对四川、浙江、安徽、河北、辽宁等地不同梨品种采集的6个梨黑星菌(Venturianashicola Tanaka et Yamamoto)的单孢分离物,采用鉴别寄主进行致病力测定和菌体酯酶同功酶测定。结果表明:在我国栽培梨上的黑星菌是存在生理分化的。并可将供...对四川、浙江、安徽、河北、辽宁等地不同梨品种采集的6个梨黑星菌(Venturianashicola Tanaka et Yamamoto)的单孢分离物,采用鉴别寄主进行致病力测定和菌体酯酶同功酶测定。结果表明:在我国栽培梨上的黑星菌是存在生理分化的。并可将供试6个分离物分成4个类型。展开更多
The super line 03-04-034, which results from a cross between Pyrus bretchneideri cv Jingbaili and P.ussuriensis cv Yali, is highly resistant to pear scab.Specimens were inoculated with Venturia nashicola to generate a...The super line 03-04-034, which results from a cross between Pyrus bretchneideri cv Jingbaili and P.ussuriensis cv Yali, is highly resistant to pear scab.Specimens were inoculated with Venturia nashicola to generate a database of expressed sequence tags(ESTs) to be used as resources for transcripts induced in inoculated and uninoculated leaves. After performing subtractive suppression hybridization using cDNA of the inoculated and uninoculated leaves harvested 51 h after inoculation, 100 clones from the uninoculated and 100 clones from the inoculated cDNA library were selected and sequenced. BLASTN searches of each cDNA library against GenBank revealed eight unique sequences associated with the defense response, comprising four ESTs from the uninoculated cDNA library(ubiquitin extension protein, subtilisin-like protease, basic helix-loop-helix(bHLH) transcription factor and aspartic endopeptidase) and four ESTs from the inoculated cDNA library(S-adenosylmethionine synthase(SAMS), heat shock factor(HSF) domain class transcription factor, RNA-binding protein and polyphenol oxidase 5 precursor).Quantitative fluorescence real-time polymerase chain analysis revealed that the transcripts for SAMS and subtilisin-like protease were highly expressed in the incompatible interaction. The SAMS gene was more highly expressed in inoculated leaves than uninoculated leaves, while the reverse was true for subtilisin-like protease. The SAMS and subtilisin-like protease genes have been found to be associated with plant defense mechanisms. Further studies of greater detail are required to understand how these genes are expressed and regulated during infection with V.nashicola.展开更多
为了解梨黑星病抗性遗传规律并为梨黑星病抗性育种早期选择提供理论依据,以新苹梨(梨黑星病抗性品种)×雪花梨(梨黑星病易感品种)的杂交后代为试材,进行了田间自然感病和棚内接种梨黑星病(Venturia nashicolaTanak et Yamamota)病...为了解梨黑星病抗性遗传规律并为梨黑星病抗性育种早期选择提供理论依据,以新苹梨(梨黑星病抗性品种)×雪花梨(梨黑星病易感品种)的杂交后代为试材,进行了田间自然感病和棚内接种梨黑星病(Venturia nashicolaTanak et Yamamota)病菌后的抗性调查,并进行了AFLP分子标记的研究。结果表明,棚内接种梨黑星病菌后,杂交后代抗感分离规律符合双假测交的遗传规律;研究建立的AFLP分子标记体系可以用于梨黑星病的抗性基因标记,并初步找到了与梨黑星病抗性基因相关的分子标记。展开更多
基金Supported by the Science and Technology Plan Projects of Hebei Province(16226312D-3)the Changli Comprehensive Experimental Station of National Pearindustry Technology Research System of(CARS-29-23)Science and Technology Plan Innovation Team Projects of Hebei Province(494-0402-TBN-OG4L)
文摘The super line 03-04-034, which results from a cross between Pyrus bretchneideri cv Jingbaili and P.ussuriensis cv Yali, is highly resistant to pear scab.Specimens were inoculated with Venturia nashicola to generate a database of expressed sequence tags(ESTs) to be used as resources for transcripts induced in inoculated and uninoculated leaves. After performing subtractive suppression hybridization using cDNA of the inoculated and uninoculated leaves harvested 51 h after inoculation, 100 clones from the uninoculated and 100 clones from the inoculated cDNA library were selected and sequenced. BLASTN searches of each cDNA library against GenBank revealed eight unique sequences associated with the defense response, comprising four ESTs from the uninoculated cDNA library(ubiquitin extension protein, subtilisin-like protease, basic helix-loop-helix(bHLH) transcription factor and aspartic endopeptidase) and four ESTs from the inoculated cDNA library(S-adenosylmethionine synthase(SAMS), heat shock factor(HSF) domain class transcription factor, RNA-binding protein and polyphenol oxidase 5 precursor).Quantitative fluorescence real-time polymerase chain analysis revealed that the transcripts for SAMS and subtilisin-like protease were highly expressed in the incompatible interaction. The SAMS gene was more highly expressed in inoculated leaves than uninoculated leaves, while the reverse was true for subtilisin-like protease. The SAMS and subtilisin-like protease genes have been found to be associated with plant defense mechanisms. Further studies of greater detail are required to understand how these genes are expressed and regulated during infection with V.nashicola.
文摘为了解梨黑星病抗性遗传规律并为梨黑星病抗性育种早期选择提供理论依据,以新苹梨(梨黑星病抗性品种)×雪花梨(梨黑星病易感品种)的杂交后代为试材,进行了田间自然感病和棚内接种梨黑星病(Venturia nashicolaTanak et Yamamota)病菌后的抗性调查,并进行了AFLP分子标记的研究。结果表明,棚内接种梨黑星病菌后,杂交后代抗感分离规律符合双假测交的遗传规律;研究建立的AFLP分子标记体系可以用于梨黑星病的抗性基因标记,并初步找到了与梨黑星病抗性基因相关的分子标记。
