目的:研究泛肽相关新基因编码产物的特性和在鼻咽癌细胞内定位与表达。方法:利用生物信息学分析编码蛋白的一般性质并预测其定位,构建增强型绿色荧光蛋白(enhance green fluorescent prote in,EGFP)与UBAP1融合基因的真核表达载体pEGFP-...目的:研究泛肽相关新基因编码产物的特性和在鼻咽癌细胞内定位与表达。方法:利用生物信息学分析编码蛋白的一般性质并预测其定位,构建增强型绿色荧光蛋白(enhance green fluorescent prote in,EGFP)与UBAP1融合基因的真核表达载体pEGFP-C2-UBAP1,通过脂质体介导转染人鼻咽癌细胞系HNE1,荧光显微镜观察UBAP1基因编码蛋白在HNE1活细胞内定位。结果:融合蛋白可在HNE1细胞中高表达,主要定位在细胞核,有较为明显的核膜聚集现象。结论:UBAP1基因编码产物在HNE1中的表达差异可能是NPC发生的原因之一。展开更多
Objective: To identify the relation between nasopharyngeal carcinoma and the human novel gene UBAP1, which is located in the region of minimal heterozygosity deletion at 9pl3.2 and down-expressed in NPC. Methods: Fiv...Objective: To identify the relation between nasopharyngeal carcinoma and the human novel gene UBAP1, which is located in the region of minimal heterozygosity deletion at 9pl3.2 and down-expressed in NPC. Methods: Five single nucleotide polymorphisms (SNPs) within UBAP1 gene were analysed by sequencing in 105 NPC patients and 183 control subjects which matched to the NPC cases on age, sex and residence. Results: Significant association was found between NPC with one SNP mark (rs1049557), which is located at 3' non-region of UBAP1 gene; the relative risk of this SNP mark is 1.64 (genotype GG) and 1.31 (genotype CG). Conclusion: The result has proved again that UBAP1 gene may play a certain role in the occurrence and development of nasopharyngeal carcinoma. The SNP mark rs1049557, considering its location, may influence the expression of UBAP1 gene.展开更多
Objective: To identify the differential expression profile of human novel gene UBAP1, a putative nasopharyngeal neoplasms (NPC) relate gene, in multiple cancers. Methods: We first present an EST approach for electroni...Objective: To identify the differential expression profile of human novel gene UBAP1, a putative nasopharyngeal neoplasms (NPC) relate gene, in multiple cancers. Methods: We first present an EST approach for electronic Northern in silico to analyse expression patterns of UBAP1 in tumor and normal tissues. Full length cDNA of UBAP1 gene was taken as a “probe” sequence, and a blastn search was performed against human EST Database. The Blastn report can be used to determine the fold differences between the pedigree ESTs in different libraries. Especially, the ESTs corresponding to UBAP1 present in fifteen tumor-derived libraries were compared against their normal counterpart to produce an electronic differential expression profile. Second, the distinct down-regulation of UBAP1 in meningioma, glioma, and colorectal tumor was confirmed by differentially RT-PCR analysis. Results: Database surveys indicated that UBAP1 gene was not only ubiquitously expressed in many normal tissues with various levels but also differentially expressed in different tumor tissues, especially down-regulated in multiple neoplastic tissues such as brain, breast, skin, colon, testis and uterus tumor tissues. Furthermore, differential RT-PCR analysis demonstrated that expression of UBAP1 was down-regulated or absent in 7 of 12 (58%) meningioma samples, 6 of 9 (66%) glioma and 7 of 11 (63%) colorectal tumor tissues respectively. Conclusion: we described a data mining procedure in silico that proved to be useful for the identification of differential expression patterns of UBAP1. These findings could be valuable for the investigation of the mechanism the differential expression of UBAP1 gene and its significance in the progression of multiple cancers.展开更多
文摘目的:研究泛肽相关新基因编码产物的特性和在鼻咽癌细胞内定位与表达。方法:利用生物信息学分析编码蛋白的一般性质并预测其定位,构建增强型绿色荧光蛋白(enhance green fluorescent prote in,EGFP)与UBAP1融合基因的真核表达载体pEGFP-C2-UBAP1,通过脂质体介导转染人鼻咽癌细胞系HNE1,荧光显微镜观察UBAP1基因编码蛋白在HNE1活细胞内定位。结果:融合蛋白可在HNE1细胞中高表达,主要定位在细胞核,有较为明显的核膜聚集现象。结论:UBAP1基因编码产物在HNE1中的表达差异可能是NPC发生的原因之一。
基金This work was supported by a grant from the National 63High-Tech Program of China (No. 2001AA221031) and a grant from the National Natural Science Foundation of China (No.30100027). *Author to whom correspondence should be addressed.
文摘Objective: To identify the relation between nasopharyngeal carcinoma and the human novel gene UBAP1, which is located in the region of minimal heterozygosity deletion at 9pl3.2 and down-expressed in NPC. Methods: Five single nucleotide polymorphisms (SNPs) within UBAP1 gene were analysed by sequencing in 105 NPC patients and 183 control subjects which matched to the NPC cases on age, sex and residence. Results: Significant association was found between NPC with one SNP mark (rs1049557), which is located at 3' non-region of UBAP1 gene; the relative risk of this SNP mark is 1.64 (genotype GG) and 1.31 (genotype CG). Conclusion: The result has proved again that UBAP1 gene may play a certain role in the occurrence and development of nasopharyngeal carcinoma. The SNP mark rs1049557, considering its location, may influence the expression of UBAP1 gene.
基金supported by a grant from the National"863"High-Tech Program of China(Nos.102-10-01-05,Z19-01-01-03)the National"973"Key Basic Research Program of China(Fundamental Investigation on HumanCarcinogenesis)(No.G1998051008)and a grant from Chinese Medicine Board of New York,Inc.(No.96655)
文摘Objective: To identify the differential expression profile of human novel gene UBAP1, a putative nasopharyngeal neoplasms (NPC) relate gene, in multiple cancers. Methods: We first present an EST approach for electronic Northern in silico to analyse expression patterns of UBAP1 in tumor and normal tissues. Full length cDNA of UBAP1 gene was taken as a “probe” sequence, and a blastn search was performed against human EST Database. The Blastn report can be used to determine the fold differences between the pedigree ESTs in different libraries. Especially, the ESTs corresponding to UBAP1 present in fifteen tumor-derived libraries were compared against their normal counterpart to produce an electronic differential expression profile. Second, the distinct down-regulation of UBAP1 in meningioma, glioma, and colorectal tumor was confirmed by differentially RT-PCR analysis. Results: Database surveys indicated that UBAP1 gene was not only ubiquitously expressed in many normal tissues with various levels but also differentially expressed in different tumor tissues, especially down-regulated in multiple neoplastic tissues such as brain, breast, skin, colon, testis and uterus tumor tissues. Furthermore, differential RT-PCR analysis demonstrated that expression of UBAP1 was down-regulated or absent in 7 of 12 (58%) meningioma samples, 6 of 9 (66%) glioma and 7 of 11 (63%) colorectal tumor tissues respectively. Conclusion: we described a data mining procedure in silico that proved to be useful for the identification of differential expression patterns of UBAP1. These findings could be valuable for the investigation of the mechanism the differential expression of UBAP1 gene and its significance in the progression of multiple cancers.
