Objective: To investigate the effects of different concentrations of β-glucan on the repair of damaged vaginal mucosa, the expression of vascular endothelial growth factor (VEGF), and the inflammatory factor-6 (IL-6)...Objective: To investigate the effects of different concentrations of β-glucan on the repair of damaged vaginal mucosa, the expression of vascular endothelial growth factor (VEGF), and the inflammatory factor-6 (IL-6) in vaginal tissues. Methods: Thirty-six adult female specific pathogen free (SPF)-grade Wistar rats were randomly divided into 3 phase groups with 12 rats each. Vaginal inflammation rat models were established by injecting phenol gel into the vagina of each rat at a dose of 0.1 ml/100g body weight. After modeling, rats were divided into 4 groups based on different concentrations of the test agent. The control group was injected with 0.5 ml of saline, experimental group A was injected with 0.375 ml saline 0.125 ml β-glucan, experimental group B was injected with 0.25 ml saline 0.25 ml β-glucan, and experimental group C was injected with 0.50 ml β-glucan. The injection sites were selected at the 3 o’clock and 9 o’clock positions of the vagina. Rats were sacrificed at 7-, 14-, and 28-days post-injection, and tissue samples were collected from the injection sites and prepared for histological analysis. New blood vessels and fibroblast numbers in the tissues were observed after Hematoxylin-eosin (HE) staining. The expression levels of VEGF and IL-6 in the tissues were measured using quantificational reverse transcription polymerase chain reaction (qRT-PCR). Results: Histological examination of vaginal tissue specimens at 7-, 14-, and 28-days post-injection showed that on day 7, there were no significant changes in the experimental groups compared to the control group. However, on days 14 and 28, the experimental groups showed more new blood vessels, macrophages, and fibroblasts with increased activity compared to the control group. The expression levels of VEGF in vaginal tissues were elevated on days 14 and 28 in the experimental groups. The comparison of IL-6 levels in vaginal tissues on day 28 showed that serum IL-6 levels returned to normal, and there was no statistically significant difference between the experimental and control groups. Conclusion: In the 3 experimental phases, the increase in VEGF levels in vaginal tissues on day 14 post-injection was more pronounced with higher concentrations of β-glucan, and IL-6 levels returned to normal on day 28. β-Glucan can enhance VEGF levels in damaged vaginal tissues, promote the repair of damaged vaginal tissues, and higher concentrations of β-glucan have a better effect.展开更多
BACKGROUND With the increasing trend of vaginal birth after caesarean delivery(VBAC),evaluation of the feasibility and safety of a second VBAC with grand multiparity is worth considering.Intrapartum uterine rupture is...BACKGROUND With the increasing trend of vaginal birth after caesarean delivery(VBAC),evaluation of the feasibility and safety of a second VBAC with grand multiparity is worth considering.Intrapartum uterine rupture is diagnosed in approximately one-fifth of all VBAC cases following successful vaginal delivery.To our knowledge,no report is available on the application of laparoscopy to repair postpartum uterine rupture after a successful second VBAC in China.CASE SUMMARY A 31-year-old woman(gravida 5,para 2)at 39 wk and 5 d of gestation was admitted to the hospital in labour.After a successful VBAC and observation for approximately 13 h,the patient complained of progressive abdominal pain.Given the symptoms,signs,and auxiliary examination results,intraperitoneal bleeding was considered.Because the patient was stable and ultrasound imaging was the only method available to assess the possibility of rupture,we recommended laparoscopy to clarify the diagnosis and for prompt laparoscopic uterine repair or exploratory laparotomy if necessary.Operative findings included transverse uterine scar rupture at the lower uterine segment of approximately 5.0 cm in length and 800 mL of intraoperative pelvic haemoperitoneum.Finally,she successfully underwent laparoscopic repair of uterine rupture and recovered very well according to three-dimensional magnetic resonance imaging at 42 d postpartum.CONCLUSION Routine postpartum intrauterine exploration is not beneficial to the mother and may even increase the risk of rupture.This case highlights a laparoscopic approach for repairing uterine rupture in the immediate postpartum period.展开更多
Introduction: Vaginal adenosis is one of the rare diseases of the vagina, and almost all patients are asymptomatic. We report a case of spontaneous vaginal adenosis, which caused vesicovaginal fistula. Case Presentati...Introduction: Vaginal adenosis is one of the rare diseases of the vagina, and almost all patients are asymptomatic. We report a case of spontaneous vaginal adenosis, which caused vesicovaginal fistula. Case Presentation: Our patient was a 25-year-old Japanese woman. She was admitted to our hospital, and her chief complaint was continuous urine flow from the vagina. We found a tumor with a vesicovaginal fistula in her vagina. Subsequent cytological analysis of vaginal smears showed a normal vaginal mucosa. The patient underwent tumor resection and a fistula patch. Pathological diagnosis was adenosis of the vagina, even though the patient had no known history of intrauterine diethylstilbestrol exposure or Müllerian developmental abnormalities. Conclusion: The clinical course of our case was a malignant tumor, which invasively bored a hole in the vaginal wall forming a vesicovaginal fistula, even though it was a benign lesion. Therefore, overzealous treatment should be avoided in this case.展开更多
目的 分析老年子宫脱垂患者术后新发压力性尿失禁(de novo SUI)情况及其影响因素。方法 回顾性分析泗县人民医院2020年10月~2023年5月期间因子宫脱垂行手术治疗的60例患者,调查患者术后de novo SUI发生情况,将患者分为无de novo SUI组及...目的 分析老年子宫脱垂患者术后新发压力性尿失禁(de novo SUI)情况及其影响因素。方法 回顾性分析泗县人民医院2020年10月~2023年5月期间因子宫脱垂行手术治疗的60例患者,调查患者术后de novo SUI发生情况,将患者分为无de novo SUI组及de novo SUI组,通过单因素及多因素logistic回归分析影响患者术后de novo SUI发生的相关因素。结果 60例行手术治疗的子宫脱垂患者,18例患者术后发生de novo SUI,发病率为30.00%。单因素分析结果显示,体质量指数(BMI)、高血压史、巨大儿分娩史、糖尿病史、便秘史、盆腔手术史、雌激素治疗史是影响老年子宫脱垂患者术后de novo SUI的相关因素(χ^(2)=5.701、4.775、6.782、4.113、3.951、5.644、9.966,P<0.05)。多因素logistic分析结果显示,高血压史、巨大儿分娩史、糖尿病史、便秘史、盆腔手术史为老年子宫脱垂患者术后de novo SUI的独立危险因素(P<0.05)。结论 老年子宫脱垂患者术后存在一定的de novo SUI发生风险,高血压史、巨大儿分娩史、糖尿病史、便秘史、盆腔手术史为老年子宫脱垂患者术后de novo SUI的独立危险因素。展开更多
Background：Although repair augmented with mesh has been proved its priority in anatomical and functional recovery after anterior compartment reconstruction,the data about posterior compartment are scarce.The aim of t...Background：Although repair augmented with mesh has been proved its priority in anatomical and functional recovery after anterior compartment reconstruction,the data about posterior compartment are scarce.The aim of this study was to compare bowel functional outcome of posterior vaginal compartment repair with and without mesh in patients with pelvic organ prolapse （POP）.Methods：This was a prospective,double-blind,clinical pilot study of 22 postmenopausal women with symptomatic POP （overall POP-quantification [POP-Q] Stage Ⅲ-ⅣV） who underwent total pelvic floor reconstruction.Patients were grouped according to the use of mesh for posterior vaginal compartment repair：A mesh group and a nonmesh group.POP-Q stage,the pelvic floor impact questionnaire short form-7 （PFIQ-7） and anorectal manometry were evaluated before and 3 months after surgery.Anatomical success was defined as POP-Q Stage Ⅱ or less.A t-test was used to compare preoperative with postoperative data in the two groups.Results：Totally,17 （71％） were available for the follow-up.POP-Q measurements improved significantly compared to baseline （P ＜ 0.05） in both groups.No recurrence was observed.Subjects in both groups reported improvement in pelvic floor symptoms,and there was no significant difference in the PFIQ-7 score between groups at follow-up （P ＞ 0.05）.Compared with baseline,the nonmesh group exhibited a statistically significant decrease in anal residual pressure,a significant increase in the anorectal pressure difference during bowel movement,and a reduced rate ofdyssynergia defecation pattern （P ＜ 0.05）.Conclusions：Provided there is sufficient support for the anterior wall and apex of vagina with mesh,posterior compartment repair without mesh may be as effective as repair with mesh for anatomical recovery while providing better anorectal motor function.展开更多
文摘Objective: To investigate the effects of different concentrations of β-glucan on the repair of damaged vaginal mucosa, the expression of vascular endothelial growth factor (VEGF), and the inflammatory factor-6 (IL-6) in vaginal tissues. Methods: Thirty-six adult female specific pathogen free (SPF)-grade Wistar rats were randomly divided into 3 phase groups with 12 rats each. Vaginal inflammation rat models were established by injecting phenol gel into the vagina of each rat at a dose of 0.1 ml/100g body weight. After modeling, rats were divided into 4 groups based on different concentrations of the test agent. The control group was injected with 0.5 ml of saline, experimental group A was injected with 0.375 ml saline 0.125 ml β-glucan, experimental group B was injected with 0.25 ml saline 0.25 ml β-glucan, and experimental group C was injected with 0.50 ml β-glucan. The injection sites were selected at the 3 o’clock and 9 o’clock positions of the vagina. Rats were sacrificed at 7-, 14-, and 28-days post-injection, and tissue samples were collected from the injection sites and prepared for histological analysis. New blood vessels and fibroblast numbers in the tissues were observed after Hematoxylin-eosin (HE) staining. The expression levels of VEGF and IL-6 in the tissues were measured using quantificational reverse transcription polymerase chain reaction (qRT-PCR). Results: Histological examination of vaginal tissue specimens at 7-, 14-, and 28-days post-injection showed that on day 7, there were no significant changes in the experimental groups compared to the control group. However, on days 14 and 28, the experimental groups showed more new blood vessels, macrophages, and fibroblasts with increased activity compared to the control group. The expression levels of VEGF in vaginal tissues were elevated on days 14 and 28 in the experimental groups. The comparison of IL-6 levels in vaginal tissues on day 28 showed that serum IL-6 levels returned to normal, and there was no statistically significant difference between the experimental and control groups. Conclusion: In the 3 experimental phases, the increase in VEGF levels in vaginal tissues on day 14 post-injection was more pronounced with higher concentrations of β-glucan, and IL-6 levels returned to normal on day 28. β-Glucan can enhance VEGF levels in damaged vaginal tissues, promote the repair of damaged vaginal tissues, and higher concentrations of β-glucan have a better effect.
基金Supported by International Peace Maternal and Child Health Institute Clinical Research Program,No.YN201910Translational Medicine Cross Fund from Shanghai Jiao Tong University,No.YG2017QN38.
文摘BACKGROUND With the increasing trend of vaginal birth after caesarean delivery(VBAC),evaluation of the feasibility and safety of a second VBAC with grand multiparity is worth considering.Intrapartum uterine rupture is diagnosed in approximately one-fifth of all VBAC cases following successful vaginal delivery.To our knowledge,no report is available on the application of laparoscopy to repair postpartum uterine rupture after a successful second VBAC in China.CASE SUMMARY A 31-year-old woman(gravida 5,para 2)at 39 wk and 5 d of gestation was admitted to the hospital in labour.After a successful VBAC and observation for approximately 13 h,the patient complained of progressive abdominal pain.Given the symptoms,signs,and auxiliary examination results,intraperitoneal bleeding was considered.Because the patient was stable and ultrasound imaging was the only method available to assess the possibility of rupture,we recommended laparoscopy to clarify the diagnosis and for prompt laparoscopic uterine repair or exploratory laparotomy if necessary.Operative findings included transverse uterine scar rupture at the lower uterine segment of approximately 5.0 cm in length and 800 mL of intraoperative pelvic haemoperitoneum.Finally,she successfully underwent laparoscopic repair of uterine rupture and recovered very well according to three-dimensional magnetic resonance imaging at 42 d postpartum.CONCLUSION Routine postpartum intrauterine exploration is not beneficial to the mother and may even increase the risk of rupture.This case highlights a laparoscopic approach for repairing uterine rupture in the immediate postpartum period.
文摘Introduction: Vaginal adenosis is one of the rare diseases of the vagina, and almost all patients are asymptomatic. We report a case of spontaneous vaginal adenosis, which caused vesicovaginal fistula. Case Presentation: Our patient was a 25-year-old Japanese woman. She was admitted to our hospital, and her chief complaint was continuous urine flow from the vagina. We found a tumor with a vesicovaginal fistula in her vagina. Subsequent cytological analysis of vaginal smears showed a normal vaginal mucosa. The patient underwent tumor resection and a fistula patch. Pathological diagnosis was adenosis of the vagina, even though the patient had no known history of intrauterine diethylstilbestrol exposure or Müllerian developmental abnormalities. Conclusion: The clinical course of our case was a malignant tumor, which invasively bored a hole in the vaginal wall forming a vesicovaginal fistula, even though it was a benign lesion. Therefore, overzealous treatment should be avoided in this case.
文摘目的 分析老年子宫脱垂患者术后新发压力性尿失禁(de novo SUI)情况及其影响因素。方法 回顾性分析泗县人民医院2020年10月~2023年5月期间因子宫脱垂行手术治疗的60例患者,调查患者术后de novo SUI发生情况,将患者分为无de novo SUI组及de novo SUI组,通过单因素及多因素logistic回归分析影响患者术后de novo SUI发生的相关因素。结果 60例行手术治疗的子宫脱垂患者,18例患者术后发生de novo SUI,发病率为30.00%。单因素分析结果显示,体质量指数(BMI)、高血压史、巨大儿分娩史、糖尿病史、便秘史、盆腔手术史、雌激素治疗史是影响老年子宫脱垂患者术后de novo SUI的相关因素(χ^(2)=5.701、4.775、6.782、4.113、3.951、5.644、9.966,P<0.05)。多因素logistic分析结果显示,高血压史、巨大儿分娩史、糖尿病史、便秘史、盆腔手术史为老年子宫脱垂患者术后de novo SUI的独立危险因素(P<0.05)。结论 老年子宫脱垂患者术后存在一定的de novo SUI发生风险,高血压史、巨大儿分娩史、糖尿病史、便秘史、盆腔手术史为老年子宫脱垂患者术后de novo SUI的独立危险因素。
文摘Background：Although repair augmented with mesh has been proved its priority in anatomical and functional recovery after anterior compartment reconstruction,the data about posterior compartment are scarce.The aim of this study was to compare bowel functional outcome of posterior vaginal compartment repair with and without mesh in patients with pelvic organ prolapse （POP）.Methods：This was a prospective,double-blind,clinical pilot study of 22 postmenopausal women with symptomatic POP （overall POP-quantification [POP-Q] Stage Ⅲ-ⅣV） who underwent total pelvic floor reconstruction.Patients were grouped according to the use of mesh for posterior vaginal compartment repair：A mesh group and a nonmesh group.POP-Q stage,the pelvic floor impact questionnaire short form-7 （PFIQ-7） and anorectal manometry were evaluated before and 3 months after surgery.Anatomical success was defined as POP-Q Stage Ⅱ or less.A t-test was used to compare preoperative with postoperative data in the two groups.Results：Totally,17 （71％） were available for the follow-up.POP-Q measurements improved significantly compared to baseline （P ＜ 0.05） in both groups.No recurrence was observed.Subjects in both groups reported improvement in pelvic floor symptoms,and there was no significant difference in the PFIQ-7 score between groups at follow-up （P ＞ 0.05）.Compared with baseline,the nonmesh group exhibited a statistically significant decrease in anal residual pressure,a significant increase in the anorectal pressure difference during bowel movement,and a reduced rate ofdyssynergia defecation pattern （P ＜ 0.05）.Conclusions：Provided there is sufficient support for the anterior wall and apex of vagina with mesh,posterior compartment repair without mesh may be as effective as repair with mesh for anatomical recovery while providing better anorectal motor function.
