Objective:To explore inhibition effects of veliparib as PARP inhibitor combined doxorubicin for BEL-7404 proliferation of human liver cancer cell line.Methods:BEL-7404 was taken as the object of study and conventional...Objective:To explore inhibition effects of veliparib as PARP inhibitor combined doxorubicin for BEL-7404 proliferation of human liver cancer cell line.Methods:BEL-7404 was taken as the object of study and conventional culture was performed.It waslreated by doxorubicin and(or) veliparib after 24 h.Cell proliferation rate was detected by four methyl thiazolyl tetrazolium(MTT) assay,cell apoptosis was measured with annexin V-F1TC/PI double staining method by flow cytometry,DNA damage degree evaluation by single cell gel electrophoresis assay,and cytosolic C levels of the mitochondrial and cytosol by polyacrylamide gel electrophoresis(Western blotting).Results:Cell proliferation rate of doxorubicin combined veliparib group was lower than that of the control group and doxorubicin alone treated group significantly(P<0.01),the apoptosis rate was significantly higher than that of the control group and doxorubicin alone treated group(P<0.05).At the same time,DNA damage level of doxorubicin combined with veliparib group was significantly higher than doxorubicin alone treatment group and the control group(P<0.01),and cytochrome C in the cytosol was significantly higher than that of control group and doxorubicin alone treated group(P<0.01).Conclusions:Veliparib,PARP inhibitor could inhibit PARP activity,block tumor cell DNA repair,and have significant sensitizing effect for hepatocellular carcinoma cell line BEL-7404 treated with doxorubicin.This might provide a new target for clinical treatment of hepatic carcinoma.展开更多
基金supported by grants from Natural Science Foundation of Liaoning Province(No.201102006)
文摘Objective:To explore inhibition effects of veliparib as PARP inhibitor combined doxorubicin for BEL-7404 proliferation of human liver cancer cell line.Methods:BEL-7404 was taken as the object of study and conventional culture was performed.It waslreated by doxorubicin and(or) veliparib after 24 h.Cell proliferation rate was detected by four methyl thiazolyl tetrazolium(MTT) assay,cell apoptosis was measured with annexin V-F1TC/PI double staining method by flow cytometry,DNA damage degree evaluation by single cell gel electrophoresis assay,and cytosolic C levels of the mitochondrial and cytosol by polyacrylamide gel electrophoresis(Western blotting).Results:Cell proliferation rate of doxorubicin combined veliparib group was lower than that of the control group and doxorubicin alone treated group significantly(P<0.01),the apoptosis rate was significantly higher than that of the control group and doxorubicin alone treated group(P<0.05).At the same time,DNA damage level of doxorubicin combined with veliparib group was significantly higher than doxorubicin alone treatment group and the control group(P<0.01),and cytochrome C in the cytosol was significantly higher than that of control group and doxorubicin alone treated group(P<0.01).Conclusions:Veliparib,PARP inhibitor could inhibit PARP activity,block tumor cell DNA repair,and have significant sensitizing effect for hepatocellular carcinoma cell line BEL-7404 treated with doxorubicin.This might provide a new target for clinical treatment of hepatic carcinoma.
