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石莼(Ulva lactuca L.)营养成分及多糖组成分析 被引量:6
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作者 林月 沈照鹏 +2 位作者 宗雯雯 崔欣 江晓路 《食品工业科技》 CAS 北大核心 2019年第17期304-308,313,共6页
为使人们对石莼的食用价值有更加深入的了解,扩展其消费市场,促进石莼资源的开发及高值化利用,本文对石莼的主要营养成分、氨基酸组成、部分微量元素含量进行了测定,同时对其主要活性成分石莼多糖进行了红外光谱与单糖组成分析。结果表... 为使人们对石莼的食用价值有更加深入的了解,扩展其消费市场,促进石莼资源的开发及高值化利用,本文对石莼的主要营养成分、氨基酸组成、部分微量元素含量进行了测定,同时对其主要活性成分石莼多糖进行了红外光谱与单糖组成分析。结果表明,石莼中蛋白质、碳水化合物、粗纤维、脂肪、灰分含量分别为(%):22.03、38.00、8.77、1.11、30.09,富含人体必需的Ca、Mg、Fe等微量元素,氨基酸种类齐全且必需氨基酸含量高达5.90 g/100 g;经红外光谱与高效液相色谱分析表明,从石莼提取出的石莼多糖的主要单糖组成为鼠李糖(45.23%)、木糖(26.04%)和葡萄糖(23.73%),且有糖醛酸与硫酸基存在。 展开更多
关键词 石莼 营养成分 多糖
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利用OJIP叶绿素a荧光评估干出对石莼(Ulva lactuca)光系统Ⅱ的影响 被引量:16
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作者 夏建荣 邹定辉 《海洋通报》 CAS CSCD 北大核心 2007年第4期50-55,共6页
通过分析快速叶绿素a荧光动力学上升(荧光快相)和JIP-test的参数探讨了干出对石莼光系统II特征的影响。结果表明,起始荧光(Fo)随水分的丧失明显增加,在失水达到67%时快速叶绿素a荧光动力学曲线出现一个平台,即O-J-I-P上升转变为O-J上升(... 通过分析快速叶绿素a荧光动力学上升(荧光快相)和JIP-test的参数探讨了干出对石莼光系统II特征的影响。结果表明,起始荧光(Fo)随水分的丧失明显增加,在失水达到67%时快速叶绿素a荧光动力学曲线出现一个平台,即O-J-I-P上升转变为O-J上升(J=I=P)。随着水分丧失的增加,以单位反应中心为单位的天线色素吸收的能量(ABS/RC)和用于热耗散的能量(DIo/RC)增加,当失水达到67%时,ABS/RC和DIo/RC分别达到起始时的127.5%和225.4%;用于电子传递的能量(ETo/RC)并没有明显变化;而捕获的能量(TRo/RC)在失水超过31%时明显下降。干出导致最大光化学效率(φPo)、电子传递的量子产额(φEo)和捕获的激子能导致电子传递的效率(ψo)均明显下降,在失水达到67%时,与未失水相比分别下降24.5%、86.4%和89.8%。而在J点的叶绿素a相对荧光(VJ)随着水分的丧失明显升高,同时单位面积上激活的反应中心数量明显减少。以上结果表明干出对石莼光合作用PSII的影响主要是由于PSII反应中心的失活和PSII受体侧电子传递的抑制。 展开更多
关键词 于出 叶绿素a荧光 光系统II 石莼
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Photosynthetic Responses to Inorganic Carbon in Ulva lactuca Under Aquatic and Aerial States
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作者 邹定辉 高坤山 《Acta Botanica Sinica》 CSCD 2002年第11期1291-1296,共6页
Intertidal macroalgae experience continual alternation of photosynthesis between aquatic state at high tide and aerial state at low tide. The comparative photosynthetic responses to inorganic carbon were investigated... Intertidal macroalgae experience continual alternation of photosynthesis between aquatic state at high tide and aerial state at low tide. The comparative photosynthetic responses to inorganic carbon were investigated in the common intertidal macroalga Ulva lactuca L. along the coast of Shantou between aquatic and aerial state. The inorganic carbon dissolved in seawater at present could fully (at 10 ℃ or 20 ℃) or nearly (at 30 ℃) saturate the aquatic photosynthesis of U. lactuca . However, the aerial photosynthesis was limited by current ambient atmospheric CO 2 level, and such a limitation was more severe at higher temperature (20-30 ℃) than at lower temperature (10 ℃). The carbon_saturated maximal photosynthesis of U. lactuca under aerial state was much greater than that under aquatic state at 10 ℃ and 20 ℃, while the maximal photosynthesis under both states was similar at 30 ℃. The aerial values of K m (CO 2) for photosynthesis were higher than the aquatic values. On the contrary, the values of apparent photosynthetic CO 2 conductance under aerial state were considerably lower than that under aquatic state. It was concluded that the increase of atmospheric CO 2 would enhance the primary productivity of U. lactuca through stimulating the photosynthesis under aerial state during low tide. 展开更多
关键词 ulva lactuca PHOTOSYNTHESIS inorganic carbon AQUATIC AERIAl atmospheric CO 2 rise
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Amphipods fail to suppress the accumulation of Ulva lactuca biomass in eutrophic Yundang Lagoon 被引量:1
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作者 ZHENG Xinqing HUANG Lingfeng +1 位作者 WANG Qian LIN Rongcheng 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2014年第12期155-162,共8页
The Gross growth efficiency (GGE) is defined as the amount of consumer carbon produced relative to the amount of prey ingested in a given time interval. In population ecology, it can be considered as an index for th... The Gross growth efficiency (GGE) is defined as the amount of consumer carbon produced relative to the amount of prey ingested in a given time interval. In population ecology, it can be considered as an index for the ability of a consumer to convert the biomass of food into its population production. In this study, it was firstly applied to estimate the role of amphipods grazing in the biomass dynamics of macroalgae (Ulva lactuca) in Yundang Lagoon (a subtropical eutrophic coastal lagoon, Xiamen City, China). It was found that amphipods were the dominant group (82.2%-98.7%) in the benthic assemblage, in which five amphipod species (Corophium uenoi, Ampithoe valida, Grandidierella japonica, Grammaropsis laevipalmata andCa-prella panantis) were observed in the lagoon. However, onlyA. valida significantly consumedU. lactuca among these amphipod species. A series of experiments were conducted to estimate the consumption of U. lactuca byA. valida. It was suggested that the monthly secondary production (P) ofA. valida using size frequency method ranged from 2.39 to 13.68 g/m2.GGEofA. valida exponentially reduced with body size and was closely associated with water temperature. Therefore,GGE equation for different months using the temperature recorded during the field investigation was fitted. The monthly consumption ofU. lactuca was figured out to be 86.7-1 549.0 g/m2 by the sum of consumption (P/GGE) byA. valida at different size, ac-counting for 2.0%-37.5% ofU. lactucaproduction. It indicates that the grazing effect ofA. valida did play a role in reducingthe accumulation ofU. lactucabiomass whenA. valida was abundant, but it finally fails to limit the U. lactucagrowth in Yundang Lagoon. Low abundance of A. valida and high nutrient loading may be two major reasons for the outbreak ofU. lactuca bloom in the lagoon. 展开更多
关键词 AMPHIPODS Ampithoe valida consumption gross growth efficiency secondary production ulva lactuca
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Antioxidative, cytotoxic, and anti-metastatic potentials of Laurencia obtusa and Ulva lactuca seaweeds
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作者 Reem Al Monla Yahya Salma +3 位作者 Achraf Kouzayha Hala Gali-Muhtasib Zeina Dassouki Hiba Mawlawi 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2021年第7期308-316,共9页
Objective:To investigate the antioxidant and cytotoxic properties of the most active extracts from Ulva lactuca and Laurencia obtusa against colon and cervical cancer cell lines.Methods:The antioxidant radical scaveng... Objective:To investigate the antioxidant and cytotoxic properties of the most active extracts from Ulva lactuca and Laurencia obtusa against colon and cervical cancer cell lines.Methods:The antioxidant radical scavenging activity of the algal extracts was estimated using the 2,2-diphenyl-1-picrylhydrazyl colorimetric assay.Moreover,the cytotoxic potential of these bioactive extracts was studied against HCT-116 and HeLa cells by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay.Wound healing analysis was adopted to evaluate the antimetastatic effects of protein extracts from both algae.Cell cycle analysis was performed by flow cytometry,and apoptotic bodies were detected using 4′,6-diamidino-2-phenylindole fluorescent staining.Amino acids of hydrolyzed protein extract were separated and identified chromatographically by high performance liquid chromatography.Results:The green algae Ulva lactuca had higher lipid content than Laurencia obtusa,whereas the latter had higher protein content with high antioxidant capacity.Protein extracts had significant dose-and time-dependent cytotoxicity and anti-metastatic activity against HCT-116 cells.Protein b extracts of both algae obtained from the chloroform:hexane solvent lipid-free residue caused morphological changes and induced apoptosis of HCT-116 cells.Further analysis revealed that apoptosis induced upon Laurencia obtusa protein b treatment was triggered via the ROS pathway,causing a significant increase in the sub-G_(1) population.Glycine and arginine(5.94 and 5.47μM,respectively)were the most common amino acids in Laurencia obtusa protein b extract.Conclusions:Proteins of Laurencia obtusa and Ulva lactuca could be considered as an adjuvant nutraceutical source of pro-apoptotic and anti-metastatic biomolecules against colon cancer. 展开更多
关键词 MACROAlGAE laurencia obtusa ulva lactuca Reactive oxygen species APOPTOSIS
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Nutrient and Nutraceutical Potentials of Seaweed Biomass Ulva lactuca and Kappaphycus alvarezii
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作者 R. G. Abirami S. Kowsalya 《Journal of Agricultural Science and Technology》 2011年第1期109-115,共7页
The study aimed at evaluating the nutrient and nutraceutical potentials of the selected seaweed biomass namely Ulva lactuca and Kappaphycus alvarezii. The selected seaweeds were found to be rich in macro and micronutr... The study aimed at evaluating the nutrient and nutraceutical potentials of the selected seaweed biomass namely Ulva lactuca and Kappaphycus alvarezii. The selected seaweeds were found to be rich in macro and micronutrients. Kappaphycus contained high amount of ash when compared to U. lactuca. It is revealed that the mercury level in U. lactuca was 0.017 ppm and it was not detected in K. alvarezii. The heavy metal concentration in the selected seaweeds was within the tolerable value reported as quality criteria for edible seaweeds. These seaweeds were also found to be rich sources of vitamin antioxidants namely Vitamin C, total carotenoid, beta carotene, total chlorophyll and polyphenol. The phytochemical screening of the selected seaweeds showed that both seaweeds contained carbohydrates, protein, gums and mulicage, phenols, starch and quinones. Alkaloids was absent in Kappaphycus, saponins was moderate in U. actuca when compared to Kappaphycus. Glycosides, flavonoids, tannins and terpenoids were absent in both the species. The selected seaweeds also possess antibacterial activity. The selected biomass could be recommended for use in dietaries to combat protein energy malnutrition and in particular micronutrient deficiencies. 展开更多
关键词 Seaweeds NUTRIENTS NUTRACEUTICAlS ulva lactuca Kappaphycus alvarezii
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TDZ对莴苣(Lactuca sativa L.)器官发生及乙烯形成的影响 被引量:7
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作者 徐华松 陆祖军 +1 位作者 王永繁 黄学林 《广西植物》 CAS CSCD 北大核心 1999年第1期92-94,共3页
为了研究TDZ在植物器官发生中的生理作用,用不同浓度的TDZ替代MS中的BA,发现仅用1/50BA浓度(0.1mg/L)的TDZ处理,已相当于用BA处理所得芽数,可见它具有很强的细胞分裂素活性;但是TDZ又能显著促进乙烯形成,较低浓度时抑制莴苣... 为了研究TDZ在植物器官发生中的生理作用,用不同浓度的TDZ替代MS中的BA,发现仅用1/50BA浓度(0.1mg/L)的TDZ处理,已相当于用BA处理所得芽数,可见它具有很强的细胞分裂素活性;但是TDZ又能显著促进乙烯形成,较低浓度时抑制莴苣的器官发生,可能此时乙烯起着主导作用。 展开更多
关键词 TDZ 莴苣 器官发生 乙烯
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Selenium distribution and nitrate metabolism in hydroponic lettuce(Lactuca sativa L.): Effects of selenium forms and light spectra 被引量:7
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作者 BIAN Zhong-hua LEI Bo +3 位作者 CHENG Rui-feng WANG Yu LI Tao YANG Qi-chang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第1期133-144,共12页
A deficiency in selenium(Se) in the human diet is a worldwide problem. The intake of Se-rich vegetables can be a safe way to combat Se deficiency for humans. However, most leafy vegetables can accumulate a high conten... A deficiency in selenium(Se) in the human diet is a worldwide problem. The intake of Se-rich vegetables can be a safe way to combat Se deficiency for humans. However, most leafy vegetables can accumulate a high content of nitrates, which poses a potential threat to human health. Light is an important environmental factor that regulates the uptake and distribution of mineral elements and nitrogen metabolism in plants. However, the effects of Se forms and light conditions, especially light spectra, on the uptake and translocation of Se and on nitrate reduction are poorly understood. In this study, lettuce(Lactuca sativa L.) was treated with exogenous Se applied as selenate(10 mmol L^-1) and selenite(0.5 mmol L^-1) and grown under five different light spectra: fluorescent light(FL), monochromatic red LED light(R), monochromatic blue LED light(B), and mixed red and blue LED light with a red to blue light ratio at 4(R/B=4), 8(R/B=8), and 12(R/B=12), respectively. The effects of light spectra and Se forms on plant growth, photosynthetic performance, Se accumulation and nitrate reduction were investigated. The results showed that the light spectra and Se forms had significant interactions for plant growth, foliar Se accumulation and nitrate reduction. The Se concentration and nitrate content in the leaves were negatively correlated with the percentage of red light from the light sources. Compared to Se applied as selenite, exogenous Se applied as selenate was more effective in reducing nitrate via promoting nitrate reductase and glutamate synthase activities. The lowest nitrate content and highest plant biomass were observed under R/B=8 for both the selenate and selenite treatments. The significant effect of the light spectra on the root concentration factor and translocation factor of Se resulted in marked variations in the Se concentrations in the roots and leaves. Compared with FL, red and blue LED light led to significant decreases in the foliar Se concentration. The results from this study suggest that the light spectra can contribute to Se distribution and accumulation to produce vegetables with better food quality. 展开更多
关键词 SElENIUM light spectra NITRATES nitrogen metabolism enzymes lEDs lactuca sativa l.
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Two new eudesmane sesquiterpenes from Lactuca sativa var.anagustata L 被引量:4
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作者 Yi Feng Han Gui Xiu Cao Min Xia 《Chinese Chemical Letters》 SCIE CAS CSCD 2009年第10期1211-1214,共4页
Two new eudesmane sesquiterpene lactones were isolated from the stalk of Lactuca sativa vat anagustata L and their structures were elucidated by means of spectroscopic methods, including 2D NMR (1H-1H COSY, HMBC and ... Two new eudesmane sesquiterpene lactones were isolated from the stalk of Lactuca sativa vat anagustata L and their structures were elucidated by means of spectroscopic methods, including 2D NMR (1H-1H COSY, HMBC and NOESY) as 1β-O-β-D- glucopyranosyl-4α-hydroxyl-5α, 6β, 11βH-eudesma-12, 6α-olide (1) and 1β-hydroxyl-15-O-(p-methoxyphenylacetyl)-5α, 6β, 11 βH-eudesma-3-en- 12, 6a-olide (2). 展开更多
关键词 lactuca sativa var. anagustata l COMPOSITAE Eudesmane sesquiterpene
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Effects of Priming in Combination with Fungicides on Germination and Infestation of Lettuce (Lactuca sativa L.) 被引量:2
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作者 HUJin TylkowskaK 《Agricultural Sciences in China》 CAS CSCD 2005年第6期449-454,共6页
Two seed lots of lettuce (Lactuca sativa L.) cv. Krlowa Majowych were primed in polyethylene glycol (PEG) 8000 at -1.25MPa for 7 days in dark at 20C. Two fungicide treatments combined with PEG were used to control fun... Two seed lots of lettuce (Lactuca sativa L.) cv. Krlowa Majowych were primed in polyethylene glycol (PEG) 8000 at -1.25MPa for 7 days in dark at 20C. Two fungicide treatments combined with PEG were used to control fungi: 1) Funaben T(0.1%) and Apron 35 SD (0.02%) were added to -1.25 MPa PEG solution (PEG+F); 2) After seed priming and drying,Funaben T was mixed at 5 g kg-1 and Apron 35 SD at 1 g kg-1 of seeds (PEG then F dust). PEG, PEG+F and PEG then F dusttreatments reduced significantly thermodormancy and increased the seed germination at 35C in both lots. PEG then Fdust treatment had the best effect on reducing seed thermodormancy at 35C, and increased seed germination to 64.5% inlot 1 and 72.0% in lot 2 compared to 0 in untreated. PEG and PEG then F dust treatments have accelerated germinationspeed at 20 and 15C in both lots. There was a significant reduction in seed infestation in which the seeds were treatedwith PEG+F and PEG then F dust compared to untreated seeds and PEG primed seeds. PEG priming enhanced seedinfestation considerably by Alternaria alternata, Penicillium spp., Rhizopus spp. and Ulocladium spp. Mycologicalanalysis showed higher frequency of Alternaria alternata and Ulocladium spp. in untreated seeds at 20C. Priming incombination with fungicides significantly alleviated the infestation of fungi, especially Alternaria alternata infestation. 展开更多
关键词 lactuca sativa l. PRIMING GERMINATION FUNGICIDE Mycological analysis Seed infestation
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不同LED组合光质对莴苣灰霉病防御酶活性的影响
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作者 刘勇鹏 王彬 +8 位作者 杨哲 任旭妍 朱新红 马肖静 孙凯乐 孙治强 朴凤植 张涛 姚秋菊 《山东农业科学》 北大核心 2023年第8期141-147,共7页
本试验在水培条件下,以LED红蓝光(红蓝光比例4∶1)为基础和对照,研究添加紫光、黄光、绿光组成的不同LED组合光质对叶用莴苣接种灰霉病菌后的病情指数以及接菌前后POD、PPO、PAL等相关防御酶活性的影响。结果表明,不同LED组合光质处理中... 本试验在水培条件下,以LED红蓝光(红蓝光比例4∶1)为基础和对照,研究添加紫光、黄光、绿光组成的不同LED组合光质对叶用莴苣接种灰霉病菌后的病情指数以及接菌前后POD、PPO、PAL等相关防御酶活性的影响。结果表明,不同LED组合光质处理中,以添加紫光的LED红蓝紫处理最能降低莴苣灰霉病病情指数;防御酶活性上,红蓝光基础上添加紫光和黄光处理的POD、PAL、PPO、GLU活性较高,添加绿光、黄光处理的CHT活性较高。整体上看,在LED红蓝光基础上添加一定比例的紫光或黄光,不仅可以有效降低莴苣灰霉病的病情指数,还可以提高莴苣体内的防御酶活性。该结果可为莴苣生产中通过光环境控制来抑制灰霉病发生提供理论参考。 展开更多
关键词 lED 组合光质 莴苣 灰霉病 防御酶
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Construction of Lactuca sativa Plastid Transformation Vector and High-level Expression of gfp Gene in Escherichia coli
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作者 Siming HOU Liying ZHOU +4 位作者 Lulu BU Chunlei YANG Ting GAO Tian TIAN Zheng'an YANG 《Agricultural Biotechnology》 CAS 2016年第4期1-4,共4页
Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragment... Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragments of exogenous gene were integrated to construct lettuce plastid expression vector p Brpo AGFP,which harbored the expression cassette Prrn-gfp-aad A-Tpsb A. The results showed that the amplified flanking fragments were 1.2 and 1.1 kb in size. After sequencing,restriction digestion,ligation and transformation,lettuce plastid expression vector containing expression cassette Prrn-gfp-aad A-Tpsb A was constructed and confirmed by SDS-PAGE electrophoresis. The results of SDS-PAGE electrophoresis indicated that gfp gene was efficiently expressed under the regulation of plasmid specific promoter Prrn and terminator Tpsb A. GFP accounted for 45. 6% of total soluble proteins; inclusion bodies accounted for 47.5 % of bacterial proteins,which reached relatively high expression levels. The construction of lettuce plastid expression vector p Brpo A-GFP laid a solid foundation for establishment of subsequent lettuce plastid transformation system and genetic improvement of lettuce using various functional genes. 展开更多
关键词 lactuca sativa var. capitata l. PlASTID Expression vector gfp gene High-level expression
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Effects of Different Concentrations of Photosynthetic Bacteria Solutions on Yield and Quality of Lactuca sativa L.
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作者 穆金艳 赵兰枝 王振宇 《Agricultural Science & Technology》 CAS 2017年第9期1649-1652,共4页
The effects of different concentrations of photosynthetic bacteria solutions (CK, 0 ml photosynthetic bacteria agent+4 000 ml nutrient solution; T1, 200 ml pho- tosynthetic bacteria agent+3 800 ml nutrient solution... The effects of different concentrations of photosynthetic bacteria solutions (CK, 0 ml photosynthetic bacteria agent+4 000 ml nutrient solution; T1, 200 ml pho- tosynthetic bacteria agent+3 800 ml nutrient solution; T2, 400 ml photosynthetic bacteria agent+3 600 ml nutrient solution; T3, 600 ml photosynthetic bacteria agent+ 3 400 ml nutrient solution; T4, 800 ml photosynthetic bacteria agent+3 200 ml nutrient solution; and T5, 1 000 ml photosynthetic bacteria agent+3 000 ml nutrient solu- tion) on the leaf number, fresh mass, root vigor, vitamin C content, soluble sugar content, soluble protein content and nitrate content of hydroponic Lactuca sativa L. were investigated. The results showed that the leaf number was increased by 62.66%, the fresh mass was increased by 139.7%, the root vigor was increased by 132.04%, the vitamin C content was increased by 18.34%, the soluble protein content was increased by 16.60%, the soluble sugar content was increased by 192.37%, and the nitrate content was reduced by 69.44% in the T3 group com- pared with those in the control group. The photosynthetic bacteria solution in the T3 group reduced the content of nitrate and improved the yield and quality of hydroponic L. sativa. 展开更多
关键词 Photosynthetic bacteria lactuca sativa l. NITRATE YIElD Quality
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Environmantal Risk Phytoaccumulation of Arsenic in Spinacea Olaracea L, and Lactuca Sativa L
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作者 Mirsad Veladzic Fatima Muhamedagie Osman Perviz Ekrem Pehlic Enez Selimbegovic 《Journal of Environmental Science and Engineering(B)》 2015年第7期368-371,共4页
Pollution of the environment, particularly the soil, appears to be one of the major contemporary issues. In addition, many studies point to the involvement of ecological risk of heavy metals into the food chain throug... Pollution of the environment, particularly the soil, appears to be one of the major contemporary issues. In addition, many studies point to the involvement of ecological risk of heavy metals into the food chain through edible plants like Spinaceae oleraceae L. and Lactuca sativa L.. This paper presents the results of the "ex-situ" application of phytoacumulation on the soils of contaminated terrain using the plant species of spinach (Spinacea oleracea L.) and lettuce (Lactuca sativa L.). Sequestration of arsen from the soil by these plant species was observed through phytoaccumulation factor--PF and limit values (mg/kg). The main task and objective of this research was to establish the level of accumulation of toxic element As from the soil by plants and to calculate the PF factor of transfer. The experiment was set up in the control conditions where the soil from eight contaminated locations was placed in the experimental containers. The AAS method was used to analyze heavy metal in plant material and soil. PF factor values ranged from 0.1 to 1.0 which indicates that the spinach and lettuce plants rechargeable moderate compared to arsenic. 展开更多
关键词 PHYTOACCUMUlATION Spinaceae oleraceae l. lactuca sativa l. arsenic.
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苦荬菜早熟(Lactuca indica L.)品种选育初报
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作者 张秀芬 吴渠来 王建光 《内蒙古农业大学学报(自然科学版)》 CAS 1984年第1期114-117,共4页
苦荚菜(Lactuca indica L.)为菊科蒿苣属一年生青刈饲料作物。1977年由河北省引入我区种植,表现高产、品质优良、适口性好。在呼市地区一年可刈制3~4次,亩产鲜草万斤左右。蛋旦质含量与苜蓿相近,脂肪含量却较高,纤维素含量低,是猪、禽... 苦荚菜(Lactuca indica L.)为菊科蒿苣属一年生青刈饲料作物。1977年由河北省引入我区种植,表现高产、品质优良、适口性好。在呼市地区一年可刈制3~4次,亩产鲜草万斤左右。蛋旦质含量与苜蓿相近,脂肪含量却较高,纤维素含量低,是猪、禽优良的青饲料。在呼市地区青饲期从六月下旬开始一直可延续至十月中下旬、所以深受群众欢迎。但是,苦荬菜在开始引入内蒙种植时,不能结实。其主要原因是:与引种地相比。 展开更多
关键词 开花结实 初报 农牧学院 内蒙古 品种选育 lactuca indica l 呼和浩特 鲜草产量 生育期 产草量
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石莼酶解提取物延缓皮肤衰老作用
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作者 黄瑾 江晓路 +5 位作者 余俊红 尹花 张洪峰 林月 詹照雅 沈照鹏 《食品工业科技》 CAS 北大核心 2024年第18期317-325,共9页
目的:研究石莼酶解提取物对D-半乳糖致衰老模型小鼠的抗衰老作用。方法:按800 mg/kg·d进行颈背部皮下注射D-半乳糖6周,建立小鼠亚急性衰老模型;提取物和V_(C)给药28 d后,表征小鼠皮肤老化及氧化应激水平。结果:与水浸提物相比,石... 目的:研究石莼酶解提取物对D-半乳糖致衰老模型小鼠的抗衰老作用。方法:按800 mg/kg·d进行颈背部皮下注射D-半乳糖6周,建立小鼠亚急性衰老模型;提取物和V_(C)给药28 d后,表征小鼠皮肤老化及氧化应激水平。结果:与水浸提物相比,石莼酶解提取物中可溶性总糖、蛋白质、还原糖、多酚固形物含量显著提高(P<0.05),且对羟自由基和DPPH自由基清除率及SOD活力提高5.72、7.95、13.26倍。动物实验显示,石莼酶解提取物显著改善衰老性皮肤GSH-Px、T-SOD、CAT、T-AOC活力及MDA、蛋白质羰基水平,缓解小鼠脏器萎缩;皮肤中胶原蛋白含量提高71.63%,Ⅰ/Ⅲ型胶原比例提高55.40%;显著提高机体抗氧化酶GSH-Px、T-SOD、CAT活力和T-AOC水平;抑制机体生物大分子的氧化降解,如降低蛋白质羰基和MDA水平(P<0.05)。结论:石莼酶解提取物可降低小鼠氧化应激水平,延缓小鼠皮肤衰老。 展开更多
关键词 石莼 酶解水提物 D-半乳糖 小鼠模型 皮肤衰老 抗氧化
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石莼发酵条件优化及其在刺参养殖中的应用效果
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作者 贾文广 李慧丽 +5 位作者 潘晓静 张洪霞 于贞 李蕊 邢荣莲 陈丽红 《大连海洋大学学报》 CAS CSCD 北大核心 2024年第3期410-419,共10页
为优化石莼(Ulva lactuca)发酵饲料工艺并探索石莼发酵饲料对刺参(Apostichopus japonicus)生长、消化性能的影响,选取特基拉芽孢杆菌(Bacillus tequilensis)作为发酵剂,以发酵产物中还原糖、游离氨基酸和多肽为评价指标,采用单因素试... 为优化石莼(Ulva lactuca)发酵饲料工艺并探索石莼发酵饲料对刺参(Apostichopus japonicus)生长、消化性能的影响,选取特基拉芽孢杆菌(Bacillus tequilensis)作为发酵剂,以发酵产物中还原糖、游离氨基酸和多肽为评价指标,采用单因素试验与响应面法对石莼发酵工艺条件进行优化,并进一步比较石莼发酵饲料、石莼未发酵干粉饲料和商品饲料对刺参生长性能、消化性能的影响。结果表明:石莼最佳发酵条件为温度33℃、接种菌量4.4×10^(6)CFU/g、含水率50%,此条件下发酵7 d,产物中游离氨基酸由3.6 mg/g增加到45.0 mg/g,多肽含量由30.1 mg/g增加到96.0 mg/g,还原糖含量由1.3 mg/g增加到20.8 mg/g;用3组饲料投喂刺参30 d后发现,刺参对3组饲料的摄食率无显著性差异(P>0.05),石莼发酵饲料组饲料转化率及消化酶活性均显著高于其他两个饲料组(P<0.05),刺参增重率与商品饲料组相差不大(P>0.05),但显著高于石莼未发酵干粉饲料组(P<0.05)。研究表明,石莼发酵饲料可替代商品饲料,不仅能提高刺参增重率、消化酶活性,同时也为刺参饲料的配制提供了广泛的原料资源。 展开更多
关键词 石莼 发酵饲料 工艺优化 刺参养殖 生长性能 消化性能
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叶用莴苣热激蛋白基因LsHsp70-2711的克隆及高温胁迫下的功能分析 被引量:21
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作者 李雅博 李婷 +1 位作者 韩莹琰 范双喜 《中国农业科学》 CAS CSCD 北大核心 2017年第8期1486-1494,共9页
【目的】通过克隆Hsp70相关基因,并利用VIGS分析叶用莴苣热胁迫下Hsp70表达量和形态变化,为解析热激蛋白基因Hsp70在高温胁迫下的响应机制及分子机理奠定基础。【方法】通过同源克隆及RACE技术,获得叶用莴苣热激蛋白LsHsp70-2711的cDNA... 【目的】通过克隆Hsp70相关基因,并利用VIGS分析叶用莴苣热胁迫下Hsp70表达量和形态变化,为解析热激蛋白基因Hsp70在高温胁迫下的响应机制及分子机理奠定基础。【方法】通过同源克隆及RACE技术,获得叶用莴苣热激蛋白LsHsp70-2711的cDNA全长序列,利用实时荧光定量PCR(qRT-PCR)分析该基因在不同温度和不同高温时间下的叶用莴苣热敏品种‘P-S11’和耐热品种‘G-S59’的表达差异,确定基因和高温的相关性。根据VIGS技术,构建p TRV-LsHsp702711瞬时沉默载体,转化农杆菌GV1301,注射法侵染叶用莴苣叶片,三周后经PCR鉴定得到阳性植株。对照组和阳性组在基因表达和形态上进行对比,对照植株和阳性植株热胁迫和干旱处理后再次分析LsHsp70-2711的表达特性,观测形态变化。【结果】LsHsp70-2711 cDNA全长为2 226 bp,开放阅读框为2 154 bp,编码718个氨基酸,与拟南芥(NP_187864.1)、番茄(NP_001266213.1)、水母雪莲花(AAB99745.1)等物种的Hsp70同源性达到80%以上,证明此基因属于Hsp70家族。根据qRT-PCR结果,高温胁迫下该基因在两个品种中的表达均上调,在耐热品种中总体表达水平均高于热敏品种,耐热品种LsHsp70-2711的表达量最大值出现在42℃、60 min,37℃、60 min时热敏品种基因表达量达最大值,且在42℃高温下热敏品种‘P-S11’中基因表达随着胁迫时间的增加受到抑制,而耐热品种‘G-S59’则能长时间保持较高的表达水平,此结果和田间两品种间耐热差异表现相符合。亚细胞定位显示,LsHsp70-2711主要在细胞质中。将构建好的载体侵入叶用莴苣,鉴定后获得阳性植株。由定量PCR结果可知,未进行胁迫处理的阳性植株与对照株相比,LsHsp70-2711表达量下降,茎长明显增长。热胁迫和干旱处理后的阳性植株LsHsp70-2711表达量显著低于对照植株,高温处理对LsHsp70-2711的影响大于干旱胁迫。【结论】LsHsp70-2711属于Hsp70基因家族,其与叶用莴苣耐热性相关。研究结果为解析叶用莴苣热激蛋白LsHsp70-2711在叶用莴苣高温抽薹方面的功能提供了理论支持。 展开更多
关键词 叶用莴苣 HSP70 基因克隆 高温胁迫 基因沉默
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高CO_(2)浓度下经济海藻羊栖菜与附生石莼的光合特性比较
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作者 曹聪 马增岭 +2 位作者 吴明江 徐丽丽 陈斌斌 《海洋渔业》 CSCD 北大核心 2024年第4期459-472,共14页
为了解大气CO_(2)浓度升高和附生杂藻竞争对羊栖菜(Sargassum fusiforme)生长与光合作用的影响,选择羊栖菜主要附生海藻石莼(Ulva lactuca)为对照,比较了在单独培养与共培养条件下两者的光合特性。结果显示,在单独培养条件下,高CO_(2)... 为了解大气CO_(2)浓度升高和附生杂藻竞争对羊栖菜(Sargassum fusiforme)生长与光合作用的影响,选择羊栖菜主要附生海藻石莼(Ulva lactuca)为对照,比较了在单独培养与共培养条件下两者的光合特性。结果显示,在单独培养条件下,高CO_(2)浓度提高了羊栖菜的最大电子传递速率(rETRm),降低了羊栖菜的暗呼吸速率(Rd)与总光合速率(Pg)的比值,但对石莼的Rd与Rd/Pg的比值无显著影响。瞬时高温(25℃)对羊栖菜的最大量子产率(Fv/Fm)和rETRm的抑制作用均显著高于石莼。在共培养条件下,羊栖菜的光合活性受到抑制,并显著低于石莼,而石莼的rETRm受到的影响并不显著;而且在共培养时,羊栖菜的Fv/Fm、rETRm和净光合速率(Pn)在瞬时高温(25℃)时均明显下降,而Rd达到最大,且其Q10值要高于石莼;瞬时温度变化对石莼rETRm无显著影响。研究表明,高CO_(2)浓度可以提高羊栖菜的光合作用,但是对石莼的影响不显著。羊栖菜光合作用与呼吸作用对温度变化更为敏感,而石莼有较强的适应温度变化的能力,特别是在共培养条件下表现出种间竞争优势。 展开更多
关键词 羊栖菜 石莼 光合作用 气候变化 海藻养殖
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不同红蓝LED光照强度对叶用莴苣生长和品质的影响 被引量:24
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作者 王志敏 宋非非 +2 位作者 徐志刚 刘晓英 杨杨 《中国蔬菜》 北大核心 2011年第16期44-49,共6页
以叶用莴苣品种永荣为试验材料,探讨了光照强度为100μmol·m-2·s-1(RB100)、200μmol·m-2·s-1(RB200)和300μmol·m-2·s-1(RB300)的红蓝LED光源对叶用莴苣生长与品质的影响,结果显示:与其他处理相比,RB10... 以叶用莴苣品种永荣为试验材料,探讨了光照强度为100μmol·m-2·s-1(RB100)、200μmol·m-2·s-1(RB200)和300μmol·m-2·s-1(RB300)的红蓝LED光源对叶用莴苣生长与品质的影响,结果显示:与其他处理相比,RB100处理叶用莴苣植株高度、叶面积、根长、比叶面积等形态指标及可溶性蛋白含量显著增加;RB300处理叶用莴苣中VC含量最高,硝酸盐和叶绿素含量最低。结果表明:300μmol·m-2·s-1光照强度有利于提高叶用莴苣的品质,而100μmol·m-2·s-1光照强度有利于叶用莴苣的生长。 展开更多
关键词 lED光源 光照强度 叶用莴苣 生长 品质
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