[ Objective] To understand the physicochemical properties of musk deer pneumonia and purulent disease viruses. [ Method] The pneu- monia and purulent disease viruses were isolated from the abnormal and purulent lung t...[ Objective] To understand the physicochemical properties of musk deer pneumonia and purulent disease viruses. [ Method] The pneu- monia and purulent disease viruses were isolated from the abnormal and purulent lung tissues of musk deer. Then the isolated viruses were inocula- ted into the Vero cells. After culturing, the virus solution was collected and used to determine TCID50 and genoma types. The sensitivity to fat sol- vent, resistance to hydrochloric acid and trypsin as well as tolerance to heat of the musk deer pneumonia and purulent disease viruses were detec- ted, respectively. [ Result] The obvious cytopathic effects （CPE） were found in Veto cells infected by the isolated viruses. The virus was 2-1.43 TCID50/ml and its genome was RNA. The virus was not sensitive to chloroform, 1% trypsin and heats, and it had a certain tolerance to 0.1 mol/L hy- drochloric acid. [ Conclusion] The study on the physicochemical properties of musk deer pneumonia and purulent disease viruses lays a foundation for prevention and control of the musk deer pneumonia and purulent diseases.展开更多
目的分析肺炎链球菌(SP)肺炎、呼吸道合胞病毒(RSV)肺炎患儿外周血25羟基维生素D(25-OHVD)、T淋巴细胞亚群、B淋巴细胞以及自然杀伤(NK)细胞的变化。方法对入院60例儿童行多病原联合检测,包括直接免疫荧光法检测7种病毒抗原、痰细菌培...目的分析肺炎链球菌(SP)肺炎、呼吸道合胞病毒(RSV)肺炎患儿外周血25羟基维生素D(25-OHVD)、T淋巴细胞亚群、B淋巴细胞以及自然杀伤(NK)细胞的变化。方法对入院60例儿童行多病原联合检测,包括直接免疫荧光法检测7种病毒抗原、痰细菌培养、聚合酶链反应(PCR)检测痰中人类偏肺病毒和博卡病毒、酶联免疫吸附测定(ELISA)法检测血清肺炎支原体IgM抗体,明确SP、RSV感染,并用流式细胞仪检测部分患儿外周血T淋巴细胞亚群、B淋巴细胞以及NK细胞,ELISA法进行25-OH-VD水平的测定。并以30例同龄健康儿童作为对照组。结果 RSV肺炎患儿25-OH-VD、CD8+T细胞及NK细胞比对照组低,分别为:46.20(42.85)nmol/L vs 68.25(19.23)nmol/L,(16.63±4.56)%vs(22.04±3.40)%,6.70(7.00)%vs 13.65(5.10)%(均P<0.05);但CD4+T细胞、B淋巴细胞CD19+较对照组高,分别为:(42.58±6.88)%vs(36.20±3.04)%,(28.25±8.85)%vs(19.38±5.76)%(均P<0.05)。SP肺炎患儿25-OH-VD及NK细胞比对照组低,分别为:61.80(15.70)nmol/L vs 68.25(19.23)nmol/L,6.25(7.57)%vs 13.65(5.10)%(均P<0.05);B淋巴细胞CD19+较对照组高,(29.70±9.72)%vs(19.38±5.76)%(P<0.05)。两感染组比较,RSV组CD4+T细胞升高,而CD8+T细胞明显下降。结论 SP、RSV感染的发生与维生素D缺乏密切相关。RSV感染后刺激机体产生细胞免疫和体液免疫反应,而SP感染后则产生以体液免疫为主的免疫反应。展开更多
目的建立甲型流感病毒(influenza A viruses,IAV)感染后继发肺炎链球菌(Streptococcus pneumoniae,Spn)感染性中耳炎小鼠模型。方法经鼻感染不同剂量甲型H1N1型流感病毒PR8,感染后第5天听泡穿刺接种Spn建立C57BL/6小鼠继发感染中耳炎模...目的建立甲型流感病毒(influenza A viruses,IAV)感染后继发肺炎链球菌(Streptococcus pneumoniae,Spn)感染性中耳炎小鼠模型。方法经鼻感染不同剂量甲型H1N1型流感病毒PR8,感染后第5天听泡穿刺接种Spn建立C57BL/6小鼠继发感染中耳炎模型,观察小鼠体质量变化和生存率;于建模后不同时间点处死小鼠,制备中耳组织切片HE染色,收集中耳灌洗液进行细胞及细菌计数,ELISA检测灌洗液中炎症因子。结果 PR8预感染可导致Spn感染性中耳炎小鼠死亡,而单纯Spn感染小鼠无死亡,小鼠体质量变化及生存率与病毒剂量呈剂量依赖方式;PR8感染后继发Spn感染小鼠中耳腔炎症细胞浸润明显多于单纯Spn感染小鼠,且细菌载量明显增加;PR8继发Spn感染小鼠中耳腔炎症因子IL-6、IFN-γ与单独Spn感染小鼠差异无统计学意义(P>0.05),而TNF-α明显增高(P<0.05)。结论成功建立了IAV感染后继发Spn感染性中耳炎C57BL/6小鼠模型。展开更多
基金supported by the Youth Fund Projects of Sichuan Education Department(07ZB060)Technology Support Projects of Sichuan Science and Technology Department(2009SZ0228)
文摘[ Objective] To understand the physicochemical properties of musk deer pneumonia and purulent disease viruses. [ Method] The pneu- monia and purulent disease viruses were isolated from the abnormal and purulent lung tissues of musk deer. Then the isolated viruses were inocula- ted into the Vero cells. After culturing, the virus solution was collected and used to determine TCID50 and genoma types. The sensitivity to fat sol- vent, resistance to hydrochloric acid and trypsin as well as tolerance to heat of the musk deer pneumonia and purulent disease viruses were detec- ted, respectively. [ Result] The obvious cytopathic effects （CPE） were found in Veto cells infected by the isolated viruses. The virus was 2-1.43 TCID50/ml and its genome was RNA. The virus was not sensitive to chloroform, 1% trypsin and heats, and it had a certain tolerance to 0.1 mol/L hy- drochloric acid. [ Conclusion] The study on the physicochemical properties of musk deer pneumonia and purulent disease viruses lays a foundation for prevention and control of the musk deer pneumonia and purulent diseases.
文摘目的分析肺炎链球菌(SP)肺炎、呼吸道合胞病毒(RSV)肺炎患儿外周血25羟基维生素D(25-OHVD)、T淋巴细胞亚群、B淋巴细胞以及自然杀伤(NK)细胞的变化。方法对入院60例儿童行多病原联合检测,包括直接免疫荧光法检测7种病毒抗原、痰细菌培养、聚合酶链反应(PCR)检测痰中人类偏肺病毒和博卡病毒、酶联免疫吸附测定(ELISA)法检测血清肺炎支原体IgM抗体,明确SP、RSV感染,并用流式细胞仪检测部分患儿外周血T淋巴细胞亚群、B淋巴细胞以及NK细胞,ELISA法进行25-OH-VD水平的测定。并以30例同龄健康儿童作为对照组。结果 RSV肺炎患儿25-OH-VD、CD8+T细胞及NK细胞比对照组低,分别为:46.20(42.85)nmol/L vs 68.25(19.23)nmol/L,(16.63±4.56)%vs(22.04±3.40)%,6.70(7.00)%vs 13.65(5.10)%(均P<0.05);但CD4+T细胞、B淋巴细胞CD19+较对照组高,分别为:(42.58±6.88)%vs(36.20±3.04)%,(28.25±8.85)%vs(19.38±5.76)%(均P<0.05)。SP肺炎患儿25-OH-VD及NK细胞比对照组低,分别为:61.80(15.70)nmol/L vs 68.25(19.23)nmol/L,6.25(7.57)%vs 13.65(5.10)%(均P<0.05);B淋巴细胞CD19+较对照组高,(29.70±9.72)%vs(19.38±5.76)%(P<0.05)。两感染组比较,RSV组CD4+T细胞升高,而CD8+T细胞明显下降。结论 SP、RSV感染的发生与维生素D缺乏密切相关。RSV感染后刺激机体产生细胞免疫和体液免疫反应,而SP感染后则产生以体液免疫为主的免疫反应。
文摘目的建立甲型流感病毒(influenza A viruses,IAV)感染后继发肺炎链球菌(Streptococcus pneumoniae,Spn)感染性中耳炎小鼠模型。方法经鼻感染不同剂量甲型H1N1型流感病毒PR8,感染后第5天听泡穿刺接种Spn建立C57BL/6小鼠继发感染中耳炎模型,观察小鼠体质量变化和生存率;于建模后不同时间点处死小鼠,制备中耳组织切片HE染色,收集中耳灌洗液进行细胞及细菌计数,ELISA检测灌洗液中炎症因子。结果 PR8预感染可导致Spn感染性中耳炎小鼠死亡,而单纯Spn感染小鼠无死亡,小鼠体质量变化及生存率与病毒剂量呈剂量依赖方式;PR8感染后继发Spn感染小鼠中耳腔炎症细胞浸润明显多于单纯Spn感染小鼠,且细菌载量明显增加;PR8继发Spn感染小鼠中耳腔炎症因子IL-6、IFN-γ与单独Spn感染小鼠差异无统计学意义(P>0.05),而TNF-α明显增高(P<0.05)。结论成功建立了IAV感染后继发Spn感染性中耳炎C57BL/6小鼠模型。
