Congenital bilateral absence of the vas deferens （CBAVD） is a frequent cause of obstructive azoospermia, and mutations of the cystic fibrosis transmembrane conductance regulator （CFTR） gene have also been frequent...Congenital bilateral absence of the vas deferens （CBAVD） is a frequent cause of obstructive azoospermia, and mutations of the cystic fibrosis transmembrane conductance regulator （CFTR） gene have also been frequently identified in patients with CBAVD. However, the distribution of the CFTR polymorphisms M470V, poly-T, TG-repeats and F508del mutation in the Chinese CBAVD population with presumed low cystic fibrosis （CF） frequency remains to be evaluated. Samples obtained from 109 Chinese infertile males with CBAVD and 104 normal controls were analyzed for the presence of CFTR （TG）m（T）n, M470V and F508del by PCR amplification followed by direct sequencing. Our study showed that the F5OSdel mutation was not found in our patients. The 5T mutation was present with high frequency in Chinese CBAVD patients and IVS8-5T linked to either 12 or 13 TG repeats was highly prevalent among CBAVD patients （97.22% of 72 cases and 96.91% of 97 alleles with IVS8-5T）. Moreover, a statistically significant relationship between TG 12-5T-V470 haplotype and CBAVD was detected. This study indicated that the CFTR polymorphisms poly-T, TG-repeats and M470V might affect the process of CBAVD in the Chinese population.展开更多
This paper focused on the effect of pre-strain on forming limit curves(FLC)of 5754-O aluminum alloy sheet through utilizing biaxial tensile approach.Based on Swift model and Yld2000-2 d yield criterion,the dimensions ...This paper focused on the effect of pre-strain on forming limit curves(FLC)of 5754-O aluminum alloy sheet through utilizing biaxial tensile approach.Based on Swift model and Yld2000-2 d yield criterion,the dimensions of cruciform specimen was optimized through applying finite element method for increasing the strain at specimen center.After that,with the recommended specimen size,the cruciform specimen was tested under various stroke ratios to experimentally characterize the limit strains under different pre-strain levels.Subsequently,the biaxial tensile tests were simulated by Abaqus to obtain the limit strains and validate the material models.It can be observed in both experiments and simulations that the pre-strained uniaxial tension followed by plane tension or equi-biaxial tension can improve the formability of sheet metals.Besides,the strain path change affects the trend of first derivative of strain rate difference between neighboring points with respect to time.An early increase occurred and then fell back to the stable value,the steady evolution continued until to a new increase reaching the critical value.The M–K prediction approach was simulated to verify the influence of pre-strain on FLC.It can be found that the early increase peaks of the major strain incremental ratio rose with the amplitude of pre-strain.Finally,the phenomenon of pseudolocalization caused by the strain path change was explained through evolution of stress state inside the groove.展开更多
Micro machining has growing number of applications in various industries such as biomedical, automotive, aerospace, micro-sensor, micro-actuator and jewelry industries. Small-sized freeform titanium parts are frequent...Micro machining has growing number of applications in various industries such as biomedical, automotive, aerospace, micro-sensor, micro-actuator and jewelry industries. Small-sized freeform titanium parts are frequently needed in the biomedical applications, especially in the implantations such as mini-blood pumps and mini left-ventricular assist devices, finger joint replacements and small bone implants. Most of the small-sized titanium parts with freeform geometries are machined using micro ball-end milling before polishing and other surface treatments. Decreasing the cycle time of the machining parts is important for the productivity. In order to reduce the cycle time of the roughing process in the micro ball-end milling, this paper investigates the imple- mentation of a previously developed force-based feedrate scheduling （FFS） technique on micro milling of freeform titanium parts. After briefly introducing the instantaneous micro milling forces in micro ball-end milling of titanium parts with freeform surfaces, the FFS technique is implemented in the rough machining of a freeform titanium surface to demonstrate the cycle time reduction potentials via virtual micro milling simulations.展开更多
文摘Congenital bilateral absence of the vas deferens （CBAVD） is a frequent cause of obstructive azoospermia, and mutations of the cystic fibrosis transmembrane conductance regulator （CFTR） gene have also been frequently identified in patients with CBAVD. However, the distribution of the CFTR polymorphisms M470V, poly-T, TG-repeats and F508del mutation in the Chinese CBAVD population with presumed low cystic fibrosis （CF） frequency remains to be evaluated. Samples obtained from 109 Chinese infertile males with CBAVD and 104 normal controls were analyzed for the presence of CFTR （TG）m（T）n, M470V and F508del by PCR amplification followed by direct sequencing. Our study showed that the F5OSdel mutation was not found in our patients. The 5T mutation was present with high frequency in Chinese CBAVD patients and IVS8-5T linked to either 12 or 13 TG repeats was highly prevalent among CBAVD patients （97.22% of 72 cases and 96.91% of 97 alleles with IVS8-5T）. Moreover, a statistically significant relationship between TG 12-5T-V470 haplotype and CBAVD was detected. This study indicated that the CFTR polymorphisms poly-T, TG-repeats and M470V might affect the process of CBAVD in the Chinese population.
基金supported by the National Natural Science Foundation of China(Grant No.51875027)the China Postdoctoral Science Foundation(No.2018M630058)assistances of the program for the financial support。
文摘This paper focused on the effect of pre-strain on forming limit curves(FLC)of 5754-O aluminum alloy sheet through utilizing biaxial tensile approach.Based on Swift model and Yld2000-2 d yield criterion,the dimensions of cruciform specimen was optimized through applying finite element method for increasing the strain at specimen center.After that,with the recommended specimen size,the cruciform specimen was tested under various stroke ratios to experimentally characterize the limit strains under different pre-strain levels.Subsequently,the biaxial tensile tests were simulated by Abaqus to obtain the limit strains and validate the material models.It can be observed in both experiments and simulations that the pre-strained uniaxial tension followed by plane tension or equi-biaxial tension can improve the formability of sheet metals.Besides,the strain path change affects the trend of first derivative of strain rate difference between neighboring points with respect to time.An early increase occurred and then fell back to the stable value,the steady evolution continued until to a new increase reaching the critical value.The M–K prediction approach was simulated to verify the influence of pre-strain on FLC.It can be found that the early increase peaks of the major strain incremental ratio rose with the amplitude of pre-strain.Finally,the phenomenon of pseudolocalization caused by the strain path change was explained through evolution of stress state inside the groove.
文摘Micro machining has growing number of applications in various industries such as biomedical, automotive, aerospace, micro-sensor, micro-actuator and jewelry industries. Small-sized freeform titanium parts are frequently needed in the biomedical applications, especially in the implantations such as mini-blood pumps and mini left-ventricular assist devices, finger joint replacements and small bone implants. Most of the small-sized titanium parts with freeform geometries are machined using micro ball-end milling before polishing and other surface treatments. Decreasing the cycle time of the machining parts is important for the productivity. In order to reduce the cycle time of the roughing process in the micro ball-end milling, this paper investigates the imple- mentation of a previously developed force-based feedrate scheduling （FFS） technique on micro milling of freeform titanium parts. After briefly introducing the instantaneous micro milling forces in micro ball-end milling of titanium parts with freeform surfaces, the FFS technique is implemented in the rough machining of a freeform titanium surface to demonstrate the cycle time reduction potentials via virtual micro milling simulations.
