目的用微波加热作丽春红-苦味酸-维多利亚蓝染色分析。方法采用丽春红-苦味酸-维多利亚蓝（Ponceau-Victoria blue B）染色分实验组（用微波加热）与对照组（不用微波,只用原法分析）,共检测子宫肌瘤10例,女性乳腺纤维腺瘤10例及男性前...目的用微波加热作丽春红-苦味酸-维多利亚蓝染色分析。方法采用丽春红-苦味酸-维多利亚蓝（Ponceau-Victoria blue B）染色分实验组（用微波加热）与对照组（不用微波,只用原法分析）,共检测子宫肌瘤10例,女性乳腺纤维腺瘤10例及男性前列腺增生30例。结果用Ponceau-Victoria blue B者,不论是实验组还是对照组,其胶原纤维呈红色,肌纤维呈黄色;用微波加热者,染色时间缩短,保存时间延长,染色3-4周后照相,彩色反差仍明显。结论微波加热适用于Ponceau-Victoria blue B染色,染色后切片保存的时间较长;胶原纤维（红色）与平滑肌纤维（黄色）的反差也不错,有助于鉴别两种不同来源的肿瘤。展开更多
目的脑信号蛋白4D(Sema4D)是发现最早的免疫信号素,可能参与骨质疏松症的发生和发展。文章通过检测骨质疏松小鼠血清中可溶性脑信号蛋白4D(sSema4D)的水平,探寻sSema4D与骨质疏松症发病的关系。方法选取3月龄SPF级野生型C57小鼠,随机抽...目的脑信号蛋白4D(Sema4D)是发现最早的免疫信号素,可能参与骨质疏松症的发生和发展。文章通过检测骨质疏松小鼠血清中可溶性脑信号蛋白4D(sSema4D)的水平,探寻sSema4D与骨质疏松症发病的关系。方法选取3月龄SPF级野生型C57小鼠,随机抽样法分为两组:骨质疏松组(n=50):每天给予0.9%维甲酸(90 mL/kg)灌胃共21 d;对照组(n=50):每天灌以0.9%氯化钠注射液(90 mL/kg)。应用Micro-CT对小鼠胫骨近端干骺端进行扫描并行三维重建,观察骨小梁数目、结构、形态、粗细、排列及走向情况;使用Inveon Research Work Place软件对胫骨近端干骺端兴趣区域的CT值进行处理,测量小鼠骨小梁数目、骨小梁厚度、骨小梁体积分数、骨小梁分离度。采用硬组织切片技术切片,苦味酸-酸性品红法染色,显微镜下观察股骨、胫骨组织骨胶原纤维病理学改变。采用酶联免疫吸附法检测小鼠血清中sSema4D水平。结果骨质疏松症组小鼠的骨小梁数目、骨小梁厚度、骨小梁体积分数较对照组明显减少(5.8 mm vs 7.0 mm,0.06 mm vs 0.08 mm,0.3%vs0.5%,P<0.01),骨小梁分离度明显增大[(0.69±0.13)mm vs(0.54±0.12)mm,P<0.05]。骨组织病理学表现:苦味酸-酸性品红法染色镜下显示骨质疏松组小鼠的胶原纤维排列疏松、稀少,纤维结构紊乱,部分胶原纤维连续性变差,溶解及断裂,骨髓腔增大等;血清中sSema4D水平:骨质疏松症组小鼠血清sSema4D较对照组的sSema4D显著升高(P<0.01)。结论 sSema4D可能促使骨质疏松症的发生。展开更多
BACKGROUND Dentinogenic ghost cell tumor(DGCT) is an uncommon locally invasive odontogenic neoplasm. It is considered to be a solid variant of calcifying odontogenic cyst(COC). This tumor makes up for only 2%-14% of a...BACKGROUND Dentinogenic ghost cell tumor(DGCT) is an uncommon locally invasive odontogenic neoplasm. It is considered to be a solid variant of calcifying odontogenic cyst(COC). This tumor makes up for only 2%-14% of all COCs and less than 0.5% of all odontogenic tumors which owes to its rarity. The purpose of this paper was to describe a case of DGCT and the treatment adopted in our case,and to provide a review of this case in the indexed literature.CASE SUMMARY In this article, we discussed a case of 18 year old male who reported with a chief complaint of a recurrent swelling and dull aching pain in upper left back region of the jaw. Computed tomography scan was carried out which revealed hypodense lesion with a few hyperdense flecks within it suggesting the presence of calcification. On incisional biopsy, diagnosis of COC was given. After segmental resection of the lesion, histopathogically odontogenic epithelium was noted along with calcifications, ghost cells and dentinoid material. Special staining was done with van Gieson and it showed pink areas of dentinoid material and yellow colour represented ghost cells. Hence, amalgamation of careful clinical examination, use of advanced radiographic imaging and detailed histopathological examination confirmed the diagnosis of DGCT. The patient was followed up for one year and there was no recurrence of the lesion or signs of any residual tumor.CONCLUSION Radical treatment should be carried out along with mandatory long-term follow up in order to avoid recurrence in aggressive lesions.展开更多
文摘目的用微波加热作丽春红-苦味酸-维多利亚蓝染色分析。方法采用丽春红-苦味酸-维多利亚蓝（Ponceau-Victoria blue B）染色分实验组（用微波加热）与对照组（不用微波,只用原法分析）,共检测子宫肌瘤10例,女性乳腺纤维腺瘤10例及男性前列腺增生30例。结果用Ponceau-Victoria blue B者,不论是实验组还是对照组,其胶原纤维呈红色,肌纤维呈黄色;用微波加热者,染色时间缩短,保存时间延长,染色3-4周后照相,彩色反差仍明显。结论微波加热适用于Ponceau-Victoria blue B染色,染色后切片保存的时间较长;胶原纤维（红色）与平滑肌纤维（黄色）的反差也不错,有助于鉴别两种不同来源的肿瘤。
文摘目的脑信号蛋白4D(Sema4D)是发现最早的免疫信号素,可能参与骨质疏松症的发生和发展。文章通过检测骨质疏松小鼠血清中可溶性脑信号蛋白4D(sSema4D)的水平,探寻sSema4D与骨质疏松症发病的关系。方法选取3月龄SPF级野生型C57小鼠,随机抽样法分为两组:骨质疏松组(n=50):每天给予0.9%维甲酸(90 mL/kg)灌胃共21 d;对照组(n=50):每天灌以0.9%氯化钠注射液(90 mL/kg)。应用Micro-CT对小鼠胫骨近端干骺端进行扫描并行三维重建,观察骨小梁数目、结构、形态、粗细、排列及走向情况;使用Inveon Research Work Place软件对胫骨近端干骺端兴趣区域的CT值进行处理,测量小鼠骨小梁数目、骨小梁厚度、骨小梁体积分数、骨小梁分离度。采用硬组织切片技术切片,苦味酸-酸性品红法染色,显微镜下观察股骨、胫骨组织骨胶原纤维病理学改变。采用酶联免疫吸附法检测小鼠血清中sSema4D水平。结果骨质疏松症组小鼠的骨小梁数目、骨小梁厚度、骨小梁体积分数较对照组明显减少(5.8 mm vs 7.0 mm,0.06 mm vs 0.08 mm,0.3%vs0.5%,P<0.01),骨小梁分离度明显增大[(0.69±0.13)mm vs(0.54±0.12)mm,P<0.05]。骨组织病理学表现:苦味酸-酸性品红法染色镜下显示骨质疏松组小鼠的胶原纤维排列疏松、稀少,纤维结构紊乱,部分胶原纤维连续性变差,溶解及断裂,骨髓腔增大等;血清中sSema4D水平:骨质疏松症组小鼠血清sSema4D较对照组的sSema4D显著升高(P<0.01)。结论 sSema4D可能促使骨质疏松症的发生。
文摘BACKGROUND Dentinogenic ghost cell tumor(DGCT) is an uncommon locally invasive odontogenic neoplasm. It is considered to be a solid variant of calcifying odontogenic cyst(COC). This tumor makes up for only 2%-14% of all COCs and less than 0.5% of all odontogenic tumors which owes to its rarity. The purpose of this paper was to describe a case of DGCT and the treatment adopted in our case,and to provide a review of this case in the indexed literature.CASE SUMMARY In this article, we discussed a case of 18 year old male who reported with a chief complaint of a recurrent swelling and dull aching pain in upper left back region of the jaw. Computed tomography scan was carried out which revealed hypodense lesion with a few hyperdense flecks within it suggesting the presence of calcification. On incisional biopsy, diagnosis of COC was given. After segmental resection of the lesion, histopathogically odontogenic epithelium was noted along with calcifications, ghost cells and dentinoid material. Special staining was done with van Gieson and it showed pink areas of dentinoid material and yellow colour represented ghost cells. Hence, amalgamation of careful clinical examination, use of advanced radiographic imaging and detailed histopathological examination confirmed the diagnosis of DGCT. The patient was followed up for one year and there was no recurrence of the lesion or signs of any residual tumor.CONCLUSION Radical treatment should be carried out along with mandatory long-term follow up in order to avoid recurrence in aggressive lesions.
