Biomimetic natural biomaterials for tissue engineering and regenerative medicine:new biosynthesis methods,recent advances,and emerging applications

Biomimetic materials have emerged as attractive and competitive alternatives for tissue engineering(TE)and regenerative medicine.In contrast to conventional biomaterials or synthetic materials,biomimetic scaffolds based on natural biomaterial can offer cells a broad spectrum of biochemical and biophysical cues that mimic the in vivo extracellular matrix(ECM).Additionally,such materials have mechanical adaptability,micro-structure interconnectivity,and inherent bioactivity,making them ideal for the design of living implants for specific applications in TE and regenerative medicine.This paper provides an overview for recent progress of biomimetic natural biomaterials(BNBMs),including advances in their preparation,functionality,potential applications and future challenges.We highlight recent advances in the fabrication of BNBMs and outline general strategies for functionalizing and tailoring the BNBMs with various biological and physicochemical characteristics of native ECM.Moreover,we offer an overview of recent key advances in the functionalization and applications of versatile BNBMs for TE applications.Finally,we conclude by offering our perspective on open challenges and future developments in this rapidly-evolving field.

OsFK1 encodes C-14 sterol reductase,which is involved in sterol biosynthesis and affects premature aging of leaves in rice

The enzyme C-14 sterol reductase is involved in biosynthesis of brassinosteroids(BR)and sterols,as well as plant development.OsFK1,a member of the sterol biosynthesis pathway located in the endoplasmic reticulum(ER),encodes C-14 sterol reductase.However,there is little research on the function of C-14 sterol reductase in rice.Compared with the wild type,an osfk1 mutant showed dwarf phenotype and premature aging in the second leaf during the trefoil stage,and abnormal development of leaf veins during the tillering stage.The osfk1 mutant showed signs of aberrant PCD,as evidenced by TUNEL staining.This suggested that high ROS buildup caused DNA damage and ROS-mediated cell death in the mutant.The osfk1 mutant also showed decreased chlorophyll content and aberrant chloroplast structure.Sequencing of the osfk1 mutant allele revealed a non-synonymous G to A mutation in the final intron,leading to early termination.Here,we identified the OsFK1 allele,cloned it by Mutmap sequencing,and verified it by complementation.HPLC-MS/MS assays demonstrated that the osfk1 mutation caused lower phytosterol levels.These findings showed that the OsFK1 allele encoding C-14 sterol reductase is involved in phytosterol biosynthesis and mediates normal development of rice plants.

Chain Elongation Using Native Soil Inocula:Exceptional n-Caproate Biosynthesis Performance and Microbial Mechanisms

This study demonstrates the feasibility and effectiveness of utilizing native soils as a resource for inocula to produce n-caproate through the chain elongation(CE)platform,offering new insights into anaerobic soil processes.The results reveal that all five of the tested soil types exhibit CE activity when supplied with high concentrations of ethanol and acetate,highlighting the suitability of soil as an ideal source for n-caproate production.Compared with anaerobic sludge and pit mud,the native soil CE system exhibited higher selectivity(60.53%),specificity(82.32%),carbon distribution(60.00%),electron transfer efficiency(165.00%),and conductivity(0.59 ms∙cm^(-1)).Kinetic analysis further confirmed the superiority of soil in terms of a shorter lag time and higher yield.A microbial community analysis indicated a positive correlation between the relative abundances of Pseudomonas,Azotobacter,and Clostridium and n-caproate production.Moreover,metagenomics analysis revealed a higher abundance of functional genes in key microbial species,providing direct insights into the pathways involved in n-caproate formation,including in situ CO_(2)utilization,ethanol oxidation,fatty acid biosynthesis(FAB),and reverse beta-oxidation(RBO).The numerous functions in FAB and RBO are primarily associated with Pseudomonas,Clostridium,Rhodococcus,Stenotrophomonas,and Geobacter,suggesting that these genera may play roles that are involved or associated with the CE process.Overall,this innovative inoculation strategy offers an efficient microbial source for n-caproate production,underscoring the importance of considering CE activity in anaerobic soil microbial ecology and holding potential for significant economic and environmental benefits through soil consortia exploration.

Rational Engineering of Secondary Metabolic Pathways in a Heterologous Host to Enable the Biosynthesis of Hibarimicin Derivatives with Enhanced Anti-Melanomic Activity

A 61-kb biosynthetic gene cluster(BGC),which is accountable for the biosynthesis of hibarimicin(HBM)B from Microbispora rosea subsp.hibaria TP-A0121,was heterologously expressed in Streptomyces coelicolor M1154,which generated a trace of the target products but accumulated a large amount of shunt products.Based on rational analysis of the relevant secondary metabolism,directed engineering of the biosynthetic pathways resulted in the high production of HBM B,as well as new HBM derivates with improved antitumor activity.These results not only establish a biosynthetic system to effectively synthesize HBMs-a class of the largest and most complex Type-Ⅱpolyketides,with a unique pseudo-dimeric structure-but also set the stage for further engineering and deep investigation of this complex biosynthetic pathway toward potent anticancer drugs.

Wetting alternating with partial drying during grain filling increases lysine biosynthesis in inferior rice grain

Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breeding and cultivation to improve nutritional quality.However,little information is available on differences in lysine anabolism between SG and IG and the underlying mechanism,and whether and how irrigation regimes affect lysine anabolism in these grains.A japonica rice cultivar was grown in the field and two irrigation regimes,continuous flooding(CF)and wetting alternating with partial drying(WAPD),were imposed from heading to the mature stage.Lysine content and activities of key enzymes of lysine biosynthesis,and levels of brassinosteroids(BRs)were lower in the IG than in the SG at the early grainfilling stage but higher at middle and late grain-filling stages.WAPD increased activities of these key enzymes,BR levels,and contents of lysine and total amino acids in IG,but not SG relative to CF.Application of 2,4-epibrassinolide to rice panicles in CF during early grain filling reproduced the effects of WAPD,but neither treatment altered the activities of enzymes responsible for lysine catabolism in either SG or IG.WAPD and elevated BR levels during grain filling increased lysine biosynthesis in IG.Improvement in lysine biosynthesis in rice should focus on IG.

Transcriptome analysis reveals steroid hormones biosynthesis pathway involved in abdominal fat deposition in broilers

Excessive abdominal fat deposition reduces the feed efficiency and increase the cost of production in broilers.Therefore,it is an important task for poultry breeders to breed broilers with low abdominal fat.Abdominal fat deposition is a highly complex biological process,and its molecular basis remains elusive.In this study,we performed transcriptome analysis to compare gene expression profiles at different stages of abdominal fat deposition to identify the key genes and pathways involved in abdominal fat accumulation.We found that abdominal fat weight(AFW)increased gradually from day 35(D35)to 91(D91),and then decreased at day 119(D119).Accordingly,after detecting differentially expressed genes(DEGs)by comparing gene expression profiles at D35 vs.D63 and D35 vs.D91,and identifying gene modules associated with fat deposition by weighted gene co-expression network analysis(WGCNA),we performed intersection analysis of the detected DEGs and WGCNA gene modules and identified 394 and 435 intersecting genes,respectively.The results of the Gene Ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses showed that the steroid hormone biosynthesis and insulin signaling pathways were co-enriched in all intersecting genes,steroid hormones have been shown that regulated insulin signaling pathway,indicating the importance of the steroid hormone biosynthesis pathway in the development of broiler abdominal fat.We then identified 6 hub genes(ACTB,SOX9,RHOBTB2,PDLIM3,NEDD9,and DOCK4)related to abdominal fat deposition.Further analysis also revealed that there were direct interactions between 6 hub genes.SOX9 has been shown to bind to proteins required for steroid hormone receptor binding,and RHOBTB2 indirectly regulates the steroid hormones biosynthesis through cyclin factor,and ultimately affect fat deposition.Our results suggest that the genes RHOBTB2 and SOX9 play an important role in fat deposition in broilers,by regulating steroid hormone synthesis.These findings provide new targets and directions for further studies on the mechanisms of fat deposition in chicken.

Identification and Molecular Characterization of the Alkaloid Biosynthesis Gene Family in Dendrobium catenatum

As one of the main active components of Dendrobium catenatum, alkaloids have high medicinal value. The physicochemicalproperties, conserved domains and motifs, phylogenetic analysis, and cis-acting elements of the genefamily members in the alkaloid biosynthesis pathway of D. catenatum were analyzed by bioinformatics, and theexpression of the genes in different years and tissues was analyzed by qRT-PCR. There are 16 gene families,including 25 genes, in the D. catenatum alkaloid biosynthesis pathway. The analysis of conserved domains andmotifs showed that the types, quantities, and orders of domains and motifs were similar among members ofthe same family, but there were significant differences among families. Phylogenetic analysis indicated that thegene family members showed some evolutionary conservation. Cis-acting element analysis revealed that therewere a large number of light-responsive elements and MYB (v-myb avian myeloblastosis viral oncogene homolog)-related elements in these genes. qRT-PCR showed that expressions of gene family members involved in alkaloidsynthesis were different in different years and tissues of D. catenatum. This study provides a theoretical basisfor further exploration of the regulatory mechanisms of these genes in the alkaloid biosynthesis of D. catenatum.

Research Progress on Functions and Biosynthesis of D-Psicose

D-Psicose,a naturally occurring rare sugar,exhibits a sweetness approximately 70%that of sucrose.It possesses high solubility,antioxidant activity,anti-inflammatory properties,and the ability to regulate cholesterol levels and enhance insulin sensitivity.However,D-psicose is relatively scarce in nature,making large-scale extraction and utilization impractical.Consequently,the development of cost-effective synthetic strategies for D-psicose is pivotal for its industrial application.In recent years,the Izumoring strategy has emerged as an efficient alternative to chemical synthesis for producing D-psicose.Nonetheless,limitations in the biotransformation of D-psicose,primarily governed by the conversion rate of D-psicose 3-epimerase(DPEase)and enzyme yield,continue to pose challenges in achieving economically viable production.Enzyme engineering and the establishment of high-level expression systems remain crucial avenues for reducing the overall biosynthesis costs.

The biosynthesis of alarm pheromone in the wheat aphid Rhopalosiphum padi is regulated by hormones via fatty acid metabolism

Aphids are major insect pests in agriculture and forestry worldwide.Following attacks by natural enemies,many aphids release an alarm pheromone to protect their population.In most aphids,the main component of the aphid alarm pheromone(AAP)is the sesquiterpene hydrocarbon(E)-β-farnesene(EβF).However,the mechanisms behind its biosynthesis and regulation remain poorly understood.In this study,we used the bird cherry–oat aphid Rhopalosiphum padi,which is an important wheat aphid,to investigate the regulatory mechanisms of EβF biosynthesis.Our results showed that EβF biosynthesis occurs during the mature embryo period and the molting period of the 1st-and 2nd-instar nymphs.Triglycerides provide the prerequisite material for EβF production and release.Based on transcriptome sequencing,RNAi analysis,hormone treatments,and quantitative measurements,we found that the biosynthesis of EβF utilizes acetyl coenzyme A produced from fatty acid degradation,which can be suppressed by juvenile hormone but it is promoted by 20-hydroxyecdysone through the modulation of fatty acid metabolism.This is the first systemic study on the modulation of EβF production in aphids.The results of our study provide insights into the molecular regulatory mechanisms of AAP biosynthesis,as well as valuable information for designing potential aphid control strategies.

Biosynthesis of Silver Nanoparticles with Clerodendron phlomoides Leave Extract:Particle Morphology,Antimicrobial Potential and Application

Silver nanoparticles are versatile nanomaterials that have found numerous applications in various fields.The use of plant extract for the synthesis of silver is a green and sustainable approach.Clerodendron phlomoides leaves extract has been found to contain various phytochemicals,such as phenols,flavonoids,tannins,and alkaloids,which possess reducing and stabilizing properties that can aid the production of silver particles.In this paper,morphological and topographical analyses were performed on silver nanoparticles.The biosynthesized silver nanoparticles showed antimicrobial potential against wound pathogens.SEM and TEM micrographs revealed that the particles were sphere and nanosized,which makes them suitable for various biomedical applications.

补肾活血序贯法对薄型子宫内膜大鼠内膜形态及VEGF、Vimentin、CK12蛋白表达的影响

目的:观察补肾活血序贯法中药对薄型子宫内膜大鼠内膜形态、血管内皮生长因子(VEGF)蛋白、波形蛋白(Vimentin)和组织细胞角蛋白12(cytokeratin 12,CK12)表达的影响。方法:将SPF级雌性SD大鼠连续灌胃羟基脲8 d复制肾虚薄型子宫内膜模型,造模成功后50只大鼠随机分为模型组、中药低剂量组、中剂量组、高剂量组及补佳乐组(阳性对照组)每组10只,另取10只大鼠为假手术组。假手术组和模型组灌胃等量蒸馏水,补佳乐组灌胃补佳乐0.3 mg/kg。其余3组动情期分别灌胃给予低、中、高剂量的消癥合剂;动情前期、动情后期和动情间期分别灌胃给予低、中、高剂量的养膜助孕包,连续给药3个动情周期,共15 d。干预结束后,HE染色观察大鼠子宫内膜形态;免疫组化检测大鼠子宫内膜VEGF、Vimentin和CK12表达。结果:模型组大鼠子宫内膜厚度、腺体数和血管数明显低于假手术组(P<0.01)。中药中、高剂量组及补佳乐组大鼠子宫内膜厚度、腺体数和血管数明显高于模型组(P<0.05或0.01)。中药中、高剂量组和补佳乐组大鼠子宫内膜组织VEGF的表达量均明显多于模型组(P<0.01),中药高剂量组子宫内膜组织Vimentin蛋白表达量明显高于模型组(P<0.01),中药低、中、高剂量组和补佳乐组子宫内膜组织CK12的表达明显多于模型组(P<0.05或0.01)。结论:补肾活血序贯法中药可以增加肾虚薄型子宫内膜模型大鼠的子宫内膜厚度及内膜组织VEGF、Vimentin、CK12蛋白表达。

益气养阴通络汤联合贝那普利治疗糖尿病肾病疗效及对血清TGF-β1、VEGF指标影响

目的探讨益气养阴通络汤联合贝那普利治疗糖尿病肾病(diabetic nephropathy,DN)的疗效及对转化生长因子-β_(1)(transforming growth factor-β_(1),TGF-β_(1)),血管内皮生长因子(vascular endothelial growth factor,VEGF)影响。方法选取2019年10月—2021年10月医院收治的DN病人94例,随机分为对照组(n=48)和观察组(n=46)。入院后两组病人均实施生活方式相关的指导,包括体质量控制、运动、戒烟酒等,给予脂质的代谢紊乱相关纠正、血糖控制、血压等基础的治疗,对照组实施盐酸贝那普利片的口服治疗,观察组则在此基础上实施益气养阴通络汤治疗,两组持续治疗4周。对比两组临床疗效、尿微量白蛋白(microalbumin,mALB)、尿白蛋白肌酐比(urine albumin creatine ratio,UACR)、β_(2)微球蛋白(β_(2)-microglobulin,β_(2)-MG)水平、中医证候评分、血清同型半胱氨酸(homocysteine,Hcy)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(Interleukin-6,IL-6)、血清TGF-β_(1)和VEGF及空腹血糖(fasting plasma glucose/fasting blood glucose,FPG)、餐后2 h血糖(2 hour postprandial blood glucose,2 h PG)和糖化血红蛋白(glyeosylated hemoglobin,HbAlc)指标变化。结果治疗前两组mALB、UACR、β_(2)-MG指标间无统计学意义(P>0.05);治疗后两组mALB、UACR、β_(2)-MG指标较治疗前显著降低(P<0.05);并且观察组降低较明显(P<0.05);观察组治疗总有效率较对照组明显较高(P<0.05);治疗后两组主症评分、次症评分和中医证候总评分明显降低(P<0.05);且观察组降低较明显(P<0.05);治疗后两组血清Hcy、TNF-α、IL-6指标明显降低(P<0.05);且观察组降低较明显(P<0.05);治疗后两组血清TGF-β_(1)和VEGF指标较治疗前明显降低(P<0.05);且观察组降低较明显(P<0.05);治疗后两组FPG、2 h PG和HbAlc指标较治疗前明显降低(P<0.05);且观察组降低较明显(P<0.05)。结论采用益气养阴通络汤联合贝那普利治疗DN具有较好的临床疗效,可降低血清TGF-β_(1),VEGF指标,降低蛋白尿水平。

高度近视继发黄斑新生血管抗VEGF术后患者近期视力预后相关因素分析

目的探讨影响抗血管内皮细胞生长因子(VEGF)治疗高度近视继发黄斑新生血管(MNV)患者近期视力预后的相关因素。方法回顾性分析2021年1月至2023年6月在安徽理工大学第一附属医院眼科接受抗VEGF治疗的高度近视继发MNV患者28例(28只眼)的临床资料。以术后1个月最佳矫正视力(BCVA)分为视力预后不良组和视力预后良好组,对比两组患者术前眼部特征及相关生物参数并进行统计学分析。结果高度近视继发MNV患者抗VEGF治疗1个月后BCVA、黄斑中心凹厚度(CMT)、MNV面积,较术前均显著改善,差异均有统计学差异(Z=6.371,6.949,2.437,均P<0.05)。单因素分析显示,两组患者在眼轴长度、后巩膜葡萄肿、CMT改善、MNV面积改善上差异均有统计学意义(均P<0.05)。多因素Logistic回归分析显示,眼轴长度和术前MNV面积是影响MNV患者抗VEGF治疗后视力预后的独立影响因素(均P<0.05)。结论抗VEGF治疗高度近视继发MNV可有效提高患者视力,术后视力与眼轴长度、后巩膜葡萄肿、CMT改善、MNV面积改善相关,其中,眼轴长度和术前MNV面积是影响视力预后的独立影响因素。

Cyclin D1、VEGF联合NLR、PLR水平预测慢性鼻窦炎-鼻息肉术后复发的临床研究

目的探究Cyclin D1、血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)表达含量联合中性粒细胞/淋巴细胞比值(Neutrophil-lymphocyte Ratio,NLR)和血小板/淋巴细胞比值(Platelet-lymphocyte Ratio,PLR)水平预测慢性鼻窦炎伴鼻息肉(Chronic Rhinosinusitis with Nasal Polyps,CRSwNP)复发的临床加价值。方法选取广东省韶关市粤北人民医院耳鼻喉科于2021年1月—2022年6月收治的333例CRSwNP患者为研究对象,均行慢性鼻窦炎-鼻息肉术治疗,根据术后复发情况分为复发组(138例)和未复发组(195例),比较两组患者一般资料、Cyclin D1、VEG、NLR、PLR水平,采用受试者工作特征(Receiver Operating Characteristic,ROC)曲线分析Cyclin D1、VEGF联合NLR、PLR水平对CRSwNP者术后1年复发的预测效能。结果两组Cyclin D1、VEGF、NLR、PLR水平对比,差异有统计学意义(P均<0.05)。Logistic回归分析结果显示,CRSwNP患者复发的影响因素包括Cyclin D1、VEGF、NLR、PLR水平(OR=1.521、1.483、1.365、1.403,P均<0.05)。ROC曲线结果显示Cyclin D1、VEGF、NLR、PLR四者联合检测的灵敏度、特异度及曲线下面积均高于单独检测(P均<0.05)。结论Cyclin D1、VEGF、NLR、PLR水平是诱发CRSwNP患者术后复发的相关因素,也是预测CRSwNP患者术后复发的可靠性指标。

抗VEGF药物联合全视网膜光凝治疗糖尿病视网膜病变的效果和预后分析

目的探讨抗血管内皮生长因子(vascular endothelial growth factor,VEGF)药物联合全视网膜光凝(panretinal photocoagulation,PRP)治疗糖尿病视网膜病变的效果和预后分析。方法选取三明市第二医院2020年1月—2023年1月收治的50例糖尿病视网膜病变者(78眼),以随机数字表法分为2组,各25例(39眼)。对照组患者给予PRP治疗,研究组给予抗VEGF药物联合PRP治疗。比较2组术前、术后恢复指标,统计患者治疗后的不良反应,评价治疗效果。结果术后,2组最佳矫正视力(best correct vision acuity,BCVA)优于术前,视网膜新生血管(retinal neovascularization,RNV)面积、中央视网膜厚度(central retinal thickness,CRT)低于术前,且研究组BCVA高于对照组;RNV面积、CRT低于对照组,差异有统计学意义(P<0.05);研究组并发症总发生率为5.13%,低于对照组的23.08%,差异有统计学意义(P<0.05);研究组治疗总有效率为94.97%,高于对照组的79.49%,差异有统计学意义(P<0.05)。结论糖尿病视网膜病变以抗VEGF药物联合PRP治疗可改善术后BCVA,减少RNV面积、CRT,降低并发症发生率,提升治疗效果。

小檗碱调节HIF-1α/VEGF信号通路对皮肤溃疡大鼠血管生成和创面愈合的影响

目的:探究小檗碱(BR)调节缺氧诱导因子-1α(HIF-1α)/血管内皮生长因子(VEGF)信号通路对皮肤溃疡大鼠血管生成和创面愈合的影响。方法:取SD大鼠采用创面局部多次涂抹冰醋酸建立皮肤溃疡模型,随机数字法分组,包括模型组(M组)、小檗碱低剂量组(BR-L组)、小檗碱高剂量组(BR-H组)、小檗碱高剂量+空载组(BR-H+pc-DNA组)、小檗碱高剂量+HIF-1α敲低组(BR-H+pc-HIF-1αKD组),每组10只,另选10只SD大鼠作为对照组(C组)。以药物分组处理3周后,测定各组大鼠创面愈合率;HE染色检测各组大鼠皮肤溃疡创面组织病理形态;免疫组织化学染色检测各组大鼠皮肤溃疡创面组织微血管密度(MVD)及胶原蛋白Ⅰ(CollagenⅠ)与纤维结合蛋白(FN)表达;酶联免疫吸附测定(ELISA)各组大鼠血清及皮肤溃疡创面组织炎性因子IL-6、IL-8表达水平;免疫印迹法检测各组大鼠皮肤溃疡创面组织HIF-1α/VEGF通路蛋白表达水平。结果:与C组相比,M组大鼠创面组织MVD、血清及创面组织IL-6、IL-8水平升高(均P<0.05)。与M组相比,BR-L组、BR-H组、BR-H+pc-DNA组大鼠创面愈合率、创面组织MVD、HIF-1α及VEGF蛋白表达均升高(均P<0.05),血清及创面组织IL-6、IL-8水平均降低(均P<0.05),且高剂量小檗碱作用更强。敲低HIF-1α可减弱BR对模型组大鼠各指标的作用。结论:小檗碱可通过上调HIF-1α/VEGF通路而降低炎性因子表达,抑制炎症,增强皮肤溃疡大鼠血管生成和胶原形成,促进其创面愈合。

VEGF和SOX2在卵巢癌中的表达及与病人预后的关系研究

目的 探讨血管内皮生长因子(VEGF)和Y染色体性别决定区相关的高迁移率族盒蛋白2(SOX2)在卵巢癌中的表达及与病人预后的关系。方法 筛选收集病理科存档的卵巢癌手术或穿刺标本,对筛选收集的患者进行随访,获取随访资料,查阅病历,获取临床资料,采用免疫组织化学方法检测46例晚期卵巢癌、28例早期卵巢癌及20例正常卵巢组织手术或穿刺标本中VEGF、SOX2的表达情况,观察上述指标的阳性表达差异及相关性,并分析其与卵巢癌患者临床病理参数及生存资料的关系。结果 不同标本中的VEGF、SOX2阳性表达率相比,晚期卵巢癌>早期卵巢癌>正常卵巢组织(P<0.05)。卵巢癌标本中VEGF与SOX2的表达有正相关性(P<0.05)。在淋巴结转移患者中,VEGF、SOX2阳性表达率明显上升,且在III~IV期患者中,VEGF阳性表达率明显上升(P<0.05)。VEGF、SOX2同时阳性者的OS明显短于VEGF、SOX2同时阴性者(P<0.05)。结论 VEGF、SOX2在卵巢癌尤其是晚期卵巢癌中均呈高表达状态,且两者呈正相关,VEGF在淋巴结转移、高分期患者中表达更高,而SOX2在淋巴结转移患者中表达更高,两者的高表达均可能提示患者预后不佳。

脑胶质瘤患者脑脊液中VEGF、HGF及PDGF水平与术后复发的相关性

目的探讨胶质瘤患者脑脊液中血管内皮生长因子(vascular endothelial growth factor,VEGF)、肝细胞生长因子(hepatocyte growth factor,HGF)及血小板衍生生长因子(platelet-derived growth factor receptor,PDGF)的表达及其与患者术后复发的相关性。方法选取70例脑胶质瘤患者为研究对象,采用酶联免疫吸附法分析脑脊液中VEGF、HGF及PDGF的水平,术后随访6个月患者复发情况,将患者分为复发组和未复发组,分析脑脊液中细胞因子水平与复发的相关性。结果低级别胶质瘤患者脑脊液中VEGF、HGF及PDGF水平分别为(22.13±2.87)pg/ml、(515.30±209.10)pg/ml及(79.23±6.79)pg/ml,高级别胶质瘤患者脑脊液中VEGF为(30.22±2.82)pg/ml、HGF为(1194.00±354.50)pg/ml及PDGF为(97.89±7.40)pg/ml,显著高于低级别胶质瘤患者(P<0.001)。胶质瘤患者脑脊液中VEGF、HGF及PDGF水平两两之间呈显著正相关(P<0.001)。二元logistic回归分析表明患者脑脊液中VEGF及HGF水平为胶质瘤患者术后复发的独立影响因素(P<0.05),受试者工作特征(ROC)曲线显示VEGF与HGF联合对胶质瘤术后复发具有一定的预测效能(曲线下面积:0.856;P<0.001)。结论胶质瘤患者脑脊液中VEGF、HGF及PDGF水平显著升高,脑脊液VEGF及HGF水平为术后复发的独立影响因素,且两者联合预测术后复发具有一定的价值。

益母草注射液通过其生物碱成分上调Tie-2、VEGFR2和VEGF促进斑马鱼血管新生

目的:研究益母草注射液在斑马鱼血管损伤模型中的促血管生成作用,并阐明潜在的物质基础和分子机制。方法:采用HPLC-PAD和TLC技术检测益母草注射液的主要成分;利用舒尼替尼诱导转基因Tg(flk1:eGFP)斑马鱼血管损伤模型,益母草注射液干预治疗后,测量斑马鱼节间血管(ISVs)长度,QT-PCR检测血管生成相关基因的表达水平。结果:发现益母草注射液的主要成分是水苏碱、胆碱和葫芦巴碱;其中,益母草注射液、葫芦巴碱和胆碱可减轻化学诱导的斑马鱼血管损伤,其作用机制涉及益母草注射液上调Tie-2、VEGF和VEGFR2的表达,其主要成分葫芦巴碱上调Tie-2和VEGF的表达,胆碱上调VEGF和VEGFR2的表达。结论:益母草注射液及其主要生物碱成分可通过上调Tie-2、VEGFR2和VEGF来促进血管生成。

超声乳化联合玻璃体注射抗VEGF药治疗白内障合并黄斑水肿的应用

目的观察白内障超声乳化联合玻璃体腔注射抗血管内皮生长因子(VEGF)药物治疗白内障合并各种黄斑水肿的临床效果。方法回顾性病例对照研究。选取眼科2019年1月至2022年12月期间眼科住院白内障合并各种黄斑病变(糖尿病性黄斑水肿,年龄相关性黄斑变性),高度近视脉络膜新生血管(CNV)20例(20只眼),随机分成两组,A组(10例)给予白内障超声乳化吸除联合人工晶状体植入术,术中联合玻璃体内抗VEGF药物注射,B组(10例)行白内障超声乳化联合人工晶状体植入术。随访1个月,观察两组之间治疗前后:前房及玻璃体情况、最佳矫正视力(BCVA)、黄斑中心凹厚度(CMT)、眼压的差异。结果A组术后CMT及眼压较术前降低,和B组比较差异有统计学意义(P<0.05)。两组术后BCVA都较术前提高,A组明显好于B组,差异有统计学意义(P<0.05)。结论白内障超声化术联合玻璃体抗VEGF药物,治疗白内障合并黄斑水肿应用安全有效,值得推广。