Analytical Method Development and Validation of Filgrastim by UV and RP-UFLC Methods

The research work was carried out for establishing a new Ultra Violet (UV)— Visible spectroscopy and Reverse phase-Ultra Fast Liquid Chromatography (RP-UFLC) method for the analysis and quantification of a biosimilar drug, Filgrastim. Filgrastim or recombinant methionyl granulocyte colony stimulating factor (rGCSF) is a glycoprotein. It has a biological action essential for proliferation and differentiation of hematopoetic and progenitor cells. The UV and RP-UFLC work was carried on a Shimadzu UV1800 Spectrophotometer and Shimadzu Prominence LC-20AD UFLC systems, respectively. The λmax of filgrastim was found to be 215 nm. The correlation coefficient by UV spectroscopy was found to be 0.9994 for the concentration range of 1 to 3 μg/ml in double distilled water. The Reverse phase UFLC was done by using Phenomenex C4 (25 cm × 0.46 cm internal diameter) 15 μ, 300 A° analytical column. The optimized mobile phase for binary elution was Acetonitrile and double distilled water (80:20) with a flow rate of 1 ml/min. The retention time of drug was at 3.2 min. It was observed that the response of the detector was linear in the range of 5 - 15 μg/ml with correlation coefficient value of 0.999. After developing the methods, it was assured for the intended use by validation of the analytical parameters like linearity, accuracy, precision, limit of detection, limit of quantitation, ruggedness and robustness. The results of all the parameters for both the methods were found to be within the acceptance criteria as per the International Council for Harmonisation (ICH) guidelines.

基于紫外-可见光光谱法研究长期不同施肥对砖红壤溶解性有机质化学性质的影响

溶解性有机质(DOM)是土壤中最为活跃的一部分,具有重要的生态环境意义。明确不同施肥方式对DOM化学性质的影响,可为土壤肥力管理提供依据。因此,该试验以连续四年的不同施肥管理为基础,结合紫外-可见吸收光谱,探求不同施肥方式下,土壤中溶解性有机质的含量和化学性质的变化情况。试验共设置4个处理:CK(不施肥);CF(化肥);OG(有机肥);ST(秸秆)。结果表明,与CK组相比,OG和ST的DOC(溶解性有机碳)含量为95.97和104.89 mg·kg^(-1),分别是CK组的129%和141%。相反,CF的DOC含量为15.32 mg·kg^(-1),仅为CK组的21%。OG显著提高了有色溶解性有机质[CDOM,以α(355)表征]含量,是CK组的2.76倍,ST无明显变化;CF则显著降低CDOM含量,仅为CK组的0.55倍。施用OG导致土壤紫外吸收曲线红移,表明有机肥的施用能够提高土壤DOM的共轭双键物质含量和腐殖化程度;与CK相比,OG中DOM的芳香性、疏水性、腐殖化程度的特征常数SUVA,SUVA和SUVA增大,揭示了有机肥的施用能够提高DOM的芳香性、疏水性组分以及腐殖化程度,秸秆处理增加不明显。施用化肥则出现明显降低。吸光度比值A/A,相比CK,CF显著增加,表明施用化肥使DOM分子变小,OG和ST处理无明显变化;秸秆施用导致A/A显著增加,表明秸秆能够有效提高DOM中蛋白质和碳水化合物含量;施用有机肥和秸秆A/A均大于3.5,表明土壤中DOM主要以富里酸为主,而施用化肥A/A明显低于3.5,表明土壤中DOM主要以胡敏酸为主。施用化肥出现S>1,不施肥、有机肥、秸秆三者的S<1,也表明了有机肥、秸秆能够增加分子量。有机肥和秸秆能有效增加土壤DOM含量,提升土壤肥力。尤其是施用有机肥,更能显著增加土壤DOM的共轭物质、腐殖化程度、疏水比例、芳香性以及分子量。而长期施用化肥则导致耕层土壤肥力降低。

Spectroscopic Analysis of Expired and Pure Melmet

Spectroscopy is a perfect analyzer to find the elements of all matter. The application of spectroscopy has been used to interpret the effect of antibiotics and other medicines after its life duration. This has been studied by using the diabetics tablet melmet, expired for an year, to visualize the changes physically and chemically [1-3]. It has been observed that the occurrence of changes in color and reduction of smell and also the formation of new peaks and shift by XRD and UV, FTIR characterization respectively [4].

碳量子点双光法检测溶液中的钴离子

发现合成的碳量子点加入钴离子后溶液变成绿色,在650 nm处有紫外吸收,适用检测范围为0.8~35 mmol/L,方程式为y=0.007 46x+0.219 51,R^(2)=0.998 2;当钴离子浓度为0.04~6 mmol/L能猝灭碳量子点的荧光,浓度的对数与F_(0)/F比值呈现线性相关系数R^(2)=0.996,线性方程为:y=0.565 3x+1.739 6。因此,当钴离子浓度为0.8~6.0 mmol/L时,此碳量子点能达到双光法检测溶液中的钴离子,通过加标回收自来水样验证双光法检测有效。

部分水解聚丙烯酰胺/Cr(Ⅲ)交联作用的研究方法

部分水解聚丙烯酰胺 (HPAM) Cr(Ⅲ )凝胶已在油田广泛应用 ,是一类有效的调剖堵水剂 ,对于调整吸水剖面和提高原油采收率起到重要作用。本文简要概述HPAM Cr(Ⅲ )交联反应机理的研究方法 ,如粘度法、紫外 可见吸光光度法、原子力显微镜 (AFM)、激光光散射法、原子吸收法等 。

利用紫外－可见吸收光谱同时定量分析C＿（60）和C＿（70）

利用紫外可见吸收光谱通过卡尔曼滤波定量分析Ｃ＿６０和Ｃ＿７０法混合物中的Ｃ＿（６０）和Ｃ＿（７０）浓度，其相对误差不超过２％。

新型芳基异丙酸类化合物与牛血清白蛋白的相互作用

目的研究新型芳基异丙酸类化合物2-[4-(3-氧代丁基)苯基]丙酸(OPPA)与牛血清白蛋白(BSA)的相互作用。方法利用瞬态/稳态荧光光谱法和紫外光谱法,测算结合常数、结合位点数、热力学参数和分子的结合距离。结果在25℃下,反应平衡常数为3.9×104L·mol-1,结合位点数为0.8;焓变为-16.29 k J·mol-1,熵变为205.04 J·mol-1·K-1,吉布斯自由能为-26.21 k J·mol-1;OPPA与BSA分子中色氨酸的最近距离为3.26 nm。结论 OPPA与BSA的相互作用为动态猝灭和静态猝灭的协同机理,OPPA与BSA的相互作用力主要是疏水相互作用力和静电作用力相互作用。