Molecular Characterization of the Duck Enteritis Virus UL4 Gene

Duck enteritis virus(DEV) is a herpesvirus that causes an acute,contagious and fatal disease. In the present article,the DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer for the consensus site of herpesvirus UL3 gene and a specific primer located in UL5 were used in the polymerase chain reaction(PCR) to amplify a DNA product 2 086 bp in size. DNA sequence analysis revealed that a 714 bp open reading frame(ORF) of DEV encoding a 237 amino acid polypeptide is homologous to the family of herpesvirus UL4 proteins and therefore has been characterized as a DEV UL4 gene. Alignment of the DEV UL4 protein sequence with those of other alphaherpesviruses showed that 10 amino acid residues are completely conserved. Phylogenetic tree analysis showed that the seventeen alphaherpesviruses viruses analyzed were classified into four large groups,and the duck enteritis virus branched separately,closely related to the Mardiviruses group comprising Gallid herpesvirus 2(GaHV-2) ,Gallid herpesvirus 3(GaHV-3) and Meleagrid herpesvirus 1(MeHV-1) . The present study showed that the evolutionary relationship of the UL4 protein could be used for classification of alphaherpesviruses.

Molecular Determinants Responsible for the Subcellular Localization of HSV-1 UL4 Protein

The function of the herpes simplex virus type 1 （HSV-1） UL4 protein is still elusive. Our objective is to investigate the subcellular transport mechanism of the UL4 protein. In this study, fluorescence microscopy was employed to investigate the subcellular localization of UL4 and characterize the transport mechanism in living cells. By constructing a series of deletion mutants fused with enhanced yellow fluorescent protein （EYFP）, the nuclear export signals （NES） of UL4 were for the first time mapped to amino acid residues 178 to 186. In addition, the N-terminal 19 amino acids are identified to be required for the granule-like cytoplasmic pattem of UL4. Furthermore, the UL4 protein was demonstrated to be exported to the cytoplasm through the NES in a chromosomal region maintenance 1 （CRM1）-dependent manner involving RanGTP hydrolysis

锐劲特4UL防治草地蝗虫药效研究

通过在宁夏中卫县干草原以马拉硫磷为对照所作的试验可得出如下结论：（１）锐劲特４ＵＬ同马拉硫磷一样是一种高效草原灭蝗剂．（２）它有两大优点：长效作用优于马拉硫磷；有效成份仅４ｇ／Ｌ，每ｈｍ２只需４ｇ有效成份，毒性小．（３）４ｇ／ｈｍ２的药量效果最好．（４）考虑防治成本，２ｇ／ｈｍ２也能达到比较理想的防治效果．

UL 44铝合金电缆紧压导体设计和制造

文中就UL 44铝合金电缆Compact导体要求作了介绍,叙述了用常规工艺生产符合型线绞合Compact导体要求的设计和制造。

人参皂苷Rg3对宫颈癌U14细胞凋亡水平影响的实验研究

目的 探讨人参皂苷Rg3对宫颈癌U14细胞凋亡水平的影响。方法 10%小牛血清的DMEM营养液培养宫颈癌U14细胞,待细胞铺满培养板底80%~90%时分为对照组、Rg3低剂量组（20μg/ml）、Rg3中剂量组（40μg/ml）、Rg3高剂量组（80μg/ml）和环磷酰胺阳性药组（50μg/ml）开始加药,培养24 h后终止培养。通过CCK-8法检测细胞增殖活性,倒置显微镜观察细胞生长状态及免疫细胞化学法检测细胞凋亡状况。结果 倒置显微镜观察可见随剂量增大U14细胞排列逐渐松散、萎缩,细胞漂浮死亡数量增多;CCK-8法检测显示Rg3低、中、高剂量组及阳性药组对U14细胞的增殖活性均有抑制作用,免疫细胞化学法显示Rg3低、中、高剂量组均能促进细胞凋亡,组间差异均有统计学意义（P〈0.01）,且阳性药组与高剂量组差异无统计学意义（P〉0.05）。结论 Rg3能明显抑制U14细胞生长,通过下调Survivin和Bcl-2基因、上调Caspase-3和Bax基因诱导其凋亡,从而发挥抑瘤作用。