BACKGROUND Expression of the full-length isoform of Abelson interactor 1(ABI1),ABI1-p65,is increased in colorectal carcinoma(CRC)and is thought to be involved in one or more steps leading to tumor progression or metas...BACKGROUND Expression of the full-length isoform of Abelson interactor 1(ABI1),ABI1-p65,is increased in colorectal carcinoma(CRC)and is thought to be involved in one or more steps leading to tumor progression or metastasis.The ABI1 splice isoform-L(ABI1-SiL)has conserved WAVE2-binding and SH3 domains,lacks the homeodomain homologous region,and is missing the majority of PxxP-and Pro-rich domains found in full-length ABI1-p65.Thus,ABI1-SiL domain structure suggests that the protein may regulate CRC cell morphology,adhesion,migration,and metastasis via interactions with the WAVE2 complex pathway.AIM To investigate the potential role and underlying mechanisms associated with ABI1-SiL-mediated regulation of CRC.METHODS ABI1-SiL mRNA expression in CC tissue and cell lines was measured using both qualitative reverse transcriptase-polymerase chain reaction(RT-PCR)and realtime quantitative RT-PCR.A stably ABI1-SiL overexpressing SW480 cell model was constructed using Lipofectamine 2000,and cells selected with G418.Image J software,CCK8,and transwell assays were used to investigate SW480 cell surface area,proliferation,migration,and invasion.Immunoprecipitation,Western blot,and co-localization assays were performed to explore intermolecular interactions between ABI1-SiL,WAVE2,and ABI1-p65 proteins.RESULTS ABI1-SiL was expressed in normal colon tissue and was significantly decreased in CRC cell lines and tissues.Overexpression of ABI1-SiL in SW480 cells significantly increased the cell surface area and inhibited the adhesive and migration properties of the cells,but did not alter their invasive capacity.Similar to ABI1-p65,ABI1-SiL still binds WAVE2,and the ABI1-p65 isoform in SW480 cells.Furthermore,co-localization assays confirmed these intermolecular interactions.CONCLUSION These results support a model in which ABI1-SiL plays an anti-oncogenic role by competitively binding to WAVE2 and directly interacting with phosphorylated and non-phosphorylated ABI1-p65,functioning as a dominant-negative form of ABI1-p65.展开更多
The spatial growth of the disturbance in the boundary layer is directly numerically simulated, and the receptivity of the Blasius basic flow to the local two-dimensional （2-D） sustainable micro-vibration is investig...The spatial growth of the disturbance in the boundary layer is directly numerically simulated, and the receptivity of the Blasius basic flow to the local two-dimensional （2-D） sustainable micro-vibration is investigated. Results show that the disturbance velocity presents the sine vibration features with the change of time, and the vibration period is identical to the vibration of the local wall. The disturbance velocity presents the fluctuation feature downstream, and the streamwise wave length approximates to the results from the Orr-Sommerfeld equation （OSE）. The growth rate from direct numerical simulation（DNS） is a little greater than that from OSE, and their trends are almost consistent. Under the condition of Re= 2 800, the disturbance amplitude gradually grows in the given computational region with the period T=30. However, it firstly increases and then decreases with the period T= 20. The disturbance harmonic of the former is obviously larger than that of the latter. The maximum streamwise and vertical disturbance velocities from DNS do not fully coincide with those from OSE at the vicinity of the local vibration wall, but coincide well with the former when they travel downstream. The 2-D disturbance induced by the local micro-vibration represents the form of Tollmien-Schlichting （T-S） wave on the boundary layer.展开更多
Numerical simulations are carried out to investigate the mechanism of the nonlinear evolution of two- dimensional （2-D） Tollmien-Schlichting （T-S） wave on a localized rough boundary layer. The three-dimensional （...Numerical simulations are carried out to investigate the mechanism of the nonlinear evolution of two- dimensional （2-D） Tollmien-Schlichting （T-S） wave on a localized rough boundary layer. The three-dimensional （3-D） numerical solution of a base flow on a boundary layer is obtained for the localized rough wall with the local- ized ejection, the localized suction and the combination of ejection and suction. Based on numerical simulations, the processes of stable and the most instable nonlinear evolution of the 2-D disturbance T-S wave are studied. The effects of the form on the localized roughness, the intensity, and the distribution structure on the nonlinear evolution of 2-D T-S wave and the growth rate are discussed. Results show that the basic flow induced by the lo- calized rough wall is a key factor causing the fast growth of the disturbance wave. Due to the change of the aver- age flow profile and the existence of the spanwise velocity, the localized rough wall enhances the instability of the flow. Consequently, the instable region of the neutral curve is enlargened, and the maximnum growth rate of the 2-D T-S wave is increased. In the process of the nonlinear evolution of 2-D disturbance T-S wave, with the in- crease of the nonlinear interaction, the most instable 2-D disturbance wave triggers the appearance of the 3-D dis- turbance wave and the high-frequency harmonic wave. Its streamwise wave number and the frequency are the same as those of 2-D disturbance wave. The spanwise velocity can excite the growth of the 2-D disturbance wave, the instability of 2-D wave, the formation of the streamwise vortex, and the generation of 3-D disturbance wave. Simulation results agree well with experimental results.展开更多
Typhoon-generated waves are simulated with two numerical wave models, the SWAN model for the coastal and Yangtze Estuary domain, nested within the WAVEWATCHIII （WW3） for the basin-scale East China Sea domain. Typhoo...Typhoon-generated waves are simulated with two numerical wave models, the SWAN model for the coastal and Yangtze Estuary domain, nested within the WAVEWATCHIII （WW3） for the basin-scale East China Sea domain. Typhoon No. 8114 is chosen because it was very strong, and generated high waves in the Estuary. WW3 was implemented for the East China Sea coarse-resolution computational domain, to simulate the waves over a large spatial scale and provide boundary conditions for SWAN model simulations, implemented on a fine-resolution nested domain for the Yangtze Estuary area. The Takahashi wind model is applied to the simulation of the East China Sea scale （3-hourly） and Yangtze Estuary scale （1-hourly） winds. Simulations of significant wave heights in the East China Sea show that the highest waves are on the right side of the storm track, and maxima tend to occur at the eastern deep-water open boundary of the Yangtze Estuary. In the Yangtze Estuary, incoming swell is dominant over locally generated waves before the typhoon approaches the Estuary. As the typhoon approaches the Estuary, wind waves and swell coexist, and the wave direction is mainly influenced by the swell direction and the complex topography.展开更多
In this study, the coupled heave-pitch motion equations of a spar platform were established by considering lst-order and 2nd-order random wave loads and the effects of time-varying displacement volume and transient wa...In this study, the coupled heave-pitch motion equations of a spar platform were established by considering lst-order and 2nd-order random wave loads and the effects of time-varying displacement volume and transient wave elevation. We generated random wave loads based on frequency-domain wave load transfer functions and the Joint North Sea Wave Project （JONSWAP） wave spectrum, designed program codes to solve the motion equations, and then simulated the coupled heave-pitch motion responses of the platform in the time domain. We then calculated and compared the motion responses in different sea conditions and separately investigated the effects of 2nd-order random wave loads and transient wave elevation. The results show that the coupled heave-pitch motion responses of the platform are primarily dominated by wave height and the characteristic wave period, the latter of which has a greater impact. 2nd-order mean wave loads mainly affect the average heave value. The platform＇s pitch increases after the 2nd-order low frequency wave loads are taken into account. The platform＇s heave is underestimated if the transient wave elevation term in the motion equations is neglected.展开更多
The mode-2 internal solitary waves(ISWs)generated by mode-2 internal tide(IT)are identified by mooring observations in the northern South China Sea(SCS)from 2016 to 2017.Two mode-2 ISWs with a re-appearance period of ...The mode-2 internal solitary waves(ISWs)generated by mode-2 internal tide(IT)are identified by mooring observations in the northern South China Sea(SCS)from 2016 to 2017.Two mode-2 ISWs with a re-appearance period of 24.9 h observed on 29 and 30 July 2016 are characterized by type-b ISWs.They occurred when the isotherms compressed obviously in the vertical direction.Modal decomposition of IT horizontal currents shows that the vertical compression of the isotherms is mainly caused by diurnal mode-2 IT.The analysis of the role of the density stratification reveals that a deeper and thinner pycnocline is favorable for generation of mode-2 ISWs rather than pycnocline intensity.By comparing the mode-2 nonlinear,dispersion coefficients and the Ursell numbers calculated based on the stratification associated with different kinds of ITs with the observation results,it is shown that the diurnal mode-2 IT plays a crucial role in the generation of the mode-2 ISWs.When the diurnal mode-2 IT interacts with the semidiurnal IT and causes a deeper and thinner pycnocline,the mode-2 ISWs are easily excited.展开更多
In the process of 2-D compressional wave propagation in a rock mass with multiple parallel joints along the radian direction normal to the joints, the maximum possible wave amplitude corresponding to the points betwee...In the process of 2-D compressional wave propagation in a rock mass with multiple parallel joints along the radian direction normal to the joints, the maximum possible wave amplitude corresponding to the points between the two adjacent joints in the joint set is controlled by superposition of the multiple transmitted and the reflected waves, measured by the maximum rebound ratio. Parametric studies on the maximum rebound ratio along the radian direction normal to the joints were performed in universal distinct element code. The results show that the maximum rebound ratio is influenced by three factors, i.e., the normalized normal stiffness of joints, the ratio of joint spacing to wavelength and the joint from which the wave rebounds. The relationship between the maximum rebound ratio and the influence factors is generalized into five charts. Those charts can be used as the prediction model for estimating the maximum rebound ratio.展开更多
Based on the Tang-Othmer Ca^2＋ model, the drift behavior of intracellular Ca^2＋ spiral waves under the influence of weak electric field is investigated. Numerical results show that the dependence of drift velocity o...Based on the Tang-Othmer Ca^2＋ model, the drift behavior of intracellular Ca^2＋ spiral waves under the influence of weak electric field is investigated. Numerical results show that the dependence of drift velocity of the spiral tip on dc electric field is similar to experimental observations in BZ system. When an ac electric field is applied, interesting resonant-drift phenomenon is observed with ω=2ω0. All results can be explained analytically using a proximate method.展开更多
The two-dimensional （2D） nonlinear Rossby waves described by the Petviashvili equation, which has been invoked as an ageostrophic extension of the barotropic quasi-geostrophic potential vorticity equation, can be in...The two-dimensional （2D） nonlinear Rossby waves described by the Petviashvili equation, which has been invoked as an ageostrophic extension of the barotropic quasi-geostrophic potential vorticity equation, can be investigated through the exact periodic-wave solutions for the Petviashvili equation, while the exact analytical periodic-wave solutions to the Petviashvili equation are obtained by using the Jacobi elliptic function expansion method. It is shown that periodicwave 2D Rossby solutions can be obtained by this method, and in the limit cases, the 213 Rossby soliton solutions are also obtained.展开更多
基金National Natural Science Foundation of China,No.30872923 and No.81672853and Peking University People’s Hospital Scientific Research Development Found,No.RDH2020-11.
文摘BACKGROUND Expression of the full-length isoform of Abelson interactor 1(ABI1),ABI1-p65,is increased in colorectal carcinoma(CRC)and is thought to be involved in one or more steps leading to tumor progression or metastasis.The ABI1 splice isoform-L(ABI1-SiL)has conserved WAVE2-binding and SH3 domains,lacks the homeodomain homologous region,and is missing the majority of PxxP-and Pro-rich domains found in full-length ABI1-p65.Thus,ABI1-SiL domain structure suggests that the protein may regulate CRC cell morphology,adhesion,migration,and metastasis via interactions with the WAVE2 complex pathway.AIM To investigate the potential role and underlying mechanisms associated with ABI1-SiL-mediated regulation of CRC.METHODS ABI1-SiL mRNA expression in CC tissue and cell lines was measured using both qualitative reverse transcriptase-polymerase chain reaction(RT-PCR)and realtime quantitative RT-PCR.A stably ABI1-SiL overexpressing SW480 cell model was constructed using Lipofectamine 2000,and cells selected with G418.Image J software,CCK8,and transwell assays were used to investigate SW480 cell surface area,proliferation,migration,and invasion.Immunoprecipitation,Western blot,and co-localization assays were performed to explore intermolecular interactions between ABI1-SiL,WAVE2,and ABI1-p65 proteins.RESULTS ABI1-SiL was expressed in normal colon tissue and was significantly decreased in CRC cell lines and tissues.Overexpression of ABI1-SiL in SW480 cells significantly increased the cell surface area and inhibited the adhesive and migration properties of the cells,but did not alter their invasive capacity.Similar to ABI1-p65,ABI1-SiL still binds WAVE2,and the ABI1-p65 isoform in SW480 cells.Furthermore,co-localization assays confirmed these intermolecular interactions.CONCLUSION These results support a model in which ABI1-SiL plays an anti-oncogenic role by competitively binding to WAVE2 and directly interacting with phosphorylated and non-phosphorylated ABI1-p65,functioning as a dominant-negative form of ABI1-p65.
基金Supported by the National Natural Science Foundation of China(10672052)the Advanced TalentStart-Up Foundation of Jiangsu University(08JDG018)~~
文摘The spatial growth of the disturbance in the boundary layer is directly numerically simulated, and the receptivity of the Blasius basic flow to the local two-dimensional （2-D） sustainable micro-vibration is investigated. Results show that the disturbance velocity presents the sine vibration features with the change of time, and the vibration period is identical to the vibration of the local wall. The disturbance velocity presents the fluctuation feature downstream, and the streamwise wave length approximates to the results from the Orr-Sommerfeld equation （OSE）. The growth rate from direct numerical simulation（DNS） is a little greater than that from OSE, and their trends are almost consistent. Under the condition of Re= 2 800, the disturbance amplitude gradually grows in the given computational region with the period T=30. However, it firstly increases and then decreases with the period T= 20. The disturbance harmonic of the former is obviously larger than that of the latter. The maximum streamwise and vertical disturbance velocities from DNS do not fully coincide with those from OSE at the vicinity of the local vibration wall, but coincide well with the former when they travel downstream. The 2-D disturbance induced by the local micro-vibration represents the form of Tollmien-Schlichting （T-S） wave on the boundary layer.
基金Supported by the National Natural Science Foundation of China(10872097)the Natural Science Foundation of Jiangsu Province(BK2007178)Science Foundation of Nanjing University Information Science & Technology(20080101)~~
文摘Numerical simulations are carried out to investigate the mechanism of the nonlinear evolution of two- dimensional （2-D） Tollmien-Schlichting （T-S） wave on a localized rough boundary layer. The three-dimensional （3-D） numerical solution of a base flow on a boundary layer is obtained for the localized rough wall with the local- ized ejection, the localized suction and the combination of ejection and suction. Based on numerical simulations, the processes of stable and the most instable nonlinear evolution of the 2-D disturbance T-S wave are studied. The effects of the form on the localized roughness, the intensity, and the distribution structure on the nonlinear evolution of 2-D T-S wave and the growth rate are discussed. Results show that the basic flow induced by the lo- calized rough wall is a key factor causing the fast growth of the disturbance wave. Due to the change of the aver- age flow profile and the existence of the spanwise velocity, the localized rough wall enhances the instability of the flow. Consequently, the instable region of the neutral curve is enlargened, and the maximnum growth rate of the 2-D T-S wave is increased. In the process of the nonlinear evolution of 2-D disturbance T-S wave, with the in- crease of the nonlinear interaction, the most instable 2-D disturbance wave triggers the appearance of the 3-D dis- turbance wave and the high-frequency harmonic wave. Its streamwise wave number and the frequency are the same as those of 2-D disturbance wave. The spanwise velocity can excite the growth of the 2-D disturbance wave, the instability of 2-D wave, the formation of the streamwise vortex, and the generation of 3-D disturbance wave. Simulation results agree well with experimental results.
基金This project is supported bythe Canadian Panel on Energy Research and Development (Offshore Environmental Fac-tors Program) , ONR (US Office of Naval Research) via GoMOOS-the Gulf of Maine Ocean Observing System,Petroleum Research Atlantic Canada (PRAC) ,and the CFCAS (Canada Foundation for Climate and AtmosphericStudies) ,Canadian Panel on Energy Research and Development (Offshore Environmental Factors Program) .It is al-so supported bythe Advanced Doctoral Fund of the Ministry of Education of China (Grant No.20030294010)
文摘Typhoon-generated waves are simulated with two numerical wave models, the SWAN model for the coastal and Yangtze Estuary domain, nested within the WAVEWATCHIII （WW3） for the basin-scale East China Sea domain. Typhoon No. 8114 is chosen because it was very strong, and generated high waves in the Estuary. WW3 was implemented for the East China Sea coarse-resolution computational domain, to simulate the waves over a large spatial scale and provide boundary conditions for SWAN model simulations, implemented on a fine-resolution nested domain for the Yangtze Estuary area. The Takahashi wind model is applied to the simulation of the East China Sea scale （3-hourly） and Yangtze Estuary scale （1-hourly） winds. Simulations of significant wave heights in the East China Sea show that the highest waves are on the right side of the storm track, and maxima tend to occur at the eastern deep-water open boundary of the Yangtze Estuary. In the Yangtze Estuary, incoming swell is dominant over locally generated waves before the typhoon approaches the Estuary. As the typhoon approaches the Estuary, wind waves and swell coexist, and the wave direction is mainly influenced by the swell direction and the complex topography.
基金Foundation item： Supported by the National Natural Science Foundation of China under Grant No. 51279130 and No. 51239008
文摘In this study, the coupled heave-pitch motion equations of a spar platform were established by considering lst-order and 2nd-order random wave loads and the effects of time-varying displacement volume and transient wave elevation. We generated random wave loads based on frequency-domain wave load transfer functions and the Joint North Sea Wave Project （JONSWAP） wave spectrum, designed program codes to solve the motion equations, and then simulated the coupled heave-pitch motion responses of the platform in the time domain. We then calculated and compared the motion responses in different sea conditions and separately investigated the effects of 2nd-order random wave loads and transient wave elevation. The results show that the coupled heave-pitch motion responses of the platform are primarily dominated by wave height and the characteristic wave period, the latter of which has a greater impact. 2nd-order mean wave loads mainly affect the average heave value. The platform＇s pitch increases after the 2nd-order low frequency wave loads are taken into account. The platform＇s heave is underestimated if the transient wave elevation term in the motion equations is neglected.
基金The National Science and Technology Major Project under contract No.2016ZX05057015the National Natural Science Foundation of China(NSFC)under contract Nos 41376038+6 种基金4040600941806123 and 41506038the NSFC-Shandong Joint Fund for Marine Science Research Centers under contract No.U1606405the National Program on Global Change and Air-Sea Interaction under contract Nos GASI-03-01-01-02GASI-02-IND-STSsum and GASI-IPOVAI-01-05the Public Science and Technology Research Funds Projects of Ocean under contract No.200905024the National Key Scientific Instrument and Equipment Development Projects under contract No.2012YQ12003908。
文摘The mode-2 internal solitary waves(ISWs)generated by mode-2 internal tide(IT)are identified by mooring observations in the northern South China Sea(SCS)from 2016 to 2017.Two mode-2 ISWs with a re-appearance period of 24.9 h observed on 29 and 30 July 2016 are characterized by type-b ISWs.They occurred when the isotherms compressed obviously in the vertical direction.Modal decomposition of IT horizontal currents shows that the vertical compression of the isotherms is mainly caused by diurnal mode-2 IT.The analysis of the role of the density stratification reveals that a deeper and thinner pycnocline is favorable for generation of mode-2 ISWs rather than pycnocline intensity.By comparing the mode-2 nonlinear,dispersion coefficients and the Ursell numbers calculated based on the stratification associated with different kinds of ITs with the observation results,it is shown that the diurnal mode-2 IT plays a crucial role in the generation of the mode-2 ISWs.When the diurnal mode-2 IT interacts with the semidiurnal IT and causes a deeper and thinner pycnocline,the mode-2 ISWs are easily excited.
基金Projects(50278057) supported by the National Natural Science Foundation of China project(2002CB412703) supported by Major State Basic Research Development Program of China
文摘In the process of 2-D compressional wave propagation in a rock mass with multiple parallel joints along the radian direction normal to the joints, the maximum possible wave amplitude corresponding to the points between the two adjacent joints in the joint set is controlled by superposition of the multiple transmitted and the reflected waves, measured by the maximum rebound ratio. Parametric studies on the maximum rebound ratio along the radian direction normal to the joints were performed in universal distinct element code. The results show that the maximum rebound ratio is influenced by three factors, i.e., the normalized normal stiffness of joints, the ratio of joint spacing to wavelength and the joint from which the wave rebounds. The relationship between the maximum rebound ratio and the influence factors is generalized into five charts. Those charts can be used as the prediction model for estimating the maximum rebound ratio.
基金Supported by National Natural Science Foundation of China under Grant Nos.10575041 and 10747005
文摘Based on the Tang-Othmer Ca^2＋ model, the drift behavior of intracellular Ca^2＋ spiral waves under the influence of weak electric field is investigated. Numerical results show that the dependence of drift velocity of the spiral tip on dc electric field is similar to experimental observations in BZ system. When an ac electric field is applied, interesting resonant-drift phenomenon is observed with ω=2ω0. All results can be explained analytically using a proximate method.
基金The project supported by National Natural Science Foundation of China under Grant No. 40475023
文摘The two-dimensional （2D） nonlinear Rossby waves described by the Petviashvili equation, which has been invoked as an ageostrophic extension of the barotropic quasi-geostrophic potential vorticity equation, can be investigated through the exact periodic-wave solutions for the Petviashvili equation, while the exact analytical periodic-wave solutions to the Petviashvili equation are obtained by using the Jacobi elliptic function expansion method. It is shown that periodicwave 2D Rossby solutions can be obtained by this method, and in the limit cases, the 213 Rossby soliton solutions are also obtained.
