WTFZ] The study of the bioeffects of electromagnetic fields (EMFs) is an important national task in biological physics. Using EMFs to treat bone diseases involves electrical technology, biology, and medicine. But the...WTFZ] The study of the bioeffects of electromagnetic fields (EMFs) is an important national task in biological physics. Using EMFs to treat bone diseases involves electrical technology, biology, and medicine. But the effects of EMFs are still controversial and the mechanisms are not yet clear. Therefore, more effect is needed to detect the effects at the cellular and molecular levels. This paper investigates the effects of low-energy, low-frequency pulsed capacitively coupled electric fields (PCCEFs) on DNA synthesis in UMR-106 osteoblast-like cells. The equipment can generate 25250Hz frequency, 0300V amplitude and 0.2ms pulse width signal. DNA synthesis is judged by the uptake of 3 H-thymidine ( 3 H-TdR). The results showed that the response of UMR-106 cells to electric field exposure are characterized by: (a) a frequency window for increased DNA synthesis, with a peak near 125Hz; (b) decreased synthesis with increasing electric intensity with repression at 100V/cm and 25Hz.[展开更多
目的 探讨氟化钠（Na F）对成骨样细胞大鼠骨肉瘤（UMR-106）细胞增殖活性及主要成骨活性标志物碱性磷酸酶（ALP）活力和骨钙素（BGP）m RNA表达的影响。方法 将处于对数生长期的UMR-106细胞分别暴露于含终浓度为0（对照）、5×10、...目的 探讨氟化钠（Na F）对成骨样细胞大鼠骨肉瘤（UMR-106）细胞增殖活性及主要成骨活性标志物碱性磷酸酶（ALP）活力和骨钙素（BGP）m RNA表达的影响。方法 将处于对数生长期的UMR-106细胞分别暴露于含终浓度为0（对照）、5×10、102、5×102、1×103、5×103、1×104、2×104、4×104、8×104μmol/L Na F的DMEM培养液中孵育24、48 h,采用CCK-8检测细胞活性。将处于对数生长期的UMR-106细胞分别暴露于含终浓度为0（对照）、1×103、2×103、4×103μmol/L Na F的DMEM培养液（不含FBS）继续培养12、24、48 h,测定细胞培养液和细胞ALP的活力及细胞中BGP m RNA的表达水平。结果 与对照组比较,1×103~8×104μmol/L Na F染毒24 h和5×10~8×104μmol/L Na F染毒48 h时大鼠UMR-106细胞的存活率均较低,差异有统计学意义（P〈0.05）。5×10、1×103、5×103、1×104、2×104μmol/L Na F染毒48 h时大鼠UMR-106细胞的存活率均低于染毒24 h时,差异有统计学意义（P〈0.05）。各浓度Na F染毒组UMR-106细胞培养液和细胞中ALP活力均较对照组高,除1×103μmol/L Na F染毒24 h的细胞培养液和1×103μmol/L Na F染毒48 h的细胞内ALP活力外,差异有统计学意义（P〈0.05）。随着Na F染毒浓度的升高,UMR-106细胞培养液和细胞中ALP活力均呈上升趋势;随着Na F染毒时间的延长,细胞培养液中ALP的活力呈上升趋势,而细胞中ALP的活力呈先升高后下降的趋势。2×103、4×103μmol/L Na F染毒组UMR-106细胞的BGP m RNA表达水平均较对照组高,差异有统计学意义（P〈0.05）。随着Na F染毒浓度的升高,UMR-106细胞BGP m RNA的表达水平呈上升趋势;随着Na F染毒时间的延长,UMR-106细胞BGP m RNA的表达水平呈下降趋势。结论 在本实验浓度下,Na F对UMR-106细胞的增殖呈抑制作用,但可促进其成骨活性标志物ALP活力及BGP m RNA的表达。展开更多
文摘WTFZ] The study of the bioeffects of electromagnetic fields (EMFs) is an important national task in biological physics. Using EMFs to treat bone diseases involves electrical technology, biology, and medicine. But the effects of EMFs are still controversial and the mechanisms are not yet clear. Therefore, more effect is needed to detect the effects at the cellular and molecular levels. This paper investigates the effects of low-energy, low-frequency pulsed capacitively coupled electric fields (PCCEFs) on DNA synthesis in UMR-106 osteoblast-like cells. The equipment can generate 25250Hz frequency, 0300V amplitude and 0.2ms pulse width signal. DNA synthesis is judged by the uptake of 3 H-thymidine ( 3 H-TdR). The results showed that the response of UMR-106 cells to electric field exposure are characterized by: (a) a frequency window for increased DNA synthesis, with a peak near 125Hz; (b) decreased synthesis with increasing electric intensity with repression at 100V/cm and 25Hz.[
文摘目的 探讨氟化钠（Na F）对成骨样细胞大鼠骨肉瘤（UMR-106）细胞增殖活性及主要成骨活性标志物碱性磷酸酶（ALP）活力和骨钙素（BGP）m RNA表达的影响。方法 将处于对数生长期的UMR-106细胞分别暴露于含终浓度为0（对照）、5×10、102、5×102、1×103、5×103、1×104、2×104、4×104、8×104μmol/L Na F的DMEM培养液中孵育24、48 h,采用CCK-8检测细胞活性。将处于对数生长期的UMR-106细胞分别暴露于含终浓度为0（对照）、1×103、2×103、4×103μmol/L Na F的DMEM培养液（不含FBS）继续培养12、24、48 h,测定细胞培养液和细胞ALP的活力及细胞中BGP m RNA的表达水平。结果 与对照组比较,1×103~8×104μmol/L Na F染毒24 h和5×10~8×104μmol/L Na F染毒48 h时大鼠UMR-106细胞的存活率均较低,差异有统计学意义（P〈0.05）。5×10、1×103、5×103、1×104、2×104μmol/L Na F染毒48 h时大鼠UMR-106细胞的存活率均低于染毒24 h时,差异有统计学意义（P〈0.05）。各浓度Na F染毒组UMR-106细胞培养液和细胞中ALP活力均较对照组高,除1×103μmol/L Na F染毒24 h的细胞培养液和1×103μmol/L Na F染毒48 h的细胞内ALP活力外,差异有统计学意义（P〈0.05）。随着Na F染毒浓度的升高,UMR-106细胞培养液和细胞中ALP活力均呈上升趋势;随着Na F染毒时间的延长,细胞培养液中ALP的活力呈上升趋势,而细胞中ALP的活力呈先升高后下降的趋势。2×103、4×103μmol/L Na F染毒组UMR-106细胞的BGP m RNA表达水平均较对照组高,差异有统计学意义（P〈0.05）。随着Na F染毒浓度的升高,UMR-106细胞BGP m RNA的表达水平呈上升趋势;随着Na F染毒时间的延长,UMR-106细胞BGP m RNA的表达水平呈下降趋势。结论 在本实验浓度下,Na F对UMR-106细胞的增殖呈抑制作用,但可促进其成骨活性标志物ALP活力及BGP m RNA的表达。
