The ultracytochemical effects in the liver of rabbit undergoing high energy shock wave (HESW) were studied with electron microscope. The application of lanthanum as a tracer for ultrastructural study demonstrated that...The ultracytochemical effects in the liver of rabbit undergoing high energy shock wave (HESW) were studied with electron microscope. The application of lanthanum as a tracer for ultrastructural study demonstrated that intracellular lanthanum could be observed, most of which entered the hepatocytes and its mitochondria. The lanthanum granules were also found to deposite in the zone of tight junctions of bile canaliculi, which indicated that the tight junctions had been damaged. The activities of succinate dehydrogenase (SDH) in liver cells, alkaline phosphatase (ALP) and thiamine pyrophosphatase (TPPase) on the wall of bile canaliculi became diminished obviously. Both the activites and localizations of TPPase had changed. Some TPPase from the damaged lysosome like vesicles and Golgi saccules of liver cells discharged into cytoplasm. TPPase reaction production in some bile canaliculi decreased. In the intercellular space of the liver cells and the tight junctions of bile canaliculi TPPase reaction could be seen. Ultrastructurally, the changes commonly seen were hydropic mitochondria and dilatation of rough endoplasmic reticulum. Serologic test demonstrated that there was an abnormal change of SGPT, SGOT and ALP. The results showed that HESW can damage the ultrastructure and function of liver.展开更多
Objective: To explore the superoxide(O-2,) - producing site and the O -2-releasing channel in the rat blood neutrophils. Methods: After rat neutrophils were isolated from the whole blood, the O-2 production with or wi...Objective: To explore the superoxide(O-2,) - producing site and the O -2-releasing channel in the rat blood neutrophils. Methods: After rat neutrophils were isolated from the whole blood, the O-2 production with or without phorbol 12-myristate 13-acetate(PMA,an activator of protein kinase C)-stimulation was observed ultrastructurally. Canonized ferritin (CF) functions was used as a marker for endocytosis while the alkaline phosphatase (ALPase) as a marker enzyme for a certain type of intracellular neutrophil granules. The in situ intracellular localization of the oxidation product site was determined using the DAB/Mn method, the CF-marker method, and also the co-localization of O -2with alkaline phosphatase (ALPase) activity. Results: In unstimulated neutrophils, the production of O-2 was hardly detected and the CF was distributed only over the surface of the cell membrane. Upon stimulation by PMA, however, the reaction product of O -2 was located in some granules of the neutrophils, and then fused directly with the plasma membrane or fused to form larger intracellular vesicles. Using CF as a tracer of the endocytosis of the plasma membrane upon cell stimulation, we found that these O-2-positive granules were originally devoid of CF particles. With the prolongation of the PMA-stimulation, the number of vesicles containing oxidation reaction product decreased, but the intracellular granules containing both the oxidation product and CF particles increased. ALPase activity was also localized in the oxidation-positive intracellular granules, and the time course of up-regulation of ALPase activity to the cell surface was paralleled with the release of O -2 from the stimulated neutrophils. Conclusion: These findings implied (1) O -2 is released from the stimulated rat neutrophils through exocytosis; (2) the O-2 production was initiated in the cytoplasmic ALPase-positive granules but not on the plasma membrane of the rat neutrophils.展开更多
文摘The ultracytochemical effects in the liver of rabbit undergoing high energy shock wave (HESW) were studied with electron microscope. The application of lanthanum as a tracer for ultrastructural study demonstrated that intracellular lanthanum could be observed, most of which entered the hepatocytes and its mitochondria. The lanthanum granules were also found to deposite in the zone of tight junctions of bile canaliculi, which indicated that the tight junctions had been damaged. The activities of succinate dehydrogenase (SDH) in liver cells, alkaline phosphatase (ALP) and thiamine pyrophosphatase (TPPase) on the wall of bile canaliculi became diminished obviously. Both the activites and localizations of TPPase had changed. Some TPPase from the damaged lysosome like vesicles and Golgi saccules of liver cells discharged into cytoplasm. TPPase reaction production in some bile canaliculi decreased. In the intercellular space of the liver cells and the tight junctions of bile canaliculi TPPase reaction could be seen. Ultrastructurally, the changes commonly seen were hydropic mitochondria and dilatation of rough endoplasmic reticulum. Serologic test demonstrated that there was an abnormal change of SGPT, SGOT and ALP. The results showed that HESW can damage the ultrastructure and function of liver.
文摘Objective: To explore the superoxide(O-2,) - producing site and the O -2-releasing channel in the rat blood neutrophils. Methods: After rat neutrophils were isolated from the whole blood, the O-2 production with or without phorbol 12-myristate 13-acetate(PMA,an activator of protein kinase C)-stimulation was observed ultrastructurally. Canonized ferritin (CF) functions was used as a marker for endocytosis while the alkaline phosphatase (ALPase) as a marker enzyme for a certain type of intracellular neutrophil granules. The in situ intracellular localization of the oxidation product site was determined using the DAB/Mn method, the CF-marker method, and also the co-localization of O -2with alkaline phosphatase (ALPase) activity. Results: In unstimulated neutrophils, the production of O-2 was hardly detected and the CF was distributed only over the surface of the cell membrane. Upon stimulation by PMA, however, the reaction product of O -2 was located in some granules of the neutrophils, and then fused directly with the plasma membrane or fused to form larger intracellular vesicles. Using CF as a tracer of the endocytosis of the plasma membrane upon cell stimulation, we found that these O-2-positive granules were originally devoid of CF particles. With the prolongation of the PMA-stimulation, the number of vesicles containing oxidation reaction product decreased, but the intracellular granules containing both the oxidation product and CF particles increased. ALPase activity was also localized in the oxidation-positive intracellular granules, and the time course of up-regulation of ALPase activity to the cell surface was paralleled with the release of O -2 from the stimulated neutrophils. Conclusion: These findings implied (1) O -2 is released from the stimulated rat neutrophils through exocytosis; (2) the O-2 production was initiated in the cytoplasmic ALPase-positive granules but not on the plasma membrane of the rat neutrophils.
