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Characteristics and pathological mechanism on magnetic resonance diffusion-weighted imaging after chemoembolization in rabbit liver VX-2 tumor model 被引量:14
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作者 You-Hong Yuan En-Hua Xiao +6 位作者 Jian-Bin Liu Zhong He Ke Jin Cong Ma Jun Xiang Jian-Hua Xiao Wei-Jian Chen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第43期5699-5706,共8页
AIM: To investigate dynamic characteristics and pathological mechanism of signal in rabbit VX-2 tumor model on diffusion-weighted imaging (DWI) after chemoembolization. METHODS: Forty New Zealand rabbits were included... AIM: To investigate dynamic characteristics and pathological mechanism of signal in rabbit VX-2 tumor model on diffusion-weighted imaging (DWI) after chemoembolization. METHODS: Forty New Zealand rabbits were included in the study and forty-seven rabbit VX-2 tumor models were raised by implanting directly and intrahepatically after abdominal cavity opened. Forty VX-2 tumor models from them were divided into four groups. DWI was performed periodically and respectively for each group after chemoembolization. All VX-2 tumor samples of each group were studied by pathology. The distinction of VX-2 tumors on DWI was assessed by their apparent diffusion coefficient (ADC) values. The statistical significance between different time groups, different area groups or different b-value groups was calculated by using SPSS12.0 software. RESULTS: Under b-value of 100 s/mm2, ADC values were lowest at 16 h after chemoembolization in area of VX-2 tumor periphery, central, and normal liver parenchyma around tumor, but turned to increase with further elongation of chemoembolization treatment. The distinction of ADC between different time groups was significant respectively (F = 7.325, P < 0.001; F = 2.496, P < 0.048; F = 6.856, P < 0.001). Cellular edema in the area of VX-2 tumor periphery or normal liver parenchyma around tumor, increased quickly in sixteen h after chemoembolization but, from the 16th h to the 48th h, cellular edema in the area of normal liver parenchyma around tumor decreased gradually and that in the area of VX-2 tumor periphery decreased lightly at, and then increased continually. After chemoembolization, Cellular necrosis in the area of VX-2 tumor periphery was more significantly high than that before chemoembolization. The areas of dead cells in VX-2 tumors manifested low signal and high ADC value, while the areas of viable cells manifested high signal and low ADC value. CONCLUSION: DWI is able to detect and differentiate tumor necrotic areas from viable cellular areas before and after chemoembolization. ADC of normal liver parenchyma and VX-2 tumor are influenced by intracellular edema, tissue cellular death and microcirculation disturbance after chemoembolization. 展开更多
关键词 LIVER vx-2 tumor Diffusion-weighted imaging Apparent diffusion coefficient CHEMOEMBOLIZATION
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Characteristics of liver on magnetic resonance diffusion-weighted imaging:Dynamic and image pathological investigation in rabbit liver VX-2 tumor model 被引量:10
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作者 You-Hong Yuan En-Hua Xiao +6 位作者 Jian-Bin Liu Zhong He Ke Jin Cong Ma Jun Xiang Jian-Hua Xiao Wei-Jian Chen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第25期3997-4004,共8页
AIM: To investigate dynamical and image pathological characteristics of the liver on magnetic resonance (MR) diffusion-weighted imaging (DWI) in the rabbit VX-2 tumor model. METHODS: Forty New Zealand rabbits we... AIM: To investigate dynamical and image pathological characteristics of the liver on magnetic resonance (MR) diffusion-weighted imaging (DWI) in the rabbit VX-2 tumor model. METHODS: Forty New Zealand rabbits were included in the study and VX-2 tumor piece was implanted intrahepatically. Fifteen animals received two intrahepatic implantations while 25 had one intrahepatical implantation. DWI, T1- and T2-weighted of magnetic resonance imaging (MRI) were carried out on the 7th and the 14th d after implantation and DWI was conducted, respectively on the 21th d. Ten VX-2 tumor samples were studied pathologically. RESULTS: The rate of lump detected by DWI, TlWI and T2WI was 78.7%, 10.7% and 53.5% (X^2 =32.61, P 〈 0.001) on the 7th d after implantation and 95.8%, 54.3% and 82.9% (X^2 = 21.50, P 〈 0.001) on the 14th d. The signal of most VX-2 tumors on DWI was uniform and it was equal diffusion coefficient (ADC). did not decrease on the 7th on the map of apparent The signal of VX tumors d after implantation, most of them slowly growing during the week following implantation without significant cell dying within the tumor. VX-2 tumors grew increasingly within 14 d after implantation but the signal of most VX-2 tumors on DWI or on the map of ADC was uniform or uneven and ADC of VX tumors decreased obscurely or slightly because tumor necrosis was still not obvious. On the 21th d after implantation, the signal of most VX-2 tumors on DWI or on the map of ADC was uneven because tumor necrosis was evident and ADC of VX-2 tumor necrotic areas decreased. The areas of viable cells in VX-2 tumors manifested a high signal on DWI and a low signal on the map of ADC. The areas of dead cells or necrosis in VX-2 tumors manifested low signals on DWI and low, equal or high signals on the map of ADC but they manifested high signals on DWI and on the map of ADC at the same time when the areas of necrotic tumor became liquefied or cystic. The border of tumors on DWI appeared gradually distinct and internal signals of tumor became progressively uneven. CONCLUSION: The manifestations of viable, necrotic and liquefied or cystic areas in VX-2 tumors on DWI are typical and DWI is of significant and potential values in clinical application in both the early detection and diagnosis of liver tumors. 展开更多
关键词 LIVER vx-2 tumor Diffusion-weightedimaging Apparent Diffusion Coefficient RABBIT
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Gene expression and MR diffusion-weighted imaging after chemoembolization in rabbit liver VX-2 tumor model 被引量:5
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作者 You-Hong Yuan En-Hua Xiao +6 位作者 Jian-Bin Liu Zhong He Ke Jin Cong Ma Jun Xiang Jian-Hua Xiao Wei-Jian Chen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第36期5557-5563,共7页
AIM: To investigate the dynamic characteristics and the correlation between PCNA, Bax, nm23, E-cadherin expression and apparent diffusion coefficient (ADC) on MR diffusion-weighted imaging (DWI) after chemoembolizatio... AIM: To investigate the dynamic characteristics and the correlation between PCNA, Bax, nm23, E-cadherin expression and apparent diffusion coefficient (ADC) on MR diffusion-weighted imaging (DWI) after chemoembolization in rabbit liver VX-2 tumor model. METHODS: Forty New Zealand rabbit liver VX-2 tumor models were included in the study. DWI was carried out periodically after chemoembolization. All VX-2 tumor samples in each group were examined by histopathology and Strept Avidin-Biotin Complex (SABC) immunohistochemical staining. RESULTS: The PCNA expression index in VX-2 tumors was higher than in the normal parenchyma around the tumor (P < 0.001). Nm23, Bax or E-caderin expression index in VX-2 tumors were lower than in the normal parenchyma around the tumor (all P < 0.001). PCNAand nm23 expression in the VX-2 tumor periphery first increased and then decreased (P < 0.001 and P = 0.03, respectively), while the expression of Bax and E-cadherin before and after chemoembolization was insignificant. When b-value was 100 s/mm2, there was a linear correlation between PCNA expression and ADC in the area of VX-2 tumor periphery (P < 0.001), and PCNA expression in VX-2 tumor periphery influenced the ADC. CONCLUSION: The potential of VX-2 tumor infiltrating and metastasizing decreases, while its ability to proliferate increases for a short time after chemoembolization. To some degree, the ADC value indirectly reflects the proliferation of VX-2 tumor cells. 展开更多
关键词 Rabbit liver vx-2 tumor CHEMOEMBOLIZATION Diffusion-weighted imaging Gene expression
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MR diffusion-weighted imaging of rabbit liver VX-2 tumor 被引量:3
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作者 You-HongYuan En-HuaXiao JunXiang Ke-LiTang KeJin Shi-JianYi QiangYin Rong-HuaYan ZhongHe Quan-LiangShang Wei-ZhouHu Su-WenYuan 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第20期3070-3074,共5页
AIM: To investigate the implanting method of rabbit liver VX-2 tumor and its MR diffusion-weighted imaging (DWI) characteristics. METHODS: Thirty-five New Zealand rabbits were included in the study. VX-2 tumor was imp... AIM: To investigate the implanting method of rabbit liver VX-2 tumor and its MR diffusion-weighted imaging (DWI) characteristics. METHODS: Thirty-five New Zealand rabbits were included in the study. VX-2 tumor was implanted subcutaneously in 14 rabbits and intrahepatically in 6 for pre-experiments. VX-2 tumor was implanted intrahepatically in 12 rabbits for experiment and three were used as the control group. DWI, T1- and T2-weighted of MRI were performed periodically in 15 rabbits for experiment before and after implantation. The distinction of VX-2 tumors on DWI was assessed by their apparent diffusion coefficient (ADC) values. The statistical significance was calculated by analysis of variance (ANOVA) of the randomized block design using SPSS10.0 software. RESULTS: The successful rate of subcutaneous implantation of VX-2 tumor was 29% (4/14) while that of intrahepatic implantation of it was 33% (2/6) in the preexperiment. The successful rate of intrahepatic implantation of VX-2 tumor in the experiment was 83% (10/12) and 15 tumors grew in 10 successfully implanted rabbits. The DWI signal of VX-2 tumor was high and became lower when the b value increased step by step. The signal of VX-2 tumor on the map of ADC was low. When the b value was 100 or 300 s/mm2, the ADC value of normal group and VX-2 tumor group was respectively 2.57±0.26, 1.73±0.31, 1.87±0.25 and 1.57±0.23 mm2/s. Their distinction was significant (F= 43.26, P<0.01), the tumor ADC value between b values 100 and 300 s/mm2 was significant (Tukey HSP,P<0.05) and the ADC value between VX-2 tumor and normal liver was also significant (Tukey HSP, P<0.01). VX-2 tumor developed quickly and metastasized early to all body, especially to the lung, liver, lymph nodes of mediastinum, etc. CONCLUSION: The DWI signal of rabbit VX-2 tumor has its characteristics on MR DWI and DWI plays an important role in diagnosing and discovering VX-2 tumor. 展开更多
关键词 Magnetic resonance imaging DIFFUSION-WEIGHTED LIVER vx-2 tumor RABBITS
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Calculus bovis inhibits M2 tumor-associated macrophage polarization via Wnt/β-catenin pathway modulation to suppress liver cancer 被引量:6
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作者 Zhen Huang Fan-Ying Meng +12 位作者 Lin-Zhu Lu Qian-Qian Guo Chang-Jun Lv Nian-Hua Tan Zhe Deng Jun-Yi Chen Zi-Shu Zhang Bo Zou Hong-Ping Long Qing Zhou Sha Tian Si Mei Xue-Fei Tian 《World Journal of Gastroenterology》 SCIE CAS 2024年第29期3511-3533,共23页
BACKGROUND Calculus bovis(CB),used in traditional Chinese medicine,exhibits anti-tumor effects in various cancer models.It also constitutes an integral component of a compound formulation known as Pien Tze Huang,which... BACKGROUND Calculus bovis(CB),used in traditional Chinese medicine,exhibits anti-tumor effects in various cancer models.It also constitutes an integral component of a compound formulation known as Pien Tze Huang,which is indicated for the treatment of liver cancer.However,its impact on the liver cancer tumor microenvironment,particularly on tumor-associated macrophages(TAMs),is not well understood.AIM To elucidate the anti-liver cancer effect of CB by inhibiting M2-TAM polarization via Wnt/β-catenin pathway modulation.METHODS This study identified the active components of CB using UPLC-Q-TOF-MS,evaluated its anti-neoplastic effects in a nude mouse model,and elucidated the underlying mechanisms via network pharmacology,transcriptomics,and molecular docking.In vitro assays were used to investigate the effects of CB-containing serum on HepG2 cells and M2-TAMs,and Wnt pathway modulation was validated by real-time reverse transcriptase-polymerase chain reaction and Western blot analysis.RESULTS This study identified 22 active components in CB,11 of which were detected in the bloodstream.Preclinical investigations have demonstrated the ability of CB to effectively inhibit liver tumor growth.An integrated approach employing network pharmacology,transcriptomics,and molecular docking implicated the Wnt signaling pathway as a target of the antineoplastic activity of CB by suppressing M2-TAM polarization.In vitro and in vivo experiments further confirmed that CB significantly hinders M2-TAM polarization and suppresses Wnt/β-catenin pathway activation.The inhibitory effect of CB on M2-TAMs was reversed when treated with the Wnt agonist SKL2001,confirming its pathway specificity.CONCLUSION This study demonstrated that CB mediates inhibition of M2-TAM polarization through the Wnt/β-catenin pathway,contributing to the suppression of liver cancer growth. 展开更多
关键词 Calculus bovis M2 tumor-associated macrophage polarization Liver cancer Wnt/β-catenin pathway tumor microenvironment
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Ferroptosis biomarkers predict tumor mutation burden's impact on prognosis in HER2-positive breast cancer 被引量:1
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作者 Jin-Yu Shi Xin Che +7 位作者 Rui Wen Si-Jia Hou Yu-Jia Xi Yi-Qian Feng Ling-Xiao Wang Shi-Jia Liu Wen-Hao Lv Ya-Fen Zhang 《World Journal of Clinical Oncology》 2024年第3期391-410,共20页
BACKGROUND Ferroptosis has recently been associated with multiple degenerative diseases.Ferroptosis induction in cancer cells is a feasible method for treating neoplastic diseases.However,the association of iron proli... BACKGROUND Ferroptosis has recently been associated with multiple degenerative diseases.Ferroptosis induction in cancer cells is a feasible method for treating neoplastic diseases.However,the association of iron proliferation-related genes with prognosis in HER2+breast cancer(BC)patients is unclear.AIM To identify and evaluate fresh ferroptosis-related biomarkers for HER2+BC.METHODS First,we obtained the mRNA expression profiles and clinical information of HER2+BC patients from the TCGA and METABRIC public databases.A four gene prediction model comprising PROM2,SLC7A11,FANCD2,and FH was subsequently developed in the TCGA cohort and confirmed in the METABRIC cohort.Patients were stratified into high-risk and low-risk groups based on their median risk score,an independent predictor of overall survival(OS).Based on these findings,immune infiltration,mutations,and medication sensitivity were analyzed in various risk groupings.Additionally,we assessed patient prognosis by combining the tumor mutation burden(TMB)with risk score.Finally,we evaluated the expression of critical genes by analyzing single-cell RNA sequencing(scRNA-seq)data from malignant vs normal epithelial cells.RESULTS We found that the higher the risk score was,the worse the prognosis was(P<0.05).We also found that the immune cell infiltration,mutation,and drug sensitivity were different between the different risk groups.The highrisk subgroup was associated with lower immune scores and high TMB.Moreover,we found that the combination of the TMB and risk score could stratify patients into three groups with distinct prognoses.HRisk-HTMB patients had the worst prognosis,whereas LRisk-LTMB patients had the best prognosis(P<0.0001).Analysis of the scRNAseq data showed that PROM2,SLC7A11,and FANCD2 were significantly differentially expressed,whereas FH was not,suggesting that these genes are expressed mainly in cancer epithelial cells(P<0.01).CONCLUSION Our model helps guide the prognosis of HER2+breast cancer patients,and its combination with the TMB can aid in more accurate assessment of patient prognosis and provide new ideas for further diagnosis and treatment. 展开更多
关键词 HER2+breast cancer Ferroptosis tumor mutation burden Single-cell RNA sequencing PROGNOSIS
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Correlation of tumor-associated macrophage density and proportion of M2 subtypes with the pathological stage of colorectal cancer
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作者 Fouzia Fazal Muhammad Arsalan Khan +2 位作者 Sumayya Shawana Rahma Rashid Muhammed Mubarak 《World Journal of Gastrointestinal Oncology》 SCIE 2024年第5期1878-1889,共12页
BACKGROUND Colorectal cancer(CRC)is a prevalent global malignancy with complex prognostic factors.Tumor-associated macrophages(TAMs)have shown paradoxical associations with CRC survival,particularly concerning the M2 ... BACKGROUND Colorectal cancer(CRC)is a prevalent global malignancy with complex prognostic factors.Tumor-associated macrophages(TAMs)have shown paradoxical associations with CRC survival,particularly concerning the M2 subset.AIM We aimed to establish a simplified protocol for quantifying M2-like TAMs and explore their correlation with clinicopathological factors.METHODS A cross-sectional study included histopathological assessment of paraffinembedded tissue blocks obtained from 43 CRC patients.Using CD68 and CD163 immunohistochemistry,we quantified TAMs in tumor stroma and front,focusing on M2 proportion.Demographic,histopathological,and clinical parameters were collected.RESULTS TAM density was significantly higher at the tumor front,with the M2 proportion three times greater in both zones.The tumor front had a higher M2 proportion,which correlated significantly with advanced tumor stage(P=0.04),pathological nodal involvement(P=0.04),and lymphovascular invasion(LVI,P=0.01).However,no significant association was found between the M2 proportion in the tumor stroma and clinicopathological factors.CONCLUSION Our study introduces a simplified protocol for quantifying M2-like TAMs in CRC tissue samples.We demonstrated a significant correlation between an increased M2 proportion at the tumor front and advanced tumor stage,nodal involvement,and LVI.This suggests that M2-like TAMs might serve as potential indicators of disease progression in CRC,warranting further investigation and potential clinical application. 展开更多
关键词 Colorectal cancer MACROPHAGES tumor stroma M2 subset tumor front tumor stage Lymphovascular invasion Prognosis tumor-associated macrophages Immunohistochemistry
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Insulin-like growth factor 2 targets IGF1R signaling transduction to facilitate metastasis and imatinib resistance in gastrointestinal stromal tumors
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作者 De-Gang Li Jia-Peng Jiang +4 位作者 Fan-Ye Chen Wei Wu Jun Fu Gong-He Wang Yu-Bo Li 《World Journal of Gastrointestinal Oncology》 SCIE 2024年第8期3585-3599,共15页
BACKGROUND Gastrointestinal stromal tumors(GISTs)are typical gastrointestinal tract neoplasms.Imatinib is the first-line therapy for GIST patients.Drug resistance limits the long-term effectiveness of imatinib.The reg... BACKGROUND Gastrointestinal stromal tumors(GISTs)are typical gastrointestinal tract neoplasms.Imatinib is the first-line therapy for GIST patients.Drug resistance limits the long-term effectiveness of imatinib.The regulatory effect of insulin-like growth factor 2(IGF2)has been confirmed in various cancers and is related to resistance to chemotherapy and a worse prognosis.AIM To further investigate the mechanism of IGF2 specific to GISTs.METHODS IGF2 was screened and analyzed using Gene Expression Omnibus(GEO:GSE225819)data.After IGF2 knockdown or overexpression by transfection,the phenotypes(proliferation,migration,invasion,apoptosis)of GIST cells were characterized by cell counting kit 8,Transwell,and flow cytometry assays.We used western blotting to evaluate pathway-associated and epithelial-mesenchymal transition(EMT)-associated proteins.We injected transfected cells into nude mice to establish a tumor xenograft model and observed the occurrence and metastasis of GIST.RESULTS Data from the GEO indicated that IGF2 expression is high in GISTs,associated with liver metastasis,and closely related to drug resistance.GIST cells with high expression of IGF2 had increased proliferation and migration,invasiveness and EMT.Knockdown of IGF2 significantly inhibited those activities.In addition,OEIGF2 promoted GIST metastasis in vivo in nude mice.IGF2 activated IGF1R signaling in GIST cells,and IGF2/IGF1R-mediated glycolysis was required for GIST with liver metastasis.GIST cells with IGF2 knockdown were sensitive to imatinib treatment when IGF2 overexpression significantly raised imatinib resistance.Moreover,2-deoxy-D-glucose(a glycolysis inhibitor)treatment reversed IGF2 overexpressionmediated imatinib resistance in GISTs.CONCLUSION IGF2 targeting of IGF1R signaling inhibited metastasis and decreased imatinib resistance by driving glycolysis in GISTs. 展开更多
关键词 Insulin-like growth factor 2 Gastrointestinal stromal tumors IGF1R GLYCOLYSIS Imatinib resistance
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Inhibition of M2 tumor-associated macrophages polarization by modulating the Wnt/β-catenin pathway as a possible liver cancer therapy method
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作者 Vladislav V Tsukanov Julia L Tonkikh +1 位作者 Edward V Kasparov Alexander V Vasyutin 《World Journal of Gastroenterology》 SCIE CAS 2024年第40期4399-4403,共5页
The problem of liver cancer is becoming increasingly important due to the epi-demic of metabolic diseases and persistent high alcohol consumption.This deter-mines great attention to the development and improvement of ... The problem of liver cancer is becoming increasingly important due to the epi-demic of metabolic diseases and persistent high alcohol consumption.This deter-mines great attention to the development and improvement of methods for early diagnosis and treatment of liver cancer.Huang et al presented a study in the World Journal of Gastroenterology,in which they showed that the use of the traditional Chinese medicine Calculus bovis(CB)can suppress tumor growth in mice by inhibiting M2 tumor-associated macrophages(TAM)through modulating the activity of the Wnt/β-catenin pathway.The interaction of CB components with the Wnt/β-catenin pathway,M2 TAM polarization,and tumor dynamics were studied using network pharmacology,transcriptomics,and molecular docking.It is now generally accepted that the polarization of TAM and the differentiation of the functions of M1 and M2 phagocytes are of great importance for the progression of neoplasms.It is assumed that M2 TAM promote proliferation and migration of tumor cells.Attempts to medicinally influence the Wnt/β-catenin pathway in order to modulate phagocyte polarization now belong to one of the most promising areas of immunotherapy of oncological diseases.Undoubtedly,the work of the Chinese authors deserves attention and further development. 展开更多
关键词 Liver cancer Treatment Calculus bovis tumor-associated macrophages M2 tumor Macrophage polarization Wnt/β-catenin pathway
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New 4-imino-4H-Chromeno[2,3-d]Pyrimidin-3(5H)-Amine: Synthesis, Cytotoxic Effects on Tumoral Cell Lines and in Silico ADMET Properties
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作者 Marwa Dhiabi Sirine Karoui +7 位作者 Mehdi Fakhfakh Souhir Abid Emmanuelle Limanton Rémy Le Guével Thierry Charlier Ludovic Paquin Jean-Pierre Bazureau Houcine Ammar 《International Journal of Organic Chemistry》 2024年第3期107-122,共16页
The synthesis of new 4-imino-4H-chromeno[2,3-d]pyrimidin-3(5H)-amine in four steps including one step under microwave dielectric heating is reported. The structural identity of the synthesized compounds was establishe... The synthesis of new 4-imino-4H-chromeno[2,3-d]pyrimidin-3(5H)-amine in four steps including one step under microwave dielectric heating is reported. The structural identity of the synthesized compounds was established according to their spectroscopic analysis, such as FT-IR, NMR and mass spectroscopy. These new compounds were tested for their antiproliferative activities on seven representative human tumoral cell lines (Huh7 D12, Caco2, MDA-MB231, MDA-MB468, HCT116, PC3 and MCF7) and also on fibroblasts. Among them, only the compounds 6c showed micromolar cytotoxic activity on tumor cell lines (1.8 50 50 > 25 μM). Finally, in silico ADMET studies ware performed to investigate the possibility of using of the identified compound 6c as potential anti-tumor compound. 展开更多
关键词 2-Amino-4H-Chromene 4H-Chromeno[2 3-d]Pyrimidin-3(5H)-Amine Microwave Irradiation tumoral Cell Line in Silico ADMET
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Serum tumor markers:Can they clinically implicate in type 2 diabetes mellitus?
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作者 Kotha Sugunakar Reddy Ilakkiya Priya Pandiaraj +1 位作者 Archana Gaur Sakthivadivel Varatharajan 《World Journal of Diabetes》 SCIE 2024年第7期1648-1650,共3页
“Serum tumor markers expression(CA19-9,CA242,and CEA)and its clinical implications in type 2 diabetes mellitus”authored by Meng and Shi presents an observational case-control study investigating the correlation betw... “Serum tumor markers expression(CA19-9,CA242,and CEA)and its clinical implications in type 2 diabetes mellitus”authored by Meng and Shi presents an observational case-control study investigating the correlation between tumor markers and type 2 diabetes mellitus(T2DM).The study explores the diagnostic accuracy of tumor markers,particularly cancer antigen 19-9(CA19-9),CA242,and carcinoembryonic antigen,in poorly controlled T2DM patients with hemoglobin A1c levels exceeding 9%,employing receiver operating characteristic curve analysis.Though study offers valuable insights into the potential utility of tumor markers in clinical practice,caution is advised regarding routine tumor marker testing due to challenges such as limited availability and cost.Additionally,the study overlooks potential confounding factors like smoking and alcohol consumption.Variations in CA19-9 and CA242 expression underscore the complex interplay between tumor markers and systemic diseases,warranting further investigation into their diagnostic and prognostic implications.While Meng and Shi represent a significant contribution to the field,more extensive research is needed to fully elucidate the role of tumor markers in diabetes management and beyond. 展开更多
关键词 Cancer antigen 19-9 Cancer antigen 242 Carcinoembryonic antigen tumor markers Type 2 diabetes mellitus
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清燥救肺汤联合痰热清注射液防治放射性肺损伤的临床疗效及对免疫指标、IL-2和TNF-α表达的影响 被引量:3
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作者 王静 孙旭 +2 位作者 易宣洪 汪萍 王志武 《中医肿瘤学杂志》 2024年第1期43-48,共6页
目的探讨清燥救肺汤联合痰热清注射液防治放射性肺损伤的临床疗效及对免疫指标、白细胞介素-2(interleukin-2,IL-2)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)表达的影响。方法选择2020年9月~2022年12月在唐山市人民医院放化... 目的探讨清燥救肺汤联合痰热清注射液防治放射性肺损伤的临床疗效及对免疫指标、白细胞介素-2(interleukin-2,IL-2)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)表达的影响。方法选择2020年9月~2022年12月在唐山市人民医院放化科接受胸部三维适形放射治疗的肺癌患者100例,随机分为对照组和治疗组各50例。对照组采用单纯放疗,治疗组在对照组基础上给予清燥救肺汤联合痰热清注射液。放疗结束后3个月比较两组近期疗效、放射性肺炎和不良反应发生情况,及放疗前与放疗结束后3个月生存质量、免疫指标、IL-2和TNF-α水平变化。结果治疗组近期有效率高于对照组(P<0.05)。2组放疗结束后3个月卡氏功能状态(karnofsky performance status,KPS)评分高于放疗前(P<0.05);治疗组放疗结束后3个月KPS评分改善情况优于对照组(P<0.05)。2组放疗结束后3个月中医证候积分均低于放疗前(P<0.05);治疗组放疗结束后3个月中医证候积分改善优于对照组(P<0.05)。治疗组患者放疗结束后3个月CD3+、CD4+和CD4+/CD8+高于放疗前(P<0.05),改善情况优于对照组(P<0.05),对照组患者放疗结束后3个月CD3+、CD4+/CD8+较放疗前无明显变化(P>0.05)。2组患者放疗结束后3个月血清IL-2水平高于放疗前,而TNF-α水平低于放疗前(P<0.05);治疗组患者放疗结束后3个月血清IL-2水平和TNF-α水平改善情况均优于对照组(P<0.05)。2组不良反应发生情况比较差异无统计学意义(P>0.05)。结论清燥救肺汤联合痰热清注射液可明显降低放射性肺炎发生率,改善部分免疫指标水平,减轻细胞炎性反应。 展开更多
关键词 清燥救肺汤 痰热清注射液 放射性肺炎 免疫功能 白细胞介素-2 肿瘤坏死因子-α
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SHP-2在肿瘤相关巨噬细胞中的研究进展
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作者 武雪亮 樊建春 +7 位作者 郭飞 张琦 薛军 王西墨 孙光源 刘建玲 韩磊 高树全 《中国比较医学杂志》 CAS 北大核心 2024年第1期171-176,共6页
肿瘤相关巨噬细胞(TAMs)是肿瘤免疫微环境(TIME)中的优势细胞群,是TIME中免疫系统抑制和肿瘤细胞增殖最重要的调节细胞。Src同源2蛋白酪氨酸磷酸酶2(SHP-2)是一种非受体蛋白酪氨酸磷酸酶,该磷酸酶在从细胞表面到细胞核的信号传递中发挥... 肿瘤相关巨噬细胞(TAMs)是肿瘤免疫微环境(TIME)中的优势细胞群,是TIME中免疫系统抑制和肿瘤细胞增殖最重要的调节细胞。Src同源2蛋白酪氨酸磷酸酶2(SHP-2)是一种非受体蛋白酪氨酸磷酸酶,该磷酸酶在从细胞表面到细胞核的信号传递中发挥重要作用,且是介导细胞增殖和分化的关键细胞内调节因子,参与多种生长因子和细胞因子的信号通路。最近的研究表明,SHP-2是决定TAMs功能的一个关键酶,但是由于其功能多变,在不同的实体瘤微环境中发挥不同甚至是相反的作用。基于此,本文综述了SHP-2在TAMs功能及在相关实体瘤中的作用,为肿瘤的免疫和靶向治疗提供坚实的科学依据。 展开更多
关键词 蛋白酪氨酸磷酸酶2 肿瘤相关巨噬细胞 临床研究 作用机制
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FGL2在胃肠间质瘤的表达及临床意义
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作者 张向莲 王兴芬 梁锐 《中国免疫学杂志》 CAS CSCD 北大核心 2024年第4期752-755,共4页
目的:探讨纤维蛋白原样蛋白2(FGL2)在胃肠道间质瘤(GIST)中的表达及临床意义。方法:运用免疫组化EnVision二步法检测158例GIST患者中FGL2蛋白的表达情况,分析其与临床病理参数之间的相关性,并探讨FGL2蛋白与GIST中肿瘤浸润性淋巴细胞(TI... 目的:探讨纤维蛋白原样蛋白2(FGL2)在胃肠道间质瘤(GIST)中的表达及临床意义。方法:运用免疫组化EnVision二步法检测158例GIST患者中FGL2蛋白的表达情况,分析其与临床病理参数之间的相关性,并探讨FGL2蛋白与GIST中肿瘤浸润性淋巴细胞(TILs)CD3^(+)T、CD8^(+)T、CD20^(+)T及CD68^(+)T细胞计数之间的关系。结果:FGL2蛋白在GIST中的表达与肿瘤大小、NIH危险度分级、坏死及核分裂象计数呈负相关(P<0.05),与患者性别及肿瘤发生部位差异无统计学意义(P>0.05)。在GIST中,FGL2表达与低CD3^(+)T细胞计数相关(P<0.05),而与CD8^(+)T、CD20^(+)T及CD68^(+)T细胞计数差异无统计学意义(P>0.05)。结论:FGL2蛋白参与GIST的发生和发展,其表达水平在一定程度上可预测GIST的恶性潜能。FGL2蛋白可能通过调节肿瘤微环境参与GIST的发生和发展。 展开更多
关键词 纤维蛋白原样蛋白2 胃肠道间质瘤 肿瘤浸润相关淋巴细胞
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TPL2抑制剂对溃疡性结肠炎小鼠的作用及机制研究
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作者 杨洁 刘洁 +3 位作者 陈吉 段聿 崔琴 赵翠娟 《胃肠病学和肝病学杂志》 CAS 2024年第6期680-684,共5页
目的探讨抑制肿瘤进展位点2(tumor progression locus 2,TPL2)/细胞外信号调节激酶1/2(extracellular signal-regulated kinase1/2,ERK1/2)信号通路对实验性溃疡性结肠炎(ulcerative colitis,UC)小鼠肠道损伤和炎症的保护作用。方法将30... 目的探讨抑制肿瘤进展位点2(tumor progression locus 2,TPL2)/细胞外信号调节激酶1/2(extracellular signal-regulated kinase1/2,ERK1/2)信号通路对实验性溃疡性结肠炎(ulcerative colitis,UC)小鼠肠道损伤和炎症的保护作用。方法将30只C57BL/6J小鼠分为正常对照组、模型对照组、高剂量TPL2抑制剂组、低剂量TPL2抑制剂组、美沙拉嗪组,每组6只。比较各组小鼠疾病活动指数(disease activity index,DAI)和组织学损伤评估(histological index,HI)评分,采用实时定量PCR检测结肠中IL-6、TNF-α、TPL2和ERK1/2 mRNA表达水平,Western blotting检测结肠中TPL2、ERK1/2蛋白表达水平。结果与正常对照组相比较,模型对照组小鼠的DAI、HI评分均升高,结肠中IL-6、TNF-α、TPL2、ERK1/2 mRNA和蛋白表达均增加(均P<0.05)。与模型对照组相比较,给予TPL2抑制剂干预的两组小鼠DAI、HI评分均降低,结肠中IL-6、TNF-αmRNA相对表达量降低,结肠中TPL2、ERK1/2 mRNA和蛋白表达均下降(均P<0.05)。结论TPL2抑制剂可以改善UC小鼠肠道组织损伤与炎症,其分子机制与抑制其下游ERK1/2信号通路相关。 展开更多
关键词 溃疡性结肠炎 抑制肿瘤进展位点2 细胞外信号调节激酶 炎症因子
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外周血sIL-2R、CD4^(+)/CD8^(+)、TNF-α对初治活动性肺结核老年患者化疗疗效的评估价值
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作者 刘会 高江彦 +10 位作者 霍琳 张晓光 张会晓 张焕 付洪义 王显雷 安贺娟 王勇 刘锐 陈素丽 李卫红 《国际检验医学杂志》 CAS 2024年第6期738-743,750,共7页
目的探讨外周血可溶性白细胞介素2受体(sIL-2R)、CD4^(+)淋巴细胞百分比/CD8^(+)淋巴细胞百分比比值(下称CD4^(+)/CD8^(+))、肿瘤坏死因子-α(TNF-α)对初治活动性肺结核老年患者化疗疗效的评估价值。方法将2019年12月至2022年12月该院... 目的探讨外周血可溶性白细胞介素2受体(sIL-2R)、CD4^(+)淋巴细胞百分比/CD8^(+)淋巴细胞百分比比值(下称CD4^(+)/CD8^(+))、肿瘤坏死因子-α(TNF-α)对初治活动性肺结核老年患者化疗疗效的评估价值。方法将2019年12月至2022年12月该院收治的102例初治活动性肺结核老年患者纳入研究作为观察组,另选取102例年龄≥60岁且同期于该院体检的健康者作为对照组。比较两组外周血sIL-2R、TNF-α、CD4^(+)/CD8^(+)水平并分析sIL-2R、TNF-α、CD4^(+)/CD8^(+)间的相关性。观察组均采用2HRZE/4HR抗结核治疗方案,比较观察组治疗前、治疗1个月、治疗6个月时不同疗效患者外周血sIL-2R、TNF-α、CD4^(+)/CD8^(+);分析sIL-2R、CD4^(+)/CD8^(+)、TNF-α水平与疗效的相关性;采用受试者工作特征(ROC)曲线分析各指标用于老年患者化疗疗效评估的效能。结果观察组sIL-2R、TNF-α水平高于对照组,而CD4^(+)/CD8^(+)低于对照组,差异均有统计学意义(P<0.05)。观察组sIL-2R、TNF-α与CD4^(+)/CD8^(+)呈负相关(P<0.05),sIL-2R与TNF-α呈正相关(P<0.05)。治疗1个月、治疗6个月时显效患者sIL-2R、TNF-α水平低于有效患者,而后者又低于无效患者,显效患者CD4^(+)/CD8^(+)高于有效患者,而后者又高于无效患者,差异均有统计学意义(P<0.05)。sIL-2R、TNF-α水平与疗效呈负相关(P<0.05),CD4^(+)/CD8^(+)与疗效呈正相关(P<0.05)。ROC曲线分析显示,治疗1个月、6个月时sIL-2R、CD4^(+)/CD8^(+)、TNF-α联合用于评估疗效的曲线下面积(AUC)明显大于各时间点单项指标用于评估的AUC(P<0.05),而且治疗6个月时各指标联合评估的AUC大于治疗1个月(P<0.05)。结论初治活动性肺结核老年患者sIL-2R、CD4^(+)/CD8^(+)、TNF-α水平与患者疗效密切相关,将以上指标联合用于评估患者化疗疗效具有一定参考价值。 展开更多
关键词 可溶性白介素2受体 CD4 CD8 肿瘤坏死因子-α 活动性肺结核 化疗 老年患者
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外周血TLR2、TIPE2、NLR联合检测对机械通气新生儿呼吸机相关性肺炎的预测价值
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作者 曲海新 郭卫平 +2 位作者 袁二伟 历奇 刘璐 《检验医学与临床》 CAS 2024年第20期2985-2989,共5页
目的探讨外周血Toll样受体2(TLR2)、肿瘤坏死因子α诱导蛋白8样因子2(TIPE2)、中性粒细胞与淋巴细胞比值(NLR)联合检测对机械通气新生儿呼吸机相关性肺炎(VAP)的预测价值。方法选取2020年6月至2023年6月河北北方学院附属第一医院进行机... 目的探讨外周血Toll样受体2(TLR2)、肿瘤坏死因子α诱导蛋白8样因子2(TIPE2)、中性粒细胞与淋巴细胞比值(NLR)联合检测对机械通气新生儿呼吸机相关性肺炎(VAP)的预测价值。方法选取2020年6月至2023年6月河北北方学院附属第一医院进行机械通气治疗的232例新生儿作为研究对象。将发生VAP的新生儿纳入VAP组,其余纳入非VAP组。检测并比较两组外周血TLR2、TIPE2表达水平和NLR。绘制受试者工作特征(ROC)曲线评估外周血TLR2、TIPE2、NLR单独及联合预测机械通气新生儿VAP发生的价值。采用多因素Logistic回归分析机械通气新生儿发生VAP的影响因素。结果232例机械通气新生儿中,60例发生VAP,发生率为25.86%,纳入VAP组;其余172例未发生VAP,纳入非VAP组。VAP组和非VAP组机械通气时间、置管次数和吸痰次数比较,差异均有统计学意义(P<0.05)。VAP组TLR2相对表达水平和NLR高于非VAP组,TIPE2相对表达水平低于非VAP组,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,机械通气时间≥3 d、置管次数≥2次、吸痰次数≥5次/天、TLR2相对表达水平升高、NLR升高是械通气新生儿发生VAP的危险因素(P<0.05),TIPE2相对表达水平升高是新生儿发生VAP的保护因素(P<0.05)。ROC曲线分析结果显示,TLR2、TIPE2和NLR单独预测机械通气新生儿发生VAP的曲线下面积(AUC)分别为0.783、0.754、0.745;且外周血TLR2、TIPE2、NLR联合检测预测机械通气新生儿发生VAP的AUC为0.823。结论外周血TLR2、TIPE2和NLR均是机械通气新生儿发生VAP的独立影响因素,且联合检测有助于预测VAP的发生。 展开更多
关键词 机械通气 新生儿 呼吸机相关性肺炎 TOLL样受体2 肿瘤坏死因子α诱导蛋白8样因子2 中性粒细胞与淋巴细胞比值
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自然杀伤细胞2组成员A、程序性死亡因子配体1表达检测对PD-L1阻断免疫治疗肌层浸润性膀胱癌反应性的预测价值
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作者 赵刚刚 张鸿毅 +3 位作者 肖克兵 杨辉 李子峰 赵华才 《陕西医学杂志》 CAS 2024年第2期252-256,共5页
目的:探究自然杀伤细胞2组成员A(NKG2A)及程序性死亡因子配体1(PD-L1)表达检测对PD-L1阻断免疫治疗肌层浸润性膀胱癌反应性的预测价值。方法:选取100例肌层浸润性膀胱癌患者作为研究对象,对其行PD-L1阻断免疫治疗后,根据治疗反应性将其... 目的:探究自然杀伤细胞2组成员A(NKG2A)及程序性死亡因子配体1(PD-L1)表达检测对PD-L1阻断免疫治疗肌层浸润性膀胱癌反应性的预测价值。方法:选取100例肌层浸润性膀胱癌患者作为研究对象,对其行PD-L1阻断免疫治疗后,根据治疗反应性将其分为缓解组和未缓解组;对缓解组患者随访3个月,将其分为复发组和未复发组。比较两组患者NKG2A和PD-L1在CD4^(+)和CD8^(+)上的表达水平,采用ROC曲线分析NKG2A和PD-L1预测膀胱癌患者治疗反应性的价值。结果:100例研究对象中,缓解组患者72例(72.00%),未缓解组患者28例(28.00%),两组性别、年龄比较无统计学差异(均P>0.05),但缓解组患者肿瘤直径小于未缓解组,NKG2A、PD-L1/CD4^(+)和PD-L1/CD8^(+)表达水平均低于未缓解组(均P<0.05)。膀胱癌患者NKG2A、PD-L1/CD4^(+)和PD-L1/CD8^(+)表达预测免疫治疗后缓解的AUC值分别为0.771、0.724、0.710;联合诊断的AUC为0.836。72例缓解患者中,出现复发29例(40.28%),未出现复发43例(59.72%),两组性别、年龄以及肿瘤直径比较无统计学差异(均P>0.05),但复发组NKG2A、PD-L1/CD4^(+)和PD-L1/CD8^(+)表达水平均高于未复发组(均P<0.05)。NKG2A、PD-L1/CD4^(+)和PD-L1/CD8^(+)表达预测膀胱癌患者缓解后复发的AUC值分别为0.775、0.740、0.728;联合诊断的AUC为0.874。结论:NKG2A、PD-L1/CD4^(+)和PD-L1/CD8^(+)在不同治疗反应性肌层浸润性膀胱癌患者外周血中表达水平不同,三者对于膀胱癌患者PD-L1阻断免疫治疗反应性均有一定的预测价值,且三者联合预测效能最佳。 展开更多
关键词 膀胱癌 肿瘤直径 自然杀伤细胞2组成员A 程序性死亡因子配体1 免疫治疗 反应性
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低剂量CT结合SHOX2、RASSF1A甲基化在肺癌早期预警中的应用
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作者 李志娟 董红 +2 位作者 田涛 于哲 李晓敏 《中国CT和MRI杂志》 2024年第2期73-76,共4页
目的探讨低剂量CT结合Ras相关区域家族蛋白1A(RASSF1A)、矮小同源盒基因2(SHOX2)甲基化在肺癌早期预测中的应用价值。方法选取2021年1月~2023年1月我院90例拟行肺结节手术患者,根据手术病理学分为肺良性结节组和肺癌组。2组均于术前行... 目的探讨低剂量CT结合Ras相关区域家族蛋白1A(RASSF1A)、矮小同源盒基因2(SHOX2)甲基化在肺癌早期预测中的应用价值。方法选取2021年1月~2023年1月我院90例拟行肺结节手术患者,根据手术病理学分为肺良性结节组和肺癌组。2组均于术前行低剂量CT检查、SHOX2、RASSF1A甲基化检测,采用Kappa指数分析上述检查结果与手术病理学一致性,分析低剂量CT、SHOX2、RASSF1A甲基化与血清肿瘤标志物[癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)、鳞状细胞癌抗原(SCC-Ag)、细胞角蛋白19片段(CYFRA21)]对肺癌诊断效能,采用Spearman低剂量CT检查、SHOX2、RASSF1A甲基化与临床病理特征相关性。结果低剂量CT、SHOX2、RASSF1甲基化及三者联合分别确定40例、43例、46例、58例肺癌,三者联合与手术病理学诊断肺癌效能一致性Kappa值为0.951;三者联合诊断肺癌敏感度96.67%、准确度97.78%均高于三者单一诊断效能(P<0.05);肺癌患者血清CEA、SCC、NSE、CYFRA21水平均高于肺良性结节患者(P<0.05);低剂量CT联合SHOX2、RASSF1甲基化诊断肺癌效能的AUC为0.983,近似于四种血清肿瘤标志物诊断肺癌效能的AUC 0.933;不同肿瘤直径、临床分期、组织学分化肺癌患者低剂量CT检出率及SHOX2、RASSF1A甲基化阳性率比较差异有统计学意义(P<0.05);肺癌患者低剂量CT检出率、SHOX2及RASSF1A甲基化阳性率与肿瘤直径、临床分期呈正相关,与组织学分化呈负相关(P<0.05)。结论低剂量CT联合SHOX2及RASSF1A甲基化可用于肺癌早期预警中,临床可通过其进行早期诊断、评估病情进展程度,以针对性展开后续治疗,改善预后。 展开更多
关键词 低剂量CT 矮小同源盒基因2 Ras相关区域家族蛋白1A 肺癌 血清肿瘤标志物
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双靶向新辅助药物联合不同化疗治疗HER-2阳性乳腺癌临床疗效比较
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作者 陈方红 高东 +2 位作者 张平宇 周杰 黄亮 《中国药业》 CAS 2024年第8期95-99,共5页
目的探讨双靶向新辅助药物联合不同化学药物治疗(简称化疗)的方案治疗人类表皮生长因子受体-2(HER-2)阳性乳腺癌的临床疗效。方法选取凉山彝族自治州第一人民医院2019年1月至2022年12月收治的HER-2阳性乳腺癌患者110例,按治疗方案的不... 目的探讨双靶向新辅助药物联合不同化学药物治疗(简称化疗)的方案治疗人类表皮生长因子受体-2(HER-2)阳性乳腺癌的临床疗效。方法选取凉山彝族自治州第一人民医院2019年1月至2022年12月收治的HER-2阳性乳腺癌患者110例,按治疗方案的不同分为TcbHP组和AC-THP组,各55例。TcbHP组患者予曲妥珠单抗和帕妥珠单抗联合紫杉类(多西他赛或紫杉醇)和卡铂治疗,以21 d为1个周期,共治疗6个周期;AC-THP组患者予曲妥珠单抗和帕妥珠单抗联合蒽环类药物(吡柔比星或表柔比星)和环磷酰胺治疗,以21 d为1个周期,曲妥珠单抗和帕妥珠单抗及其他药物分别治疗4个周期,共8个周期。结果TcbHP组患者病理完全缓解率为61.80%,稍高于AC-THP组的50.90%(P>0.05)。治疗后,TcbHP组恶心,呕吐,腹泻,心脏毒性及手足综合征程度均显著低于AC-THP组(P<0.05);各肿瘤标志物[糖类抗原(CA19-9,CA125,CA153),癌胚抗原(CEA)]水平均显著低于AC-THP组(P<0.05);左心室射血分数(LVEF)及血清心肌肌钙蛋白I(cTnI)、肌酸激酶同工酶MB(CK-MB)、氨基末端脑钠肽前体(NT-proBNP)水平均无明显变化(P>0.05),且均显著优于AC-THP组(P<0.05)。结论TcbHP方案治疗HER-2阳性乳腺癌的疗效与AC-THP方案相当,但前者降低肿瘤标志物生成的作用更明显,且心脏毒性风险相对更低。 展开更多
关键词 化学药物治疗 双靶向药物 新辅助治疗 人类表皮生长因子受体-2 乳腺癌 肿瘤标志物 心脏毒性
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