AIM: To explore the genetic diversities of UL144 open reading frame (ORF) of cytomegalovirus DNA detected in colon tissue from infants with Hirschsprung's disease (HD) by sequencing UL144 DNA in 23 aganglionic c...AIM: To explore the genetic diversities of UL144 open reading frame (ORF) of cytomegalovirus DNA detected in colon tissue from infants with Hirschsprung's disease (HD) by sequencing UL144 DNA in 23 aganglionic colon tissue and 4 urine samples from 25 HD infants. METHODS: Nest PCR was performed for amplification of the UL144 gene. The UL144 gene was analyzed with soffwares, such as DNAclub, BioEdit, PROSITE database, and DNAstar. RESULTS: The strains from HD patients were distributed among three genotypes of UL144: group 1A (64%), group 2 (24%), and group 3 (12%). The UL144 genotypes between strains from HD and control group were compared by chi square test (x^2 = 1.870, P = 0.393). Strains from the colon were sporadically distributed in UL144 genotypes. CONCLUSION: There are genetic diversities of UL144 ORF in colon tissue of infants with HD. However, cytomegalovirus UL144 genotypes are not associated with clinical manifestations of HD.展开更多
To explore the impact of gene polymorphism of human cytomegalovirus(HCMV)on virus virulence,the full-length UL144 gene and partial sequence of glycoprotein B(UL55)gene were sequenced and analyzed for 23clinical strain...To explore the impact of gene polymorphism of human cytomegalovirus(HCMV)on virus virulence,the full-length UL144 gene and partial sequence of glycoprotein B(UL55)gene were sequenced and analyzed for 23clinical strains of HCMV isolated from urine samples of pediatric patients with congenital or postnatal HCMV infection.Among the 23 isolates,13(57%)were UL144genotype 1A,3(13%)were UL144 genotypes 2 and 7(30%)were UL144 genotype 3;geographic differences in genotype distribution were found for both UL144 and gB gene.No UL144 genotypes 1B and 1C were found in this study while these two genotypes were common in HCMV strains isolated in the US.Our results also demonstrated that for all clinical strains of gB genotypes I,III and UL144genotypes 1A,2,and 3 found in this study,mother-to-fetus vertical transmission was possible.展开更多
基金Supported by the National Natural Science Foundation of China, No.30170986
文摘AIM: To explore the genetic diversities of UL144 open reading frame (ORF) of cytomegalovirus DNA detected in colon tissue from infants with Hirschsprung's disease (HD) by sequencing UL144 DNA in 23 aganglionic colon tissue and 4 urine samples from 25 HD infants. METHODS: Nest PCR was performed for amplification of the UL144 gene. The UL144 gene was analyzed with soffwares, such as DNAclub, BioEdit, PROSITE database, and DNAstar. RESULTS: The strains from HD patients were distributed among three genotypes of UL144: group 1A (64%), group 2 (24%), and group 3 (12%). The UL144 genotypes between strains from HD and control group were compared by chi square test (x^2 = 1.870, P = 0.393). Strains from the colon were sporadically distributed in UL144 genotypes. CONCLUSION: There are genetic diversities of UL144 ORF in colon tissue of infants with HD. However, cytomegalovirus UL144 genotypes are not associated with clinical manifestations of HD.
基金supported by the Innovation Platform Open Funds for Universities in Hunan Province(09K022and 11K010)the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province
文摘To explore the impact of gene polymorphism of human cytomegalovirus(HCMV)on virus virulence,the full-length UL144 gene and partial sequence of glycoprotein B(UL55)gene were sequenced and analyzed for 23clinical strains of HCMV isolated from urine samples of pediatric patients with congenital or postnatal HCMV infection.Among the 23 isolates,13(57%)were UL144genotype 1A,3(13%)were UL144 genotypes 2 and 7(30%)were UL144 genotype 3;geographic differences in genotype distribution were found for both UL144 and gB gene.No UL144 genotypes 1B and 1C were found in this study while these two genotypes were common in HCMV strains isolated in the US.Our results also demonstrated that for all clinical strains of gB genotypes I,III and UL144genotypes 1A,2,and 3 found in this study,mother-to-fetus vertical transmission was possible.