Objective To study the inhibitory effect of antisense endothelin receptor A (ET-AR) on the proliferation of the vascular smooth muscle cells. Methods The sense, antisense and mismatched ODNs for ET-AR were designed an...Objective To study the inhibitory effect of antisense endothelin receptor A (ET-AR) on the proliferation of the vascular smooth muscle cells. Methods The sense, antisense and mismatched ODNs for ET-AR were designed and synthetized. The study was carried out using MTT method and binding assays. Results ET-AR-ODNs could move successfully across VSMC membranes. Photo-absorption in the MTT test was reduced significantly (P<0.05) in the antisense group at 5μmol/L; the reduction of CPM also occurred in the 125 I-ET-1 specific binding assay; and the sense and mismatched ODNs groups did not show this reduction. Conclusion Our study suggested that the antisense oligomers inhibited the proliferation of VSMCs by hindering the translation of target mRNA and by reducing the production of related protein.展开更多
文摘Objective To study the inhibitory effect of antisense endothelin receptor A (ET-AR) on the proliferation of the vascular smooth muscle cells. Methods The sense, antisense and mismatched ODNs for ET-AR were designed and synthetized. The study was carried out using MTT method and binding assays. Results ET-AR-ODNs could move successfully across VSMC membranes. Photo-absorption in the MTT test was reduced significantly (P<0.05) in the antisense group at 5μmol/L; the reduction of CPM also occurred in the 125 I-ET-1 specific binding assay; and the sense and mismatched ODNs groups did not show this reduction. Conclusion Our study suggested that the antisense oligomers inhibited the proliferation of VSMCs by hindering the translation of target mRNA and by reducing the production of related protein.
