Cardiac hypertrophy in polycythemia vera:A case report and review of literature

BACKGROUND The combination of polycythemia vera(PV)with pathological cardiac hypertro-phy is uncommon.In this study,we describe a case of PV accompanied by pa-thological cardiac hypertrophy.It is hypothesized that the pronounced cardiac hypertrophy in this patient has a strong connection with PV.CASE SUMMARY In 2021,a 34-year-old Chinese man experienced chest constriction,shortness of breath,and palpitations during vigorous activity.Each episode lasted several minutes and resolved spontaneously following cessation of vigorous activity.He occasionally experienced syncope and vertigo without a headache.He underwent cardiac magnetic resonance imaging and was diagnosed with“hypertrophic cardiomyopathy(HCM)”.He was discharged after receiving symptomatic treat-ment,which resulted in an improvement.He presented to our department with chest constriction,shortness of breath,and respiratory distress for one month while climbing to the second floor in 2023.His blood pressure was 180/100 mmHg at the time of admittance,and he was receiving antihypertensive treat-ment.He had a history of PV for 2 years without treatment.Symptomatic treat-ment was implemented concurrently with the administration of hydroxyurea upon admission.Good blood pressure control was observed during the long-term follow-up,and echocardiography did not reveal any progression of myocardial hypertrophy.CONCLUSION Clinicians managing PV patients should remain highly vigilant regarding the risks of thrombosis and cardio-vascular complications,particularly in those with refractory hypertension.

Evaluation of Hemoglobin and Serum Erythropoietin Levels in Patients with Polycythemia Vera and Secondary Polycythemia

Objective: This study compares hemoglobin and erythropoietin levels in patients with polycythemia vera and secondary polycythemia. Study Design: A retrospective cross-sectional study evaluating the serum erythropoietin and hemoglobin levels in patients with polycythemia vera and secondary polycythemia. This study was performed simultaneously in Texas state of the U.S. and Fars Province in Iran. Methods: Hemoglobin, hematocrit and erythropoietin test results were collected from patients aged 19 to 75 years who were diagnosed with polycythemia vera and secondary polycythemia. Patients records with history of thrombocythemia, congestive heart failure, dyspnea, anemia and pregnant woman were excluded from study. Patients in each decade of life were examined in separate groups, so that changes in hemoglobin related to aging did not affect the research results. Results: 75% of the patients were men, and 25% were women. A total of 1580 patients were analyzed in this study. 57.3% of patients in UTMB and 38.8 patients in Iran have hemoglobin level above 17 mg/dl. 74% of patients in UTMB and 88% of patients in Iran have erythropoietin below 10 IU/mL. Polycythemia in UTMB was more common in people over 50 and in Iran in patients under 50 years old. The serum hemoglobin and erythropoietin levels in patients with polycythemia vera were not significantly different in compare to secondary polycythemia patients. Data showed that there were 84 polycythemia patients per 100,000 people. The results of this study in UTMB and Iran showed that 4.5% and 7%, respectively, of patients with polycythemia had a positive JAK2 test. Conclusion: Low erythropoietin levels may not be helpful in differentiating polycythemia vera from secondary polycythemia. .

Does Ruxolitinib, in Comparison to Best Available Therapy (BAT), Improve Pruritis Symptoms in Patients with Polycythemia Vera?

Polycythemia vera manifests as a myeloproliferative neoplasm associated with diverse symptoms, including aquagenic pruritis. This systematic review addresses the pressing need to enhance the understanding of the disease’s symptomatology and optimize treatment strategies for improved patient outcomes. The rarity and low prevalence of polycythemia vera underscore the importance of this investigation, as existing standard of care involves a multifaceted approach and significant healthcare costs. Despite advancement in therapeutic options, persistent symptoms and resistance to first-line treatments pose challenges. Ruxolitinib has emerged as a promising intervention, demonstrating clinically significant improvement for patients. This systematic review appraises three randomized controlled trials, shedding light on the efficacy of ruxolitinib and its potential to ameliorate pruritis symptoms in symptomatic patients.

Micropropagation of Pistacia vera 'Kerman'

[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ＇Kerman＇,tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result]Mediums of 1/2DKW and 1/2DKW＋3.00 mg/L of 6-BA ＋0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio; Proliferation coefficient was 3.6 on the medium of 1/2DKW ＋4.00 mg/L of 6-BA ＋ 0.05 mg/L of IBA; Rooting rate was up to 75% on the medium of 1/2DKW ＋ 5.00 mg/L of IBA ＋ 1.00 mg/L of NAA; The suitable transplantation matrix was 3/4 sand ＋ 1/4 vermiculite,and survival rate of plant with root was above 79%. [Conclusion]The pistachio （Kerman） tissue culture technology system was established.

Anatomy, Histochemistry and Phytochemistry of Leaves in Aloe vera var. chinensis

A multidisciplinary approach-anatomy, histochemistry and phytochemistry-was used to investigate the leaf structure, the content and the storage location of barbaloin in the leaves of Aloe vera L. var. chinensis (Haw.) Berg. Xeromorphic characteristics including secondary thickened epidermal cell walls, thicker cuticle, ambiguous differentiation of spongy and palisade tissues in the chlorenchyma, and well-developed aquiferous tissue could be seen in the leaves. Several large parenchymatous cells were observed at the phloem pole of the first ring of vascular bundles. The secondary ring of vascular bundles in the leaf base and the stomata, which are surrounded by five cells, have some classification significance in this species. The density of vascular bundles, the content of barbaloin and the intensity of histochemical reaction differed among leaf numbers Ll (annual leaf), L2 (biennial leaf), L3 (triennial leaf) and L4 (quadrennial leaf), and in different parts of the leaf. These three factors were highest in the youngest leaf, Ll, and top parts of all the leaves and lowest in the basal parts and the oldest leaf, L4. The density of vascular bundles had a positive correlation to the content of barbaloin. The histochemical results revealed that the small sheath cells that surrounded the bundles might be the location of barbaloin synthesis and the large parenchymatous cells beneath the sheath might be the storage places of this metabolite.

Study on Biological Characteristics of the Pathogen of Pistacia vera Leaf Brown Spot

[Objective] The paper was to study the occurrence rule of Pistacia vera leaf brown spot and the biological characteristics of its pathogen.[Method] The occurrence rule of P.vera leaf brown spot was investigated in field.A fungus was isolated and purified from the diseased tissue of P.vera,its cultural trait and morphological characteristics were observed in indoor experiment,and the classification status was identified according to its morphology.The effects of different temperatures on mycelial growth of the pathogen,as well as the effects of different temperatures and humidity on spore germination of the pathogen were also studied.[Result] P.vera leaf brown spot was a fungal disease,which mainly infected the leaves and fruits of P.vera,causing leaf spot and blight.The disease had serious incidence in introduction place of P.vera in China.The fungal disease was identified to be Alternaria alternaria,the appropriate temperature for mycelial growth was 20-25 ℃,the appropriate temperature for spore germination was 25-30 ℃,and the appropriate relative humidity was over 95%.[Conclusion] Late spring and early summer,as well as late summer and early autumn are two peak periods for incidence of the disease,continuous rain in summer is the decisive condition for the infection and outbreak of P.vera leaf brown spot.

Determination of Barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller by HPLC

A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents of barbaloin and their environments in which they were growing was decided. A Hypersil ODS column (4.6 mm×200 mm, 5 μm)was used with a mobile phase of methanol-water (40:60, containing 0.1% acetic acid), the flow rate being 1.0 mL·min -1, detection wavelength at 359 nm, and the column temperature being 30℃. The linear range of barbaloin was between 0.0726 and 0.726 μg with a correlation coefficient of 0.9998 and the regression equation being Y=1.9202×10 6X-1801.9. Barbaloin was stable in methanol in 48 h and the instrument precision was 1.2% while the method precision was 4.9%. The contents of barbaloin of 12 samples ranged from 6.160 to 319.1 μg·g -1. The method developed was fast and simple with good reproducibility. There was high correlation between the contents of barbaloin and their growing environments.

基于蒙特卡罗程序JMCT模拟计算堆芯物理基准题VERA

JMCT是基于蒙特卡罗方法的中子输运程序,具有几何建模精细、截面数据准确、物理过程真实等特点,计算结果具有更高的精度。通过开发临界硼浓度搜索、输运-热工-燃耗耦合功能模块,使JMCT具备了堆芯物理计算功能。本文利用JMCT程序对CASL项目提出的堆芯物理基准题库VERA进行模拟,获得了k_(eff)、控制棒价值、反应性系数等启动物理参数以及硼降曲线等堆芯运行参数。JMCT计算结果与蒙特卡罗程序KENO-Ⅵ以及MC21进行了对比,结果符合良好,证明了JMCT具有堆芯物理计算能力,并具有较高的精度。

基于Open Vera的IIC总线接口功能验证平台的搭建

文章讨论了基于Open Vera的功能验证平台的搭建方法,重点研究了如何挖掘Open Vera所支持的各种新技术和新功能,并与传统验证方法所搭建的验证平台进行了性能比较;表明该硬件验证语言的优势,对验证IP(VIP:Verification IP)的编写有一定的借鉴意义。

一种基于Vera的集成电路建模验证方法

随着数字逻辑设计越来越复杂,验证的难度也越来越大。根据一款以太网交换芯片的项目完成所总结的经验,提出了一种基于Vera的电路行为级建模的验证方法。实验结果表明,这种验证方法可以方便地进行RTL和参考模型的联合仿真,并能最大限度地提高验证覆盖率,有效地减少验证工作量和缩短验证时间。

用NECP-X程序计算与分析VERA 9^(#)基准题

利用自主开发的确定论数值反应堆物理计算程序NECP-X对VERA(virtual environment for reactor application)9#基准题进行全堆芯精细建模计算,显式描述了堆芯格架、管座、端塞、压紧弹簧及气腔等结构,计算得到了整个寿期中的临界硼质量分数,给出了3维全堆芯棒功率分布、燃料有效温度分布及慢化剂温度分布,并将计算结果与实测值进行了比较。结果表明,整个寿期中临界硼质量分数的计算值与实测值的偏差小于2.5×10^(-5),计算精度较好;在整个寿期内,随着燃耗的加深,堆芯功率分布逐渐展平,燃料有效温度及慢化剂温度的分布逐渐变得均匀,符合物理规律及预期。

长期盐胁迫对库拉索芦荟(Aloe vera)生长和汁液理化性质的影响

在温室中砂培条件下研究了7叶龄库拉索芦荟(Aloe vera)以含0、50、100、200和400 mmol L-1NaCl的Hoagland营养液浇灌120 d的生长响应及其全叶原汁的理化性质。结果表明:200 mmol L-1NaCl处理显著抑制芦荟生长,使叶片长度、宽度、厚度和重量显著减小,含水量和叶绿素含量降低,干枯死亡的叶片数显著增多,根系周界和根系密集区显著缩小,单株干重降至对照的65.02%,单株鲜重只及对照的38.32%,至开花期花葶低矮、细弱,开花株数明显减少;100 mmol L-1NaCl处理对库拉索芦荟生长的抑制作用显著减轻,且可正常开花,花期未见缩短,但至处理结束时新叶的长度,处理结束时单叶的厚度和鲜重、根系密集区范围以及叶片和全株的鲜重、根系的干重和鲜重均显著小于对照,而50 mmol L-1NaCl处理与对照无显著性差异。盐胁迫对库拉索芦荟全叶原汁出汁率及理化性质的影响与对生长开花的影响相似,其中501、00 mmol L-1NaCl处理间多数指标值无显著性差异,与对照相比多数指标值处于有益水平。综合评判:库拉索芦荟具有咸水微咸水灌溉栽培的潜势。

活性炭在库拉索芦荟(Aloe vera)的组织培养中的应用

探索了库拉索芦荟组织培养中活性炭(AC)的添加量与植物激素添加量的关系以及对库拉索芦荟外植体出芽率的影响。结果表明,在MS固体培养基中,活性炭对植物激素的吸附量不能简单地套用MS液体培养基中的吸附量,活性炭的吸附作用情况较复杂;库拉索芦荟外植体出芽的较好培养基为MS+6 BA3mg/L+NAA0 2mg/L+AC0 2%和MS+6 BA5mg/L+NAA0 2mg/L+AC0 4%。

Effects of Aloe vera and sucralfate on gastric microcirculatory changes,cytokine levels and gastric ulcer healing in rats

AIM： To compare the effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing. METHODS： Male Spraque-Dawley rats （n=48） were divided into four groups. Group 1 served as control group, group 2 as gastric ulcer group without treatment, groups 3 and 4 as gastric ulcer treatment groups with sucralfate and Aloe vera. The rats from each group were divided into 2 subgroups for study of leukocyte adherence, TNF-α and IL-10 levels and gastric ulcer healing on days 1 and 8 after induction of gastric ulcer by 20% acetic acid. RESULTS： On day 1 after induction of gastric ulcer, the leukocyte adherence in postcapillary venule was significantly （P〈 0.05） increased in the ulcer groups when compared to the control group. The level of TNF-α was elevated and the level of IL-10 was reduced. In the ulcer groups treated with sucralfate and Aloe vera, leukocyte adherence was reduced in postcapillary venule. The level of IL-10 was elevated, but the level of TNF-a had no significant difference. On day 8, the leukocyte adherence in postcapillary venule and the level of TNF-α were still increased and the level of IL-10 was reduced in the ulcer group without treatment. The ulcer treated with sucralfate and Aloe vera had lower leukocyte adherence in postcapillary venule and TNF-α level. The level of IL-10 was still elevated compared to the ulcer group without treatment. Furthermore, histopathological examination of stomach on days 1 and 8 after induction of gastric ulcer showed that gastric tissue was damaged with inflammation. In the ulcer groups treated with sucralfate and Aloe vera on days 1 and 8, gastric inflammation was reduced, epithelial cell proliferation was enhanced and gastric glands became elongated. The ulcer sizes were also reduced compared to the ulcer group without treatment. CONCLUSION： Administration of 20% acetic acid can induce gastric inflammation, increase leukocyte adherence in postcapillary venule and TNF-α level and reduce IL-10 level. Aloe vera treatment can reduce leukocyte adherence and TNF-αlevel, elevate IL-10 level and promote gastric ulcer healing.

断言验证技术在VERA中的应用与研究

本文首先介绍断言验证技术,对断言的特点及使用方法进行详细介绍,然后讲解OVA的语法结构,以及如何在测试平台中加入断言文件,并以交通灯为例,进行断言分析。

影响库拉索芦荟(Aloe vera L.)愈伤组织诱导的因素

比较了不同培养基对库拉索芦荟愈伤组织的诱导效果,其中B5+6 BA2.0mg/L+2,4 D2.0mg/L+Vc5.0mg/L培养基出愈率较高,愈伤组织褐化较轻.探讨了抑制库拉索芦荟愈伤组织褐化的方法.

基于VERA基准题的cosRMC程序验证

为了验证反应堆物理软件和方法的计算能力,美国CASL(Consortium for Advanced Simulation of LWRs)项目提出了VERA(Virtual Environment for Reactor Application)堆芯物理基准题。该基准题以Watts Bar初始堆芯为模型,涵盖从二维单栅元到三维全堆芯的燃耗及换料的十个基准问题。针对VERA基准题模型,利用COSINE软件包中的反应堆蒙特卡罗分析程序cosRMC进行临界计算,得到了有效增殖因子、组件功率分布、控制棒微积分价值和反应性系数等结果。通过与基准题中提供的KENO结果对比,两种蒙特卡罗程序的计算结果吻合良好。这表明cosRMC程序具有从组件到堆芯的计算能力,其临界计算精度基本与KENO程序相当。

Low-temperature green synthesis of boron carbide using aloe vera

The unique structural and physical properties of boron carbide, which make it suitable for a wide range of applications,demands the development of low-cost and green synthesis method. In the present work, the commonly available leaves of aloe vera are hydrothermally treated to form the carbon precursor for the synthesis of boron carbide. The morphological characterization reveals the porous nature of the precursor turning into a tubular structure upon boron carbide formation.The structural characterization by x-ray diffraction and other spectroscopic techniques such as Fourier transform infrared,Raman, photoluminescence and uv-visible near-infrared spectroscopy confirm the formation of boron carbide. The thermogravimetric analysis of the sample is found to exhibit good thermal stability above 500 °C. When the sample is annealed to 600 °C, boron carbide with phase purity is obtained, which is confirmed through XRD and FTIR analyses. The optical emission properties of the sample are studied through CIE plot and power spectrum. Compared with other natural precursors for boron carbide, the aloe vera is found to give a good yield above 50%.

一种基于Open Vera实现的L2 cache验证平台

随着设计复杂度的不断增加和设计规模的不断增大,传统的验证工具已难以适应当前功能验证的要求.Vera为验证增添了强大的语言能力.使用Vera建立验证平台,它独有的特性能够隐藏设计中的复杂性,从而使testbench的编写更见简洁.通过使用动态的激励驱动,实现了实际工作中的状态和全部极端的条件,从而发现设计中存在的瑕疵.使用Open Vera验证语言构建了"龙腾R2"L2cache验证平台,成功完成了"龙腾R2"的验证工作.

Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells

Objective: To investigate the in vitro anticancer effect of Aloe vera(A. vera) and Calligonum comosum(C. comosum) extracts against hepatocellular carcinoma(HepG 2) cells. Methods: Hep G2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG 2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic(Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG 2 cells were observed using light microscopy.Results: The IC50 values of A. vera and C. comosum extracts were(10.45 ± 0.31) and(9.60 ± 0.01) μg/mL respectively. The extracts separately increased cytotoxicity against HepG 2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions.Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis.