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Experiment study of effect of Valeriana officinalis var. latifolia on expression of C-Fos,C-Jun in hippocampus zone after focal cerebral ischemia 被引量:4
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作者 王云甫 严洁 +1 位作者 黄朝芬 何国厚 《中国临床康复》 CSCD 2003年第5期838-839,共2页
AIM: To study influence of Valeriana officinalis var. latifolia(VOL) on expression of C Fos,C Jun after focal cerebral ischemia. METHODS: Inducing rat model of reversible middle cerebral artery occlusion(MCAO)using Ko... AIM: To study influence of Valeriana officinalis var. latifolia(VOL) on expression of C Fos,C Jun after focal cerebral ischemia. METHODS: Inducing rat model of reversible middle cerebral artery occlusion(MCAO)using Koizumi’s intraluminal suture occlusion method.48 male rats were divided into 5 groups randomly, pseudo operation group, MCAO group, saline control group,VOL group.2 hours after MCAO, we took gastric gavage with VOL and saline, 8 hour per time, and took out of brain to test C Fos,C Jun expression immunohistochemically at the 5th day after operation.RESULTS: There was no positive cell in each hippocampus zone of normal group;we observed C Fos, C Jun positive cells in each Hippocampus zone after MCAO;Density of C Fos,C Jun positive cells of VOL group were apparently lower than that of simple ischemia group. CONCLUSION: VOL can relieve histopathological lesions after cerebral ischemia and promote protection function of rat through inhibiting the expression of C Fos,C Jun expression. 展开更多
关键词 宽叶缬草 局灶性脑缺血 海马区 C-FOS C-JUN 实验研究
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Valerian (Valeriana officinalis) extract inhibits TNF-α and iNOS gene expression in mouse LPS-activated microglial cells
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作者 Hashem Marawne Reza Mohammadhassan +1 位作者 Zahra Mohammadalipour Sedigheh Ahmadpour 《Traditional Medicine Research》 2022年第5期65-70,共6页
Background:Inflammation and damage to neurons and other cells in the nervous system can cause disorders of the central nervous system.Microglial cells are activated by pathogen infection and injury to release nitric o... Background:Inflammation and damage to neurons and other cells in the nervous system can cause disorders of the central nervous system.Microglial cells are activated by pathogen infection and injury to release nitric oxide.Valerian(Valeriana officinalis)has been used as a sedative for the treatment of neurological diseases.This study evaluated inflammation of microglial cells and tumor necrosis factorαand induced nitric oxide synthetase gene expression influenced by valerian extract.Methods:Microglial cells were isolated from mice.Lipopolysaccharide(1 ng/mL)was used to induce inflammation and nitric oxide production in cells for an hour.The inflamed cells were then treated with different concentrations(0.1,0.5,2.5,20,and 50μL/mL)of valerian alcoholic extract for 1 and 24 h.nitric oxide production and tumor necrosis factorαand induced nitric oxide synthetase gene expression were determine by Griess assay and real-time polymerase chain reaction,respectively.Results:The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed no toxicity in several concentrations of the valerian extract.In addition,concentrations from 0.1 to 2.5μL/mL significantly reduced inflammation and nitric oxide production in mouse microglial cells to levels observed in control samples.Furthermore,tumor necrosis factorαand induced nitric oxide synthetase gene expression decreased when 2.5μL/mL extract was used.Conclusion:Based on these results,it can be concluded that 2.5μL/mL valerian alcoholic extract is effective as a candidate alternative medicine for reducing inflammation and nitric oxide production and consequently,the inflammatory symptoms of neurodegeneration. 展开更多
关键词 microglial cells nitric oxide valeriana officinalis TNF-Α INOS
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HPLC-ICP-MS测定富硒芦笋中硒代蛋氨酸的不确定度评定
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作者 刘贤标 刘汉泉 +5 位作者 温志刚 邹辉 沈乐丞 李枝明 付志明 吕婷婷 《现代食品》 2024年第15期194-199,共6页
目的:对高效液相色谱-电感耦合等离子体质谱法测定富硒芦笋中硒代蛋氨酸含量的不确定度进行分析评定。方法:按照行业标准《粮谷中硒代半胱氨酸和硒代蛋氨酸的测定液相色谱-电感耦合等离子体质谱法》(NY/T 3556—2020)对富硒芦笋中硒代... 目的:对高效液相色谱-电感耦合等离子体质谱法测定富硒芦笋中硒代蛋氨酸含量的不确定度进行分析评定。方法:按照行业标准《粮谷中硒代半胱氨酸和硒代蛋氨酸的测定液相色谱-电感耦合等离子体质谱法》(NY/T 3556—2020)对富硒芦笋中硒代蛋氨酸的含量进行检测,依据《测量不确定度评定和表示》(GB/T 27418—2017)和《化学分析中测量不确定度评估指南》(RB/T 030—2020)对引入的各类不确定度分量进行分类,通过构建数学模型精确评价测定过程中的测量不确定度。结果:当富硒芦笋中硒代蛋氨酸含量为0.558 mg·kg^(-1)时,在95%的置信区间下,扩展不确定度为0.034 mg·kg^(-1)(k=2)。影响检测结果不确定度的主要因素为试样提取回收率、标准溶液配制和标准曲线拟合。结论:在测定过程中,应保证标液和器具质量,并严格按照标准化流程操作,以降低测量不确定度,获得更加准确的测量结果。 展开更多
关键词 富硒芦笋 硒代蛋氨酸 高效液相色谱-电感耦合等离子体质谱 不确定度
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用荧光显微镜技术观察药用野生稻(Oryza officinalis Wall)和转基因水稻的不亲和性 被引量:14
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作者 刘琳莉 强胜 +1 位作者 宋小玲 胡金良 《中国农业科学》 CAS CSCD 北大核心 2004年第4期469-472,T001,共5页
采用荧光显微镜技术观察转基因水稻花粉在药用野生稻柱头上的萌发及在花柱内的生长过程,以明确两者之间不亲和性发生的阶段,为判断其能否发生基因漂流提供依据。结果表明,两种转基因栽培稻(Y003和99t)的花粉在药用野生稻柱头上的萌发率... 采用荧光显微镜技术观察转基因水稻花粉在药用野生稻柱头上的萌发及在花柱内的生长过程,以明确两者之间不亲和性发生的阶段,为判断其能否发生基因漂流提供依据。结果表明,两种转基因栽培稻(Y003和99t)的花粉在药用野生稻柱头上的萌发率均比药用野生稻自花授粉的低,花粉管在花柱中的生长速度较慢,且分别在到达花柱中部(Y003)或花柱基部(99t)时停止生长,顶端异常膨大,杂交子房逐渐萎缩,结实率为0。药用野生稻与栽培稻杂交不亲和的原因是花粉管在花柱中停止生长、不能进入胚囊完成受精,在自然条件下转基因栽培稻中的外源基因向药用野生稻漂流的可能性较小。 展开更多
关键词 荧光显微镜技术 药用野生稻 转基因水稻 杂交不亲和性 基因漂流
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Greenhouse Intercropping of Green Asparagus(Asparagus officinalis Linn) and Coriander(Coriandrum sativum L.) 被引量:1
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作者 郑毅 李玉环 +2 位作者 李哲 徐海玉 凤桐 《Agricultural Science & Technology》 CAS 2016年第8期1842-1844,1848,共4页
Greenhouse cultivation in northern China has the characteristic of single mode, and the intercropping mode is not common. Greenhouse intercropping of green asparagus (Asparagus officinalis Linn) and coriander (Cori... Greenhouse cultivation in northern China has the characteristic of single mode, and the intercropping mode is not common. Greenhouse intercropping of green asparagus (Asparagus officinalis Linn) and coriander (Coriandrum sativurn L.) not only could increase vegetable kinds, but also could improve cropping index, and the asparagus growers' income could also be improved. This paper introduced the intercropping technique of green asparagus and coriander, so as to provide technical support for large-area extension of greenhouse green asparagus cultivation and break the situation of single mode for .qreenhouse cultivation. 展开更多
关键词 Asparagus officinalis linn INTERCROPPING Coriandrum Sativum l.
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太空诱变对迷迭香(Rosmarinus officinalis L.)形态参数、光合能力和次生代谢产物含量和成分的影响 被引量:2
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作者 王洪政 郭晓瑞 +3 位作者 唐中华 于景华 祖元刚 张学科 《植物研究》 CAS CSCD 北大核心 2013年第3期287-293,共7页
以未经太空诱变的迷迭香(Rosmarinus officinalis L.)为对照,研究了经返回式卫星搭载进行太空诱变后迷迭香在形态参数、光合作用以及次生产物代谢方面的变化。结果显示:太空诱变后,迷迭香优良株系的株高、冠幅、分枝数、分枝长、地上部... 以未经太空诱变的迷迭香(Rosmarinus officinalis L.)为对照,研究了经返回式卫星搭载进行太空诱变后迷迭香在形态参数、光合作用以及次生产物代谢方面的变化。结果显示:太空诱变后,迷迭香优良株系的株高、冠幅、分枝数、分枝长、地上部生物量等形态参数有了明显提高,特别是反映迷迭香可采收量的地上部生物量,比诱变前提高了1.24倍。进一步研究发现,光合能力的提升是迷迭香诱变株系形态参数发生变化的一个重要原因,因为迷迭香诱变株系的叶绿素含量、最大净光合速率、表观量子效率比对照明显提高,而光补偿点则明显下降。作为迷迭香中最主要的生物活性物质,鼠尾草酸、迷迭香酸和精油的含量在太空诱变株系中明显高于对照,并且精油中主要组分的比例也发生了显著变化。上述结果表明,太空诱变中迷迭香可获得生物产量和经济产量的有益突变,从而证明航天育种技术在迷迭香优良品种选育中是可行的。 展开更多
关键词 迷迭香 形态参数 光合作用 次生代谢
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贵州引种的迷迭香(Rosmarinus officinalis L.)中挥发油化学成分分析 被引量:16
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作者 刘兴宽 郁建平 +3 位作者 连宾 吴庆松 王德义 时磊 《贵州大学学报(农业与生物科学版)》 2002年第3期186-190,共5页
对贵州引种的迷迭香中挥发油的化学成分进行定性和定量分析 ,并与不同来源的迷迭香油的化学成分进行比较。结果表明 ,从贵州引种的迷迭香挥发油中分离出 38种成分 ,鉴定出 2 7种 ,鉴定率为71 0 5 % ,主要成分是α -蒎烯 (α -Pinene) ,... 对贵州引种的迷迭香中挥发油的化学成分进行定性和定量分析 ,并与不同来源的迷迭香油的化学成分进行比较。结果表明 ,从贵州引种的迷迭香挥发油中分离出 38种成分 ,鉴定出 2 7种 ,鉴定率为71 0 5 % ,主要成分是α -蒎烯 (α -Pinene) ,含量 2 5 .4 4% ;1,8-桉树脑 (1,8-Cineole) ,含量 2 0 .2 7% ;其次是樟脑 (Camphor) ,含量 7.6 7% ;莰烯 (Camphene) ,含量 7.10 % ;龙脑 (Borneol) ,含量 3.30 % ;β -石竹烯 (β -Caryophyllene) ,含量 3.2 3%。贵州引种的迷迭香中挥发油的化学成分主要是α -蒎烯、1,8-桉树脑 ,占挥发油检出成分的 4 5 .71%。该挥发油按化学成分分类属α -蒎烯、1. 展开更多
关键词 迷失香 引种 挥发油 化学成分 气相色谱-质谱联用 定性分析 定量分析 贵州 天然抗氧化剂
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Antibacterial activity of Rosmarinus officinalis L.alone and in combination with cefuroxime against methicillin-resistant Staphylococcus aureus 被引量:10
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作者 Naser Jarrar Awni Abu-Hijleh Kamel Adwan 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2010年第2期121-123,共3页
Objective:To determine the antimicrobial activity of rosemary(Rosmarinus of ficinalis L.) and to investigate the synergistic effects of this extract combined with ceforuxime against methicillinresistant Staphylococcus... Objective:To determine the antimicrobial activity of rosemary(Rosmarinus of ficinalis L.) and to investigate the synergistic effects of this extract combined with ceforuxime against methicillinresistant Staphylococcus aureus(MRSA).Methods:The inhibitory and bactericidal activities of rosemary ethanol extract,alone and in combination with cefuroxime,were studied.Results:The minimum inhibitory concentrations(MICs) of the ethanol extract of rosemary were in the range of 0.39-3.13 mg/mL.The minimum bactericidal concentrations(MBCs) were usually equal to or double that MICs.The antimicrobial activity of combinations of the ethanol extract of rosemary and cefuroxime indicated their synergistic effects against all MRSAs.Conclusions:The present work clearly demonstrates that rosemary has a key role in the elevation of susceptibility toβ-lactams. 展开更多
关键词 Rosmarinus officinalis l MEDICINAl plants Antibacterial activity Herb-drug interactions Plant EXTRACTS
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Ultraviolet irradiation induced oxidative stress and response of antioxidant system in an intertidal macroalgae Corallina officinalis L. 被引量:5
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作者 Lixia Li Jiqiang Zhao Xuexi Tang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2010年第5期716-722,共7页
The response of the antioxidant defense system of an intertidal macroalgae Corallina officinalis L.to different dosages of UV-B irradiation was investigated.Results showed that superoxide dimutase (SOD) and peroxida... The response of the antioxidant defense system of an intertidal macroalgae Corallina officinalis L.to different dosages of UV-B irradiation was investigated.Results showed that superoxide dimutase (SOD) and peroxidase (POX) increased and then maintained at a relatively stable level when subjected to UV-B irradiation.Catalase (CAT) activity under medium dosage of UV-B irradiation (Muv) and high dosage of UV-B irradiation (Huv) treatments were significantly decreased.Ascorbate peroxidase (APX) activity first remained unaltered and then increased in Huv treatment.In addition,the assay on isozymes was carried out using non-denaturing polyacrylamide gel electrophoresis (PAGE).The activities of some SOD isoforms were altered by UV-B.Two new bands (POX V and POX VII) appeared upon exposure to all three UV-B dosages.CAT III activity was increased by low dosage of UV-B irradiation (Luv),whereas CAT III and CAT IV disappeared when the alga was exposed to Muv and Huv.Two bands of APX (APX VI and APX VII) were increased and a new band (APX X) was observed under Huv exposure.H 2 O 2 and thiobarbituric acid reacting substance (TBARS) increased under Muv and Huv treatments.Overall,UV-B protection mechanisms are partly inducible and to a certain extent sufficient to prevent the accumulation of damage in C.officinalis. 展开更多
关键词 UV-B irradiation Corallina officinalis l. superoxide dimutase CATAlASE ascorbate peroxidase PEROXIDASE
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In vitro effects of Salvia officinalis L.essential oil on Candida albicans 被引量:2
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作者 Tularat Sookto Theerathavaj Srithavaj +2 位作者 Sroisiri Thaweboon Boonyanit Thaweboon Binit Shrestha 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2013年第5期376-380,共5页
Objective:To determine the anticandidal activities of Salvia officinalis L.(S.officinalis)essential oil against Candida albicans(C.albicans)and the inhibitory effects on the adhesion of C.albicans to polymethyl methac... Objective:To determine the anticandidal activities of Salvia officinalis L.(S.officinalis)essential oil against Candida albicans(C.albicans)and the inhibitory effects on the adhesion of C.albicans to polymethyl methacrylate(PMMA)resin surface.Methods:Disc diffusion method was first used to test the anticandidal activities of the S.officinalis L.essential oil against the reference strain(ATCC 90028)and 2 clinical strains of C.albicans.Then the minimal inhibitory concentration(MIC)and minimal lethal concentration(MLX)were determined by modified membrane method.The adhesion of C.albicans to PMMA resin surface was assessed after immersion with S.officinalis L.essential oil at various concentrations of 1XMIC.0.5XMIC and 0.25XMIC at room temperature for30 min.One-way ANOVA was used to compare the Candida cell adhesion with the pretreatment agents and Tukey's test was used for multiple comparisons.Results:5.officinalis L.essential oil exhibited anticandidal activity against all strains of C.albicans with inhibition zone ranging from 40.5 mm to 19.5 mm.The MIC and MLC of the oil were determined as 2.780 g/L against all test strains.According to the effects on C.albicans adhesion to PMMA resin surface,it was found that immersion in the essential oil at concentrations of 1XMIC(2.780 g/L).0.5XMIC(1.390 g/L)and0.25XMIC(0.695 g/L)for 30 min significantly reduced the adhesion of all 3 test strains to PMMA resin surface in a dose dependent manner(P<0.05).Conclusions:S.officinalis L.essential oil exhibited anticandidal activities against C.albicans and had inhibitory effects on the adhesion of the cells to PMMA resin surface.With further testing and development,S.officinalis essential oil may be used as an antifungal denture cleanser to prevent candidal adhesion and thus reduce the risk of candida-associated denture stomatitis. 展开更多
关键词 SAlVIA officinalis l. CANDIDA AlBICANS ESSENTIAl oil PMMA RESIN Adhesion
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Effective component from verbena officinalis L.inhibits proliferation and induces apoptosis of human choriocarcinoma JAR cells 被引量:4
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作者 徐珊 陈琪 徐昌芬 《生殖医学杂志》 CAS 2005年第B10期12-18,共7页
Objective:To examine the action of the effective component,4'-methylether-scutellarein,from Verbena officinalis L.(VOL)on the proliferation and apoptosis of human choriocarcinomaJAR cells.Methods:Cell proliferatio... Objective:To examine the action of the effective component,4'-methylether-scutellarein,from Verbena officinalis L.(VOL)on the proliferation and apoptosis of human choriocarcinomaJAR cells.Methods:Cell proliferation was measured by MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyl tetrasodium bromide,MTT]assay and the incorporation of tritiated thymidine(~3H-TdR).Apoptosis of cell was evaluated by flow cytometry(FCM)and the characteristic apoptoticDNA ladder by agarose gel electrophoresis,and the morphological changes of apoptotic JAR cellswere observed under fluorescence microscopy and electron microscopy(EM).Expressions of ap-optosis proteins,poly(ADP-ribose)polymerase(PARP)and caspase-3,-8,and -9 were deter-mined with Western blot.Results:The effective component from VOL inhibited the proliferation of JAR cells in a dose-and time-dependent manner.The treated cell cycle was arrested in S phase and an apoptotic peakwas found in S phase using FCM analysis.A typical DNA ladder appeared in the treatment groupwhen analyzed by agarose gel electrophoresis.Using fluorescence microscopy,the percentage ofapoptotic cell was 0.9%,6%,and 14% after treatments of 10,20,and 40 mg·L^(-1) of the effec-tive component,respectively,for 48 h.Typical apoptotic changes,such as condensed chromatinand presence of apoptotic bodies,were observed under EM.Treatment with effective componentfor 48 h and 72 h also induced protein expression of PARP and caspase-3,-8,and -9 as seen byWestern blot.Conclusions:The effective component from VOL inhibits cell proliferation and induces apop-tosis in human choriocarcinoma JAR cells. 展开更多
关键词 ANTI-CANCER CHORIOCARCINOMA 4’-methylether-scutellarein Verbena officinalis l. APOPTOSIS
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Chemical composition of Rosmarinus officinalis essential oil and antioxidant action against gastric damage induced by absolute ethanol in the rat 被引量:3
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作者 Christiane Takayama Felipe Meira de-Faria +7 位作者 Ana Cristina Alves de Almeida Ricardo Jose Dunder Luis Paulo Manzo Eduardo Augusto Rabelo Socca Leonia Maria Batista Marcos Jose Salvador Alba Regina Monteiro Souza-Brito Anderson Luiz-Ferreira 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2016年第8期677-681,共5页
Objective: To evaluate the antioxidant activity of the essential oil obtained from Rosmarinus officinalis(R. officinalis) in ethanol-induced gastric ulcer model in vivo.Methods: The antioxidant properties of the essen... Objective: To evaluate the antioxidant activity of the essential oil obtained from Rosmarinus officinalis(R. officinalis) in ethanol-induced gastric ulcer model in vivo.Methods: The antioxidant properties of the essential oil obtained from R. officinalis were evaluated against gastric injury induced by absolute ethanol. Gastric tissues were prepared to enzymatic assays. The levels of glutathione, lipid peroxides, and the activities of glutathione peroxidase, superoxide dismutase and myeloperoxidase were measured.Results: Ethanol produced severe hemorrhagic lesions in the stomach with ulcerative lesion of(140.2 ± 37.2) mm2. In animals pretreated with essential oil of R. officinalis(50 mg/kg, p.o.), a significant inhibition of mucosal injury of(21.2 ± 7.1) mm2(84%inhibition) was observed. The essential oil of R. officinalis protected the gastric mucosa probably by modulating the activities of the enzymes(superoxide dismutase and glutathione peroxidase) and increasing or maintaining the levels of glutathione. In addition,lipid peroxides levels were reduced. The essential oil of R. officinalis was analyzed by gas chromatography–mass spectrometer and the main constituents were cineole(28.5%),camphor(27.7%) and alpha-pinene(21.3%).Conclusions: We suggest that the monoterpenes present in the essential oil obtained from R. officinalis may be among the active principles responsible for the antioxidant activity shown by essential oil of R. officinalis. 展开更多
关键词 Rosmarinus officinalis l. Antioxidant activity Gastric ulcer Antioxidant enzymes
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Essential oil of <i>Thymus vulgaris</i>L. and <i>Rosmarinus officinalis</i>L.: Gas chromatography-mass spectrometry analysis, cytotoxicity and antioxidant properties and antibacterial activities against foodborne pathogens 被引量:2
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作者 Hanene Miladi Rihab Ben Slama +3 位作者 Donia Mili Sami Zouari Amina Bakhrouf Emna Ammar 《Natural Science》 2013年第6期729-739,共11页
The essential oil composition of Thymus vulgaris L. and Rosmarinus officinalis L. endemic to France were determined by GC and GC-MS. Oils were assessed for their cytotoxic, antioxidant and antimicrobial activity. 31 a... The essential oil composition of Thymus vulgaris L. and Rosmarinus officinalis L. endemic to France were determined by GC and GC-MS. Oils were assessed for their cytotoxic, antioxidant and antimicrobial activity. 31 and 37 different compounds were identified representing 99.64% and 99.38% of the thyme and rosemary oils respectively, where oxygenated monoterpenes constituted the main chemical class. Thymol (41.33%) and 1.8-cineole (24.10%) were identified as the main constituents of T. vulgaris L. and R. officinalis L., respectively. Essential oils (EOs) of selected plant species were evaluated for their in vitro cytotoxicity against the human lung adenocarcinoma epithelial cell line (A549). Cytotoxicity was measured using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphynyltetra-zolium bromide] colorimetric assay. Dose-dependent studies revealed IC50 of 8.50 ± 0.01 μg/mL and 10.50 ± 0.01 μg/mL after 72 h on the A549 cells for R. officinalis L. and T. vulgaris L., respectively. Antioxidant activity was determined using a quantitative DPPH (1,1-diphenyl- 2-picryl hydrazyl) assay. Thymus and rosemary EOs exhibited effective radical scavenging capacity with 50% inhibitory concentration (IC50) of 437 ± 5.46 μg/mL and 189 ± 2.38 μg/mL respectively and therefore acts as a natural antioxidant agent. The antimicrobial activity of these species has also been studied against several foodborne pathogens and food isolated Salmonella spp. including S. enteritidis of significant importance. According to the results, T. vulgaris L. showed higher bactericidal effect than those from R. officinalis L. These results suggest that the essential oil from T. vulgaris L. and R. officinalis L. have potential to be used as a natural cytotoxic, antioxidant and antimicrobial agent in food processing. 展开更多
关键词 THYMUS VUlGARIS l. Rosmarinus officinalis l. GC–MS CYTOTOXICITY Antioxidant ACTIVITY Antimicrobial ACTIVITY
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Hepatoprotective and Antioxidant Effects of <i>Salvia officinalis</i>L. Hydroalcoholic Extract in Male Rats 被引量:1
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作者 Khakpour Shahrzad Najari Mahya +3 位作者 Tokazabani Belasei Fatemeh Khosravi Maryam Farhadi Belasei Mohammadreza Mahsa Hadipour Jahromy 《Chinese Medicine》 2014年第2期130-136,共7页
The antioxidant effects of Salvia officinalis L. hydroalcoholic extract and also its hepatoprotective effects in male rats were evaluated. Salvia officinalis L. extract was administered intraperitoneally for 28 days. ... The antioxidant effects of Salvia officinalis L. hydroalcoholic extract and also its hepatoprotective effects in male rats were evaluated. Salvia officinalis L. extract was administered intraperitoneally for 28 days. Serum levels of aspartate aminotransferase, alanine transferase and alkaline phosphates in rats coadministered with both isoniazid (INH) 50 mg/kg and Salvia officinalis L. extract at 250 mg/kg showed significant reduction when compared to INH group, but administration of Salvia officinalis L. extract 250 mg/kg alone and with INH has alleviated gamma-glutamyl transferase comparing to INH receiving rats. After treatment of rats with INH 50mg/kg, severe tissue necrosis, and inflammation of central vein in liver and lymphocyte proliferation were observed;in the experimental group receiving Salvia officinalis L. extract (250 mg/kg) mild dilution in central vein and sinusoids in liver were seen. In rats coadministered with Salvia officinalis L. extract 250 mg/kg and INH low sinusoids dilution was indicated. It seems that Salvia officinalis L. extract exhibited anti-oxidative and hepatoprotective effects. 展开更多
关键词 SAlVIA officinalis l. ANTIOXIDANT Effects Rat
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The Anatomical Structure of Vegetative Organs <i>Lavandula officinalis</i>Chaix in the Introduction of Tashkent Botanical Garden
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作者 Durdona K. Fakhriddinova Tashkhanim R. Rakhimova +3 位作者 Feruza M. Dusmuratova Guljan M. Duschanova Sodikjon H. Abdinazarov Ismatjon N. Samadov 《American Journal of Plant Sciences》 2020年第4期578-588,共11页
The article presents the results of research carried out by light microscopy of the anatomical structure of vegetative organs (leaf, root and stem) Lavandula officinalis Chaix of the family Lamiaceae introduced into t... The article presents the results of research carried out by light microscopy of the anatomical structure of vegetative organs (leaf, root and stem) Lavandula officinalis Chaix of the family Lamiaceae introduced into the conditions of the Tashkent Botanical Garden of the Republic of Uzbekistan. Our studies have shown that the presence or absence of trichomes can serve as an informative sign. In L. officinalis trichomes are simple unicellular two-terminal;three-terminal and multi-terminal. Lavender leaf hairs accumulate essential oils under the cuticle of secreting cells, the cuticle are tightly attached to the membranes of the secretory cells due to the absence of excretion products. Based on the results of a study of the morphological and anatomical features of the leaf of L. officinalis, it has been established that the forms of covering hairs and essential oil glands are of diagnostic value. In the epidermal, palisade and cortex parenchymal cells of the vegetative organs, yellow-orange essential (lavender) oil is noted. Identified diagnostic features that can be used in taxonomy and can be used to identify plant materials can also be used to develop indicators of authenticity in this type of raw material. 展开更多
关键词 Anatomy Vegetative Organs INTRODUCTION l. officinalis Tashkent BOTANICAl Garden
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Establishment of Rapid Propagation System for Saponaria officinalis L. Progeny After EMS Mutation
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作者 Yang Xue Wang Jin-gang +4 位作者 Yang Tao Li Shan-shan Ren Hong-wei Fu Guo-hua Gong Shu-fang 《Journal of Northeast Agricultural University(English Edition)》 CAS 2012年第1期21-26,共6页
Saponaria officinalis L. seeds were treated with different concentrations of chemical mutagen to determine the impact of media with various hormone concentrations on the induction of mutated (Saponaria officinalis L.... Saponaria officinalis L. seeds were treated with different concentrations of chemical mutagen to determine the impact of media with various hormone concentrations on the induction of mutated (Saponaria officinalis L.) seeds' adventitious buds and rooting culture by using tissue culture technology. The results indicated that the optimum concentration of the chemical mutagen-EMS was 0.3%; the optimum induction medium was MS+6-BA 1.5 mg · L^-1, NAA 0.1 mg · L^-1 and the optimum rooting medium was MS+0.05 mg · L^-1 NAA. 展开更多
关键词 Saponaria officinalis l. chemical mutagen tissue culture
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Effects of Salt Stress on Growth and Ion Distribution in Seedlings of Asparagus officinalis L.
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作者 Yanpo CAO Peng DAI Suying DAI 《Agricultural Biotechnology》 CAS 2018年第4期69-72,75,共5页
An Asparagus officinalis L. cultivar NJ978 was used to study the growing rate, salt ion content and mineral ion uptake and distribution in plants under salt stress. The results showed that salt stress significantly in... An Asparagus officinalis L. cultivar NJ978 was used to study the growing rate, salt ion content and mineral ion uptake and distribution in plants under salt stress. The results showed that salt stress significantly inhibited seedling growth and there was a negative relationship between seedling growth and salt concentration. The seedlings growth showed no significant decrease under low salt stress (NaCl≤50 mmol/L), while high concentrations of NaCl led to detrimental effects on the growth of A. officinalis L.. With the increase of NaCl concentration, the Na + content increased gradually in A. officinalis L. roots, stems and leaves while the K^+ and Ca^2+ contents had slow decreases. Under low salt stress, the seedlings of A. officinalis L. could prevent Na + from transporting to aerial part by withholding Na + in root, which could keep the balance of ions in aerial part, but under high salt stress (NaCl 〉 100 mmol/L), the aerial part of seedlings accumulated superabundance of Na +, which limited the uptake of K^+ and Ca^2+ , so seedlings were damaged heavily, which manifested as the gradual decrease of K^+/Na + and Ca^2+ /Na + in the roots and aerial part of the seedlings. The selective absorption of K^+, Ca^2+ and Na + (AS K,Na and AS Ca,Na ) was significantly increased with increased salinity, while the selective transportation (TS K,Na and TS Ca,Na ) increased at first and then decreased with the increased salinity. Therefore, the strong ability of salt exclusion and the regionalized distribution of ions in roots, stems and leaves could be one of the salt tolerance mechanisms of A. officinalis L. 展开更多
关键词 Asparagus officinalis l. Salt stress GROWTH Ion distribution
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Estimation of Some Plant Secondary Products in Urtica dioica L., Viola odorata L. and Melissa officinalis L. Naturally Grown in Hawraman-Kurdistan Region of Iraq
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作者 Abdulsalam Abdulrahman Rasool Kadhm Abdullah Muhammad 《Journal of Agricultural Science and Technology(B)》 2013年第7期480-486,共7页
The plant aerial parts of three species, Urtica dioica L., Viola odorata L. and Melissa officinalis L. were collected at randomly-different locations, according to altitudes in May 2010. The aerial parts of Urtica wer... The plant aerial parts of three species, Urtica dioica L., Viola odorata L. and Melissa officinalis L. were collected at randomly-different locations, according to altitudes in May 2010. The aerial parts of Urtica were collected within three replications from different locations: Biare 1,090 masl (meters above sea level), Tawile 1,450 masl and Awiser 1,680 masl. The aerial parts of Viola and Melissa were collected randomly within three replications at different locations: Biare 1,090 masl, Degashikhan 1,250 masl and Tawile 1,450 masl. The extracts of the aerial parts of these species were purified by filtrations for several times in preparation for HPLC analyses. The chromatograms of Urtica indicated the presence of five major important alkaloid components (fragrine, benzylisoquinoline, scopoletin, glucoquinone and dotriacotaine) and ten major important phenolic compounds (formic acid, tannin, chlorogenic acid, caffeoylmalic acid, anthocyanine, quercetin, zeaxanthin, luetin epoxide, coumarine and vanillin). All the concentrations of alkaloid and phenolic compounds were increased significantly due to higher altitudes, except that of alkaloid dotriacotaine. The chromatograms of Viola indicated the presence of four major important alkaloid components (violine, isoquinoline, cycloviolacin and luteolin-3-glucoronide) and ten major important phenolic compounds (formic acid, tannin, chlorogenic acid, caffeoylmalic acid, anthocyanine, quercetin, zeaxanthin, luetin epoxide, coumarine and vanillin). The results of the influence of altitudes showed that the concentrations of all alkaloids and phenolic compounds were increased significantly due to higher altitudes, except that of the alkaloid luteolin-3-glucoronide and the phenolic compounds zeaxanthin and luetin epoxide. The chromatograms of Melissa indicated the presence of five major essential oils (pinene, linalool, citronellol, geraniol and rosmarinic acid). Their quantitative evaluations were influenced by altitudes indicating that the concentrations of all oils were increased significantly due to the higher altitude, except that of the pinene. 展开更多
关键词 Urtica dioica l. Viola odorata l. Melissa officinalis l. AlTITUDES secondary products medicinal plants
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Anti-tumor activity of Sanguisorba officinalis L.in non-small cell lung cancer and induced apoptosis via PI3K/Akt/mTOR signaling pathway
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作者 LI Hong LI Jing WU Jian-ming 《中国药理学与毒理学杂志》 CAS 北大核心 2021年第10期732-733,共2页
OBJECTIVE To investigate the pharmacological effect and mechanism of Sanguisorba officinalis L.(SOL)in non-small cell lung cancer(NSCLC)in vitro and in vivo based on network pharmacology.METHODS Network pharmacology w... OBJECTIVE To investigate the pharmacological effect and mechanism of Sanguisorba officinalis L.(SOL)in non-small cell lung cancer(NSCLC)in vitro and in vivo based on network pharmacology.METHODS Network pharmacology was used to analyze the improving effect of SOL on NSCLC and possible targets.Cell counting kit 8(CCK-8)and 5-ethynyl-2′-deoxyuridine(EdU)staining,Western blotting,flow cytometry of AnnexinⅤ/PI,Hoechst 33342/PI staining detection and immunofluorescence were utilized in vitro.H&E staining,immunohistochemistry staining and Western blotting were performed in vivo.RESULTS Based on network prediction,we analyzed the 208 common targets of SOL and NSCLC.36 core targets in 208 common targets were obtained through cytoscape analysis.And the top 10 core targets included Akt,mTOR,EGFR,etc..KEGG analysis showed that PI3K-Akt signaling pathway was the most likely pathway.Furthermore,the mechanism study found that SOL could activate the PI3K/Akt/mTOR signaling pathway in vitro and in vivo.The anti-proliferative effect of SOL in A549 and H1299 cells was measured and validated by CCK-8 and EdU assay.Immunohistochemical results of Ki67 showed that SOL effectively inhibited tumor growth in vivo.SOL also significantly inhibited the migration and invasion of A549 and H1299 cells.SOL significantly increased the percentage of cells with PI signal in A549 and H1299,and the process of cell death of A549 cells indicated that SOL induced apoptosis.The PARP-1 and caspase-3 in A549 and H1299 were found to be activated in a dose manner.The results in vivo were consistent with those in vitro.CONCLUSION SOL-induced,caspase-3-mediated apoptosis via the induction of the PI3K/Akt/mTOR signaling pathway in NSCLC,which further clarified the mechanism of SOL in the inhibition of NSCLC,and thereby provided a possibility for SOL to serve as a novel therapeutic agent for NSCLC in the future. 展开更多
关键词 Sanguisorba officinalis l. non-small cell lung cancer network pharmacology PARP-1 PI3K/AKT/MTOR
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The Effect of N-Fertilization on Rosmarinus Officinalis L. (An Upright Variety) Yield in Central Greece
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作者 Eleni Wogiatzi-Kamwoukou Nickolaos Gougoulias +2 位作者 Zoi Papadouli Baia Theodosiou Kyriakos D Giannoulis 《Journal of Food Science and Engineering》 2017年第3期141-146,共6页
The effect of three different N-fertilization levels (NI: 625, N2:385 and N3:770 kg hal; where in case of N1 was used the 3-6-10+3MGO+30% OM and in cases of N2-N3 the 26-0-0 fertilizers) on fresh and dry weight... The effect of three different N-fertilization levels (NI: 625, N2:385 and N3:770 kg hal; where in case of N1 was used the 3-6-10+3MGO+30% OM and in cases of N2-N3 the 26-0-0 fertilizers) on fresh and dry weight of the perennial Rosmarinus officinalis (upright cultivar) was investigated during the 2nd year after establishment at the Experimental Farm of the Technological Educational Institute of Thessaly in Greece (TEI; Larissa plain) in 2015. It is well documented that the crop reaches its potential yield on the third year of cultivation and continues producing biomass for as long as eight years. Complete weather data (air temperature, radiation, air humidity, precipitation) were recorded hourly in an automatic meteorological station, which was installed to the experimental farm of TEI. Upon harvest (November 3rd 2015), the crop reached a maximum fresh yield of 11.67 tons per hectare and dry yield of 4.3, respectively. The average fresh weight was 8.2, 8.4 and 8.9 t hal and the dry weight were 2.6, 3.1 and 3.2 t ha-1 for the N1, N2 and N3 levels, respectively. Furthermore the higher moisture content was observed in the case of N1 level (68%). Therefore, the above data show that rosemary cultivation could be a promising alternative crop, especially in case of the consideration that average selling price of dry rosemary in Greece is 3.5 ∈ kg^-1 and the average gross income exceeds the amount of 10,000 ∈ ha^-1. 展开更多
关键词 Rosmarinus officinalis l. FERTIlIZATION upright variety fresh yield dry yield.
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