Viburnum dilatatum(jiami in Chinese),belonging to the Caprifollaceae family,is widely distributed in Japan and China.Phytochemical investigations of Viburnum dilatatum(V.dilatatum)have resulted in the isolation of tri...Viburnum dilatatum(jiami in Chinese),belonging to the Caprifollaceae family,is widely distributed in Japan and China.Phytochemical investigations of Viburnum dilatatum(V.dilatatum)have resulted in the isolation of triterpenoids,phenolic glycosides essential oil,norisoprenoids,etc.Research results have shown that the chemical constituents of V.dilatatum possess various pharmacological activities,including antihyperglycemic,antioxidant activity and antiulcer effects.This study reviewed the chemical constituents and pharmacological activities of V.dilatatum to provide practical and useful information for further research and development of this plant.展开更多
[Objective] This study aimed to study the pharmacognostical properties of medicinal parts of stems and leaves of Viburnum Sargenti Koehne to provide refer- ence for its identification and application. [Method] Methods...[Objective] This study aimed to study the pharmacognostical properties of medicinal parts of stems and leaves of Viburnum Sargenti Koehne to provide refer- ence for its identification and application. [Method] Methods of characters and micro- scopic identification were used. [Result] Characters, transaction and microscopic characteristics of stems and leaves of V. sargenti Koehne were analyzed systemati- cally. [Conclusion] The experimental results can provide reference for the pharma- cognostical identification, further exploitation and utilization of stems and leaves of V. Sargenti Koehne.展开更多
Distinctness,uniformity and stability(DUS)Test Guidelines for new varieties of Viburnum L.were developed according to the principles and requirements of the International Union for the Protection of New Varieties of P...Distinctness,uniformity and stability(DUS)Test Guidelines for new varieties of Viburnum L.were developed according to the principles and requirements of the International Union for the Protection of New Varieties of Plants(UPOV)and China.The development processes,including selection and determination of testing characteristics,determination of expression states of characteristics,selection of example varieties,determination of the order of characteristics,and assessment of distinctness,uniformity and stability,are described in this paper.There were 54 testing characteristics:24 qualitative characteristics(QL),15 quantitative characteristics(QN),15 pseudoqualitative characteristics(PQ)and 34 example varieties in the DUS Test Guidelines.Among the 54 testing characteristics,20 were asterisked and 34 were non-asterisked.Seven were selected for variety groupings and 18 were adopted in the Technical Questionnaire.The development of these DUS Test Guidelines will be useful for the application and protection of new Viburnum varieties.The Guidelines will provide technical support for DUS testing of Viburnum and serve as a reference for the development of new varieties in other countries.展开更多
A new stigmasterol type natural product,viburodorol A(1),along with eleven known sterols and terpenoids(2–12),were isolated from the aerial parts of Viburnum odoratissimum.The structure of 1 was elucidated on the bas...A new stigmasterol type natural product,viburodorol A(1),along with eleven known sterols and terpenoids(2–12),were isolated from the aerial parts of Viburnum odoratissimum.The structure of 1 was elucidated on the basis of comprehensive spectroscopic analysis.It’s noteworthy that compound 2,the major constituent of this plant,can signifi-cantly stimulate glucose absorption in insulin resistant HepG2 cells without affecting cell viability.Furthermore,this compound can also restore the glucose absorption in DXMS-induced insulin resistant 3T3-L1 cells.展开更多
Under untreated conditions, the germination rate of Viburnum sargentii seeds is very low. By exploring the relationship between the dynamic changes of physicochemical indicators and endogenous hormones and seed germin...Under untreated conditions, the germination rate of Viburnum sargentii seeds is very low. By exploring the relationship between the dynamic changes of physicochemical indicators and endogenous hormones and seed germination during the dormancy and germination of Viburnum sargentii seeds, the mechanism of seed germination of Viburnum sargentii was determined, which provided a theoretical basis for its extensive promotion and development. The contents of soluble sugar and soluble starch were determined by anthrone colorimetry. The soluble protein was determined by Coomassie brilliant blue G-250 staining. The activity of polyphenol oxidase (PPO) was determined by NBT reduction method. The peroxidase (POD) was determined by guaiacol method. And the endogenous hormones ZA, IAA, ABA, GA3 were determined by liquid chromatography. Results show: 1) Under natural conditions, the seeds of Viburnum sargentii are difficult to germinate. 2) Under sand storage for 8 months, the germination rate of untreated seeds was 33%. Puncture and peeling treatment could significantly increase the germination rate, and the germination rate of seeds treated with puncture was 92%, and that treated with peel was 98%. 3) Seed germination was accompanied by the decrease in macromolecular substances such as soluble sugar, soluble starch, and soluble protein. 4) The dynamic changes of hormones during seed germination conform to the hypothesis of “three factors”. 5) The treatment of puncture and peeling increased the content of endogenous hormones promoting germination, decreased the endogenous hormones inhibiting seed germination, and increased the ratio of (IAA + GA + ZR)/ABA or GA/ABA. The seeds of Viburnum sargentii have obvious dormancy characteristics. Under the condition of sand storage, both pricking and peeling treatment can effectively promote the process of breaking dormancy, and the effect of peeling treatment is better.展开更多
Viburnum odoratissimum,a landscape plant,is rich in vibsane-type diterpenes,triterpenes,monoterpenoids,sesquiterpenoids,coumarins,and lignans and so on.In the present work,network analysis was carried out to predict t...Viburnum odoratissimum,a landscape plant,is rich in vibsane-type diterpenes,triterpenes,monoterpenoids,sesquiterpenoids,coumarins,and lignans and so on.In the present work,network analysis was carried out to predict the potential targets of V.odoratissimum and the treatment of related diseases.13 main target proteins were predicted,as well as the diseases which might interact with the compounds from V.odoratissimum.The results showed that vibsane-type diterpenes and triterpenes had the potential to treat neoplasm-related and inflammation-related diseases,and the key target proteins were MDM2,NOS,AR,DNMT1,NR3C1,RARA and PTPN1.Among them,AR,NOS,DNMT1,NR3C1,DNMT1 and PTPN1 were also the crucial targets for triterpenes in the treatment of diabetes mellitus.Besides,ALOX15,PIPN1,TLR9,MIT,DNMT1,RAC1,RTGS2,RARA and ACHE were the potential targets for coumarins and lignan in the treatment of neoplasm-related diseases.Furthermore,ESR1,AR,NOS2,NR3C1 were the primary targets for monoterpenoids and sesquiterpenoids because of their potential neuroprotective effects.It is noteworthy that the study of the potential targets and related diseases can provide new insights for further development of V.odoratissimum.展开更多
Viburnum awabuki(V.awabuki),belonging to the Viburnum genus,is an ideal tree species for landscaping.Phytochemical investigations of the V.awabuki have resulted in the isolation and identification of coumarins,lignans...Viburnum awabuki(V.awabuki),belonging to the Viburnum genus,is an ideal tree species for landscaping.Phytochemical investigations of the V.awabuki have resulted in the isolation and identification of coumarins,lignans,sesquiterpenes,diterpenes and triterpenes.It has been reported that the extracts of V.awabuki have multiple pharmacological activities,including anti-tumor,anti-oxidant,plant growth inhibitory and neurite outgrowth-promoting activities.The research progress on the chemical constituents of V.awabuki and pharmacological activities were summarized in this review.展开更多
Viburnum tinus L.is a shrub native to the Mediterranean basin.Because of its sedative and antispasmodic activities,it is often used as a folk medicine in Europe.Phytochemical researches have shown that numerous chemic...Viburnum tinus L.is a shrub native to the Mediterranean basin.Because of its sedative and antispasmodic activities,it is often used as a folk medicine in Europe.Phytochemical researches have shown that numerous chemical components,including iridoid glycosides,diterpenes,triterpenes,coumarin,flavonoids and anthocyanidins have been isolated from Viburnum tinus L..This study reviewed the chemical constituents and pharmacological activities of Viburnum tinus L.to provide a reference for further researches.展开更多
Viburnum odoratissimum belongs to viburnum genus of the Caprifoliaceae family.Previous studies reported many chemical constituents in this plant,including monoterpenoids,sesquiterpenoids,vibsane-type diterpenes,triter...Viburnum odoratissimum belongs to viburnum genus of the Caprifoliaceae family.Previous studies reported many chemical constituents in this plant,including monoterpenoids,sesquiterpenoids,vibsane-type diterpenes,triterpenes,coumarins,and lignans,and various pharmacological bioactivities the extracts of V.odoratissimum such as anti-tumor,neuroprotective,antiinflammatory,ichthyism and anti-oxidant activity.Besides,the total synthesis of vibsane-type diterpenes and their derivatives have attracted considerable attention because of their wide-ranging anti-tumor activity.This present study not only systematically summarizes the compounds isolated from V.odoratissimum,and the structure-activity relationship of these compounds.展开更多
Viburnum suspensum Lindley,belonging to genus Viburnum of Caprifoliaceae family,is distributed in subtropical area of Japan.Phytochemical reports showed that the main chemical constituents of V.suspensum are irldold g...Viburnum suspensum Lindley,belonging to genus Viburnum of Caprifoliaceae family,is distributed in subtropical area of Japan.Phytochemical reports showed that the main chemical constituents of V.suspensum are irldold glycosldes,labdane-type diglucosides,vibsane-type diterpenoids and oleanane triterpenoids.These compounds were found to possess several biological activities and have attracted great attention of researchers worldwide.This study reviews the chemical constituents and biological activities of V.suspensum,aiming to provide insight for further research on this plant.展开更多
Viburnum opulus,belonging to Viburnum of Caprifoliaceae family,is commonly used as a Chinese herbal medicine.Many chemical constituents have been isolated from V.opulus,including flavonoids,flavone glycosides,volatile...Viburnum opulus,belonging to Viburnum of Caprifoliaceae family,is commonly used as a Chinese herbal medicine.Many chemical constituents have been isolated from V.opulus,including flavonoids,flavone glycosides,volatile oils and terpenoids.It has antioxidant,antibacterial,antispasmodic,and anticancer effects.V.opulus,with a variety of chemical constituents and remarkable activities,has attracted wide attention.Hence,this report is presented as a review centered on the chemical constituents and pharmacological activities of V.opulus,in order to provide reference for further research and development of Viburnum opulus.展开更多
Viburnum lantana L.(Caprifoliaceae)is a shrub up to 3-5 m tall,with red or black drupes.Researchers have studied the chemical composition of different parts of the plant and found many compounds with novel structures,...Viburnum lantana L.(Caprifoliaceae)is a shrub up to 3-5 m tall,with red or black drupes.Researchers have studied the chemical composition of different parts of the plant and found many compounds with novel structures,including chalcone glycosides,iridoid glycosides,and some terpenoids.At the same time,it has been reported that the extracts from different parts of V.lantana have a variety of biological activities,including antioxidant,antimicrobial,and AChE Inhibitory activities.This study reviewed the chemical components and bioactivities of the V.lantana reported in recent years.展开更多
Five new terpenoids,including two vibsane-type diterpenoids(1,2)and three iridoid allosides(3-5),together with eight known ones,were isolated from the leaves and twigs of Viburnum odoratissimum var.sessiliflorum.Their...Five new terpenoids,including two vibsane-type diterpenoids(1,2)and three iridoid allosides(3-5),together with eight known ones,were isolated from the leaves and twigs of Viburnum odoratissimum var.sessiliflorum.Their planar structures and relative configurations were determined by spectroscopic methods,especially 2D NMR techniques.The sugar moieties of the iridoids were confirmed asβ-D-allose by GC analysis after acid hydrolysis and acetylation.The absolute configurations of neovibsanin Q(1)and dehydrovibsanol B(2)were determined by quantum chemical calculation of their theoretical electronic circular dichroism(ECD)spectra and Rh2(OCOCF_(3))_(4)-induced ECD analysis.The anti-inflammatory activities of compounds 1,3,4,and 5 were evaluated using an LPS-induced RA W264.7 cell model.Compounds 3 suppressed the release of NO in a dose-dependent manner,with an IC_(50) value of 55.64μmol·L^(-1).The cytotoxicities of compounds 1-5 on HCT-116 cells were assessed and the results showed that compounds 2 and 3 exhibited moderate inhibitory activities with IC_(50) values of 13.8 and 12.3μmol·L^(-1),respectively.展开更多
[目的]探究该物种扦插生根机理,便于品种的快速繁殖及应用推广。[方法]以不同扦插基质类型、激素类型及激素水平组合测定地中海荚蒾扦插后0、5、10、15和20 d 5个时期的生根情况及不同营养物质、相关酶活性和内源激素等生理指标的变化...[目的]探究该物种扦插生根机理,便于品种的快速繁殖及应用推广。[方法]以不同扦插基质类型、激素类型及激素水平组合测定地中海荚蒾扦插后0、5、10、15和20 d 5个时期的生根情况及不同营养物质、相关酶活性和内源激素等生理指标的变化趋势。[结果]扦插基质类型、外源激素类型及浓度对荚蒾扦插生根率、生根数及根长产生了极显著差异,蛭石、珍珠岩混合基质与NAA+IBA(500 mg/L)激素组合下地中海荚蒾的生根效果最好,其生根率、生根数和根长最高。可溶性糖、可溶性蛋白含量均呈上升—下降—上升的趋势,均在插后第5天达到峰值。过氧化物酶(POD)活性呈现上升—下降—再上升的趋势,多酚氧化酶(PPO)和吲哚乙酸氧化酶(IAAO)活性呈先上升后下降的变化趋势。4种内源激素(IAA、ABA、GA_(3)、ZR)含量变化较为复杂,呈波浪型变化。[结论]蛭石、珍珠岩混合基质与NAA+IBA(500 mg/L)激素组合的处理方式适用于地中海荚蒾的扦插。该研究开展的通过外源施加物质的方法提高扦插成活率提供了理论基础,也为地中海荚蒾南种北繁起到了促进作用。展开更多
文摘Viburnum dilatatum(jiami in Chinese),belonging to the Caprifollaceae family,is widely distributed in Japan and China.Phytochemical investigations of Viburnum dilatatum(V.dilatatum)have resulted in the isolation of triterpenoids,phenolic glycosides essential oil,norisoprenoids,etc.Research results have shown that the chemical constituents of V.dilatatum possess various pharmacological activities,including antihyperglycemic,antioxidant activity and antiulcer effects.This study reviewed the chemical constituents and pharmacological activities of V.dilatatum to provide practical and useful information for further research and development of this plant.
基金Supported by National Natural Science Foundation of China(31100401)~~
文摘[Objective] This study aimed to study the pharmacognostical properties of medicinal parts of stems and leaves of Viburnum Sargenti Koehne to provide refer- ence for its identification and application. [Method] Methods of characters and micro- scopic identification were used. [Result] Characters, transaction and microscopic characteristics of stems and leaves of V. sargenti Koehne were analyzed systemati- cally. [Conclusion] The experimental results can provide reference for the pharma- cognostical identification, further exploitation and utilization of stems and leaves of V. Sargenti Koehne.
基金supported by Forestry Science&Technology Innovation Project of Shandong Province(LYCX01-2018-03)Fund for the Development of Guidelines for the Conduct of Tests for Distinctness,Uniformity and Stability(201519)
文摘Distinctness,uniformity and stability(DUS)Test Guidelines for new varieties of Viburnum L.were developed according to the principles and requirements of the International Union for the Protection of New Varieties of Plants(UPOV)and China.The development processes,including selection and determination of testing characteristics,determination of expression states of characteristics,selection of example varieties,determination of the order of characteristics,and assessment of distinctness,uniformity and stability,are described in this paper.There were 54 testing characteristics:24 qualitative characteristics(QL),15 quantitative characteristics(QN),15 pseudoqualitative characteristics(PQ)and 34 example varieties in the DUS Test Guidelines.Among the 54 testing characteristics,20 were asterisked and 34 were non-asterisked.Seven were selected for variety groupings and 18 were adopted in the Technical Questionnaire.The development of these DUS Test Guidelines will be useful for the application and protection of new Viburnum varieties.The Guidelines will provide technical support for DUS testing of Viburnum and serve as a reference for the development of new varieties in other countries.
基金the foundations from NSFC(20972167)the Young Academic Leader Raising Foundation of Yunnan Province(No.2009CI073)。
文摘A new stigmasterol type natural product,viburodorol A(1),along with eleven known sterols and terpenoids(2–12),were isolated from the aerial parts of Viburnum odoratissimum.The structure of 1 was elucidated on the basis of comprehensive spectroscopic analysis.It’s noteworthy that compound 2,the major constituent of this plant,can signifi-cantly stimulate glucose absorption in insulin resistant HepG2 cells without affecting cell viability.Furthermore,this compound can also restore the glucose absorption in DXMS-induced insulin resistant 3T3-L1 cells.
文摘Under untreated conditions, the germination rate of Viburnum sargentii seeds is very low. By exploring the relationship between the dynamic changes of physicochemical indicators and endogenous hormones and seed germination during the dormancy and germination of Viburnum sargentii seeds, the mechanism of seed germination of Viburnum sargentii was determined, which provided a theoretical basis for its extensive promotion and development. The contents of soluble sugar and soluble starch were determined by anthrone colorimetry. The soluble protein was determined by Coomassie brilliant blue G-250 staining. The activity of polyphenol oxidase (PPO) was determined by NBT reduction method. The peroxidase (POD) was determined by guaiacol method. And the endogenous hormones ZA, IAA, ABA, GA3 were determined by liquid chromatography. Results show: 1) Under natural conditions, the seeds of Viburnum sargentii are difficult to germinate. 2) Under sand storage for 8 months, the germination rate of untreated seeds was 33%. Puncture and peeling treatment could significantly increase the germination rate, and the germination rate of seeds treated with puncture was 92%, and that treated with peel was 98%. 3) Seed germination was accompanied by the decrease in macromolecular substances such as soluble sugar, soluble starch, and soluble protein. 4) The dynamic changes of hormones during seed germination conform to the hypothesis of “three factors”. 5) The treatment of puncture and peeling increased the content of endogenous hormones promoting germination, decreased the endogenous hormones inhibiting seed germination, and increased the ratio of (IAA + GA + ZR)/ABA or GA/ABA. The seeds of Viburnum sargentii have obvious dormancy characteristics. Under the condition of sand storage, both pricking and peeling treatment can effectively promote the process of breaking dormancy, and the effect of peeling treatment is better.
基金This work was supported by National Natural Science Foundation of China[Grant No.81703385].
文摘Viburnum odoratissimum,a landscape plant,is rich in vibsane-type diterpenes,triterpenes,monoterpenoids,sesquiterpenoids,coumarins,and lignans and so on.In the present work,network analysis was carried out to predict the potential targets of V.odoratissimum and the treatment of related diseases.13 main target proteins were predicted,as well as the diseases which might interact with the compounds from V.odoratissimum.The results showed that vibsane-type diterpenes and triterpenes had the potential to treat neoplasm-related and inflammation-related diseases,and the key target proteins were MDM2,NOS,AR,DNMT1,NR3C1,RARA and PTPN1.Among them,AR,NOS,DNMT1,NR3C1,DNMT1 and PTPN1 were also the crucial targets for triterpenes in the treatment of diabetes mellitus.Besides,ALOX15,PIPN1,TLR9,MIT,DNMT1,RAC1,RTGS2,RARA and ACHE were the potential targets for coumarins and lignan in the treatment of neoplasm-related diseases.Furthermore,ESR1,AR,NOS2,NR3C1 were the primary targets for monoterpenoids and sesquiterpenoids because of their potential neuroprotective effects.It is noteworthy that the study of the potential targets and related diseases can provide new insights for further development of V.odoratissimum.
文摘Viburnum awabuki(V.awabuki),belonging to the Viburnum genus,is an ideal tree species for landscaping.Phytochemical investigations of the V.awabuki have resulted in the isolation and identification of coumarins,lignans,sesquiterpenes,diterpenes and triterpenes.It has been reported that the extracts of V.awabuki have multiple pharmacological activities,including anti-tumor,anti-oxidant,plant growth inhibitory and neurite outgrowth-promoting activities.The research progress on the chemical constituents of V.awabuki and pharmacological activities were summarized in this review.
文摘Viburnum tinus L.is a shrub native to the Mediterranean basin.Because of its sedative and antispasmodic activities,it is often used as a folk medicine in Europe.Phytochemical researches have shown that numerous chemical components,including iridoid glycosides,diterpenes,triterpenes,coumarin,flavonoids and anthocyanidins have been isolated from Viburnum tinus L..This study reviewed the chemical constituents and pharmacological activities of Viburnum tinus L.to provide a reference for further researches.
基金supported by National Natural Science Foundation of China[Grant No.81703385].
文摘Viburnum odoratissimum belongs to viburnum genus of the Caprifoliaceae family.Previous studies reported many chemical constituents in this plant,including monoterpenoids,sesquiterpenoids,vibsane-type diterpenes,triterpenes,coumarins,and lignans,and various pharmacological bioactivities the extracts of V.odoratissimum such as anti-tumor,neuroprotective,antiinflammatory,ichthyism and anti-oxidant activity.Besides,the total synthesis of vibsane-type diterpenes and their derivatives have attracted considerable attention because of their wide-ranging anti-tumor activity.This present study not only systematically summarizes the compounds isolated from V.odoratissimum,and the structure-activity relationship of these compounds.
文摘Viburnum suspensum Lindley,belonging to genus Viburnum of Caprifoliaceae family,is distributed in subtropical area of Japan.Phytochemical reports showed that the main chemical constituents of V.suspensum are irldold glycosldes,labdane-type diglucosides,vibsane-type diterpenoids and oleanane triterpenoids.These compounds were found to possess several biological activities and have attracted great attention of researchers worldwide.This study reviews the chemical constituents and biological activities of V.suspensum,aiming to provide insight for further research on this plant.
文摘Viburnum opulus,belonging to Viburnum of Caprifoliaceae family,is commonly used as a Chinese herbal medicine.Many chemical constituents have been isolated from V.opulus,including flavonoids,flavone glycosides,volatile oils and terpenoids.It has antioxidant,antibacterial,antispasmodic,and anticancer effects.V.opulus,with a variety of chemical constituents and remarkable activities,has attracted wide attention.Hence,this report is presented as a review centered on the chemical constituents and pharmacological activities of V.opulus,in order to provide reference for further research and development of Viburnum opulus.
文摘Viburnum lantana L.(Caprifoliaceae)is a shrub up to 3-5 m tall,with red or black drupes.Researchers have studied the chemical composition of different parts of the plant and found many compounds with novel structures,including chalcone glycosides,iridoid glycosides,and some terpenoids.At the same time,it has been reported that the extracts from different parts of V.lantana have a variety of biological activities,including antioxidant,antimicrobial,and AChE Inhibitory activities.This study reviewed the chemical components and bioactivities of the V.lantana reported in recent years.
基金supported by the National Natural Science Foundation of China(Nos.22077111,22177016,81872756,and 81901678)Department of Education of Guangdong Province(No.2020KZDZX1203)。
文摘Five new terpenoids,including two vibsane-type diterpenoids(1,2)and three iridoid allosides(3-5),together with eight known ones,were isolated from the leaves and twigs of Viburnum odoratissimum var.sessiliflorum.Their planar structures and relative configurations were determined by spectroscopic methods,especially 2D NMR techniques.The sugar moieties of the iridoids were confirmed asβ-D-allose by GC analysis after acid hydrolysis and acetylation.The absolute configurations of neovibsanin Q(1)and dehydrovibsanol B(2)were determined by quantum chemical calculation of their theoretical electronic circular dichroism(ECD)spectra and Rh2(OCOCF_(3))_(4)-induced ECD analysis.The anti-inflammatory activities of compounds 1,3,4,and 5 were evaluated using an LPS-induced RA W264.7 cell model.Compounds 3 suppressed the release of NO in a dose-dependent manner,with an IC_(50) value of 55.64μmol·L^(-1).The cytotoxicities of compounds 1-5 on HCT-116 cells were assessed and the results showed that compounds 2 and 3 exhibited moderate inhibitory activities with IC_(50) values of 13.8 and 12.3μmol·L^(-1),respectively.
文摘[目的]探究该物种扦插生根机理,便于品种的快速繁殖及应用推广。[方法]以不同扦插基质类型、激素类型及激素水平组合测定地中海荚蒾扦插后0、5、10、15和20 d 5个时期的生根情况及不同营养物质、相关酶活性和内源激素等生理指标的变化趋势。[结果]扦插基质类型、外源激素类型及浓度对荚蒾扦插生根率、生根数及根长产生了极显著差异,蛭石、珍珠岩混合基质与NAA+IBA(500 mg/L)激素组合下地中海荚蒾的生根效果最好,其生根率、生根数和根长最高。可溶性糖、可溶性蛋白含量均呈上升—下降—上升的趋势,均在插后第5天达到峰值。过氧化物酶(POD)活性呈现上升—下降—再上升的趋势,多酚氧化酶(PPO)和吲哚乙酸氧化酶(IAAO)活性呈先上升后下降的变化趋势。4种内源激素(IAA、ABA、GA_(3)、ZR)含量变化较为复杂,呈波浪型变化。[结论]蛭石、珍珠岩混合基质与NAA+IBA(500 mg/L)激素组合的处理方式适用于地中海荚蒾的扦插。该研究开展的通过外源施加物质的方法提高扦插成活率提供了理论基础,也为地中海荚蒾南种北繁起到了促进作用。