Separation and Identification of Differentially Expressed Proteins in Pistillate Flowers between Different Mulberry Cultivars

[Objective] This study aimed to explore the proteins related to pistillate flower development in different mulberry cultivars. [Method] The total proteins of the pistillate flowers of two mulberry cultivars Dal0 （Morus atropurpurea Roxb.） and SG01 （Morus muIticaulis Perr.） were extracted, separated and detected through two- dimensional electrophoresis （2-DE） and mass spectrometry. [Result] There was sig- nificant difference in the expression of proteins from the pistillate flowers of different mulberry cultivars. From the 2-DE images of Dal0 and SG01, 445_＋17 and 425_＋12 protein spots were respectively detected. The expression levels of 75 protein spots differed significantly. Thirteen spots those were expressed at high levels and well separated were analyzed by mass spectrometry, and nine of them were identified successfully. The nine proteins are involved in the glycometabolism, protein and amino acid metabolism and defense responses during the development of mulberry pistillate flower after they were pollinated. [Conclusion] The findings will provide reference for further study on the molecular mechanism of mulberry pistillate flower de- velopment.

蛋白质组研究的进展

蛋白质组研究的目的在于利用双向凝胶电泳、质谱技术等手段从整体上研究蛋白质的水平和修饰状态 ,以便阐明生命现象的本质和活动规律 .通过对细菌、酵母等模式生物蛋白质组研究 ,人类蛋白质组研究取得了一定进展 .蛋白质组研究速度的提高有赖于新技术手段的应用和生物信息学的发展 .揭示蛋白质之间的相互作用关系并建立其网络图是蛋白质组研究的热点 .蛋白质组研究成果的应用将带来工业、农业。

人胆囊组织蛋白质表达谱的建立

目的:建立胆固醇结石病人与正常人胆囊组织的蛋白质表达谱,用于比较胆囊组织的蛋白质组学研究,为阐明胆石成因提供实验依据。方法:提取胆固醇结石病人及正常人的胆囊壁组织总蛋白,构建相应的双向电泳表达图谱,应用ImageMaster图像分析软件对双向电泳图谱进行图像分析。结果:两组样本均获得分辨率高、重复性好的双向电泳图谱,胆固醇结石组与正常组病人组内蛋白质平均匹配点数分别为632±61和606±64,各匹配率分别为73.46%和71.49%。组间蛋白质平均匹配点为474,匹配率为70.64%。胆固醇结石病人的28个蛋白点表达上调,7个点表达下调,与正常人间蛋白表达差异明显(P<0.05)。结论:初步建立了胆固醇结石病人与正常人胆囊组织的差异蛋白质表达谱,分析两者间差异蛋白点,为胆石成因研究提供实验基础。