AIM:To explore the DNA methylation of COL4A1 in ultraviolet-B(UVB)-induced age-related cataract(ARC)models in vitro and in vivo.METHODS:Human lens epithelium B3(HLEB3)cells and Sprague Dawley rats were exposure to UVB...AIM:To explore the DNA methylation of COL4A1 in ultraviolet-B(UVB)-induced age-related cataract(ARC)models in vitro and in vivo.METHODS:Human lens epithelium B3(HLEB3)cells and Sprague Dawley rats were exposure to UVB respectively.The MTT assay was utilized to evaluate cell proliferation.Flow cytometry was employed for analysis of cell apoptosis and cell cycle.COL4A1 expression in HLEB3 cells and anterior lens capsules were assessed using Western blot and reverse transcription-polymerase chain reaction(RTPCR).The localization of COL4A1 in HLEB3 cells was determined by immunofluorescence.The methylation status of CpG islands located in COL4A1 promoter was verified using bisulfite-sequencing PCR(BSP).DNMTs and TETs mRNA levels was examined by RT-PCR.RESULTS:UVB exposure decreased HLEB3 cells proliferation,while increased the apoptosis rate and cells were arrested in G0/G1 phase.COL4A1 expression was markedly inhibited in UVB treated cells compared to the controls.Hypermethylation status was detected in the CpG islands within COL4A1 promoter in HLEB3 cells subjected to UVB exposure.Expressions of DNMTs including DNMT1/2/3 were elevated in UVB treated HLEB3 cells compared to that in the controls,while expressions of TETs including TET1/2/3 showed the opposite trend.Results from the UVB treated rat model further confirmed the decreased expression of COL4A1,hypermethylation status of the CpG islands at promoter of COL4A1 and abnormal expression of DNMT1/2/3 and TET1/2/in UVB exposure group.CONCLUSION:DNA hypermethylation of COL4A1 promoter CpG islands is correlated with decreased COL4A1 expression in UVB induced HLEB3 cells and anterior lens capsules of rats.展开更多
[Objective] The study aimed at investigating the influence of enhanced UV-B radiation on photosynthesis of grapevine.[Method] The seedlings of Cabernet sauvignon were treated with different intensities of UV-B radiati...[Objective] The study aimed at investigating the influence of enhanced UV-B radiation on photosynthesis of grapevine.[Method] The seedlings of Cabernet sauvignon were treated with different intensities of UV-B radiation under outdoor conditions,and then the contents of photosynthetic pigments and flavonoids in leaves were determined by measuring the absorbance of leaves extracts at 663,645,470 and 300 nm,respectively.[Result] The content of photosynthetic pigments in the leaves of grapevine obviously increased with time under the treatments of different enhanced UV-B radiation.Compared with the control,the chlorophyll a,chlorophyll b,total chlorophyll and carotenoid were obviously increased by 5%,2%,4% and 3% in the enhanced UV-B radiation treatment of 10.8 μW/cm2（T1）,and in the treatment of 25.6 μW/cm2（T2） the corresponding levels were subsequently increased by 11%,9%,10% and 7% with a significant increase in the content of chlorophyll a.On the other hand,the flavonoids content in the leaves of grapevine were obviously increased by 13%,9% in T1 and T2.[Conclusion] The grapevine has strong adaptability to UV-B radiation,and appropriate enhanced UV-B radiation couldn＇t decrease the photosynthesis of grapevine leaves.展开更多
[Objective] Effects of different concentrations of nitric oxide on membrane lipid peroxidation of wheat induced by enhanced UV-B radiation were researched,sodium nitroprusside （SNP） was selected as an exogenous nitr...[Objective] Effects of different concentrations of nitric oxide on membrane lipid peroxidation of wheat induced by enhanced UV-B radiation were researched,sodium nitroprusside （SNP） was selected as an exogenous nitric oxide（NO）donor.[Method] There are 3 groups including CK,UV treatment group （B）,B＋SNP treatment group,0,1,2,3,4 d sampling after treatment respectively,and physiological and biochemical indexes of MDA content and CAT,POD,SOD and so on were determined,repeated 3 times,and statistical analyzed.[Result] The results showed that,after the enhanced UV-B radiation,activity of the catalase （CAT）,superoxide dismutase （SOD） and of the guaiacol peroxidase （POD） all reduced apparently,and the concentration of malondialdehyde （MDA） increased obviously,leading to oxidative damage in wheat seedlings.Impose different concentrations of SNP after UV-B radiation,may mitigate oxidative damage of wheat seedling from different degrees,which was in agreement with the effect of making the concentration of MDA decrease and the activity of the CAT,SOD and POD all increased.The mitigation role of 0.01 mol/L SNP was more obvious for roots＇ oxidative damage,while 0.1 mmol/L SNP is more effective for oxidative damage of leaves.[Conclusion] Exogenous NO donor SNP had obvious relieve effects on oxidative damage of wheat seedlings caused by UV-B radiation,which can enhance adaptive capacity of plants to adversity stress.展开更多
Effect of cerium （Ce^3＋） on growth and photosynthesis in rape seedlings exposed to two levels of ultraviolet-B radiation （UV-B, 280 - 320 nm） was studied with hydroponics under laboratory conditions. The growth o...Effect of cerium （Ce^3＋） on growth and photosynthesis in rape seedlings exposed to two levels of ultraviolet-B radiation （UV-B, 280 - 320 nm） was studied with hydroponics under laboratory conditions. The growth of rape seedlings exposed to two levels of UV-B irradiation （0.15 and 0.35 W· m^-2/T2） was both heavily restrained. The aboveground growth indices including stem （plant） height, leaf number, leaf area, leaf fresh/dry weight and stem fresh/dry weight were obviously decreased by 13.2% - 44.1% （T1） and 21 .4% - 49.3% （T2）. Compared to CK, and except active absorption area of roots, the belowground indices main root length, root volume and fresh/dry weight by 14.1% -35.6% （T1） and 20.3% - 42.6% （T2）, respectively. For Ce ＋ UV-B treatments, the aboveground and belowground growth indices were decreased by 4.1% - 23.6%, 5.2% -23.3% （Ce＋T1） and 10.8% -28.4%, 7.0% -27.8% （Ce ＋T2）, lower than those of UV-B treatments mentioned above. These results show that Ce has protective effect on plant against injury of UV-B radiation. Furthermore, the protective effect of Ce on seedlings exposed to T1 level of UV-B radiation is superior to T2 level. Chlorophyll content, net photosynthesis rate, transpiration rate, stomatal conductance and water use efficiency in UV-B treatments decrease dramatically, whereas intercellular CO2 concentration increases. Although these indices in Ce ＋ UV-B treatments decrease compared with those of CK, the decrease in Ce ＋ UV-B treatments are lower than those in UV-B treatment. This phenomenon indicates that the ecophysiological protective effect of Ce is based on improving photosynthesis in plants. The dynamic curves of photosynthesis indices show that the course of light-repair is shortened and the injury to rape seedlings by UV-B radiation stress is alleviated by Ce.展开更多
In order to investigate the effects of lanthanum（Ⅲ） on cell ultrastructure of soybean leaves under elevated ultraviolet-B irradiation （UV-B, 280-320 nm）, the chloroplast ultrastructure of soybean seedlings was st...In order to investigate the effects of lanthanum（Ⅲ） on cell ultrastructure of soybean leaves under elevated ultraviolet-B irradiation （UV-B, 280-320 nm）, the chloroplast ultrastructure of soybean seedlings was studied by hydroponics under laboratory conditions. The resuits showed that the thylakoid in chloroplast was orderly and clearly as soybean leaves were pretreated by La（Ⅲ）. The thylakoid was indis- tinctly disordered, expanded and even indiscoverable in the chloroplast under UV-B stress. The impact on the thylakoid by the high intensity UV-B irradiation （T2） was bigger than that by the low intensity UV-B irradiation （T1）. However, the destruction of the chloroplast structure caused by UV-B stress was alleviated by La（Ⅲ）, and the arrangement of the thylakoid in the chloroplast became orderly and clearly. The effect of the alleviation by La（Ⅲ） under the low intensity UV-B irradiation （T1） was better than that under the high intensity UV-B irradiation （T2）.展开更多
The effect of La on flavonoids, chlorophyll contents, and phenylalanine ammonia-lyase （PAL） activity in soybean seedlings under supplementary ultraviolet-B radiation （UV-B, 280 - 320 nm） was studied. The results s...The effect of La on flavonoids, chlorophyll contents, and phenylalanine ammonia-lyase （PAL） activity in soybean seedlings under supplementary ultraviolet-B radiation （UV-B, 280 - 320 nm） was studied. The results show that PAL activity, contents of flavonoids and chlorophyll in the plants pretreated with La （20 mg·L^- 1 ） are higher than those in CK. UV-B radiation could result in an increase in flavonoid content and PAL activity, associated with a decrease in chlorophyll content. However, the increase in the range of PAL activity and flavonoid content in UV-B treatment are lesser than those in the La treatment. The changes of flavonoid contents and PAL activity in La ＋ UV-B treatment are similar to those in UV-B treatment, and the increase in their range is higher than those in UV-B treatment. This shows that La can enhance the resistance of soybean seedling to UV-B radiation and alleviate the damage of UV-B radiation by increasing flavonoid content, chlorophyll content, and PAL activity.展开更多
Wheat (Triticum aestivum), as a kind of important economic crop cultured in the Northern China, is affected by present-day enhanced ultraviolet-B (UV-B) radiation. To get the information of the impact by UV-B radiatio...Wheat (Triticum aestivum), as a kind of important economic crop cultured in the Northern China, is affected by present-day enhanced ultraviolet-B (UV-B) radiation. To get the information of the impact by UV-B radiation on it, the proteins of wheat (Jin mai NO.8) leaves, which were divided into the normal light group (CK) and UV-B radiation group (B), were extracted and ran at SDS-PAGE at different treatment days (5, 6, 7). The proteins were also analyzed by run two-dimensional gel electrophoresis (2-DE), which allowed the identification of some significantly different gel spots. The proteins spots were further verified by Matrix-Assisted Laser Desorption/lonization-time of Flight Mass Spectrometry. The results showed: 1) the enhanced UV-B affects the growth of the wheat, as the visual changes appear on the sixth day;2) the proteins expressions between the B group and the CK group were remarkably different on the sixth day;3) the proteins of wheat leaves of the sixth day were further analyzed by 2-DE revealed that twenty-one protein points were identificated between the B group and the CK group. Among these twenty-one proteins, six proteins of them were up-regulated and twelve proteins of them were down-regulated, three new proteins were expressed only in the B group. Three proteins among six proteins, which were up-regulated, were further verified as RuBisCo large subunit binding protein;SOD;Calmodulin. The result indicates wheat could improve genes encoding proteins in their leaves and protect themselves, when enhanced UV-B affects the growth of the wheat.展开更多
目的:探究窄谱中波紫外线联合0.1%他克莫司软膏治疗白癜风患者疗效及对皮损面积和色素积分的影响。方法:将笔者医院皮肤科于2020年1月-2023年1月收治的96例门诊患者纳为研究对象,根据随机数字法将所有患者分为对照组与观察组,每组各48例...目的:探究窄谱中波紫外线联合0.1%他克莫司软膏治疗白癜风患者疗效及对皮损面积和色素积分的影响。方法:将笔者医院皮肤科于2020年1月-2023年1月收治的96例门诊患者纳为研究对象,根据随机数字法将所有患者分为对照组与观察组,每组各48例,给予对照组患者外涂0.1%他克莫司软膏治疗;观察组在对照组治疗基础上联合窄谱中波紫外线治疗。对比两组患者整体疗效,治疗前后皮损面积(Wood灯下皮损评估)与色素积分、不同部位严重性指数(Psoriasis area and severity index,PASI)与白癜风面积指数(Vitiligo area and severity index,VASI),不良反应发生情况及复发率。结果:观察组总有效率(97.92%)显著高于对照组(85.42%)(P<0.05);治疗后,两组患者皮损面积显著降低(P<0.05),且观察组显著低于对照组(P<0.05);治疗后,两组色素积分显著上升(P<0.05),且观察组显著高于对照组(P<0.05);治疗后,观察组各部位(躯干、四肢、面部)PASI与VASI均显著低于对照组(P<0.05);两组患者治疗期间不良反应发生率[观察组(16.67%) vs.对照组(10.42%)]与复发率[观察组(6.25%)vs.对照组(8.33%)]差异无统计学意义(P>0.05)。结论:窄谱中波紫外线联合0.1%他克莫司软膏可显著改善白癜风患者病情,缓解皮肤损伤,增加皮肤色素沉着,安全有效,值得临床推广。展开更多
基金Supported by the Shaanxi Provincial Department of Science and Technology Agency Project(No.2022SF-502)Xi’an Medical University Scientific Research Capacity Improvement Project(No.2022NLTS104)+2 种基金The Fifth Batch of Key Disciplines of Xi’an Medical University(No.medical technology12202306)Yunzhen Optometry Special Fund(No.2021HXZR10)Innovation and Entrepreneurship Training Program for College Students(No.S202211840043).
文摘AIM:To explore the DNA methylation of COL4A1 in ultraviolet-B(UVB)-induced age-related cataract(ARC)models in vitro and in vivo.METHODS:Human lens epithelium B3(HLEB3)cells and Sprague Dawley rats were exposure to UVB respectively.The MTT assay was utilized to evaluate cell proliferation.Flow cytometry was employed for analysis of cell apoptosis and cell cycle.COL4A1 expression in HLEB3 cells and anterior lens capsules were assessed using Western blot and reverse transcription-polymerase chain reaction(RTPCR).The localization of COL4A1 in HLEB3 cells was determined by immunofluorescence.The methylation status of CpG islands located in COL4A1 promoter was verified using bisulfite-sequencing PCR(BSP).DNMTs and TETs mRNA levels was examined by RT-PCR.RESULTS:UVB exposure decreased HLEB3 cells proliferation,while increased the apoptosis rate and cells were arrested in G0/G1 phase.COL4A1 expression was markedly inhibited in UVB treated cells compared to the controls.Hypermethylation status was detected in the CpG islands within COL4A1 promoter in HLEB3 cells subjected to UVB exposure.Expressions of DNMTs including DNMT1/2/3 were elevated in UVB treated HLEB3 cells compared to that in the controls,while expressions of TETs including TET1/2/3 showed the opposite trend.Results from the UVB treated rat model further confirmed the decreased expression of COL4A1,hypermethylation status of the CpG islands at promoter of COL4A1 and abnormal expression of DNMT1/2/3 and TET1/2/in UVB exposure group.CONCLUSION:DNA hypermethylation of COL4A1 promoter CpG islands is correlated with decreased COL4A1 expression in UVB induced HLEB3 cells and anterior lens capsules of rats.
文摘[Objective] The study aimed at investigating the influence of enhanced UV-B radiation on photosynthesis of grapevine.[Method] The seedlings of Cabernet sauvignon were treated with different intensities of UV-B radiation under outdoor conditions,and then the contents of photosynthetic pigments and flavonoids in leaves were determined by measuring the absorbance of leaves extracts at 663,645,470 and 300 nm,respectively.[Result] The content of photosynthetic pigments in the leaves of grapevine obviously increased with time under the treatments of different enhanced UV-B radiation.Compared with the control,the chlorophyll a,chlorophyll b,total chlorophyll and carotenoid were obviously increased by 5%,2%,4% and 3% in the enhanced UV-B radiation treatment of 10.8 μW/cm2（T1）,and in the treatment of 25.6 μW/cm2（T2） the corresponding levels were subsequently increased by 11%,9%,10% and 7% with a significant increase in the content of chlorophyll a.On the other hand,the flavonoids content in the leaves of grapevine were obviously increased by 13%,9% in T1 and T2.[Conclusion] The grapevine has strong adaptability to UV-B radiation,and appropriate enhanced UV-B radiation couldn＇t decrease the photosynthesis of grapevine leaves.
基金Supported by National Natural Science Foundation of China(No.30671061)Natural Science Foundation of Shanxi Province(No.2008011059-1 and No.20041101)~~
文摘[Objective] Effects of different concentrations of nitric oxide on membrane lipid peroxidation of wheat induced by enhanced UV-B radiation were researched,sodium nitroprusside （SNP） was selected as an exogenous nitric oxide（NO）donor.[Method] There are 3 groups including CK,UV treatment group （B）,B＋SNP treatment group,0,1,2,3,4 d sampling after treatment respectively,and physiological and biochemical indexes of MDA content and CAT,POD,SOD and so on were determined,repeated 3 times,and statistical analyzed.[Result] The results showed that,after the enhanced UV-B radiation,activity of the catalase （CAT）,superoxide dismutase （SOD） and of the guaiacol peroxidase （POD） all reduced apparently,and the concentration of malondialdehyde （MDA） increased obviously,leading to oxidative damage in wheat seedlings.Impose different concentrations of SNP after UV-B radiation,may mitigate oxidative damage of wheat seedling from different degrees,which was in agreement with the effect of making the concentration of MDA decrease and the activity of the CAT,SOD and POD all increased.The mitigation role of 0.01 mol/L SNP was more obvious for roots＇ oxidative damage,while 0.1 mmol/L SNP is more effective for oxidative damage of leaves.[Conclusion] Exogenous NO donor SNP had obvious relieve effects on oxidative damage of wheat seedlings caused by UV-B radiation,which can enhance adaptive capacity of plants to adversity stress.
文摘Effect of cerium （Ce^3＋） on growth and photosynthesis in rape seedlings exposed to two levels of ultraviolet-B radiation （UV-B, 280 - 320 nm） was studied with hydroponics under laboratory conditions. The growth of rape seedlings exposed to two levels of UV-B irradiation （0.15 and 0.35 W· m^-2/T2） was both heavily restrained. The aboveground growth indices including stem （plant） height, leaf number, leaf area, leaf fresh/dry weight and stem fresh/dry weight were obviously decreased by 13.2% - 44.1% （T1） and 21 .4% - 49.3% （T2）. Compared to CK, and except active absorption area of roots, the belowground indices main root length, root volume and fresh/dry weight by 14.1% -35.6% （T1） and 20.3% - 42.6% （T2）, respectively. For Ce ＋ UV-B treatments, the aboveground and belowground growth indices were decreased by 4.1% - 23.6%, 5.2% -23.3% （Ce＋T1） and 10.8% -28.4%, 7.0% -27.8% （Ce ＋T2）, lower than those of UV-B treatments mentioned above. These results show that Ce has protective effect on plant against injury of UV-B radiation. Furthermore, the protective effect of Ce on seedlings exposed to T1 level of UV-B radiation is superior to T2 level. Chlorophyll content, net photosynthesis rate, transpiration rate, stomatal conductance and water use efficiency in UV-B treatments decrease dramatically, whereas intercellular CO2 concentration increases. Although these indices in Ce ＋ UV-B treatments decrease compared with those of CK, the decrease in Ce ＋ UV-B treatments are lower than those in UV-B treatment. This phenomenon indicates that the ecophysiological protective effect of Ce is based on improving photosynthesis in plants. The dynamic curves of photosynthesis indices show that the course of light-repair is shortened and the injury to rape seedlings by UV-B radiation stress is alleviated by Ce.
基金supported by the National Natural Science Foundation of China (30570323)the Special Foundation for Rare Earth of State Planning Committee (IFZ20051210)
文摘In order to investigate the effects of lanthanum（Ⅲ） on cell ultrastructure of soybean leaves under elevated ultraviolet-B irradiation （UV-B, 280-320 nm）, the chloroplast ultrastructure of soybean seedlings was studied by hydroponics under laboratory conditions. The resuits showed that the thylakoid in chloroplast was orderly and clearly as soybean leaves were pretreated by La（Ⅲ）. The thylakoid was indis- tinctly disordered, expanded and even indiscoverable in the chloroplast under UV-B stress. The impact on the thylakoid by the high intensity UV-B irradiation （T2） was bigger than that by the low intensity UV-B irradiation （T1）. However, the destruction of the chloroplast structure caused by UV-B stress was alleviated by La（Ⅲ）, and the arrangement of the thylakoid in the chloroplast became orderly and clearly. The effect of the alleviation by La（Ⅲ） under the low intensity UV-B irradiation （T1） was better than that under the high intensity UV-B irradiation （T2）.
基金Project supported by the National Natural Science Foundation of China (20471030)the Foundation of State PlanningCommittee (IFZ20051210)
文摘The effect of La on flavonoids, chlorophyll contents, and phenylalanine ammonia-lyase （PAL） activity in soybean seedlings under supplementary ultraviolet-B radiation （UV-B, 280 - 320 nm） was studied. The results show that PAL activity, contents of flavonoids and chlorophyll in the plants pretreated with La （20 mg·L^- 1 ） are higher than those in CK. UV-B radiation could result in an increase in flavonoid content and PAL activity, associated with a decrease in chlorophyll content. However, the increase in the range of PAL activity and flavonoid content in UV-B treatment are lesser than those in the La treatment. The changes of flavonoid contents and PAL activity in La ＋ UV-B treatment are similar to those in UV-B treatment, and the increase in their range is higher than those in UV-B treatment. This shows that La can enhance the resistance of soybean seedling to UV-B radiation and alleviate the damage of UV-B radiation by increasing flavonoid content, chlorophyll content, and PAL activity.
文摘Wheat (Triticum aestivum), as a kind of important economic crop cultured in the Northern China, is affected by present-day enhanced ultraviolet-B (UV-B) radiation. To get the information of the impact by UV-B radiation on it, the proteins of wheat (Jin mai NO.8) leaves, which were divided into the normal light group (CK) and UV-B radiation group (B), were extracted and ran at SDS-PAGE at different treatment days (5, 6, 7). The proteins were also analyzed by run two-dimensional gel electrophoresis (2-DE), which allowed the identification of some significantly different gel spots. The proteins spots were further verified by Matrix-Assisted Laser Desorption/lonization-time of Flight Mass Spectrometry. The results showed: 1) the enhanced UV-B affects the growth of the wheat, as the visual changes appear on the sixth day;2) the proteins expressions between the B group and the CK group were remarkably different on the sixth day;3) the proteins of wheat leaves of the sixth day were further analyzed by 2-DE revealed that twenty-one protein points were identificated between the B group and the CK group. Among these twenty-one proteins, six proteins of them were up-regulated and twelve proteins of them were down-regulated, three new proteins were expressed only in the B group. Three proteins among six proteins, which were up-regulated, were further verified as RuBisCo large subunit binding protein;SOD;Calmodulin. The result indicates wheat could improve genes encoding proteins in their leaves and protect themselves, when enhanced UV-B affects the growth of the wheat.
文摘目的:探究窄谱中波紫外线联合0.1%他克莫司软膏治疗白癜风患者疗效及对皮损面积和色素积分的影响。方法:将笔者医院皮肤科于2020年1月-2023年1月收治的96例门诊患者纳为研究对象,根据随机数字法将所有患者分为对照组与观察组,每组各48例,给予对照组患者外涂0.1%他克莫司软膏治疗;观察组在对照组治疗基础上联合窄谱中波紫外线治疗。对比两组患者整体疗效,治疗前后皮损面积(Wood灯下皮损评估)与色素积分、不同部位严重性指数(Psoriasis area and severity index,PASI)与白癜风面积指数(Vitiligo area and severity index,VASI),不良反应发生情况及复发率。结果:观察组总有效率(97.92%)显著高于对照组(85.42%)(P<0.05);治疗后,两组患者皮损面积显著降低(P<0.05),且观察组显著低于对照组(P<0.05);治疗后,两组色素积分显著上升(P<0.05),且观察组显著高于对照组(P<0.05);治疗后,观察组各部位(躯干、四肢、面部)PASI与VASI均显著低于对照组(P<0.05);两组患者治疗期间不良反应发生率[观察组(16.67%) vs.对照组(10.42%)]与复发率[观察组(6.25%)vs.对照组(8.33%)]差异无统计学意义(P>0.05)。结论:窄谱中波紫外线联合0.1%他克莫司软膏可显著改善白癜风患者病情,缓解皮肤损伤,增加皮肤色素沉着,安全有效,值得临床推广。
