The fingerling Grass carp. Ctenopharyngodon idellus. was exposed in differentconcentrations of meothrin nearby 24h LC_(50) and its gill morphology was examined by light and elec-tron microscopy. The results indicate...The fingerling Grass carp. Ctenopharyngodon idellus. was exposed in differentconcentrations of meothrin nearby 24h LC_(50) and its gill morphology was examined by light and elec-tron microscopy. The results indicate that meothrin caused fish convulsions. cough, ataxia,intermittent paralysis and overtumed. Morphological examination revealved severe alterations to com-pare with control fish. These initially consisted in hypertrophy of the secondary lamellae, andfinally resulted in local telangiectasia, fusion between secondary lamellae.Ultrustiuctural changes in the gills showed the pillar cell system collapse and resulted in largenon-tissue spaces which were invaded by leucocytes. The cytoplasin of the epithelial cells appearedmany myelin figures, electron dense deposits. Chloride cells were severely damaged with a degeneratedmitochondria and nuclear membrane. Higher concentration of meothrin caused a loss of adhesion be-tween the epithelial cells. accompanied by a collapsing of the structural integrity of the primarylamellae and degenerating of epithelial and chloride cells .展开更多
The myelin sheath is a lipoprotein-rich,multilayered structure capable of increasing conduction velocity in central and peripheral myelinated nerve fibers.Due to the complex structure and composition of myelin,various...The myelin sheath is a lipoprotein-rich,multilayered structure capable of increasing conduction velocity in central and peripheral myelinated nerve fibers.Due to the complex structure and composition of myelin,various histological techniques have been developed over the centuries to evaluate myelin under normal,pathological or experimental conditions.Today,methods to assess myelin integrity or content are key tools in both clinical diagnosis and neuroscience research.In this review,we provide an updated summary of the composition and structure of the myelin sheath and discuss some histological procedures,from tissue fixation and processing techniques to the most used and practical myelin histological staining methods.Considering the lipoprotein nature of myelin,the main features and technical details of the different available methods that can be used to evaluate the lipid or protein components of myelin are described,as well as the precise ultrastructural techniques.展开更多
文摘The fingerling Grass carp. Ctenopharyngodon idellus. was exposed in differentconcentrations of meothrin nearby 24h LC_(50) and its gill morphology was examined by light and elec-tron microscopy. The results indicate that meothrin caused fish convulsions. cough, ataxia,intermittent paralysis and overtumed. Morphological examination revealved severe alterations to com-pare with control fish. These initially consisted in hypertrophy of the secondary lamellae, andfinally resulted in local telangiectasia, fusion between secondary lamellae.Ultrustiuctural changes in the gills showed the pillar cell system collapse and resulted in largenon-tissue spaces which were invaded by leucocytes. The cytoplasin of the epithelial cells appearedmany myelin figures, electron dense deposits. Chloride cells were severely damaged with a degeneratedmitochondria and nuclear membrane. Higher concentration of meothrin caused a loss of adhesion be-tween the epithelial cells. accompanied by a collapsing of the structural integrity of the primarylamellae and degenerating of epithelial and chloride cells .
基金supported by the Spanish“Plan Nacional de Investigación Científica,Desarrollo e Innovación Tecnológica,Ministerio de Economía y Competitividad(Instituto de Salud CarlosⅢ)”,Grant FIS PI20-0318 co-financed by“Fondo Europeo de Desarrollo Regional ERDF-FEDER European Union”Grant P18-RT-5059“Plan Andaluz de Investigación,Desarrollo e Innovación(PAIDI 2020),Consejería de Transformación Económica,Industria,Conocimiento y Universidades,Junta de Andalucía,Espana”(all to VC)Grant PPJIA202219“Ayudas del plan propio UGR 2022,Plan propio de investigación y transferencia,Universidad de Granada,Espana”(to JCA andóDGG)。
文摘The myelin sheath is a lipoprotein-rich,multilayered structure capable of increasing conduction velocity in central and peripheral myelinated nerve fibers.Due to the complex structure and composition of myelin,various histological techniques have been developed over the centuries to evaluate myelin under normal,pathological or experimental conditions.Today,methods to assess myelin integrity or content are key tools in both clinical diagnosis and neuroscience research.In this review,we provide an updated summary of the composition and structure of the myelin sheath and discuss some histological procedures,from tissue fixation and processing techniques to the most used and practical myelin histological staining methods.Considering the lipoprotein nature of myelin,the main features and technical details of the different available methods that can be used to evaluate the lipid or protein components of myelin are described,as well as the precise ultrastructural techniques.
