The binding equilibrium between phosphotungstic acid (H7[P(W2O7)6]@XH2O;PTA) and human serum albumin (HSA) or bovine serum albumin (BSA) has been studied by UV-Vis, fluorescence spectroscopies and equilibrium dialysis...The binding equilibrium between phosphotungstic acid (H7[P(W2O7)6]@XH2O;PTA) and human serum albumin (HSA) or bovine serum albumin (BSA) has been studied by UV-Vis, fluorescence spectroscopies and equilibrium dialysis. It has been observed that UV absorption enhanced and the fluorescence quenched as the PTA binding to HSA or BSA at physiological pH 7.43( ± 0.02). The Scatchard analysis indicated that there exists a strong binding site of PTA in both HSA and BSA, and the successive stability constants of these two systems are obtained by nonlinear least-squares methods fitting Bjerrum formula.展开更多
The photoluminescence spectra and excitation spectra in the vacuum ultraviolet (VUV) and ultraviolet (UV) spectra region of GdVO 4∶Eu 3+ were reported. The excitation and emission spectra indicate that GdVO 4...The photoluminescence spectra and excitation spectra in the vacuum ultraviolet (VUV) and ultraviolet (UV) spectra region of GdVO 4∶Eu 3+ were reported. The excitation and emission spectra indicate that GdVO 4∶Eu 3+ is a highly efficient phosphor for the excitation in the VUV and UV spectrum region. The excitation band in the VUV and UV spectrum region of 60～350 nm consists mainly of the absorption of host, which has obvious absorption of 4f n-1 5d of Eu 3+ and Gd 3+ . In GdVO 4∶Eu 3+ , there is the energy transfer: VO 4 3- →Eu 3+ , Gd 3+ →Eu 3+ and Gd 3+ →VO 4 3- →Eu 3+ . Through the last two procedures, maybe the quantum cutting of Gd 3+ -Eu 3+ occurs.展开更多
UV-Vis spectrum and the third-order nonlinear optical properties of the chiral camphor-derived β-diketonate have been studied at the B3LYP/6-31G＊ level. The results showed that the introduction of electron-drawing g...UV-Vis spectrum and the third-order nonlinear optical properties of the chiral camphor-derived β-diketonate have been studied at the B3LYP/6-31G＊ level. The results showed that the introduction of electron-drawing group -CF3 and -C3F7 on β-diketonate made the strongest absorption peak red-shift and the lowest energy absorption blue-shied. Introduction of -OC2H5 on the benzene or pyridine ring made the lowest energy absorption blue-shift. When the -C2H3 was introduced on the benzene or pyridine ring, the lowest energy absorption was red-shifted. Introduction of electron-donating group on β-diketonate can enlarge their nonlinear optical properties. On the contrary, the introduction of electron-drawing group dropped it down.展开更多
Objective： The aim of our study was to discovery the different cell cycle arrest effect after different densities HeLa cells treated with Trichostatin A （TSA）. In addition, this study would find some important rela...Objective： The aim of our study was to discovery the different cell cycle arrest effect after different densities HeLa cells treated with Trichostatin A （TSA）. In addition, this study would find some important relationship between cycle arrest effect and UV absorption spectrum of cell. Methods： 0.2 IJM TSA was applied to act on HeLa cells of different density. Then, the cycle arrest effect and UV absorption spectrum of cells were investigated, which provide support to analyze the effect of TSA on cancer cells. Results： Cell cycle arrest effect in G0/G1 of the lower density cells was more obvious than that in other groups. The other discovery in this work was that the cellular UV absorption value was higher when the density of cultured cell was lower. Conclusion： This experiment would guide the clinical study on early or late stage cancer patients in the future. On the other hand, this work indicates when cells were arrested in G0/G1 phase, the cellular absorption value increased at the same time, so UV absorption spectrum could characterize the change of cell cycle.展开更多
Geometry optimization of p-C_(6)H_(4)-connected cyclo[20]carbon(p-C_(6)H_(4)-C_(20))was carried out at M062X/6-311G(d,p)level,three kinds of bond orders(Mayer,Laplacian,and Wiberg),electron-hole distributions,localize...Geometry optimization of p-C_(6)H_(4)-connected cyclo[20]carbon(p-C_(6)H_(4)-C_(20))was carried out at M062X/6-311G(d,p)level,three kinds of bond orders(Mayer,Laplacian,and Wiberg),electron-hole distributions,localized orbital locators(LOL),and infrared(IR)spectrum were also performed at the same level.Based on TD-DFT M062X/6-311G(d,p)method,the first 20 excited states and ultraviolet(UV)spectra of p-C_(6)H_(4)-C_(20) were calculated.Calculation results of π-electron delocalization analyses prove thatπ-electron delocalization of p-C_(6)H_(4)-C_(20) is more likely to occur on shorter C-C bonds rather than longer C-C bonds,and inside/outside of the ring plane rather than above/below the ring plane.Two absorption peaks of p-C_(6)H_(4)-C_(20) locate at about 319 nm and 236 nm,respectively.展开更多
基金This work was supported by the National Natural Science Foundation of China (Grant No.29961001) the Cross-Century Talents Foundation of Guangxi Zhuang Autonomous Region and Science Foundation of Guangxi University.
文摘The binding equilibrium between phosphotungstic acid (H7[P(W2O7)6]@XH2O;PTA) and human serum albumin (HSA) or bovine serum albumin (BSA) has been studied by UV-Vis, fluorescence spectroscopies and equilibrium dialysis. It has been observed that UV absorption enhanced and the fluorescence quenched as the PTA binding to HSA or BSA at physiological pH 7.43( ± 0.02). The Scatchard analysis indicated that there exists a strong binding site of PTA in both HSA and BSA, and the successive stability constants of these two systems are obtained by nonlinear least-squares methods fitting Bjerrum formula.
文摘The photoluminescence spectra and excitation spectra in the vacuum ultraviolet (VUV) and ultraviolet (UV) spectra region of GdVO 4∶Eu 3+ were reported. The excitation and emission spectra indicate that GdVO 4∶Eu 3+ is a highly efficient phosphor for the excitation in the VUV and UV spectrum region. The excitation band in the VUV and UV spectrum region of 60～350 nm consists mainly of the absorption of host, which has obvious absorption of 4f n-1 5d of Eu 3+ and Gd 3+ . In GdVO 4∶Eu 3+ , there is the energy transfer: VO 4 3- →Eu 3+ , Gd 3+ →Eu 3+ and Gd 3+ →VO 4 3- →Eu 3+ . Through the last two procedures, maybe the quantum cutting of Gd 3+ -Eu 3+ occurs.
基金supported by the National Natural Science Foundation of China(21172161)
文摘UV-Vis spectrum and the third-order nonlinear optical properties of the chiral camphor-derived β-diketonate have been studied at the B3LYP/6-31G＊ level. The results showed that the introduction of electron-drawing group -CF3 and -C3F7 on β-diketonate made the strongest absorption peak red-shift and the lowest energy absorption blue-shied. Introduction of -OC2H5 on the benzene or pyridine ring made the lowest energy absorption blue-shift. When the -C2H3 was introduced on the benzene or pyridine ring, the lowest energy absorption was red-shifted. Introduction of electron-donating group on β-diketonate can enlarge their nonlinear optical properties. On the contrary, the introduction of electron-drawing group dropped it down.
基金Supported by a grant from the Education Office of Henan Province in China (No.12A140013)
文摘Objective： The aim of our study was to discovery the different cell cycle arrest effect after different densities HeLa cells treated with Trichostatin A （TSA）. In addition, this study would find some important relationship between cycle arrest effect and UV absorption spectrum of cell. Methods： 0.2 IJM TSA was applied to act on HeLa cells of different density. Then, the cycle arrest effect and UV absorption spectrum of cells were investigated, which provide support to analyze the effect of TSA on cancer cells. Results： Cell cycle arrest effect in G0/G1 of the lower density cells was more obvious than that in other groups. The other discovery in this work was that the cellular UV absorption value was higher when the density of cultured cell was lower. Conclusion： This experiment would guide the clinical study on early or late stage cancer patients in the future. On the other hand, this work indicates when cells were arrested in G0/G1 phase, the cellular absorption value increased at the same time, so UV absorption spectrum could characterize the change of cell cycle.
文摘Geometry optimization of p-C_(6)H_(4)-connected cyclo[20]carbon(p-C_(6)H_(4)-C_(20))was carried out at M062X/6-311G(d,p)level,three kinds of bond orders(Mayer,Laplacian,and Wiberg),electron-hole distributions,localized orbital locators(LOL),and infrared(IR)spectrum were also performed at the same level.Based on TD-DFT M062X/6-311G(d,p)method,the first 20 excited states and ultraviolet(UV)spectra of p-C_(6)H_(4)-C_(20) were calculated.Calculation results of π-electron delocalization analyses prove thatπ-electron delocalization of p-C_(6)H_(4)-C_(20) is more likely to occur on shorter C-C bonds rather than longer C-C bonds,and inside/outside of the ring plane rather than above/below the ring plane.Two absorption peaks of p-C_(6)H_(4)-C_(20) locate at about 319 nm and 236 nm,respectively.
