The purpose of the present study was to compare the effects of different doses of ultraviolet radiation A1 （UVA1） on human fibroblast proliferation and collagen level in a mouse model of scleroderma, so as to identi...The purpose of the present study was to compare the effects of different doses of ultraviolet radiation A1 （UVA1） on human fibroblast proliferation and collagen level in a mouse model of scleroderma, so as to identify appropriate irradiation doses for clinical treatment of scleroderma. Monolayer from human fibroblasts was cultured in vitro, and a mouse model of scleroderma was established by subcutaneous injection of 100 μL of 400 μg/mL bleomycin into the back of BALB/c mice for 4 weeks. The mouse models and human fibroblasts were divided into UVA1- exposed （100, 60 and 20 J/cm2） and UVA-unexposed groups. At 0, 24 and 48 h after exposure, cell proliferation and levels of hydroxyproline and collagen were detected. UVA1 irradiation was performed 3 times weekly for 10 weeks, and the pathological changes of skin tissues, skin thickness and collagen level were observed after phototherapy. Cell proliferation and the levels of hydroxyproline and collagen were inhibited after phototherapy, and there was a significant difference between the UVAl-exposed cells and UVAl-unexposed cells （P 〈 0.001）. In addition, UVA1 phototherapy improved dermal sclerosis and softened the skin, and there were significant differences between the high-dose UVA1 group and the model group, and the negative group （P 〈 0.05）. It is concluded that UVA1 radiation can reduce cell proliferation, and decrease hydroxyproline and collagen levels in a dose-dependent manner in vitro. High-dose UVA1 phototherapy has marked therapeutic effect on scleroderma in the mouse model. Decreased collagen level may be related to the reduced number and activity of cells, as well as inhibition of collagen synthesis.展开更多
Ultraviolet blood irradiation has been used as a physical therapy to treat many nonspeci¯c diseases in clinics;however,the underlying mechanisms remain largely unclear.Neutrophils,the first line of host defense,p...Ultraviolet blood irradiation has been used as a physical therapy to treat many nonspeci¯c diseases in clinics;however,the underlying mechanisms remain largely unclear.Neutrophils,the first line of host defense,play a crucial role in a variety of in°ammatory responses.In the present work,we investigated the effects of ultraviolet light A(UVA)on the immune functions of human neutrophils at the single-cell level by using an inverted°uorescence microscope.N-Formylmethionyl-leucyl-phenylalanine(FMLP),a classic physiological chemotactic peptide,was used to induce a series of immune responses in neutrophils in vitro.FMLP-induced calcium mobilization,migration,and phagocytosis in human neutrophils was significantly blocked after treatment with 365 nm UVA irradiation,demonstrating the immunosuppressive effects of UVA irradiation on neutrophils.Similar responses were also observed when the cells were pretreated with H2O2,a type of reactive oxygen species(ROS).Furthermore,UVA irradiation resulted in an increase in NAD(P)H,a member of host oxidative stress in cells.Taken together,our data indicate that UVA irradiation results in immunosuppression associated with the production of ROS in human neutrophils.展开更多
During normal metabolism,oxidative bypro-ducts will inevitably generate and damage molecules thereby impairing their biological functions,including the aging process.Bushenkangshuai Tang(补肾抗衰方,BT)is a traditional...During normal metabolism,oxidative bypro-ducts will inevitably generate and damage molecules thereby impairing their biological functions,including the aging process.Bushenkangshuai Tang(补肾抗衰方,BT)is a traditional Chinese medicine widely used for clini-cally treating premature ovarian failure.In the present study,BT administration at high concentrations signifi-cantly increased lifespan,slowed aging-related decline,and delayed accumulation of aging-related cellular damage in wild-type Caenorhabditis elegans.BT admin-istration could further largely alleviate the aging defects induced by UV and oxidative stresses,and BT administra-tion at different concentrations could largely rescue the aging defects in mev-1 mutant animals.The protective effects of BT administration on aging process were at least partially dependent on the Ins/IGF-like signaling pathway.Moreover,BT administration at different concentrations obviously altered the expression patterns of antioxidant genes and suppressed the severe stress responses induced by UV and oxidative stresses,suggesting that BT-induced tolerance to UV or oxidative stress might result from reactive oxygen species scavenging.BT administration during development was not necessarily a requirement for UV and oxidative stress resistance,and the concentrations of administrated BT examined were not toxic for nematodes.Therefore,BT administration could effectively retrieve the aging defects induced by UV irradiation and oxidative stress in Caenorhabditis elegans.展开更多
文摘The purpose of the present study was to compare the effects of different doses of ultraviolet radiation A1 （UVA1） on human fibroblast proliferation and collagen level in a mouse model of scleroderma, so as to identify appropriate irradiation doses for clinical treatment of scleroderma. Monolayer from human fibroblasts was cultured in vitro, and a mouse model of scleroderma was established by subcutaneous injection of 100 μL of 400 μg/mL bleomycin into the back of BALB/c mice for 4 weeks. The mouse models and human fibroblasts were divided into UVA1- exposed （100, 60 and 20 J/cm2） and UVA-unexposed groups. At 0, 24 and 48 h after exposure, cell proliferation and levels of hydroxyproline and collagen were detected. UVA1 irradiation was performed 3 times weekly for 10 weeks, and the pathological changes of skin tissues, skin thickness and collagen level were observed after phototherapy. Cell proliferation and the levels of hydroxyproline and collagen were inhibited after phototherapy, and there was a significant difference between the UVAl-exposed cells and UVAl-unexposed cells （P 〈 0.001）. In addition, UVA1 phototherapy improved dermal sclerosis and softened the skin, and there were significant differences between the high-dose UVA1 group and the model group, and the negative group （P 〈 0.05）. It is concluded that UVA1 radiation can reduce cell proliferation, and decrease hydroxyproline and collagen levels in a dose-dependent manner in vitro. High-dose UVA1 phototherapy has marked therapeutic effect on scleroderma in the mouse model. Decreased collagen level may be related to the reduced number and activity of cells, as well as inhibition of collagen synthesis.
基金This work was supported by the National Natural Science Foundation of China(No.11204142),the National Basic Research Program of China (No.2013CB328702)International S&T Cooperation Program of China (2011DFA52870)+1 种基金the 111 Project(No.B07013),the National Science Fund for Talent Training in Basic Sciences(No.J1103208),the PCSIRT (IRT0149)the Social Development Fund of Ge'ermu Science and Technology Bureau and the State Key Laboratory of Medicinal Chemical Biology.
文摘Ultraviolet blood irradiation has been used as a physical therapy to treat many nonspeci¯c diseases in clinics;however,the underlying mechanisms remain largely unclear.Neutrophils,the first line of host defense,play a crucial role in a variety of in°ammatory responses.In the present work,we investigated the effects of ultraviolet light A(UVA)on the immune functions of human neutrophils at the single-cell level by using an inverted°uorescence microscope.N-Formylmethionyl-leucyl-phenylalanine(FMLP),a classic physiological chemotactic peptide,was used to induce a series of immune responses in neutrophils in vitro.FMLP-induced calcium mobilization,migration,and phagocytosis in human neutrophils was significantly blocked after treatment with 365 nm UVA irradiation,demonstrating the immunosuppressive effects of UVA irradiation on neutrophils.Similar responses were also observed when the cells were pretreated with H2O2,a type of reactive oxygen species(ROS).Furthermore,UVA irradiation resulted in an increase in NAD(P)H,a member of host oxidative stress in cells.Taken together,our data indicate that UVA irradiation results in immunosuppression associated with the production of ROS in human neutrophils.
基金supported by the grants from the National Natural Science Foundation of China(Grant No.30771113)the Program for New Century Excellent Talents in University,and the Nanjing Scientific and Technical Plan(No.200701113).
文摘During normal metabolism,oxidative bypro-ducts will inevitably generate and damage molecules thereby impairing their biological functions,including the aging process.Bushenkangshuai Tang(补肾抗衰方,BT)is a traditional Chinese medicine widely used for clini-cally treating premature ovarian failure.In the present study,BT administration at high concentrations signifi-cantly increased lifespan,slowed aging-related decline,and delayed accumulation of aging-related cellular damage in wild-type Caenorhabditis elegans.BT admin-istration could further largely alleviate the aging defects induced by UV and oxidative stresses,and BT administra-tion at different concentrations could largely rescue the aging defects in mev-1 mutant animals.The protective effects of BT administration on aging process were at least partially dependent on the Ins/IGF-like signaling pathway.Moreover,BT administration at different concentrations obviously altered the expression patterns of antioxidant genes and suppressed the severe stress responses induced by UV and oxidative stresses,suggesting that BT-induced tolerance to UV or oxidative stress might result from reactive oxygen species scavenging.BT administration during development was not necessarily a requirement for UV and oxidative stress resistance,and the concentrations of administrated BT examined were not toxic for nematodes.Therefore,BT administration could effectively retrieve the aging defects induced by UV irradiation and oxidative stress in Caenorhabditis elegans.
