目的探讨下调人类表皮生长因子受体2(HER2)磷酸化水平对骨肉瘤细胞U2-OS体外增殖、侵袭转移能力的影响。方法采用不同浓度(5、10、20、30、40μmol/L)的HER2磷酸化抑制剂二甲苯磺酸拉帕替尼作用骨肉瘤细胞U2-OS。四甲基偶氮唑盐(MTT)检...目的探讨下调人类表皮生长因子受体2(HER2)磷酸化水平对骨肉瘤细胞U2-OS体外增殖、侵袭转移能力的影响。方法采用不同浓度(5、10、20、30、40μmol/L)的HER2磷酸化抑制剂二甲苯磺酸拉帕替尼作用骨肉瘤细胞U2-OS。四甲基偶氮唑盐(MTT)检测作用不同时间(24、48、72 h)细胞的增殖能力,并计算出24 h药物的半数抑制浓度(IC50)。10μmol/L二甲苯磺酸拉帕替尼作用U2-OS细胞,Western blot检测磷酸化HER2(p-HER2)蛋白表达水平;Wound healing和Transwell invasion实验检测细胞迁徙、侵袭能力。结果二甲苯磺酸拉帕替尼显著抑制U2-OS细胞的增殖,并且呈时间、剂量依赖性;二甲苯磺酸拉帕替尼作用24 h后,细胞中p-HER2蛋白表达水平显著低于阴性对照组(0.093±0.033 vs 0.306±0.033);细胞迁徙率低于阴性对照组(%:32.70±3.00 vs 94.52±4.76),穿膜细胞数低于阴性对照组(个/视野:37±5 vs 85±10)。结论体外下调U2-OS细胞中HER2磷酸化水平能显著抑制U2-OS细胞的增殖、迁徙和侵袭,HER2有望成为抗骨肉瘤侵袭转移的分子靶点。展开更多
目的研究浅蓝菌素(cerulenin)对人成骨肉瘤细胞U2-OS在裸鼠体内生长抑制作用。方法建立人成骨肉瘤细胞U2-OS BALB/C裸鼠瘤模型。18只成瘤裸鼠按随机数字表分成3组,每组6只;对照组(DMSO溶剂)、低剂量cerulenin组(0.2 mL cerulenin配制液4...目的研究浅蓝菌素(cerulenin)对人成骨肉瘤细胞U2-OS在裸鼠体内生长抑制作用。方法建立人成骨肉瘤细胞U2-OS BALB/C裸鼠瘤模型。18只成瘤裸鼠按随机数字表分成3组,每组6只;对照组(DMSO溶剂)、低剂量cerulenin组(0.2 mL cerulenin配制液40 mg.kg-1.次-1)、高剂量cerulenin组(0.2 mL cerulenin配制液80 mg.kg-1.次-1)于腹腔隔日注射连续6次。动态观察3组肿瘤体积及裸鼠质量变化,用药结束后18 d处死裸鼠,测量瘤重、计算瘤重抑制率,测量肿瘤体积、计算肿瘤体积抑制率。肿瘤组织行形态学观察。TUNEL法检测肿瘤组织细胞凋亡情况。结果高、低剂量cerulenin组肿瘤体积抑制率分别为51.2%和24.2%,瘤重抑制率分别为49.1%和25.5%。各组间瘤体体积与瘤重比较,差异均有统计学意义(P<0.05)。瘤体组织学显示cerulenin组较对照组出现明显的细胞凋亡的超微结构改变,瘤细胞体积较小,多数细胞微绒毛消失,胞质密度增高。TUNEL法显示高、低剂量cerulenin组,对照组癌细胞凋亡率为(17.1±1.2)%、(10.3±0.8)%、(3.5±0.5)%,3组比较差异均有统计学意义(P<0.05)。结论 cerulenin能够有效诱导人成骨肉瘤细胞U2-OS在裸鼠体内凋亡,抑制肿瘤的生长。展开更多
The ab initio generalized gradient approximation (GGA)+U study of multiferroic (La Bi )<sub>2</sub>FeCrO<sub>6</sub> in pnma structure and ferri-magnetic order, including Hubbard corrections ( ...The ab initio generalized gradient approximation (GGA)+U study of multiferroic (La Bi )<sub>2</sub>FeCrO<sub>6</sub> in pnma structure and ferri-magnetic order, including Hubbard corrections ( eV) for transition metal/rare earth d-electrons with 20 atoms cell, shows optimum local magnetic moments of (Cr , Fe equal to (−2.56, 4.14) μB and an ideal spin-down band gap of 1.54 eV. Tuned-band gap La-substituted double oxide perovskites BFCO should exhibit enhanced visible-light absorption and carrier mobility, thus could be convenient light absorbers and then efficient alternatives to wide-gap chalcopyrite absorber-based solar cells failing to achieve highest power conversion efficiencies, and even compete with their metal-organic halide perovskites counterparts.展开更多
We have studied the expression of a subset of genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well as their hypoxic regulation. It was sho...We have studied the expression of a subset of genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well as their hypoxic regulation. It was shown that the expression levels of activating transcription factor 6 (ATF6), clusterin (CLU), adhesion G protein-coupled receptor E5 (ADGRE5), transglutaminase?2, C polypeptide (TGM2), leukemia inhibitory factor (LIF), phosphoserine aminotransferase 1 (PSAT1), glyoxalase I (GLO1) and tetraspanin 13 (TSPAN13) are significantly down-regulated in glioma cells with the knockdown of IRE1 signaling enzyme. It was also shown that in glioma cells subjected to hypoxia, the expression levels of PSAT1, TSPAN13, EIF2AK3, and TGM2 genes were up-regulated, whereas the expression of ATF6 gene was down-regulated. At the same time, the expression levels of LIF, CLU, and ADGRE5 genes did not change in response to hypoxic treatment.?Furthermore, inhibition of IRE1, a key effector of an unfolded protein response pathway, modified the effect of hypoxia on the expression of most studied genes. Present study demonstrates that IRE1 knockdown down-regulated the expression of most studied genes and modified their hypoxic regulation and that these changes possibly contributed to the suppression of glioma growth in cells without IRE1 signaling enzyme function.展开更多
文摘目的探讨下调人类表皮生长因子受体2(HER2)磷酸化水平对骨肉瘤细胞U2-OS体外增殖、侵袭转移能力的影响。方法采用不同浓度(5、10、20、30、40μmol/L)的HER2磷酸化抑制剂二甲苯磺酸拉帕替尼作用骨肉瘤细胞U2-OS。四甲基偶氮唑盐(MTT)检测作用不同时间(24、48、72 h)细胞的增殖能力,并计算出24 h药物的半数抑制浓度(IC50)。10μmol/L二甲苯磺酸拉帕替尼作用U2-OS细胞,Western blot检测磷酸化HER2(p-HER2)蛋白表达水平;Wound healing和Transwell invasion实验检测细胞迁徙、侵袭能力。结果二甲苯磺酸拉帕替尼显著抑制U2-OS细胞的增殖,并且呈时间、剂量依赖性;二甲苯磺酸拉帕替尼作用24 h后,细胞中p-HER2蛋白表达水平显著低于阴性对照组(0.093±0.033 vs 0.306±0.033);细胞迁徙率低于阴性对照组(%:32.70±3.00 vs 94.52±4.76),穿膜细胞数低于阴性对照组(个/视野:37±5 vs 85±10)。结论体外下调U2-OS细胞中HER2磷酸化水平能显著抑制U2-OS细胞的增殖、迁徙和侵袭,HER2有望成为抗骨肉瘤侵袭转移的分子靶点。
文摘目的研究浅蓝菌素(cerulenin)对人成骨肉瘤细胞U2-OS在裸鼠体内生长抑制作用。方法建立人成骨肉瘤细胞U2-OS BALB/C裸鼠瘤模型。18只成瘤裸鼠按随机数字表分成3组,每组6只;对照组(DMSO溶剂)、低剂量cerulenin组(0.2 mL cerulenin配制液40 mg.kg-1.次-1)、高剂量cerulenin组(0.2 mL cerulenin配制液80 mg.kg-1.次-1)于腹腔隔日注射连续6次。动态观察3组肿瘤体积及裸鼠质量变化,用药结束后18 d处死裸鼠,测量瘤重、计算瘤重抑制率,测量肿瘤体积、计算肿瘤体积抑制率。肿瘤组织行形态学观察。TUNEL法检测肿瘤组织细胞凋亡情况。结果高、低剂量cerulenin组肿瘤体积抑制率分别为51.2%和24.2%,瘤重抑制率分别为49.1%和25.5%。各组间瘤体体积与瘤重比较,差异均有统计学意义(P<0.05)。瘤体组织学显示cerulenin组较对照组出现明显的细胞凋亡的超微结构改变,瘤细胞体积较小,多数细胞微绒毛消失,胞质密度增高。TUNEL法显示高、低剂量cerulenin组,对照组癌细胞凋亡率为(17.1±1.2)%、(10.3±0.8)%、(3.5±0.5)%,3组比较差异均有统计学意义(P<0.05)。结论 cerulenin能够有效诱导人成骨肉瘤细胞U2-OS在裸鼠体内凋亡,抑制肿瘤的生长。
文摘The ab initio generalized gradient approximation (GGA)+U study of multiferroic (La Bi )<sub>2</sub>FeCrO<sub>6</sub> in pnma structure and ferri-magnetic order, including Hubbard corrections ( eV) for transition metal/rare earth d-electrons with 20 atoms cell, shows optimum local magnetic moments of (Cr , Fe equal to (−2.56, 4.14) μB and an ideal spin-down band gap of 1.54 eV. Tuned-band gap La-substituted double oxide perovskites BFCO should exhibit enhanced visible-light absorption and carrier mobility, thus could be convenient light absorbers and then efficient alternatives to wide-gap chalcopyrite absorber-based solar cells failing to achieve highest power conversion efficiencies, and even compete with their metal-organic halide perovskites counterparts.
文摘We have studied the expression of a subset of genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well as their hypoxic regulation. It was shown that the expression levels of activating transcription factor 6 (ATF6), clusterin (CLU), adhesion G protein-coupled receptor E5 (ADGRE5), transglutaminase?2, C polypeptide (TGM2), leukemia inhibitory factor (LIF), phosphoserine aminotransferase 1 (PSAT1), glyoxalase I (GLO1) and tetraspanin 13 (TSPAN13) are significantly down-regulated in glioma cells with the knockdown of IRE1 signaling enzyme. It was also shown that in glioma cells subjected to hypoxia, the expression levels of PSAT1, TSPAN13, EIF2AK3, and TGM2 genes were up-regulated, whereas the expression of ATF6 gene was down-regulated. At the same time, the expression levels of LIF, CLU, and ADGRE5 genes did not change in response to hypoxic treatment.?Furthermore, inhibition of IRE1, a key effector of an unfolded protein response pathway, modified the effect of hypoxia on the expression of most studied genes. Present study demonstrates that IRE1 knockdown down-regulated the expression of most studied genes and modified their hypoxic regulation and that these changes possibly contributed to the suppression of glioma growth in cells without IRE1 signaling enzyme function.
