Regional brain structural abnormality in ischemic stroke patients:a voxel-based morphometry study

Our previous study used regional homogeneity analysis and found that activity in some brain areas of patients with ischemic stroke changed significantly. In the current study, we examined structural changes in these brain regions by taking structural magnetic resonance imaging scans of 11 ischemic stroke patients and 15 healthy participants, and analyzing the data using voxel-based morphometry. Compared with healthy participants, patients exhibited higher gray matter density in the left inferior occipital gyrus and right anterior white matter tract. In contrast, gray matter density in the right cerebellum, left precentral gyrus, right middle frontal gyrus, and left middle temporal gyrus was less in ischemic stroke patients. The changes of gray matter density in the middle frontal gyrus were negatively associated with the clin- ical rating scales of the Fugl-Meyer Motor Assessment （r = -0.609, P = 0.047） and the left middle temporal gyrus was negatively correlated with the clinical rating scales of the nervous functional deficiency scale （r = -0.737, P = 0.010）. Our findings call objectively identify the functional abnormality in some brain regions of ischemic stroke patients.

White matter abnormalities in young males with antisocial personality disorder Evidence from voxel-based morphometry-diffeomorphic anatomical registration using exponentiated lie algebra analysis

Voxel-based morphometry-diffeomorphic anatomical registration using exponentiated lie algebra analysis was used to investigate the structural characteristics of white matter in young males with antisocial personality disorder （APD） and healthy controls without APD. The results revealed that APD subjects, relative to healthy subjects, exhibited increased white matter volume in the bilateral prefrontal lobe, right insula, precentral gyrus, bilateral superior temporal gyrus, right postcentral gyrus, right inferior parietal Iobule, right precuneus, right middle occipital lobe, right parahippocampal gyrus and bilateral cingulate, and decreased volume in the middle temporal cortex and right cerebellum. The white matter volume in the medial frontal gyrus was significantly correlated with antisocial type scores on the Personality Diagnostic Questionnaire in APD subjects. These experimental findings indicate that white matter abnormalities in several brain areas may contribute to antisocial behaviors in APD subjects.

Brain structure in post-traumatic stress disorder A voxel-based morphometry analysis

This study compared the difference in brain structure in 12 mine disaster survivors with chronic post-traumatic stress disorder, 7 cases of improved post-traumatic stress disorder symptoms, and 14 controls who experienced the same mine disaster but did not suffer post-traumatic stress disorder, using the voxel-based morphometry method. The correlation between differences in brain structure and post-traumatic stress disorder symptoms was also investigated. Results showed that the gray matter volume was the highest in the trauma control group, followed by the symptoms-improved group, and the lowest in the chronic post-traumatic stress disorder group. Compared with the symptoms-improved group, the gray matter volume in the lingual gyrus of the right occipital lobe was reduced in the chronic post-traumatic stress disorder group. Compared with the trauma control group, the gray matter volume in the right middle occipital gyrus and left middle frontal gyrus was reduced in the symptoms-improved group. Compared with the trauma control group, the gray matter volume in the left superior parietal lo- bule and right superior frontal gyrus was reduced in the chronic post-traumatic stress disorder group. The gray matter volume in the left superior parietal Iobule was significantly positively correlated with the State-Trait Anxiety Inventory subscale score in the symptoms-improved group and chronic post-traumatic stress disorder group （r = 0.477, P = 0.039）. Our findings indicate that （1） chronic post-traumatic stress disorder patients have gray matter structural damage in the prefrontal lobe, oc- cipital lobe, and parietal lobe, （2） after post-traumatic stress, the disorder symptoms are improved and gray matter structural damage is reduced, but cannot recover to the trauma-control level, and （3） the superior parietal Iobule is possibly associated with chronic post-traumatic stress disorder. Post-traumatic stress disorder patients exhibit gray matter abnormalities.

Regional gray matter abnormality in hepatic myelopathy patients after transjugular intrahepatic portosystemic shunt: a voxel-based morphometry study

Hepatic myelopathy is a complication seen in patients with chronic liver failure with physiologic or iatrogenic portosystemic shunting. The main symptom is progressive lower limb dyskinesia. The role of the brain motor control center in hepatic myelopathy is unknown. This study aimed to investigate the gray matter changes in patients with hepatic myelopathy secondary to transjugular intrahepatic portosystemic shunt and to examine their clinical relevance. This was a cross-sectional study. Twenty-three liver failure patients with hepatic myelopathy(hepatic myelopathy group), 23 liver failure patients without hepatic myelopathy(non-hepatic myelopathy group) after transjugular intrahepatic portosystemic shunt, and 23 demographically matched healthy volunteers were enrolled from March 2014 to November 2016 at Xijing Hospital, Air Force Military Medical University(Fourth Military Medical University), China. High-resolution magnetization-prepared rapid gradient-echo brain imaging was acquired. Group differences in regional gray matter were assessed using voxel-based morphometry analysis. The relationship between aberrant gray matter and motor characteristics was investigated. Results demonstrated that compared with the non-hepatic myelopathy group, gray matter volume abnormalities were asymmetric, with decreased volume in the left insula(P = 0.003), left thalamus(P = 0.029), left superior frontal gyrus(P = 0.006), and right middle cingulate cortex(P = 0.021), and increased volume in the right caudate nucleus(P = 0.017), corrected with open-source software. The volume of the right caudate nucleus in the hepatic myelopathy group negatively correlated with the lower limb clinical rating of the Fugl-Meyer Assessment(r = –0.53, P = 0.01). Compared with healthy controls, patients with and without hepatic myelopathy exhibited overall increased gray matter volume in both thalami, and decreased gray matter volume in both putamen, as well as in the globus pallidus, cerebellum, and vermis. The gray matter abnormalities we found predominantly involved motor-related regions, and may be associated with motor dysfunction. An enlarged right caudate nucleus might help to predict weak lower limb motor performance in patients with preclinical hepatic myelopathy after transjugular intrahepatic portosystemic shunt. This study was approved by the Ethics Committee of Xijing Hospital, Air Force Military Medical University(Fourth Military Medical University), China(approval No. 20140227-6) on February 27, 2014.

Differences in brain structure in patients with distinct sites of chronic pain:A voxel-based morphometric analysis

A reduction in gray matter volume is common in patients with chronic back pain, and different types of pain are associated with gray matter abnormalities in distinct brain regions. To examine differ- ences in brain morphology in patients with low back pain or neck and upper back pain, we investi- gated changes in gray matter volume in chronic back pain patients having different sites of pain using voxel-based morphometry. A reduction in cortical gray matter volume was found primarily in the left postcentral gyrus and in the left precuneus and bilateral cuneal cortex of patients with low back pain. In these patients, there was an increase in subcortical gray matter volume in the bilateral putamen and accumbens, right pallidum, right caudate nucleus, and left amygdala. In upper back pain patients, reduced cortical gray matter volume was found in the left precentral and left postcen- tral cortices. Our findings suggest that regional gray matter volume abnormalities in low back pain patients are more extensive than in upper back pain patients. Subcortical gray matter volume in- creases are found only in patients with low back pain.

Volume Gain of Brainstem on Medication-Overuse Headache Using Voxel-Based Morphometry

Background： Histopathology identified the anatomical and molecular abnormalities ofbrainstem nuclei in migraine patients. However, the exact whole brainstem structural changes in vivo have not yet been identified in medication-overuse headache （MOH） transformed from migraine. The aim of this study was to investigate the regional volume changes over the whole brainstem in the MOH patients using voxel-based morphometry （VBM） in vivo.Methods： High-resolution three-dimensional structural images were obtained using a 3.0-Tesla magnetic resonance system from 36 MOH patients and 32 normal controls （NCs） who were consecutively recruited from the International Headache Center, Chinese People＇s Liberation Army General Hospital, from March 2013 to June 2016. VBM was used to assess the brainstem structural alteration in the MOH patients, and voxel-wise correlation was performed to evaluate the relationship with the clinical characteristics.Results： The brainstem region with increased volume located in the left ventrolateral periaqueductal gray （MNI coordinate： -1, -33, -8）, ventral tegmental area （MNI coordinate： 0, -22, - 12）, bilateral substantia nigra （MNI coordinate： -8, - 16, - 12, 9, - 16, - 12）, and trigeminal root entry zone （MNI coordinate： -19, -29, -31; 19, -32, -29） in MOH patients compared with NCs. The headache visual analog scale score was positively related with the left rostral ventromedial medulla （RVM） （MNI coordinate： -1, -37, -56; cluster size： 20; r = 0.602） in the MOH patients.Conclusions： The regional volume gain ofbrainstem could underlie the neuromechanism of impaired ascending and descending pathway in the MOH patients, and the left RVM volume alteration could imply the impaired tolerance ofnociceptive pain input and could be used to assess the headache disability in the MOH patients.

Voxel-Based Morphometric Study on Chinese Blind Men's Brain Structure

Many studies have shown the functional relevance of cross-modal plasticity in blind men. In order to study the changes of their brain structure, voxel-based morphometry （VBM） methods are used. The regional gray matter （GM） and white matter （WM） concentrations of magnetic resonance （MR） images from 11 blind people and 9 sighted control subjects are compared using standard VBM. Optimized VBM is also discussed to measure the absolute local volume of GM or WM. Consistent results are achieved by statistical analysis with these methods. There are distinct differences not only in visual cortex but also the sensory area, auditory area and motor area. GM concentrations in blind men significantly decreased in Brodmann 7 and 22. While in Brodmann 18 and 19, GM concentration increased. GM volumes decreased in Brodmann 3, 4, 6, 9 and 45. On the other hand, both WM concentration and volume increased in Brodmann 7. These results suggest that early visual deprivation can lead to changes in the brain structural anatomy which is consistent with the cortical cross-modal reorganization found by functional imaging. It may help to discover the relationship between the brain structural anatomy and the brain functional data of blind men at a macroscopic level from neuroimaging perspective.

Application of voxel-based morphometric method to detect brain changes in children with non-cyanotic congenital heart disease

BACKGROUND Congenital heart disease(CHD)is a cardiovascular malformation caused by abnormal heart and/or vascular development in the fetus.In children with CHD,abnormalities in the development and function of the nervous system are common.At present,there is a lack of research on the preoperative neurological development and injury in young children with non-cyanotic CHD.AIM To determine the changes in white matter,gray matter,and cerebrospinal fluid(CSF)by magnetic resonance imaging(MRI)in children with non-cyanotic CHD as compared with healthy controls.METHODS Children diagnosed with non-cyanotic CHD on ultrasonography(n=54)and healthy control subjects(n=35)were included in the study.All the subjects were aged 1-3 years.Brain MRI was performed prior to surgery for CHD.The SPM v12 software was used to calculate the volumes of the gray matter,white matter,CSF,and the whole brain(sum of the gray matter,white matter,and CSF volumes).Volume differences between the two groups were analyzed.Voxel-based morphometry was used to compare specific brain regions with statistically significant atrophy.RESULTS Compared with the control group,the study group had significantly reduced whole-brain white matter volume(P<0.05),but similar whole-brain gray matter,CSF,and whole-brain volumes(P>0.05).As compared with the healthy controls,children with non-cyanotic CHD had mild underdevelopment in the white matter of the anterior central gyrus,the posterior central gyrus,and the pulvinar.CONCLUSION Children with non-cyanotic CHD show decreased white matter volume before surgery,and this volume reduction is mainly concentrated in the somatosensory and somatic motor nerve regions.

Voxel-based morphometry(VBM)MRI analysis of gray matter in patients with occupational noise-induced hearing loss

Objective To investigate the changes of brain gray matter volume in patients with occupational noise-induced hearing loss by voxel based morphometry(VBM).Methods 16 age-and education-matched healthy controls and 42 patients with occupational noise induced hearing loss,including 27 in mild group and 15 in severe group,received MRI 3D-FSPGR sequence T1WI sagittal scan,and then underwent VBM of brain gray matter volume data analysis.Results The brain gray matter volume of the left occipitotemporal lateral gyrus,the anterior cingulate gyrus,the bilateral angular gyrus,the precuneus and the near midline area of cerebellum differed between the experimental group and the control group(P<0.01).Conclusion The volume of gray matter in specific brain areas of patients with occupational noise-induced hearing loss changed,and the effect of noise on brain structure was revealed from the perspective of imaging.

Abnormal volumetric brain morphometry and cerebral blood flow in adolescents with depression

BACKGROUND Prior research has demonstrated that the brains of adolescents with depression exhibit distinct structural alterations.However,preliminary studies have documented the pathophysiological changes in certain brain regions,such as the cerebellum,highlighting a need for further research to support the current understanding of this disease.AIM To study brain changes in depressed adolescents.METHODS This study enrolled 34 adolescents with depression and 34 age-,sex-,and education-level-matched healthy control(HC)individuals.Structural and functional alterations were identified when comparing the brains of these two participant groups through voxel-based morphometry and cerebral blood flow(CBF)analysis,respectively.Associations between identified brain alterations and the severity of depressive symptoms were explored through Pearson correlation analyses.RESULTS The cerebellum,superior frontal gyrus,cingulate gyrus,pallidum,middle frontal gyrus,angular gyrus,thalamus,precentral gyrus,inferior temporal gyrus,superior temporal gyrus,inferior frontal gyrus,and supplementary motor areas of adolescents with depression showed an increase in brain volume compared to HC individuals.These patients with depression further presented with a pronounced drop in CBF in the left pallidum(group=98,and peak t=-4.4324),together with increased CBF in the right percental gyrus(PerCG)(group=90,and peak t=4.5382).In addition,17-item Hamilton Depression Rating Scale scores were significantly correlated with the increased volume in the opercular portion of the left inferior frontal gyrus(r=-0.5231,P<0.01).CONCLUSION The right PerCG showed structural and CBF changes,indicating that research on this part of the brain could offer insight into the pathophysiological causes of impaired cognition.

Impact of Multifidus Muscle Morphometry on the Clinical Evolution of Chronic Low Back Pain

Purpose: The multifidus muscle is an important extensor muscle of the lumbar spine. It plays a major role in the stability and realization of axial rotation movements of the thoraco-lumbar spine. Its atrophy by fatty degeneration would be at the origin of the occurrence of chronic low back pain which constitutes a public health problem in Senegal. Taking into account its anatomy is essential for the etiopathogenic analysis and the treatment of low back pain. The purpose of our work was to investigate the impact of multifidus muscle morphometry on the anatomy-clinical evolution of low back pain. Material and method: this was a prospective study over a period of 30 months from November 2019 to May 2022. It involved 100 patients seen in the neurology department of Fann Hospital for chronic low back pain and who had already had a scanner falling within the criteria for low back pain. We used 3D Slicer, SPSS 20, Excel 2016 software to model and analyze the morphometric data of the multifidus muscle after physiotherapy and control lumbar scans. Results: The sex ratio was 2.23. The average age of the patients was 45 ± 7 years. On the initial CT scan, according to the Hadar classification, we noted a predominance of grade 2 with 56% in L5/S1, followed by grade 1 with 32% and grade 3 with 14%. In L4/L5, the majority of patients, 67%, had grade 1. A conflicting circumferential disc bulge with the roots predominating in L5/S1 was present in 94% of men (p-value = 0.02). Before physiotherapy, the average volume of the multifidus was 193 mm3 ± 39, after physiotherapy it was 203 mm3 ± 42 with a progression rate of 5.2%. Clinically, severe type pain had regressed from 86% before physiotherapy to 0% after physiotherapy (p-value = 0.03). Conclusion: Taking into account the morphometry of the multifidus is an essential element in the management of chronic low back pain.

Effect of layer thickness and voxel size inversion on leaf area density based on the voxel-based canopy profiling method

Voxel-based canopy profiling is commonly used to determine small-scale leaf area.Layer thickness and voxel size impact accuracy when using this method.Here,we determined the optimal combination of layer thickness and voxel size to estimate leaf area density accurately.Terrestrial LiDAR Stonex X300 was used to generate point cloud data for Masson pines(Pinus massoniana).The canopy layer was stratified into 0.10-1.00-m-thick layers,while voxel size was 0.01-0.10 m.The leaf area density of individual trees was estimated using leaf area indices for the upper,middle,and lower canopy and the overall canopy.The true leaf area index,obtained by layered harvesting,was used to verify the inversion results.Leaf area density was inverted by nine combinations of layer thickness and voxel size.The average relative accuracy and mean estimated accuracy of these combined inversion results exceeded 80%.When layer thickness was 1.00 m and voxel size 0.05 m,inversion was closest to the true value.The average relative accuracy was 92.58%,mean estimated accuracy 98.00%,and root mean square error 0.17.The combination of leaf area density and index was accurately retrieved.In conclusion,nondestructive voxel-based canopy profiling proved suitable for inverting the leaf area density of Masson pine in Hetian Town,Fujian Province.

Bull spermatozoa selected by thermotaxis exhibit high DNA integrity,specific head morphometry,and improve ICSI outcome

Background Sperm migration by thermotaxis is a guidance mechanism that operates along the oviduct and it has proved to be a valid method for selecting spermatozoa with low DNA fragmentation(SDF)in mice,humans,and stallions.This study aimed to analyse if bull spermatozoa could be selected by thermotaxis and to assess their quality in terms of SDF as well as determine the presence of a specific sperm subpopulation based on sperm morphometry and assess their fertilizing capacity by ICSI.Methods We used frozen-thawed sperm from 6 bulls and sperm selection by thermotaxis was performed with TALP medium supplemented with 25 mmol/L of HEPES and 5 mmol/L of caffeine.In these conditions,sperm selection was achieved,obtaining a net thermotaxis of 3.6%.Subsequently,we analysed the SDF of the migrated and not-migrated spermatozoa using the neutral COMET assay,and we evaluated the size of the sperm head using Hemacolor■ staining with Motic Images Plus 3 software.Additionally,migrated and not-migrated spermatozoa by thermotaxis were used to fertilize bovine in vitro matured(IVM)oocytes by ICSI,a very inefficient procedure in cattle that is only successful when the oocyte is artificially activated.Results The results showed lower SDF(χ^(2),P<0.001,13.3%reduction,n=8)and lower head size parameters(length and width,P<0.01;and perimeter and area,P<0.001;n=4)in those spermatozoa migrated in comparison to those not-migrated.The distribution of sperm subpopulations structure varied between groups,highlighting cluster 2,characterized by spermatozoa with small head size,and high ellipticity and elongated heads,as the most abundant in the thermotaxis migrated group.When performed ICSI(without oocyte artificial activation)with the thermotactic sperm,the blastocyst rate was 32.2%±9.3%in the group microinjected with the thermotactic spermatozoa vs.8.3%±7.8%in the group of not-migrated sperm(χ^(2),P<0.05).Conclusion Our results showed that bull sperm selection by thermotaxis has a much higher DNA integrity,small and elongated head size parameters,and different sperm subpopulation structure than the not-selected spermatozoa.Additionally,we evidenced that thermotactic spermatozoa improve ICSI success rates.

作物农艺性状与形态结构表型智能识别技术综述

[目的/意义]作物农艺性状与形态结构表型智能识别是作物智慧育种的主要内容,是研究“基因型—环境型—表型”相互作用关系的基础,对现代作物育种具有重要意义。[进展]大规模、高通量作物表型获取设备是作物表型获取、分析、测量、识别等的基础和重要手段。本文介绍了高通量作物表型主流平台和感知成像设备的功能、性能以及应用场景。分析了作物株高获取、作物器官检测与技术等农艺性状智能识别和作物株型识别、作物形态信息测量以及作物三维重建等形态结构智能识别技术的研究进展及挑战。[结论/展望]从研制新型低成本田间智能作物表型获取与分析装备、提升作物表型获取田间环境的标准化与一致性水平、强化田间作物表型智能识别模型的通用性,研究多视角、多模态、多点连续分析与时空特征融合的作物表型识别方法,以及提高模型解释性等方面,展望了作物表型技术主要发展方向。

Magnetic resonance morphometry of the loss of gray matter volume in Parkinson's disease patients

Voxel-based morphometry can be used to quantitatively compare structural differences and func-tional changes of gray matter in subjects. In the present study, we compared gray matter images of 32 patients with Parkinson’s disease and 25 healthy controls using voxel-based morphometry based on 3.0 T high-field magnetic resonance T1-weighted imaging and clinical neurological scale scores. Results showed that the scores in Mini-Mental State Examination and Montreal Cognitive Assessment were lower in patients compared with controls. In particular, the scores of visuospatial/executive function items in Montreal Cognitive Assessment were significantly reduced, but mean scores of non-motor symptoms significantly increased, in patients with Parkinson’s dis-ease. In addition, gray matter volume was significantly diminished in Parkinson’s disease patients compared with normal controls, including bilateral temporal lobe, bilateral occipital lobe, bilateral parietal lobe, bilateral frontal lobe, bilateral insular lobe, bilateral parahippocampal gyrus, bilateral amygdale, right uncus, and right posterior lobe of the cerebel um. These findings indicate that voxel-based morphometry can accurately and quantitatively assess the loss of gray matter volume in patients with Parkinson＇s disease, and provide essential neuroimaging evidence for multisystem pathological mechanisms involved in Parkinson’s disease.

西沙群岛鹦嘴鱼科耳石形态学物种鉴定的可行性研究

研究于2018—2021年在西沙群岛海域采集鹦嘴鱼样本,通过传统形态测量法选取的19个耳石形态指标和椭圆傅里叶分析法选取的77个椭圆傅里叶系数对其进行鉴别分析,探究耳石形态在鹦嘴鱼科物种鉴别中的可行性。结果表明,仅使用耳石形态指标鉴别鹦嘴鱼时综合判别率为56.8%,低于仅使用椭圆傅里叶系数的63.1%和两种参数结合的75.7%;而结合两种参数对其4个属的综合判别率为88.6%,其中绚鹦嘴鱼属的判别率达到100%;15种鹦嘴鱼的耳石形态聚类结果与已有研究的进化树结果基本一致,特别是属层面的聚类,呈现高度一致,证实了耳石形态具有遗传的属性,也说明了耳石形态学用于鱼类物种鉴定的可行性。研究结果将为西沙群岛鹦嘴鱼的基础研究提供科学资料,为鹦嘴鱼资源的保护与开发提供理论依据。

伴躯体症状抑郁症患者磁共振脑灰质体积异常表现

目的 采用基于体素的形态学分析(voxel-based morphometry,VBM)比较伴和不伴躯体症状抑郁症患者大脑灰质体积变化,探索伴躯体症状抑郁症患者在MRI上特有表现。方法 纳入52例抑郁症患者,根据入组时健康问卷躯体症状群量表评分是否>9分将其分为伴躯体症状组和不伴躯体症状组,同时纳入性别、年龄匹配的健康志愿者40名作为对照组。所有被试予MRI扫描,采用VBM方法比较各组大脑灰质体积的差异。结果 与对照组相比,伴躯体症状组右颞上回灰质体积增加,右眶额下回、右颞下回、左眶额下回、左颞上回体积减少(P<0.001),不伴躯体症状组右颞中回灰质体积增加,右颞上回、右颞下回体积减少(P<0.01)。抑郁症患者中,伴躯体症状组右舌回、左扣带回的体积较不伴躯体症状组增加(P<0.01)。结论 抑郁症伴躯体症状患者在VBM-MRI上表现为舌回、扣带回体积增加。

3D-T1WI表面形态学分析在学龄前无灶性癫痫患儿脑结构评估中的应用

目的:采用表面形态学分析(surface-based morphometry,SBM)技术对学龄前无灶性癫痫(non-lesional epilepsy,NLE)患儿的3D-T_(1)WI脑结构影像进行分析,评估患儿脑结构的改变。方法:选取2021年1月至2022年9月某院神经内科确诊为无灶性癫痫的35例学龄前患儿纳入NLE组,选取同时期的35例学龄前健康儿童纳入对照组。对比2组患儿的脑皮层厚度、脑沟深度、脑回指数、脑脊液体积、脑灰质体积及脑白质体积。采用SPSS 25.0统计学软件对数据进行分析。结果:与对照组相比,NLE组患儿左侧大脑半球下侧颞叶皮层厚度变薄、右侧大脑半球后扣带回皮层厚度增厚、右侧大脑半球下侧颞叶皮层厚度变薄,差异有统计学意义(P<0.05);与对照组相比,NLE组患儿右侧大脑半球舌回和额中回脑沟深度变浅,差异有统计学意义(P<0.05);与对照组相比,NLE组患儿左侧大脑半球距状回皮层脑回指数增大,差异有统计学意义(P<0.05)。2组患儿在脑脊液、脑灰质及脑白质方面比较,差异无统计学意义(P>0.05)。结论:NLE患儿的皮层结构异常可能是其致病主要因素,SBM技术对3D-T_(1)WI脑结构影像的分析结果可以评估NLE患儿局部脑结构异常,是探索儿童NLE潜在发病机制的有效工具。

上呼吸道感染后嗅觉障碍患者脑形态学与嗅觉功能相关性分析

目的 通过探究上呼吸道感染后嗅觉障碍脑灰质形态变化,得出上呼吸道感染后嗅觉障碍患者嗅觉中枢结构的影像学特点。方法 选取上呼吸道感染后嗅觉障碍的患者24例与嗅觉功能正常的健康对照受试者20例,通过基于体素的大脑皮质形态学体积测量(VBM)比较患者的大脑灰质和白质体积与对照组的差异。结果 上呼吸道感染后嗅觉障碍组患者TDI[气味察觉阈(T)、气味辨别能力(D)、气味识别能力(I)]总分(16.45±5.62)分,T为(2.33±0.93)分,D为(6.54±2.60)分,I为(7.58±3.22)分;嗅觉障碍平均时长为(19.00±6.33)个月;视觉模拟量表评分(VAS)为(7.79±2.41)分,与对照组比较均存在统计学差异(P<0.05)。上呼吸道感染后嗅觉障碍患者的大脑灰质总体积和嗅皮质体积明显小于对照组(P<0.05),大脑灰质总体积占全脑体积的比例和皮质厚度与嗅觉功能评分TDI值呈正相关(r=0.71,P<0.000 1;r=0.69,P=0.000 9)。结论 上呼吸道感染后嗅觉障碍患者大脑灰质总体积和嗅皮质体积减少,且嗅觉功能与大脑灰质体积比例及皮质厚度呈正相关。

乳腺癌化疗患者脑结构及功能磁共振成像研究

目的探讨乳腺癌化疗患者的脑灰质体积(gray matter volume,GMV)变化及静息状态下部分脑区与全脑功能连接(functional connectivity,FC)的改变。材料与方法本研究共纳入29名术后化疗前的乳腺癌患者(without chemotherapy treatment,C−)和30名化疗后乳腺癌患者(chemotherapy treatment,C+),分别进行了心理认知测试、结构及静息态功能MRI扫描,运用基于体素的形态学测量(voxel-based morphometry,VBM)方法对GMV进行分析,并选择体积变化脑区为感兴趣区(region of interest,ROI)进行全脑FC的分析。GMV比较及FC比较均采用双样本t检验分析方法。采用Spearman偏相关分析来探讨差异脑区GMV及FC值与心理认知测试结果之间的关系。结果与C−组相比,C+患者左侧枕中回(MNI:X,Y,Z=−10.5,−105.0,0.0)、右侧距状裂周围皮层(MNI:X,Y,Z=15.0,−103.5,−1.5)GMV减小(P<0.001,cluster size>90)。以左侧枕中回为种子点,与右侧颞极:颞上回(MNI:X,Y,Z=63,3,−3)、左侧颞上回(MNI:X,Y,Z=−54,−12,3)FC减弱;以右距状裂周围皮层为种子点,与左侧舌回(MNI:X,Y,Z=−33,−87,−15)FC减弱(P<0.001,FWE-corrected<0.05)。C+患者GMV与FC值与心理认知结果之间未存在明显相关性(P>0.05)。结论乳腺癌患者化疗后大脑部分区域GMV改变,与大脑多个脑区存在FC受损,这可能是其认知、情感及行为障碍的神经病理基础之一。