Effect of Mouth Opening Training Stick Combined with Oral Massage on Mouth Opening Difficulty in Head and Neck Cancer Patients after Treatment

Objective: To study the application effect of mouth-opening training sticks combined with oral massage on patients with mouth-opening difficulty after treatment for head and neck cancer. Methods: Using convenient sampling, 60 patients with mouth-opening difficulty after treatment for head and neck cancer admitted to the Oncology Department from February 2022 to October 2023 were selected for a 2-week exercise and nursing program. The patients were divided into a control group (February 1, 2022 to November 30, 2022) and an observation group (December 1, 2022 to October 31, 2023), with 30 patients in each group. The control group underwent routine mouth-opening functional exercises combined with cork for oral support training, while the observation group underwent routine mouth-opening functional exercises combined with oral massage and mouth-opening training sticks for oral support training. The mouth-opening degree, mouth-opening difficulty level, comfort level, compliance, and quality of life were observed in both groups. Results: Before the intervention, there were no statistically significant differences in mouth-opening degree and mouth-opening difficulty level between the two groups (P > 0.05). After the intervention, the mouth-opening degree, mouth-opening difficulty level, oral comfort level, compliance, and QLICP-HN scores in the observation group were all better than those in the control group, with statistically significant differences (P < 0.05). Conclusion: The combination of mouth-opening training sticks and oral massage with mouth-opening functional exercises can effectively improve the degree of mouth-opening difficulty, enhance oral comfort, increase compliance with mouth-opening exercises, and improve quality of life.

Geometry and 3D seismic characterisation of post-rift normal faults in the Pearl River Mouth Basin,northern South China Sea

Based on high-resolution 3D seismic data acquired in the Pearl(Zhujiang)River Mouth Basin of the northern South China Sea,this study investigated the geometry,spatial extension,and throw distribution of the post-rift normal fault through detailed seismic interpretation and fault modeling.A total of 289 post-rift normal faults were identified in the study area and can be classified into four types:(1)isolated normal faults above the carbonate platform;(2)isolated normal faults cutting through the carbonate platform;(3)conjugate normal faults,and(4)connecting normal faults.Throw distribution analysis on the fault planes show that the vertical throw profiles of most normal fault exhibit flat-topped profiles.Isolated normal faults above the carbonate platform exhibit roughly concentric ellipses with maximum throw zones in the central section whereas the normal faults cutting through the carbonate platform miss the lowermost section due to the chaotic seismic reflections in the interior of the carbonate platform.The vertical throws of conjugate normal faults anomalously decrease toward their intersection region on the fault plane whereas the connecting normal faults present two maximum throw zones in the central section of the fault plane.According to the symmetric elliptical distribution model of fault throw,an estimation was made indicating that normal faults cutting through the carbonate platform extended downward between-1308 s and-1780 s(two-way travel time)in depth and may not penetrate the entire Liuhua carbonate platform.Moreover,it is observed that the distribution of karst caves on the top of the carbonate platform disaccord with those of hydrocarbon reservoirs and the post-rift normal faults cutting through the carbonate platform in the study area.We propose that these karst caves formed most probably by corrosive fluids derived from magmatic activities during the Dongsha event,rather than pore waters or hydrocarbons.

Characteristics of transfer zones under the influence of preexisting faults and regional stress transformation:Wenchang A subsag,Zhujiang River Mouth Basin,northern South China Sea

A transfer zone in rift basins preserves important information on regional tectonic evolution and plays significant roles in hydrocarbon accumulation.Based on the systematic analysis of 3D seismic data and hydrocarbon accumulation conditions,the geometry,kinematics,and reservoir control of a large synthetic overlapping transfer zone in the south of the Wenchang A subsag in the Zhujiang(Pearl)River Mouth basin were investigated.Results indicate that the development and evolution of the transfer zone was controlled by the interaction between pre-existing faults and regional stress transformation.The intense rifting of the main faults of the transfer zone controlled the development of source rocks and faultcontrolled slope break paleogeomorphology.The strike-slip overprint since the Oligocene is conducive to the formation of a large-scale fault-anticline trap,and the secondary faults in the transfer zone contribute to the hydrocarbon transportation.The conjugate intersection area of the NE-and NW-trending faults offers more opportunity for hydrocarbon migration and accumulation.

Discovery and inspiration of large-and medium-sized glutenite-rich oil and gas fields in the eastern South China Sea:An example from Paleogene Enping Formation in Huizhou 26 subsag,Pearl River Mouth Basin

Based on the practice of oil and gas exploration in the Huizhou Sag of the Pearl River Mouth Basin,the geochemical indexes of source rocks were measured,the reservoir development morphology was restored,the rocks and minerals were characterized microscopically,the measured trap sealing indexes were compared,the biomarker compounds of crude oil were extracted,the genesis of condensate gas was identified,and the reservoir-forming conditions were examined.On this basis,the Paleogene Enping Formation in the Huizhou 26 subsag was systematically analyzed for the potential of oil and gas resources,the development characteristics of large-scale high-quality conglomerate reservoirs,the trapping effectiveness of faults,the hydrocarbon migration and accumulation model,and the formation conditions and exploration targets of large-and medium-sized glutenite-rich oil and gas fields.The research results were obtained in four aspects.First,the Paleogene Wenchang Formation in the Huizhou 26 subsag develops extensive and thick high-quality source rocks of semi-deep to deep lacustrine subfacies,which have typical hydrocarbon expulsion characteristics of"great oil generation in the early stage and huge gas expulsion in the late stage",providing a sufficient material basis for hydrocarbon accumulation in the Enping Formation.Second,under the joint control of the steep slope zone and transition zone of the fault within the sag,the large-scale near-source glutenite reservoirs are highly heterogeneous,with the development scale dominated hierarchically by three factors(favorable facies zone,particle component,and microfracture).The(subaqueous)distributary channels near the fault system,with equal grains,a low mud content(<5%),and a high content of feldspar composition,are conducive to the development of sweet spot reservoirs.Third,the strike-slip pressurization trap covered by stable lake flooding mudstone is a necessary condition for oil and gas preservation,and the NE and nearly EW faults obliquely to the principal stress have the best control on traps.Fourth,the spatiotemporal configuration of high-quality source rocks,fault transport/sealing,and glutenite reservoirs controls the degree of hydrocarbon enrichment.From top to bottom,three hydrocarbon accumulation units,i.e.low-fill zone,transition zone,and high-fill zone,are recognized.The main area of the channel in the nearly pressurized source-connecting fault zone is favorable for large-scale hydrocarbon enrichment.The research results suggest a new direction for the exploration of large-scale glutenite-rich reservoirs in the Enping Formation of the Pearl River Mouth Basin,and present a major breakthrough in oil and gas exploration.

Expression and clinical significance of pattern recognition receptor-associated genes in hand, foot and mouth disease

Objective:To explore which pattern recognition receptors(PRRs)play a key role in the development of hand,foot,and mouth disease(HFMD)by analyzing PRR-associated genes.Methods:We conducted a comparative analysis of PRR-associated gene expression in human peripheral blood mononuclear cells(PBMCs)infected with enterovirus 71(EV-A71)which were derived from patients with HFMD of different severities and at different stages.A total of 30 PRR-associated genes were identified as significantly upregulated both over time and across different EV-A71 isolates.Subsequently,ELISA was employed to quantify the expression of the six most prominent genes among these 30 identified genes,specifically,BST2,IRF7,IFI16,TRIM21,MX1,and DDX58.Results:Compared with those at the recovery stage,the expression levels of BST2(P=0.027),IFI16(P=0.016),MX1(P=0.046)and DDX58(P=0.008)in the acute stage of infection were significantly upregulated,while no significant difference in the expression levels of IRF7(P=0.495)and TRIM21(P=0.071)was found between different stages of the disease.The expression levels of BST2,IRF7,IFI16 and MX1 were significantly higher in children infected with single pathogen than those infected with mixed pathogens,and BST2,IRF7,IFI16 and MX1 expression levels were significantly lower in coxsackie B virus(COXB)positive patients than the negative patients.Expression levels of one or more of BST2,IRF7,IFI16,TRIM21,MX1 and DDX58 genes were correlated with PCT levels,various white blood cell counts,and serum antibody levels that reflect disease course of HFMD.Aspartate aminotransferase was correlated with BST2,MX1 and DDX58 expression levels.Conclusions:PRR-associated genes likely initiate the immune response in patients at the acute stage of HFMD.

Transmission Dynamics and Optimal Control Strategies of a Hand-Foot-Mouth Disease Model with Treatment and Vaccination Interventions

In this article, the transmission dynamics of a Hand-Foot-Mouth disease model with treatment and vaccination interventions are studied. We calculated the basic reproduction number and proved the global stability of disease-free equilibrium when R0 R0 > 1. Meanwhile, we obtained the optimal control strategies minimizing the cost of intervention and minimizing the infected person. We also give some numerical simulations to verify our theoretical results.

利用车载GNSS轨迹大数据的U-Turn道路结构信息获取方法

随着智慧交通和精细化导航技术的迅速发展,人们对道路地图要素的覆盖度、精准度、丰富度与新鲜度需求越来越高,而U-Turn作为城市路网连通关系的重要因素,成为道路地图更新的重要内容之一。现有专业道路测绘模式对U-Turn数据存在采集成本高、更新周期长、数据处理繁等问题,导致U-Turn数据无法满足智慧交通导航需求。本文采用车载GNSS轨迹大数据,提出了一种U-Turn道路结构信息获取方法。首先通过轨迹跟踪提取车辆掉头点对与行为;然后利用DBSCAN聚类算法提取U-Turn掉头类簇;再依据U-Turn流量占比等特征构建支持向量机二分模型,自适应剔除违规掉头类簇,并区分出U-Turn结构有无通行时间限制;最后根据掉头类簇在道路结构中的分布特征,识别U-Turn位置与空间结构。试验以武汉市滴滴网约车GNSS轨迹,对江汉区183个路段采用本文方法进行探测,U-Turn结构信息识别召回率为88.3%,精确率为87.6%,位置识别的横向和纵向精度分别为3.40 m和5.90 m,试验结果表明本文方法可以有效地从车载GNSS轨迹数据中获取U-Turn的位置与结构类型,为U-Turn数据获取提供了周期短、成本低的有效解决方案。

Computational Investigation of Hand Foot Mouth Disease Dynamics with Fuzziness

The first major outbreak of the severely complicated hand,foot and mouth disease(HFMD),primarily caused by enterovirus 71,was reported in Taiwan in 1998.HFMD surveillance is needed to assess the spread of HFMD.The parameters we use in mathematical models are usually classical mathematical parameters,called crisp parameters,which are taken for granted.But any biological or physical phenomenon is best explained by uncertainty.To represent a realistic situation in any mathematical model,fuzzy parameters can be very useful.Many articles have been published on how to control and prevent HFMD from the perspective of public health and statistical modeling.However,few works use fuzzy theory in building models to simulateHFMDdynamics.In this context,we examined anHFMD model with fuzzy parameters.A Non Standard Finite Difference(NSFD)scheme is developed to solve the model.The developed technique retains essential properties such as positivity and dynamic consistency.Numerical simulations are presented to support the analytical results.The convergence and consistency of the proposed method are also discussed.The proposed method converges unconditionally while the many classical methods in the literature do not possess this property.In this regard,our proposed method can be considered as a reliable tool for studying the dynamics of HFMD.

Construction of Plant Expression Vector for Hand,Foot and Mouth Virus EV71-VP1 Gene and Its Expression in Tomato

EV71-type virus is one of the main pathogens causing the occurrence of hand,foot and mouth disease(HFMD),and VP1 protein,a factor that directly determines the antigenicity of the virus,has been isolated.The tomato was selected as a bioreactor for the production of an edible EV71 vaccine designed for the VP1 capsid protein.Using molecular biology techniques,the fusion gene EV71-VP1 was cut from vector PGEX-4T-2,a vector containing the p2300-EV71 gene with CaMV35S promoter and TL regulatory elements was constructed,and the hypocotyl and cotyledons of tomato were transformed using Agrobacterium(EHA105)-mediated method,screened,elongated and rooted,and finally 20 resistant tomato plants were obtained.Five transgenic positive seedlings were obtained by digestion and PCR assay,among which three plants were detected by RT-PCR to be capable of transcriptional translation at the RNA level.The experimental results aimed to explore new material support for the preparation of transgenic plant oral vaccines against EV71 infection and provide a theoretical basis for accelerating the development of transgenic plant vaccines in the future.

Tectonic-thermal history and hydrocarbon potential of the Pearl River Mouth Basin,northern South China Sea:Insights from borehole apatite fission-track thermochronology

The Pearl River Mouth Basin(PRMB)is one of the most petroliferous basins on the northern margin of the South China Sea.Knowledge of the thermal history of the PRMB is significant for understanding its tectonic evolution and for unraveling its poorly studied source-rock maturation history.Our investigations in this study are based on apatite fission-track(AFT)thermochronology analysis of 12 cutting samples from 4 boreholes.Both AFT ages and length data suggested that the PRMB has experienced quite complicated thermal evolution.Thermal history modeling results unraveled four successive events of heating separated by three stages of cooling since the early Middle Eocene.The cooling events occurred approximately in the Late Eocene,early Oligocene,and the Late Miocene,possibly attributed to the Zhuqiong II Event,Nanhai Event,and Dongsha Event,respectively.The erosion amount during the first cooling stage is roughly estimated to be about 455-712 m,with an erosion rate of 0.08-0.12 mm/a.The second erosion-driven cooling is stronger than the first one,with an erosion amount of about 747-814 m and an erosion rate between about 0.13-0.21 mm/a.The erosion amount calculated related to the third cooling event varies from 800 m to 3419 m,which is speculative due to the possible influence of the magmatic activity.

Analysis of the Effect of Mindfulness Meditation on Patients with Burning Mouth Syndrome

Objective:To explore the effect of mindfulness meditation on patients with burning mouth syndrome.Methods:60 patients with burning mouth syndrome in our hospital who were treated from January 2021 to December 2022 were selected for this study.The patients were divided into two groups of thirty cases each using the randomized numerical table method.The observation underwent psychological intervention and mindfulness meditation training,while the control group only received symptomatic care.The condition of the patients of both groups was observed and compared.Results:Upon receiving treatment,the patients in the observation group had lower Hamilton Anxiety(HAM-A)scores,and Hamilton Depression(HAMD)scores compared to the control group(P<0.05).The visual analog scale(VAS)scores of the observation group were also lower than those of the control group(P<0.05).Moreover,the efficacy of the nursing intervention in the observation group was higher than that of the control group(P<0.05).Conclusion:Psychological intervention and mindfulness meditation training can effectively improve the clinical symptoms of patients with burning mouth syndrome.Therefore,this treatment method should be popularized.

珠江口盆地白云凹陷东部古近系文昌组沉积演化特征

充分利用新三维地震资料,结合测井以及录井资料,对珠江口盆地白云凹陷古近系文昌组层序地层、沉积特征及沉积演化进行研究,重新建立文昌组三级层序格架内的沉积体系演化过程。结果表明:白云凹陷东部文昌组自下而上共划分为5套三级层序,分别对应于初始裂陷期(WCSQ1)、拆离裂陷早期(WCSQ2,WCSQ3)、拆离裂陷晚期(WCSQ4,WCSQ5)3个构造演化阶段;初始裂陷期为多个孤立的箕状半地堑,以发育缓坡轴向物源辫状河三角洲、陡坡近源扇三角洲和滨浅湖沉积为特征;拆离裂陷早期断裂活动强烈,湖盆迅速扩张,半深湖—深湖局限发育,沉积中心北移,北部番禺低隆起发育大型辫状河三角洲沉积;拆离裂陷晚期断裂活动减弱,湖盆逐渐萎缩,沉积中心稳定在白云凹陷中部,主要发育小型辫状河三角洲沉积,滨浅湖发育;大型辫状河三角洲及扇三角洲砂体是白云凹陷东部的有利储集体。

基于双特异纳米抗体FMDV血凝检测方法的建立

本研究利用基因工程方法将抗口蹄疫病毒(FMDV)纳米抗体Nb205和抗鸡醛化红细胞(cRBC)纳米抗体NbRBC48的基因片段连接,构建双特异纳米抗体Nb205-48,其相对分子质量为3.5×10^(4),能同时与FMDV和cRBC反应。利用Nb205-48成功建立了可用于FMDV抗原检测的血凝方法,该方法检测灵敏度为1μg/ml,与其他病毒无交叉反应,且批内和批间重复性好。分别采用血凝法和夹心ELISA方法对3批次FMDV抗原进行了检测,二者检测结果基本吻合。本研究建立了基于双特异纳米抗体Nb205-48的血凝检测方法,该方法具有较好的灵敏度、特异性和重复性,且简便、快捷、成本低,其检测结果与传统的FMDV定量检测方法的检测结果相关性好,为检测口蹄疫疫苗生产过程中FMDV抗原的含量提供了新方法。

2020-2022年丽水市儿童手足口病流行特征及病原谱分析

目的分析2020—2022年丽水市儿童手足口病(HFMD)流行病学特征和病原学监测结果,为HFMD防控提供科学依据。方法通过传染病报告信息管理系统收集丽水市HFMD发病资料,对流行特征进行分析,同时采用荧光定量PCR技术对收集的1558例标本开展病原学检测,探究2020—2022年HFMD肠道病毒病原谱的动态变化。结果丽水市儿童HFMD发病年龄主要集中在0~5岁,2020年发病主要集中在9月至12月,2021—2022年主要集中在4月至7月份。2020—2022年间丽水市累计报告HFMD病例为13611例,在1558例病原学标本中检出肠道病毒核酸阳性1032份,总阳性率为66.24%。其中肠道病毒A组71型(EV71)5份,总阳性率为0.32%;柯萨奇病毒A组16型(CoxA16)322份,总阳性率为20.67%;柯萨奇病毒A组6型(CoxA6)型494份,总阳性率为31.71%;柯萨奇病毒A组10型(CoxA10型)63份,总阳性率为4.04%;其他型别148份,总阳性率9.5%。2020—2022年间,EV71仅在2021年检出5例;CoxA16型阳性率分别为2.44%、21.54%和36.42%,2022年1月至8月和11月至12月为检出高峰;CoxA6型阳性率分别为49.78%、33.44%和12.76%,2020年8月至12月为检出高峰;CoxA10型阳性率只在2020年和2021年有检出,分别为4.00%、7.23%。结论除2020年HFMD流行月份整体延后外,其余年份流行特征类似于往年。2020—2022年丽水市HFMD的主要肠道病毒型别为CoxA16和CoxA6型,期间CoxA6型的优势已逐步被CoxA16取代。CoxA16为下一步防控工作的重点。

牛碱性成纤维细胞生长因子联合盐酸金霉素眼膏对正颌外科术后口角黏膜压力性损伤愈合的效果

目的:研究外用牛碱性成纤维细胞生长因子联合盐酸金霉素眼膏用于正颌外科术后口角黏膜压力性损伤愈合的临床效果。方法:选取温州医科大学附属口腔医院2018年6月—2023年9月行正颌外科术后发生口角黏膜压力性损伤的患者为研究对象,根据用药方式不同,将患者分为盐酸金霉素眼膏组(30例)、外用牛碱性成纤维细胞生长因子组(30例)和外用牛碱性成纤维细胞生长因子联合盐酸金霉素眼膏组(简称联合组)(30例)。比较3组患者的疼痛程度、张口度、愈合时间及满意度。结果:用药第5天,3组VAS评分均较用药前显著下降(P<0.05),张口度均较用药前明显增大(P<0.05)。用药第5天,3组VAS评分和张口度差异有统计学意义(H=13.921,P=0.001;F=8.569,P<0.001),其中与金霉素眼膏和外用牛碱性成纤维细胞生长因子组相比,联合组的VAS评分最低、张口度最大(P<0.05)。3组用药前后VAS评分和张口度的差值差异有统计学意义(H=18.292,P<0.001;F=6.720,P=0.002),且联合组的差值均为最大。3组愈合时间和满意度差异均有统计学意义(F=9.510,P<0.001;χ^(2)=8.940,P=0.011),其中联合组的愈合时间最短、满意度最高(P<0.05)。结论:外用牛碱性成纤维细胞生长因子联合盐酸金霉素眼膏可促进正颌外科术后口角黏膜压力性损伤的愈合,减轻术后疼痛,增大张口度,缩短愈合时间,从而提高患者的满意度。

基于复杂网络的在线口碑传播模型研究

在线口碑已成为信息传播领域的核心力量,对其进行系统分析对于理解当代社交媒体环境中的消费者行为至关重要。论文引入了传播激活和接受激活的概念,基于病毒传播的SIR模型构建在线口碑传播模型,并运用复杂网络和系统仿真的方法模拟在线口碑传播过程,深入探讨了不同因素对在线口碑传播规模的影响,识别了在线口碑传播网络中各个节点的特征并分析了网络的结构特性。结果表明,不同因素对在线口碑传播规模的作用不同,且关键节点和免疫节点具有不同的属性特征。此外,研究还发现在线口碑传播网络具有小世界特性和无标度特性,该结论进一步丰富了在线口碑传播的理论框架,也为企业网络营销策略及产品质量追溯的优化等提供一定的应用参考。

珠江口盆地开平南大型深水深层油田发现与认识创新

开平凹陷是珠江口盆地地质条件最复杂的凹陷之一,也是重要的深水油气勘探区,由于洼陷结构、烃源分布及成藏规律认识不清,长期制约勘探进程。基于三维地震资料精细解释,开展成盆机制、烃源发育、源汇体系及成藏主控因素等研究,近期在开平南获得了大型轻质油田勘探发现。为进一步指导勘探,分析了开平凹陷油气成藏条件,总结了油气成藏模式。结果表明:(1)开平凹陷是南海北部强伸展陆缘背景下发育的“拆离-核杂岩”型凹陷,裂陷期经历“断陷—拆离—断拗”3幕演化过程;(2)开平凹陷发育文昌组优质湖相烃源岩,分布范围广、生烃潜力大,总体处于成熟至高成熟阶段;(3)开平凹陷油气运移受“源-断-脊”耦合控制,开平凹陷南部3大鼻状构造带是油气有利汇聚区,总体具有“近源强势充注-断脊差异运聚-晚期快速成藏”的模式。开平南古近系大型轻质油田的发现实现了开平凹陷勘探重大突破,开辟了珠江口盆地深水区油气勘探的新区、新领域,同时为“拆离-核杂岩”型含油气盆地的勘探提供了借鉴。

一起疱疹性咽峡炎混合手足口病疫情持续传播调查分析

目的明确深圳市罗湖区某幼儿园一起疱疹性咽峡炎(herpangina,HA)混合手足口病(hand,foot and mouth disease,HFMD)聚集性疫情的流行病学特征,分析疫情持续传播的因素,为制定有效的防控措施提供科学依据。方法应用现场流行病学方法整理分析疫情相关资料,在疫情接报和持续传播阶段分别采集病例咽拭子、肛拭子以及环境样本检测肠道病毒核酸及基因分型。结果本起疫情共持续42d,报告115例病例,无重症、住院及死亡病例。其中HA 86例,HFMD 11例,其他18例。幼儿园整体罹患率为20.7%(115/555),其中教学楼二楼儿童罹患率为27.5%(57/207),与一楼相比差异具有统计学意义(RR=1.824,95%CI:1.187~2.804)。实验室检测发现前后两次采样的病原体型别不同。疫情持续传播可能与社区密切接触、首诊误诊、晨检不规范、游园活动等因素有关。结论这是一起由多个传播源头引起的HA混合HFMD聚集性疫情。既有社区到幼儿园的传播,也存在幼儿园跨班级间传播。建议校园传染病防控增加居家隔离观察期,以防疫情持续传播。

2018年盐城市CVA6型肠道病毒全基因组分子特征分析

目的掌握盐城市肠道病毒CVA6型基因进化特征。方法对盐城市鉴定为CVA6型的肠道病毒,通过反转录聚合酶链式反应的方法扩增全基因组序列,扩增产物经纯化测序,采用相应的生物信息软件进行核苷酸及氨基酸序列比对、分子进化树构建以及重组分析。结果盐城市2018年共检测疑似疱疹性咽峡炎病例107例,肠道病毒核酸阳性率73.83%,其中CVA6、CVA16、其他肠道病毒分别占50.63%、5.06%、44.30%,未检出EV71。选取3株2018年疱疹性咽峡炎、3株2018年手足口病(流行月峰前、峰中、峰后)、1株2017年手足口病CVA6型代表株进行全基因组测序,遗传进化树显示:7株盐城株位于B进化分支B2基因亚群分支,3株疱疹性咽峡炎CVA6型代表株与2株2018年手足口病CVA6型毒株聚集成簇构成一进化小分支,2株手足口病毒株(2017年、2018年各1株)形成独立的进化小分支,形成2条传播链。选取2个进化小分支中疱疹性咽峡炎、手足口病CVA6型各1株,进行分子进化重组分析,均未发现明显的重组来源。结论2018年盐城市疱疹性咽峡炎的病原谱呈现遗传多样性,CVA6型肠道病毒为优势流行株,与手足口病的CVA6型肠道病毒可能有着共同的来源,盐城株未发现基因重组。

手足口病患儿胃生长激素释放素水平与TLR4信号通路的相关性研究

目的 分析手足口病患儿胃生长激素释放素水平与Toll样受体(TLR)4信号通路的相关性,并探讨胃生长激素释放素在手足口病炎症免疫反应中的作用机制。方法 回顾性分析100例手足口病患儿的临床资料,根据病情将其分为轻症组(n=65)和重症组(n=35),另选择100例健康儿童作为对照组(n=100)。比较三组胃生长激素释放素水平、TLR4阳性表达率和平均荧光强度(MFI),应用线性相关分析探究胃生长激素释放素水平与TLR4阳性表达率、MFI的相关性。结果 三组胃生长激素释放素水平、TLR4阳性表达率和MFI差异均有统计学意义(均P<0.05)。在胃生长激素释放素水平方面,对照组>轻症组>重症组;在TLR4阳性表达率和MFI方面,重症组>轻症组>对照组(均P<0.05)。线性相关分析结果显示,手足口病患儿胃生长激素释放素水平与TLR4阳性表达率(r=-0.729)、MFI(r=-0.697)均呈负相关(均P<0.05)。结论 手足口病患儿的胃生长激素释放素水平下降,TLR4阳性表达率和MFI升高,且胃生长激素释放素水平与TLR4阳性表达率、MFI均呈负相关。胃生长激素释放素可能在手足口病的发病机制炎症免疫反应中起到保护作用,具有抑制TLR4信号通路的作用。