcome of late stage CRC still remains to be improved, and tumor markers are expected to improve CRC detection and management. From a colorectal cancer cell secretome database, we chose four proteins as candidates for clinical verification, including tumor-associated calcium signal transducer 2 (TROP2, TACSTD2), transmembrane 9 superfamily member 2 (TM9SF2), and tetraspanin-6 (TSPAN6), and tumor necrosis factor receptor superfamily member 16 (NGFR). Different groups of 30 CRC patients’ tissue samples collected from Chang Gung Memorial Hospital were analyzed by immunohistochemistry (IHC) for the four proteins, and the results were scored by pathologist. For all the four candidate proteins, marked differences of IHC score existed between tumor and adjacent non-tumor counterpart. However, there were only trends between higher protein expression levels and worse outcome. Three proteins (TROP2, TM9SF2 and NGFR) had trends between higher tissue expression and tumor stage or lymph node metastasis. Our study revealed that tissue expression of four proteins (TROP2, TM9SF2, TSPAN6, and NGFR) was markedly different between tumor and adjacent non-tumor counterparts. Overexpression of all these four proteins showed some trends with poorer survival.
