Vibramycin inhibits the expression of MMP-2 and the invasiveness of PC-3 cells in vitro

Objective To study the inhibition of vibramycin on the expression of matrix metalloproteinase-2 (MMP-2) and the invasiveness of androgen-independent prostatic carcinoma cell line PC - 3 in vitro. Methods Immunohistochemistry stain and transwell chamber were used to investigate the expression of MMP-2 in different concentration of vibramycin treated PC-3 cells and the invasive ability of different concentration of vibramycin treated PC-3 cell. Results The positive rate of MMP-2 inJune 2003 Vol12 No2 PC-3 cells was decreased at a concentration of 5 mg/L of vibramycin and decreased dramatically at the concentration of 10 mg/L. The cells moved throuth the membrane was(82. 0 ± 4.6)/field in the control group, while decreased to(26.1 ±3.6),(7.2 ±2.2) and(3.3± 0.7)/field in 5,10 and 20 mg/L vibramycin treated PC-3 cell respectively. Conclusion Vibramycin can inhibit the invasiveness and metastatasis of PC-3 cells, the mechanism of which is related to the inhibition of MMP-2 in PC-3 cell.10refs.

联合用药治疗女性生殖道支原体感染82例临床分析

目的 探讨生殖道支原体感染的有效治疗方法。方法 把152例病人随机分为观察组82例和对照 组70例,观察组给予强力霉素口服和阴道局部应用阿希米联合用药治疗,对照组仅给予强力霉素口服治疗。结 果 观察组总有效率为91.46％;对照组总有效率为78.57％。两组比较差异有显著性(P<0.05)。而白带增多、外阴 瘙痒等临床症状的消失率观察组(94.7％)亦显著高于对照组(75％),P<0.05。结论 强力霉素与阴道局部应用阿 希米联合用药治疗生殖道支原体感染是安全、有效的。

强力霉素抑制PC-3细胞基质金属蛋白酶-2表达及侵袭的体外研究

目的 探讨强力霉素对雄激素非依赖型前列腺癌PC 3细胞基质金属蛋白酶 2 (MMP 2 )表达的抑制及与体外侵袭能力的关系。 方法 采用免疫组织化学方法和Transwell小室法构建体外侵袭模型 ,研究不同浓度强力霉素对PC 3细胞MMP 2表达及其体外侵袭转移能力的影响。 结果 免疫组化方法检测结果显示 5mg/L强力霉素可以抑制PC 3细胞中MMP 2蛋白的表达 ,1 0mg/L时作用更明显。体外侵袭试验结果显示对照组每 2 0 0倍视野穿过的细胞数平均为 (82 .0± 4 .6)个 ,而 5、1 0和 2 0mg/L强力霉素作用 2d后 ,每 2 0 0倍视野穿过的细胞数分别为 (2 6 .1± 3 .6)、(7.2± 2 .2 )和 (3 .3± 0 .7)个 ,与对照组相比差别均有显著性意义 ,P <0 .0 1。 结论 强力霉素抑制PC 3侵袭及转移的作用与其抑制MMP