通过数值计算,详细研究了射流偏转角与主流夹角大于90°的逆主流小孔稳态射流(Reversed in jectionVG Js)对低雷诺数涡轮流动分离的控制。研究结果发现,逆主流射流对主流的扰动引起射流孔后边界层迅速转捩可抑制流动分离现象。射流...通过数值计算,详细研究了射流偏转角与主流夹角大于90°的逆主流小孔稳态射流(Reversed in jectionVG Js)对低雷诺数涡轮流动分离的控制。研究结果发现,逆主流射流对主流的扰动引起射流孔后边界层迅速转捩可抑制流动分离现象。射流作为"湍流发生器"从控制机理上有别于90°偏转角VG Js射流状态。高射流湍流度(10%),135°逆主流VG Js在达到与90°偏转角VG Js基本相同的流动分离控制效果时,可降低射流流量67%。展开更多
To develop vortex generator jet （VGJ） method for flow control, the turbulence flow in a 14° conical diffuser with and without vortex generator jets are simulated by solving Navier-Stokes equations with k-ε tur...To develop vortex generator jet （VGJ） method for flow control, the turbulence flow in a 14° conical diffuser with and without vortex generator jets are simulated by solving Navier-Stokes equations with k-ε turbulence model. The diffuser performance, based on different velocity ratio （ratio of the jet speed to the mainstream velocity）, is investigated and compared with the experimental study. On the basis of the flow characteristics using computation fluid dynamics （CFD） method observed in the conical diffuser and the downstream development of the longitudinal vortices, attempt is made to correlate the pressure recovery coefficient with the behavior of vortices produced by vortex generator jets.展开更多
Vibrio cholerae(V. cholerae) genome is equipped with a number of integrative mobile genetic element(IMGE) like prophages, plasmids, transposons or genomic islands, which provides fitness factors that help the pathogen...Vibrio cholerae(V. cholerae) genome is equipped with a number of integrative mobile genetic element(IMGE) like prophages, plasmids, transposons or genomic islands, which provides fitness factors that help the pathogen to survive in changing environmental conditions. Metagenomic analyses of clinical and environmental V. cholerae isolates revealed that dimer resolution sites(dif) harbor several structurally and functionally distinct IMGEs. All IMGEs present in the dif region exploit chromosomally encoded tyrosine recombinases, Xer C and Xer D, for integration. Integration takes place due to site-specific recombination between two specific DNA sequences; chromosomal sequence is called att B and IMGEs sequence is called att P. Different IMGEs present in the att P region have different attP structure but all of them are recognized by Xer C and Xer D enzymes and mediate either reversible or irreversible integration. Cholera toxin phage(CTXΦ), a lysogenic filamentous phage carrying the cholera toxin genes ctx AB, deserves special attention because it provides V. cholerae the crucial toxin and is always present in the dif region of all epidemic cholera isolates. Therefore, understanding the mechanisms of integration and dissemination of CTXΦ, genetic and ecological factors which support CTXΦ integration as well as production of virion from chromosomally integrated phage genome and interactions of CTXΦ with other genetic elements present in the genomes of V. cholerae is important for learning more about the biology of cholera pathogen.展开更多
基金This project is supported by Scientific Research Foundation of Ministry of Education of China for Returnee.
文摘To develop vortex generator jet （VGJ） method for flow control, the turbulence flow in a 14° conical diffuser with and without vortex generator jets are simulated by solving Navier-Stokes equations with k-ε turbulence model. The diffuser performance, based on different velocity ratio （ratio of the jet speed to the mainstream velocity）, is investigated and compared with the experimental study. On the basis of the flow characteristics using computation fluid dynamics （CFD） method observed in the conical diffuser and the downstream development of the longitudinal vortices, attempt is made to correlate the pressure recovery coefficient with the behavior of vortices produced by vortex generator jets.
基金Supported by Research in the Laboratory of Das B and NairGB is funded in part by Department of Science Technology,No.SB/FT/LS-309/2012Government of India(GOI)and the Department of Biotechnology,No.BT/MB/THSTI/HMC-SFC/2011Research in the Laboratory of Bhadra RK is partly financiallysupported by Council of Scientific and Industrial Research,GOIand Indian Council of Medical Research,GOI
文摘Vibrio cholerae(V. cholerae) genome is equipped with a number of integrative mobile genetic element(IMGE) like prophages, plasmids, transposons or genomic islands, which provides fitness factors that help the pathogen to survive in changing environmental conditions. Metagenomic analyses of clinical and environmental V. cholerae isolates revealed that dimer resolution sites(dif) harbor several structurally and functionally distinct IMGEs. All IMGEs present in the dif region exploit chromosomally encoded tyrosine recombinases, Xer C and Xer D, for integration. Integration takes place due to site-specific recombination between two specific DNA sequences; chromosomal sequence is called att B and IMGEs sequence is called att P. Different IMGEs present in the att P region have different attP structure but all of them are recognized by Xer C and Xer D enzymes and mediate either reversible or irreversible integration. Cholera toxin phage(CTXΦ), a lysogenic filamentous phage carrying the cholera toxin genes ctx AB, deserves special attention because it provides V. cholerae the crucial toxin and is always present in the dif region of all epidemic cholera isolates. Therefore, understanding the mechanisms of integration and dissemination of CTXΦ, genetic and ecological factors which support CTXΦ integration as well as production of virion from chromosomally integrated phage genome and interactions of CTXΦ with other genetic elements present in the genomes of V. cholerae is important for learning more about the biology of cholera pathogen.
