一年生二倍体簇毛麦携带多种抗性基因,被认为是小麦抗性改良的重要基因源。本研究前期创制了一个高抗小麦条锈病的普通小麦(中国春,CS)-簇毛麦3V(3D)代换系CD-3,并将该条锈病抗性基因初步定位于簇毛麦3V染色体上。本研究以川麦60作为父...一年生二倍体簇毛麦携带多种抗性基因,被认为是小麦抗性改良的重要基因源。本研究前期创制了一个高抗小麦条锈病的普通小麦(中国春,CS)-簇毛麦3V(3D)代换系CD-3,并将该条锈病抗性基因初步定位于簇毛麦3V染色体上。本研究以川麦60作为父本,以CD-3为母本,在回交自交系BC,F2群体中筛选到一个高抗条锈病的株系,命名为1901-245,并进一步对该株系进行分子标记、焚光原位杂交(FISH)及田间条绣病抗性鉴定。分子标记和熒光原位杂交(fluorescence in situ hybridization,FISH)结果显示,1901-245携带一对簇毛麦3V染色体,丢失了一对小麦3D染色体;1901-245花粉母细胞减数分裂中染色体配对正常,单价体出现频率较低,且3V染色体未出现单价体。田间条锈病抗性鉴定结果显示,1901-245及其亲本CD-3均表现为高抗小麦条锈病,而亲本川麦60则表现为高感小麦条锈病。以上结果表明,簇毛麦3V染色体上的条锈病抗性基因在1901-245的遗传背景下能正常表达;1901-245为普通小麦-簇毛麦3V(3D)代换系材料,高抗小麦条锈病且遗传稳定,可进一步在小麦遗传改良中加以应用。另外,FISH鉴定及植株形态观察结果显示,1901-245仍含有较多亲本CD-3(CS背景)的遗传背景,因此后期可继续将1901-245与川麦60进行回交,并在后代中加大簇毛麦3V染色体的追踪力度。展开更多
Co(en) 3V 3O 6·H 2O has been synthesized by using hydrothermal method and characterized by IR, ICP, XPS and X ray diffraction analysis. The results of the X ray diffraction analysis show that structure of Co(en) ...Co(en) 3V 3O 6·H 2O has been synthesized by using hydrothermal method and characterized by IR, ICP, XPS and X ray diffraction analysis. The results of the X ray diffraction analysis show that structure of Co(en) 3(VO 3) 3·H 2O is orthorhombic with P2(1)2(1)2(1) , and the crystal data are a =0 819 2(7) nm, b =1.263 9(1) nm, c =1.804 8(2) nm, V =1.868 8(3) nm 3, Z=4, D c=1.973 g/cm 3, F (000)=1 124, R 1=0.040 7, wR 2=0.094 9, ( Δ/ρ ) max =706 e/nm 3 and ( Δ/ρ ) min =-452 e/nm 3. The results of XPS show that the Co 2+ was oxidized to Co 3+ (en) 3 at about pH=7.0 in the aqueous solution.展开更多
文摘一年生二倍体簇毛麦携带多种抗性基因,被认为是小麦抗性改良的重要基因源。本研究前期创制了一个高抗小麦条锈病的普通小麦(中国春,CS)-簇毛麦3V(3D)代换系CD-3,并将该条锈病抗性基因初步定位于簇毛麦3V染色体上。本研究以川麦60作为父本,以CD-3为母本,在回交自交系BC,F2群体中筛选到一个高抗条锈病的株系,命名为1901-245,并进一步对该株系进行分子标记、焚光原位杂交(FISH)及田间条绣病抗性鉴定。分子标记和熒光原位杂交(fluorescence in situ hybridization,FISH)结果显示,1901-245携带一对簇毛麦3V染色体,丢失了一对小麦3D染色体;1901-245花粉母细胞减数分裂中染色体配对正常,单价体出现频率较低,且3V染色体未出现单价体。田间条锈病抗性鉴定结果显示,1901-245及其亲本CD-3均表现为高抗小麦条锈病,而亲本川麦60则表现为高感小麦条锈病。以上结果表明,簇毛麦3V染色体上的条锈病抗性基因在1901-245的遗传背景下能正常表达;1901-245为普通小麦-簇毛麦3V(3D)代换系材料,高抗小麦条锈病且遗传稳定,可进一步在小麦遗传改良中加以应用。另外,FISH鉴定及植株形态观察结果显示,1901-245仍含有较多亲本CD-3(CS背景)的遗传背景,因此后期可继续将1901-245与川麦60进行回交,并在后代中加大簇毛麦3V染色体的追踪力度。
文摘Co(en) 3V 3O 6·H 2O has been synthesized by using hydrothermal method and characterized by IR, ICP, XPS and X ray diffraction analysis. The results of the X ray diffraction analysis show that structure of Co(en) 3(VO 3) 3·H 2O is orthorhombic with P2(1)2(1)2(1) , and the crystal data are a =0 819 2(7) nm, b =1.263 9(1) nm, c =1.804 8(2) nm, V =1.868 8(3) nm 3, Z=4, D c=1.973 g/cm 3, F (000)=1 124, R 1=0.040 7, wR 2=0.094 9, ( Δ/ρ ) max =706 e/nm 3 and ( Δ/ρ ) min =-452 e/nm 3. The results of XPS show that the Co 2+ was oxidized to Co 3+ (en) 3 at about pH=7.0 in the aqueous solution.
