The effect of functionalized graphene on the growth and development of Vicia faba L.was investigated by analyzing its impact on the composition and diversity of the microbial community in rhizosphere peat soil.Seedlin...The effect of functionalized graphene on the growth and development of Vicia faba L.was investigated by analyzing its impact on the composition and diversity of the microbial community in rhizosphere peat soil.Seedlings of V.faba planted in this peat soil were treated with either distilled water(CK)or 25 mg·L^(−1)(G25)of functionalized graphene solution.Results showed that the height and root length of V.faba seedlings in the G25 group were significantly larger than those in CK group.The microbial com-munity was analyzed by amplifying and sequencing the 16S rRNA gene V_(3)-V_(4) region of bacteria and internal transcribed spacer re-gion of fungi in rhizosphere soil using Illumina MiSeq technology.Alpha and beta diversity analysis indicated that functionalized graphene increased the richness and diversity of bacteria and fungi in the V.faba rhizosphere peat soil.The abundances of three ni-trogen cycling-related bacteria,Hydrogenophaga,Sphingomonas and Nitrosomonadaceae,were also altered after treatment with the functionalized graphene.The relative abundance of Basilicum,related to soil phosphorus solubilization,decreased in the fungal com-munity,while the relative abundance of Clonostachys and Dimorphospora,which exhibited strong biological control over numerous fungal plant pathogens,nematodes and insects,increased in the soil after functionalized graphene treatment.Redundancy analysis re-vealed that the potential of hydrogen(pH),organic matter,and total phosphorus contributed the most to the changes in bacterial and fungal community composition in the rhizosphere soil.Overall,our findings suggested that the addition of functionalized graphene altered the relative abundances of nitrogen and phosphorus cycling-related microorganisms in peat soil,promoting changes in the physicochemical properties of the soil and ultimately leading to the improved growth of V.faba plants.展开更多
Exogenous jasmonic acid (JA) has been showed to be able to induce stomatal closure in Vicia faba L. in previous investigations. The transport and distribution of 3H-JA affected by localized scorch on V. faba seedl...Exogenous jasmonic acid (JA) has been showed to be able to induce stomatal closure in Vicia faba L. in previous investigations. The transport and distribution of 3H-JA affected by localized scorch on V. faba seedling were studied with radioisotope technique. The results showed that 3H-JA could be transported up or down at the rate of 4-5 cm·min -1 following feeding into root or shoot tip. The transport of 3H-JA in shoot reached a relative stable rate at 30 min after being fed through root. Wounding by scorch in the youngest leaf caused an increase in the transport of 3H-JA from root to shoot and enhanced the distribution of 3H-JA in the wounded leaf. However, distribution of 3H-JA in unwounded leaves increased after 5 h being fed through the youngest leaf. It was noticed that wounding improved accumulation of 3H-JA in abaxial epidermis. Consistent results were obtained: wounding prevented transport of 3H-JA out from the youngest leaf to root; These observations suggest that JA plays an important role as a defense signal and might be involved in the regulation of the stomatal movement in response to wounding stress.展开更多
Water channels or aquaporins are the main pathways of water transport. Both the existence and function of aquaporins in die guard cells of Vicia faba L. were investigated both by using RD28 cDNA and RD28 antibody as p...Water channels or aquaporins are the main pathways of water transport. Both the existence and function of aquaporins in die guard cells of Vicia faba L. were investigated both by using RD28 cDNA and RD28 antibody as probes, and by controlling stomatal movement as a parameter combined with antibody and inhibitor of aquaporins respectively. The results revealed that RD28 mRNA, encoding a plasma membrane aquaporin, expressed in ale mesophyll cells and vascular tissues of V. faba, especially in guard cells. And the location of RD28-like proteins was mainly on plasma membrane of guard cells. The addition of 25 mumol/L HgCl2, an aquaporin blocker, and antibody of RD28 as well, greatly suppressed the stomatal opening or guardcell protoplast swelling induced by fusicoccin and light, and closing induced by abscisic acid. However, 5 mmol/L, beta-mercaptoethanol, a reverse reagent of aquaporin blocker, reversed the inhibitory effect of HgCl2 Pretreatment oil stomatal opening ( i.e., HgCl2 was removed after HgCl2 pretreatment for 10 min). The results suggest that the aquaporins in V. faba are associated with stomatal movement.展开更多
Through field observation and lab analysis, 13 agronomic traits, including plant height and effective branching number per plant, etc., of 207 faba bean （Vicia faba L.） germplasms saved in Crop Middle-term Genebank ...Through field observation and lab analysis, 13 agronomic traits, including plant height and effective branching number per plant, etc., of 207 faba bean （Vicia faba L.） germplasms saved in Crop Middle-term Genebank of Hubei Province were evaluated, moreover, the identification on the disease resistance and the quality was carried out on parts of germplasm resources. The results showed that the faba beangermplasm resources in Hubei Province were abundant, the variation range of agronomic traits like the growth period, plant height and 100-bean weight, etc., was great, and the range of choice was wide. In the aspect of quality, there were 9 germplasms withthe content of crude protein higher than 30%, and the variation range of amylose content in total starch was large, which provided excellent sources of parents for breedinq hi.qh-quality faba bean varieties in Hubei Province.展开更多
This study was conducted to compare leaf anatomical structures and ap- parent plant print characteristics of 3 Vicia plants from Changbai Mountains applying structural botany principle and fingerprint identification t...This study was conducted to compare leaf anatomical structures and ap- parent plant print characteristics of 3 Vicia plants from Changbai Mountains applying structural botany principle and fingerprint identification technology, aiming at dis- cussing the structure evolution law of Vicia plants and providing scientific reference for research about classification, genetic relationship and evolutionary trend of this genus. The comparison of leaf anatomical structures of Vicia lilacina, V. pseu- dorobus and V. unijuga showed that： V. unijuga has tidily arranged leaf epidermal cells, thickened outer tangential wall cuticle, large diameter of xylem vessels, and developed midrib fibrocytes which form vascular bundle sheaths. Plant print identifi- cation results showed that the tested 3 plants showed remarkable differences in leaf apparent texture and anticlinal wall type though plant print characteristics of appar- ent structures of plants in the same genus had homologous similarity. V. lilacina has no subsidiary cells, and apparent textures of V. pseudorobus and V. unijuga are both of paracytic unequal type. Statistical analysis showed that V. unijuga is more advanced than V. pseudorobus. The 3 tested plants are in evolutionary rela- tionship of V. lilacina〈V, pseudorobus〈V, unijuga.展开更多
With 12 Vicia faba L. varieties of different genotypes as the research ma- terials, the contents of 9 kinds of quality factors of protein, fat, free amino acid as well as beneficial mineral elements were analyzed with...With 12 Vicia faba L. varieties of different genotypes as the research ma- terials, the contents of 9 kinds of quality factors of protein, fat, free amino acid as well as beneficial mineral elements were analyzed with the aim to provide the nec- essary theoretical basis for the selection of new varieties of high quality faba beans. The results showed that in the faba beans of different genotypes, the protein content ranged from 27.9% to 31.2%, fat content of 1.2-1.4%, tannin content of 4 700-5 280 mg/kg, iron content of 47.45-67.15 mgJkg, calcium content of 571.30-1 397.22 mg/kg, zinc content of 47.34-66.10 mg/kg, potassium content of 1.09-1.38%, and selenium content ranged from 0.031 mg/kg to 0.064 mg/kg. There were significant differences in the contents of different free amino acids between different genotypes, and the total free amino acid content was between 1.062% and 0.915%.展开更多
文摘The effect of functionalized graphene on the growth and development of Vicia faba L.was investigated by analyzing its impact on the composition and diversity of the microbial community in rhizosphere peat soil.Seedlings of V.faba planted in this peat soil were treated with either distilled water(CK)or 25 mg·L^(−1)(G25)of functionalized graphene solution.Results showed that the height and root length of V.faba seedlings in the G25 group were significantly larger than those in CK group.The microbial com-munity was analyzed by amplifying and sequencing the 16S rRNA gene V_(3)-V_(4) region of bacteria and internal transcribed spacer re-gion of fungi in rhizosphere soil using Illumina MiSeq technology.Alpha and beta diversity analysis indicated that functionalized graphene increased the richness and diversity of bacteria and fungi in the V.faba rhizosphere peat soil.The abundances of three ni-trogen cycling-related bacteria,Hydrogenophaga,Sphingomonas and Nitrosomonadaceae,were also altered after treatment with the functionalized graphene.The relative abundance of Basilicum,related to soil phosphorus solubilization,decreased in the fungal com-munity,while the relative abundance of Clonostachys and Dimorphospora,which exhibited strong biological control over numerous fungal plant pathogens,nematodes and insects,increased in the soil after functionalized graphene treatment.Redundancy analysis re-vealed that the potential of hydrogen(pH),organic matter,and total phosphorus contributed the most to the changes in bacterial and fungal community composition in the rhizosphere soil.Overall,our findings suggested that the addition of functionalized graphene altered the relative abundances of nitrogen and phosphorus cycling-related microorganisms in peat soil,promoting changes in the physicochemical properties of the soil and ultimately leading to the improved growth of V.faba plants.
文摘Exogenous jasmonic acid (JA) has been showed to be able to induce stomatal closure in Vicia faba L. in previous investigations. The transport and distribution of 3H-JA affected by localized scorch on V. faba seedling were studied with radioisotope technique. The results showed that 3H-JA could be transported up or down at the rate of 4-5 cm·min -1 following feeding into root or shoot tip. The transport of 3H-JA in shoot reached a relative stable rate at 30 min after being fed through root. Wounding by scorch in the youngest leaf caused an increase in the transport of 3H-JA from root to shoot and enhanced the distribution of 3H-JA in the wounded leaf. However, distribution of 3H-JA in unwounded leaves increased after 5 h being fed through the youngest leaf. It was noticed that wounding improved accumulation of 3H-JA in abaxial epidermis. Consistent results were obtained: wounding prevented transport of 3H-JA out from the youngest leaf to root; These observations suggest that JA plays an important role as a defense signal and might be involved in the regulation of the stomatal movement in response to wounding stress.
文摘Water channels or aquaporins are the main pathways of water transport. Both the existence and function of aquaporins in die guard cells of Vicia faba L. were investigated both by using RD28 cDNA and RD28 antibody as probes, and by controlling stomatal movement as a parameter combined with antibody and inhibitor of aquaporins respectively. The results revealed that RD28 mRNA, encoding a plasma membrane aquaporin, expressed in ale mesophyll cells and vascular tissues of V. faba, especially in guard cells. And the location of RD28-like proteins was mainly on plasma membrane of guard cells. The addition of 25 mumol/L HgCl2, an aquaporin blocker, and antibody of RD28 as well, greatly suppressed the stomatal opening or guardcell protoplast swelling induced by fusicoccin and light, and closing induced by abscisic acid. However, 5 mmol/L, beta-mercaptoethanol, a reverse reagent of aquaporin blocker, reversed the inhibitory effect of HgCl2 Pretreatment oil stomatal opening ( i.e., HgCl2 was removed after HgCl2 pretreatment for 10 min). The results suggest that the aquaporins in V. faba are associated with stomatal movement.
文摘Through field observation and lab analysis, 13 agronomic traits, including plant height and effective branching number per plant, etc., of 207 faba bean （Vicia faba L.） germplasms saved in Crop Middle-term Genebank of Hubei Province were evaluated, moreover, the identification on the disease resistance and the quality was carried out on parts of germplasm resources. The results showed that the faba beangermplasm resources in Hubei Province were abundant, the variation range of agronomic traits like the growth period, plant height and 100-bean weight, etc., was great, and the range of choice was wide. In the aspect of quality, there were 9 germplasms withthe content of crude protein higher than 30%, and the variation range of amylose content in total starch was large, which provided excellent sources of parents for breedinq hi.qh-quality faba bean varieties in Hubei Province.
基金Supported by Natural Science Foundation of China(41271231)~~
文摘This study was conducted to compare leaf anatomical structures and ap- parent plant print characteristics of 3 Vicia plants from Changbai Mountains applying structural botany principle and fingerprint identification technology, aiming at dis- cussing the structure evolution law of Vicia plants and providing scientific reference for research about classification, genetic relationship and evolutionary trend of this genus. The comparison of leaf anatomical structures of Vicia lilacina, V. pseu- dorobus and V. unijuga showed that： V. unijuga has tidily arranged leaf epidermal cells, thickened outer tangential wall cuticle, large diameter of xylem vessels, and developed midrib fibrocytes which form vascular bundle sheaths. Plant print identifi- cation results showed that the tested 3 plants showed remarkable differences in leaf apparent texture and anticlinal wall type though plant print characteristics of appar- ent structures of plants in the same genus had homologous similarity. V. lilacina has no subsidiary cells, and apparent textures of V. pseudorobus and V. unijuga are both of paracytic unequal type. Statistical analysis showed that V. unijuga is more advanced than V. pseudorobus. The 3 tested plants are in evolutionary rela- tionship of V. lilacina〈V, pseudorobus〈V, unijuga.
基金Supported by the China Agriculture Research System(CARS-09)~~
文摘With 12 Vicia faba L. varieties of different genotypes as the research ma- terials, the contents of 9 kinds of quality factors of protein, fat, free amino acid as well as beneficial mineral elements were analyzed with the aim to provide the nec- essary theoretical basis for the selection of new varieties of high quality faba beans. The results showed that in the faba beans of different genotypes, the protein content ranged from 27.9% to 31.2%, fat content of 1.2-1.4%, tannin content of 4 700-5 280 mg/kg, iron content of 47.45-67.15 mgJkg, calcium content of 571.30-1 397.22 mg/kg, zinc content of 47.34-66.10 mg/kg, potassium content of 1.09-1.38%, and selenium content ranged from 0.031 mg/kg to 0.064 mg/kg. There were significant differences in the contents of different free amino acids between different genotypes, and the total free amino acid content was between 1.062% and 0.915%.
