Objective To investigate the antitumor effects of VnA,the total alkaloids isolated from Veratrum Nigrum L.var.ussuriense Nakai("Wusuli Lilu" in Chinese),and the underlying mechanisms with emphasis on its ant...Objective To investigate the antitumor effects of VnA,the total alkaloids isolated from Veratrum Nigrum L.var.ussuriense Nakai("Wusuli Lilu" in Chinese),and the underlying mechanisms with emphasis on its anti-metastatic effects.Methods The effects of VnA on in vitro proliferation,invasion,migration and adhesion in Colon26-L5 cells were investigated.The effect of VnA on experimental lung metastasis of Colon26-L5 cells in mice was also be studied by means of measuring the numbers of tumor colonies in lungs after single i.v.administration of Colon26-L5 cells to mice followed by q.d.i.p VnA for consecutive 14 days.The effect of VnA on the production of matrix metalloproteinases(MMPs)from Colon26-L5 cell in vitro was determined by means of gelatin zymography.Results In in vitro experiments,VnA was found to significantly reduce the number of tumor colonies at dosage of 20.0-40.0 μg·kg-1.In in vitro experiments,VnA inhibited the adhesion(at 1.6-12.5 μg·mL-1)and migration(at 3.1-50.0 μg·mL-1)of Colon26-L5 cell to extracellular matrix components and suppressed invasion into reconstituted basement membrane matrigel(at 3.1-50.0 μg·mL-1),meanwile cell proliferation(at 25.0-50.0 μg·mL-1)was attenuated.VnA also showed a concentration-dependent inhibition of MMP2 and MMP9 production(at 3.1-50.0 μg·mL-1).Conclusions VnA has anti-metastatic protential by decreasing invasiveness of cancer cells as one of its anti-tumor pharmacological effects.展开更多
文摘Objective To investigate the antitumor effects of VnA,the total alkaloids isolated from Veratrum Nigrum L.var.ussuriense Nakai("Wusuli Lilu" in Chinese),and the underlying mechanisms with emphasis on its anti-metastatic effects.Methods The effects of VnA on in vitro proliferation,invasion,migration and adhesion in Colon26-L5 cells were investigated.The effect of VnA on experimental lung metastasis of Colon26-L5 cells in mice was also be studied by means of measuring the numbers of tumor colonies in lungs after single i.v.administration of Colon26-L5 cells to mice followed by q.d.i.p VnA for consecutive 14 days.The effect of VnA on the production of matrix metalloproteinases(MMPs)from Colon26-L5 cell in vitro was determined by means of gelatin zymography.Results In in vitro experiments,VnA was found to significantly reduce the number of tumor colonies at dosage of 20.0-40.0 μg·kg-1.In in vitro experiments,VnA inhibited the adhesion(at 1.6-12.5 μg·mL-1)and migration(at 3.1-50.0 μg·mL-1)of Colon26-L5 cell to extracellular matrix components and suppressed invasion into reconstituted basement membrane matrigel(at 3.1-50.0 μg·mL-1),meanwile cell proliferation(at 25.0-50.0 μg·mL-1)was attenuated.VnA also showed a concentration-dependent inhibition of MMP2 and MMP9 production(at 3.1-50.0 μg·mL-1).Conclusions VnA has anti-metastatic protential by decreasing invasiveness of cancer cells as one of its anti-tumor pharmacological effects.
